1.
Nat Commun
; 8: 14568, 2017 02 21.
Artigo
em Inglês
| MEDLINE
| ID: mdl-28220771
RESUMO
Here we report the expansion of the genetic code of Mus musculus with various unnatural amino acids including NÉ-acetyl-lysine. Stable integration of transgenes encoding an engineered NÉ-acetyl-lysyl-tRNA synthetase (AcKRS)/tRNAPyl pair into the mouse genome enables site-specific incorporation of unnatural amino acids into a target protein in response to the amber codon. We demonstrate temporal and spatial control of protein acetylation in various organs of the transgenic mouse using a recombinant green fluorescent protein (GFPuv) as a model protein. This strategy will provide a powerful tool for systematic in vivo study of cellular proteins in the most commonly used mammalian model organism for human physiology and disease.