RESUMO
PM2.5 (particulate matter with an aerodynamic diameter <2.5 microm) samples (n = 58) collected every sixth day in Xi'an, China, from 5 July 2008 to 27 June 2009 are analyzed for levoglucosan (1,6-anhydro-beta-D-glucopyranose) to evaluate the impacts of biomass combustion on ambient concentrations. Twenty-four-hour levoglucosan concentrations displayed clear summer minima and winter maxima that ranged from 46 to 1889 ng m(-3), with an average of 428 +/- 399 ng m(-3). Besides agricultural burning, biomass/biofuel combustion for household heating with straws and branches appears to be of regional importance during the heating season in northwestern China. Good correlations (0.70 < R < 0.91) were found between levoglucosan relative to water- soluble K+, Cl-, organic carbon (OC), elemental carbon (EC), and glyoxal. The highest levoglucosan/OC ratio of2.3% wasfound in winter, followed by autumn (1.5%). Biomass burning contributed to 5.1-43.8% of OC (with an average of 17.6 +/- 8.4%).
Assuntos
Poluentes Atmosféricos/química , Monitoramento Ambiental/métodos , Glucose/análogos & derivados , Material Particulado/química , China , Incêndios , Glucose/química , Tamanho da Partícula , Estações do AnoRESUMO
UNLABELLED: PM2.5 in 14 of China's large cities achieves high concentrations in both winter and summer with averages > 100 microg m(-3) being common occurrences. A grand average of 15 microg m(-3) was found for all cities, with a minimum of 27 microg m(-3) measured at Qingdao during summer and a maximum of 356 microg m(-3) at Xi 'an during winter. Both primary and secondary PM2.5 are important contributors at all of the cities and during both winter and summer. While ammonium sulfate is a large contributor during both seasons, ammonium nitrate contributions are much larger during winter. Lead levels are still high in several cities, reaching an average of 1.68 microg m(-3) in Xi 'an. High correlations of lead with arsenic and sulfate concentrations indicate that much of it derives from coal combustion, rather than leaded fuels, which were phased out by calendar year 2000. Although limited fugitive dust markers were available, scaling of iron by its ratios in source profiles shows -20% of PM2.5 deriving from fugitive dust in most of the cities. Multipollutant control strategies will be needed that address incomplete combustion of coal and biomass, engine exhaust, and fugitive dust, as well as sulfur dioxide, oxides of nitrogen, and ammonia gaseous precursors for ammonium sulfate and ammonium nitrate. IMPLICATIONS: PM2.5 mass and chemical composition show large contributions from carbon, sulfate, nitrate, ammonium, and fugitive dust during winter and summer and across fourteen large cities. Multipollutant control strategies will be needed that address both primary PM2.5 emissions and gaseous precursors to attain China's recently adopted PM2.5 national air quality standards.
Assuntos
Poluentes Atmosféricos/análise , Material Particulado/análise , China , Cidades , Monitoramento Ambiental , Tamanho da Partícula , Estações do Ano , Espectrometria por Raios XRESUMO
OBJECTIVE: To observe the effect of electroacupuncture (EA) at "Zusanli"(ST36) on the phosphorylated tau levels in pancreas and hippocampus of type 2 diabetes mellitus (T2DM) ratsï¼so as to explore the underlying mechanism of EA in diabetic demention rats. METHODS: Forty-eight male Sprague-Dawley rats were randomly divided into control, model and EA groups, with 16 rats in each group. The T2DM model was established by 6 weeks of high-fat, high-sugar diet as well as intrape-ritoneal injection of streptozocin (STZ) solution (35 mg/kg). After that, EA (2 Hz, 0.1 mA) was applied to unilateral "Zusanli"(ST36) for 30 min, once a day, 6 times a week for 4 weeks. The survival rate was recorded every week, and the fasting blood glucose (FBG) was detected on the 1st, 6th and 11th week. The level of serum insulin (INS) was measured by using ELISA. The morphological structure of pancreas islet was observed by H.E. staining. The expressions of phosphorylated tau at the sites of Ser 396 (pS396) and Thr 231 (pT231), total tau (Tau5), phosphorylated glycogen synthase kinase-3ß (pGSK-3ß) and total glycogen synthase kinase-3ß (GSK-3ß) in pancreas and hippocampus were detected by Western blot. The expression and distribution of pS396 and pT231 in pancreas and hippocampus were assayed with immunohistochemistry. RESULTS: Compared with the control group, the survival rate presented a significant decline, the contents of FBG and INS were obviously higher(P<0.01), and the structure of the pancreas islet appeared shrunken, obscure and disordered in the model group. Furthermore, the levels of pS396, pT231 in pancreas and hippocampus were obviously higher in the model group(P<0.01)ï¼while the level of pGSK-3ß in pancreas and hippocampus was significantly lower in the model group(P<0.01). In comparison with the model group, the survival rate of EA group was higher. Following 4 weeks' interventions, the enhanced levels of tau phosphorylation and GSK-3ß activity in pancreas and hippocampus were partly reversed in the EA group compared to the model group(P<0.05ï¼P<0.01). CONCLUSION: EA at ST36 can reduce the level of tau phosphorylation via regulating the activity of GSK-3ß in the pancreas and hippocampus of T2DM rats, which may be related with the effect of EA on the brain function in T2DM rats.
