Visual learning in tethered bees modifies flight orientation and is impaired by epinastine.

Visual-orientation learning of a tethered flying bee was investigated using a flight simulator and a novel protocol in which orientation preference toward trained visual targets was assessed in tests performed before and after appetitive conditioning. Either a blue or a green rectangle (conditioned stimulus, CS) was associated with 30% sucrose solution (unconditioned stimulus, US), whereas the other rectangle was not paired with US. Bees were tested in a closed-looped flight simulator 5 min after ten pairings of the US and CS. Conditioned bees were preferentially oriented to the CS after such training. This increase in preference for CS was maintained for 24 h, indicating the presence of long-term memory. Because the total orienting time was not altered by conditioning, conditioning did not enhance orientation activity itself but increased the relative time for orientation to CS. When 0.4 or 4 mM epinastine (an antagonist of octopamine receptors) was injected into the bee's head 30 min prior to the experiment, both short- and long-term memory formation were significantly impaired, suggesting that octopamine, which is crucial for appetitive olfactory learning in insects, is also involved in visual orientation learning.

Experimental Demonstration of Direct Path State Characterization by Strongly Measuring Weak Values in a Matter-Wave Interferometer.

A method was recently proposed and experimentally realized for characterizing a quantum state by directly measuring its complex probability amplitudes in a particular basis using so-called weak values. Recently, Vallone and Dequal [Phys. Rev. Lett. 116, 040502 (2016)PRLTAO0031-900710.1103/PhysRevLett.116.040502] showed theoretically that weak measurements are not a necessary condition to determine the weak value. Here, we report a measurement scheme used in a matter-wave interferometric experiment in which the neutron path system's quantum state was characterized via direct measurements, using both strong and weak interactions. Experimental evidence is given that strong interactions outperform weak ones for tomographic accuracy. Our results are not limited to neutron interferometry, but can be used in a wide range of quantum systems.

Experimental Test of Residual Error-Disturbance Uncertainty Relations for Mixed Spin-½ States.

The indeterminacy inherent in quantum measurements is an outstanding character of quantum theory, which manifests itself typically in the uncertainty principle. In the last decade, several universally valid forms of error-disturbance uncertainty relations were derived for completely general quantum measurements for arbitrary states. Subsequently, Branciard established a form that is optimal for spin measurements for some pure states. However, the bound in his inequality is not stringent for mixed states. One of the present authors recently derived a new bound tight in the corresponding mixed state case. Here, a neutron-optical experiment is carried out to investigate this new relation: it is tested whether error and disturbance of quantum measurements disappear or persist in mixing up the measured ensemble. The attainability of the new bound is experimentally observed, falsifying the tightness of Branciard's bound for mixed spin states.

Erratum: Experimental Test of Entropic Noise-Disturbance Uncertainty Relations for Spin-1/2 Measurements [Phys. Rev. Lett. 115, 030401 (2015)].

This corrects the article DOI: 10.1103/PhysRevLett.115.030401.

Experimental Test of Entropic Noise-Disturbance Uncertainty Relations for Spin-1/2 Measurements.

Information-theoretic definitions for noise and disturbance in quantum measurements were given in [Phys. Rev. Lett. 112, 050401 (2014)] and a state-independent noise-disturbance uncertainty relation was obtained. Here, we derive a tight noise-disturbance uncertainty relation for complementary qubit observables and carry out an experimental test. Successive projective measurements on the neutron's spin-1/2 system, together with a correction procedure which reduces the disturbance, are performed. Our experimental results saturate the tight noise-disturbance uncertainty relation for qubits when an optimal correction procedure is applied.

Subglottic laryngeal closure: a unique modified method of laryngotracheal separation to prevent aspiration.

