Assuntos
Variação Genética , Sarampo/diagnóstico , Técnicas de Diagnóstico Molecular/normas , Reação em Cadeia da Polimerase Via Transcriptase Reversa/normas , Primers do DNA/genética , Monitoramento Epidemiológico , Reações Falso-Negativas , França , Humanos , Sarampo/virologia , Vírus do Sarampo/genética , Vírus do Sarampo/isolamento & purificação , Técnicas de Diagnóstico Molecular/métodos , Polimorfismo de Nucleotídeo Único , RNA Viral/genética , Kit de Reagentes para Diagnóstico/normas , Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos , Transcrição Reversa , Sensibilidade e EspecificidadeRESUMO
BACKGROUND: In France, mumps surveillance is conducted in primary care by the Sentinelles network, the National Reference Centre for Measles, Mumps and Rubella and Santé publique France. AIM: The objective of this study was to estimate the incidence of suspected mumps in general practice, the proportion of laboratory confirmed cases and the factors associated with a virological confirmation. METHODS: General practitioners (GPs) participating in the Sentinelles network should report all patients with suspected mumps according to a clinical definition in case of parotitis and a serological definition in case of clinical expression without parotitis. All suspected mumps cases reported between January 2014 and December 2020 were included. A sample of these cases were tested by real time reverse transcriptase polymerase chain reaction (RT-PCR) for mumps biological confirmation. RESULTS: A total of 252 individuals with suspected mumps were included in the study. The average annual incidence rate of suspected mumps in general practice in France between 2014 and 2020 was estimated at 11 cases per 100,000 population [CI95%: 6-17]. A mumps confirmation RT-PCR test was performed on 146 cases amongst which 17 (11.5 %) were positive. Age (between 20 and 29 years old), the presence of a clinical complication and an exposure to a suspected mumps case within the 21 days prior the current episode were associated with a mumps biological confirmation. CONCLUSION: If these results confirm the circulation of mumps virus in France, they highlight the limits of a surveillance without a systematic laboratory confirmation in highly immunized populations.
Assuntos
Medicina Geral , Caxumba , Parotidite , Humanos , Adulto Jovem , Adulto , Caxumba/diagnóstico , Caxumba/epidemiologia , Caxumba/prevenção & controle , Parotidite/epidemiologia , Vírus da Caxumba , França/epidemiologia , Vacina contra Sarampo-Caxumba-RubéolaRESUMO
Measles outbreaks are regularly reported in European countries despite efforts to improve vaccination coverage. In January 2016, an outbreak occurred in a refugee settlement in Calais, France. We report here the complete genome sequence of a wild-type measles virus isolated from a health care worker (MVi/Calais. FRA/01.16) infected during this outbreak.