Assuntos
Diabetes Mellitus Experimental , Diabetes Mellitus Tipo 2 , Eletroacupuntura , Ilhotas Pancreáticas , Animais , Diabetes Mellitus Experimental/genética , Diabetes Mellitus Experimental/terapia , Glicogênio Sintase Quinase 3 beta/genética , Hipocampo , Masculino , Ratos , Ratos Sprague-DawleyRESUMO
PM2.5 samples were collected in Xi'an, China during haze(2015-11-30-2015-12-9) and clean days(2016-01-13-2016-01-22). PM2.5 mass, organic carbon(OC), elemental carbon(EC), and water-soluble ions(i.e., NH4+, NO3-, and SO42-) were measured to investigate the characteristics of the two typical pollution processes. The result showed that PM2.5 masses were(170±47.5) µg·m-3 and(48.6±17.9) µg·m-3 for the haze and clean days, respectively, with the haze accompanied by low visibility, high humidity, calm wind, and other adverse weather conditions. The secondary inorganic aerosol(SIA) including NH4+, NO3-, and SO42- accounted for 49.8%±13.1% and 19.4%±5.95% of the PM2.5 mass for the haze and clean days, respectively. sulfur and nitrogen oxidation ratios(SOR and NOR) on haze days were 0.282±0.157 and 0.269±0.124, respectively, which were much higher than those measured on the clean days(0.189±0.057 and 0.077±0.046). The average concentration of secondary organic carbon(SOC) for the haze days(6.22±3.87) µg·m-3 was 5 times of that measured in the clean days(1.44±1.63) µg·m-3. Secondary pollution and adverse weather conditions were the main reasons for the heavy haze. Finally, the level of particulate-Reactive Oxygen Species(ROS)was measured by the 2',7'-dichlorofluorescin diacetate(2',7'-DCFH) fluorescence method, and the average ROS concentration (as H2O2) was(4.99±1.54) nmol·m-3 during haze days and(0.492±0.356) nmol·m-3 during clean days. Accumulation effect and secondary oxidation process may be the main reasons for the increase of ROS concentration under the heavy haze conditions in Xi'an. Positive correlations(P<0.05) between the concentrations of ROS and the ions of NO3- and SO42- indicated that ROS may participate in secondary oxidation process of SIA.
RESUMO
To study the characteristics and sources of carbonaceous aerosol in PM2.5 during winter in Guanzhong area, PM2.5 samples were collected from December 2012 to February 2013 in Xi'an, Baoji, Weinan and Qinling, and then organic carbon (OC) and elemental carbon (EC) were analyzed following the thermal/optical reflection protocol. The average concentrations of OC in the four sites were 47.8, 45.8, 31.2 and 37.0 microg x m(-3), respectively, while EC concentrations were 8.5, 6.7, 7.6 and 5.7 microg x m(-3), respectively. Total carbonaceous aerosol (TCA) accounted for 36.4%, 46.2%, 36.9% and 33.4% of PM2.5, respectively. OC was strongly correlated with EC in Xi'an (R2 = 0.93) and Qinling (R2 = 0.91), while weakly correlated in Baoji (R2 = 0.58) and Weinan (R2 = 0.62), which indicated that OC and EC had more similar sources or higher mixing degree in the former two sites. All OC/EC ratios exceeded 2.0, which indicated the formation of secondary organic carbon (SOC). In Xi'an, Baoji, Weinan and Qinling, SOC accounted for 21.6%, 40.3%, 23.2% and 27.8% of OC, respectively. Positive matrix factorization (PMF) was used to analyze the sources of carbonaceous aerosol and four sources were obtained. Coal burning was the major source, contributing 45.3%-47.9% in Guanzhong area. Gasoline vehicle and biomass burning were the minor sources, contributing 26.1%-33.1% and 14.3%-20.1% respectively. In addition, diesel vehicle also had some contribution to carbonaceous aerosol.