OBJECTIVES: Laryngotracheal separation (LTS) is an ideal surgical method for intractable aspiration; however, the oral side of the tracheal stump can easily disintegrate. Therefore, we developed a modified LTS method. We performed subglottic laryngeal closure (SGLC) as a new surgical method and evaluated the outcomes. METHODS: We retrospectively analyzed the medical records of 36 patients (28 male and 8 female; 15 to 91 years of age) who underwent SGLC between 2007 and 2011 at Tottori University Hospital, Japan. Operative data (operative time, intraoperative bleeding, and time to drain removal), outcomes (aspiration and changes in nutritional status), and complications with regard to the surgical method were examined. The occurrence of a subcutaneous proximal laryngeal stump fistula was evaluated by videofluoroscopy. RESULTS: The SGLC was performed safely in all patients. Fistulization was observed in only 1 of the patients (2.8%), and major bleeding after surgery was observed in 1 patient (2.8%). The procedure relieved aspiration pneumonia in all patients. CONCLUSIONS: We conclude that SGLC is effective for treating and preventing pulmonary aspiration. The incidence of postoperative complications, particularly that of subcutaneous fistulas, was very low. Therefore, this method may be useful for patients in poor condition.

Quantum causality emerging in a delayed-choice quantum Cheshire Cat experiment with neutrons.

We report an experiment with neutrons in a silicon perfect crystal interferometer, that realizes a quantum Cheshire Cat in a delayed choice setting. In our setup the quantum Cheshire Cat is established by spatially separating the particle and its property (i.e. the neutron and its spin) into the two different paths of the interferometer. The condition for a delayed choice setting is achieved by postponing the choice of path assignment for the quantum Cheshire Cat, i.e. which path is taken by the particle and which by its property, until the point in time when the neutron wave function has already split and entered the interferometer. The results of the experiment suggest not only the fact that the neutrons and its spin are separated and take different paths in the interferometer, but also quantum-mechanical causality is implied, insomuch that the behavior of a quantum system is affected by the choice of the selection at a later point in time.

Biohydrogen production by mixed culture of Megasphaera elsdenii with lactic acid bacteria as Lactate-driven dark fermentation.

Numerous attempts have been made to upscale biohydrogen production via dark fermentation (DF); however, the Achilles' heel of DF, i.e., lactic acid bacteria (LAB) contamination and overgrowth, hinders such upscaling. Key microbes are needed to develop a lactate-driven DF system that can serve as a lactate fermentation platform. In this study, the utility of Megasphaera elsdenii and LAB co-culturing in lactate-driven DF was evaluated. When inoculated simultaneously with LAB or after LAB culture, M. elsdenii achieved a stable hydrogen yield of 0.95-1.49 H2-mol/mol-glucose, approximately half that obtained in pure M. elsdenii cultures. Hydrogen production was maintained even at an initial M. elsdenii-to-LAB cell ratio of one-millionth or less. Moreover, M. elsdenii produced hydrogen via lactate-driven DF from unusable sugars such as xylose or cellobiose. Thus, M. elsdenii could be a Game changer instrumental in unlocking the full potential of DF.

Violation of a Bell-like inequality in single-neutron interferometry.

Non-local correlations between spatially separated systems have been extensively discussed in the context of the Einstein, Podolsky and Rosen (EPR) paradox and Bell's inequalities. Many proposals and experiments designed to test hidden variable theories and the violation of Bell's inequalities have been reported; usually, these involve correlated photons, although recently an experiment was performed with (9)Be(+) ions. Nevertheless, it is of considerable interest to show that such correlations (arising from quantum mechanical entanglement) are not simply a peculiarity of photons. Here we measure correlations between two degrees of freedom (comprising spatial and spin components) of single neutrons; this removes the need for a source of entangled neutron pairs, which would present a considerable technical challenge. A Bell-like inequality is introduced to clarify the correlations that can arise between observables of otherwise independent degrees of freedom. We demonstrate the violation of this Bell-like inequality: our measured value is 2.051 +/- 0.019, clearly above the value of 2 predicted by classical hidden variable theories.

Spatial Non-Cyclic Geometric Phase in Neutron Interferometry.

We present a split-beam neutron interferometric experiment to test the non-cyclic geometric phase tied to the spatial evolution of the system: the subjacent two-dimensional Hilbert space is spanned by the two possible paths in the interferometer and the evolution of the state is controlled by phase shifters and absorbers. A related experiment was reported previously by some of the authors to verify the cyclic spatial geometric phase. The interpretation of this experiment, namely to ascribe a geometric phase to this particular state evolution, has met severe criticism. The extension to non-cyclic evolution manifests the correctness of the interpretation of the previous experiment by means of an explicit calculation of the non-cyclic geometric phase in terms of paths on the Bloch-sphere. The theoretical treatment comprises the cyclic geometric phase as a special case, which is confirmed by experiment.