Assuntos
Aerossóis/análise , Poluentes Atmosféricos/análise , Monitoramento Ambiental , Material Particulado/análise , Estações do Ano , Biomassa , Carbono/análise , China , Carvão Mineral , Gasolina , Tamanho da PartículaRESUMO
Enhanced neurogenesis has been reported in the hippocampus of patients with Alzheimer's disease (AD), the most common neurodegenerative disorder characterized with amyloid-ß (Aß) aggregation, tau hyperphosphorylation, and progressive neuronal loss. Previously we reported that tau phosphorylation played an essential role in adult hippocampal neurogenesis, and activation of glycogen synthase kinase (GSK-3), a crucial tau kinase, could induce increased hippocampal neurogenesis. In the present study, we found that treatment of D-galactose rats with Puerarin could significantly improve behavioral performance and ameliorate the enhanced neurogenesis and microtubule-associated protein tau hyperphosphorylation in the hippocampus of D-galactose rat brains. FGF-2/GSK-3 signaling pathway might be involved in the effects of Puerarin on hippocampal neurogenesis and tau hyperphosphorylation. Our finding provides primary in vivo evidence that Puerarin can attenuate AD-like enhanced hippocampal neurogenesis and tau hyperphosphorylation. Our finding also suggests Puerarin can be served as a treatment for age-related neurodegenerative disorders, such as AD.
Assuntos
Galactose/toxicidade , Hipocampo/efeitos dos fármacos , Isoflavonas/farmacologia , Neurogênese/efeitos dos fármacos , Fármacos Neuroprotetores/farmacologia , Proteínas tau/metabolismo , Animais , Transtornos Cognitivos/tratamento farmacológico , Transtornos Cognitivos/patologia , Transtornos Cognitivos/fisiopatologia , Modelos Animais de Doenças , Fator 2 de Crescimento de Fibroblastos/metabolismo , Quinase 3 da Glicogênio Sintase/metabolismo , Hipocampo/patologia , Hipocampo/fisiopatologia , Masculino , Aprendizagem em Labirinto/efeitos dos fármacos , Aprendizagem em Labirinto/fisiologia , Neurogênese/fisiologia , Neurônios/efeitos dos fármacos , Neurônios/patologia , Neurônios/fisiologia , Fosforilação/efeitos dos fármacos , Distribuição Aleatória , Ratos Sprague-Dawley , Transdução de Sinais/efeitos dos fármacos , Memória Espacial/efeitos dos fármacos , Memória Espacial/fisiologiaRESUMO
Exposure to particulate matter less than 2.5 µm (PM2.5) in size is an urgent issue for the protection of human health. Chemicals with PM2.5 collected during a period of intensive haze episodes in Beijing (BJ), Xian (XA) and Hong Kong (HK) were characterised for organic carbon (OC), elemental carbon (EC), total carbon (TC) and free amino acids. BALB/c mice underwent aspiration exposure of 50 or 150 µg of PM2.5/mouse (BJ, XA and HK) on days 1 and 7 and were euthanised on day 14. The effects of these exposures on E-cadherin and inflammatory responses in the mouse lungs were analysed. The PM2.5 chemicals consisted of significant amounts of OC: 36.6 ± 17.2 µg/m(3) for BJ, 38.8 ± 3.8 µg/m(3) for XA and 7.2 ± 1.4 µg/m(3) for HK. A total of 23 free amino compounds for the PM2.5 samples were analysed: 4075 ± 1578 pmol/m(3) for BJ, 4718 ± 2190 pmol/m(3) for XA and 1145 ± 213 pmol/m(3) for HK. Exposure to PM2.5 resulted in the suppression of E-cadherin levels in the lung tissues and increased IFN-γ, IL-2, IL-4, IL-6 and IL-10 in the bronchoalveolar lavage fluid. The alterations in E-cadherin, IFN-γ, IL-6 and IL-10 were associated with OC, TC and some amino acids, particularly non-protein-type amino acids. These data emphasised the deleterious health effects of PM2.5.
Assuntos
Aminoácidos/metabolismo , Caderinas/metabolismo , Carbono/química , Inflamação/patologia , Material Particulado/toxicidade , Animais , Líquido da Lavagem Broncoalveolar/química , Regulação para Baixo , Feminino , Inflamação/induzido quimicamente , Interferon gama/metabolismo , Interleucina-10/metabolismo , Interleucina-2/metabolismo , Interleucina-4/metabolismo , Interleucina-6/metabolismo , Pulmão/efeitos dos fármacos , Pulmão/patologia , Camundongos , Camundongos Endogâmicos BALB CRESUMO
PM2.5 mass and selected chemical species are measured in 24-h integrated PM2.5 samples collected simultaneously at the urban and rural regions of Xi'an (six sites in total), China in the four seasons of 2010. The analytes include organic carbon and elemental carbon (OC+EC = total carbon, TC), seven water-soluble inorganic ions (NH4(+), K(+), Mg(2+), Ca(2+), Cl(-), SO4(2-), NO3(-)) and six trace elements (Ti, Mn, Fe, Zn, As, Pb). The average PM2.5 mass for the entire measurement period is 142.6 ± 102.7 µg m(-3), which is more than four times that of the Chinese national ambient air quality standard. Spatial variations in PM2.5 mass are not pronounced. The PM2.5 mass and those species measured show a similar seasonal pattern in all six measurement sites, i.e., in the order of winter > autumn > spring > summer. The dominant PM2.5 composition is OC in winter, soil dust in spring, and sulfate, nitrate, and ammonium in summer and autumn. Seasonal variations of TC/PM2.5 and OC/EC ratios follow the PM2.5 changes. Seasonal distributions of (SO4(2-)+NO3(-)+NH4(+))/PM2.5 showed increase in autumn and decrease in winter, while NO3(-)/SO4(2-) ratios increased in autumn and decreased in summer. Eight main PM2.5 sources are identified based on the positive matrix factorization (PMF) analysis and emissions from fossil fuel combustion (traffic and coal burning) are founded to be the main source responsible for the fine particle pollution in Xi'an. In addition, a decreasing trend in OC/PM2.5 is observed in comparison with previous studies in Xi'an.
Assuntos
Poluentes Atmosféricos/análise , Poluição do Ar/estatística & dados numéricos , Monitoramento Ambiental , Material Particulado/análise , China , Estações do AnoRESUMO
The concentrations of PM2.5 carbon fractions in rural, urban, tunnel and remote environments were measured using the IMPROVE thermal optical reflectance (TOR) method. The highest OC1 and EC1 concentrations were found for tunnel samples, while the highest OC2, OC3, and OC4 concentrations were observed for urban winter samples, respectively. The lowest levels of most carbon fractions were found for remote samples. The percentage contributions of carbon fractions to total carbon (TC) were characterized by one peak (at rural and remote sites) and two peaks (at urban and tunnel sites) with different carbon fractions, respectively. The abundance of char in tunnel and urban environments was observed, which might partly be due to traffic-related tire-wear. Various percentages of optically scattering OC and absorbing EC fractions to TC were found in the four different environments. In addition, the contribution of heating carbon fractions (char and soot) indicated various warming effects per unit mass of TC. The ratios of OC/EC and char/soot at the sites were shown to be source indicators. The investigation of carbon fractions at different sites may provide some information for improving model parameters in estimating their radiative effects.
RESUMO
The potential effects of inhaled fine particulate matter (PM2.5), found in haze episodes, on the oxidation of the proteins in the lungs are not well understood. We investigated the effects of PM2.5 from haze episodes on protein oxidation. PM2.5 was collected from the air pollution in Beijing (BJ), Xian (XA), Xiamen (XM) and Hong Kong (HK) during a period of intensive haze episodes. The chemical characteristics of these samples and their effects on albumin oxidation were investigated. The levels of PM2.5 in BJ and XA were 4-6 times higher than in XM and HK. The concentrations of the polycyclic aromatic hydrocarbons (PAHs) components of the PM2.5 from BJ and XA were 10 times higher than those found in XM and HK. The haze PM2.5 increased oxidative stress. Addition of PM2.5 samples collected from haze episodes to albumin in vitro resulted in oxidation of methionine moieties; nasal instillation of PM2.5 suspensions in mice resulted in oxidation of methionine in the albumin in the bronchoalveolar lavage fluid. The methionine moieties participate in peptide chain crosslinking, and methionine oxidation in the albumin could be attributed to the PAH compounds. Our findings may be helpful in explaining the potential respiratory effects during haze episodes.