Treatment Outcomes of Head and Neck Squamous Cell Carcinoma in the Elderly: A Retrospective Study over 7 Years (2003-2009).

BACKGROUND: Head and neck squamous cell carcinoma (HNSCC) is increasing in prevalence as society ages worldwide. However, there are no established treatment protocols for elderly patients, and the threshold for defining "elderly" is undetermined. In this study, we categorized elderly patients (65 years and older) with HNSCC into 2 groups: "young-old," from 65 to 74 years old, and "old-old," 75 years and older, and compared their treatment outcomes. METHODS: The subjects were 182 patients aged 65 years and older who visited our hospital for HNSCC from 2003 to 2009. We categorized them into 2 groups, young-old (65-74 years) and old-old (75 years and older), and compared the male-female ratio, ratio with underlying diseases, primary tumor sites, disease stage, applied treatments and curative rate. Additionally, for the curative treatment category in both groups, we compared recurrence rate, relationship between recurrence rate and use of concomitant chemotherapy, the 5-year relapse-free survival and the 5-year cause-specific survival. RESULTS: The ratio of patients with underlying diseases in the old-old group was significantly higher than in the young-old group, but there was no significant difference in curative rate between the 2 (old-old, 81.9%; young-old, 82.7%). The 5-year, cause-specific survival in curative treatment category was significantly lower in the old-old (66.1%) group than the young-old (94.1%) group. CONCLUSION: Elderly patients of all ages should positively receive curative treatment. We suppose that concomitant chemotherapy is not acceptable in elderly patients. The 5-CSS of the curative treatment category in the old-old patients was significantly lower than in the young-old patients.

Observation of a quantum Cheshire Cat in a matter-wave interferometer experiment.

From its very beginning, quantum theory has been revealing extraordinary and counter-intuitive phenomena, such as wave-particle duality, Schrödinger cats and quantum non-locality. Another paradoxical phenomenon found within the framework of quantum mechanics is the 'quantum Cheshire Cat': if a quantum system is subject to a certain pre- and postselection, it can behave as if a particle and its property are spatially separated. It has been suggested to employ weak measurements in order to explore the Cheshire Cat's nature. Here we report an experiment in which we send neutrons through a perfect silicon crystal interferometer and perform weak measurements to probe the location of the particle and its magnetic moment. The experimental results suggest that the system behaves as if the neutrons go through one beam path, while their magnetic moment travels along the other.

Effect of Olfactory Stimulus on the Flight Course of a Honeybee, Apis mellifera, in a Wind Tunnel.

It is known that the honeybee, Apis mellifera, uses olfactory stimulus as important information for orienting to food sources. Several studies on olfactory-induced orientation flight have been conducted in wind tunnels and in the field. From these studies, optical sensing is used as the main information with the addition of olfactory signals and the navigational course followed by these sensory information. However, it is not clear how olfactory information is reflected in the navigation of flight. In this study, we analyzed the detailed properties of flight when oriented to an odor source in a wind tunnel. We recorded flying bees with a video camera to analyze the flight area, speed, angular velocity and trajectory. After bees were trained to be attracted to a feeder, the flight trajectories with or without the olfactory stimulus located upwind of the feeder were compared. The results showed that honeybees flew back and forth in the proximity of the odor source, and the search range corresponded approximately to the odor spread area. It was also shown that the angular velocity was different inside and outside the odor spread area, and trajectories tended to be bent or curved just outside the area.

How do honeybees attract nestmates using waggle dances in dark and noisy hives?

It is well known that honeybees share information related to food sources with nestmates using a dance language that is representative of symbolic communication among non-primates. Some honeybee species engage in visually apparent behavior, walking in a figure-eight pattern inside their dark hives. It has been suggested that sounds play an important role in this dance language, even though a variety of wing vibration sounds are produced by honeybee behaviors in hives. It has been shown that dances emit sounds primarily at about 250-300 Hz, which is in the same frequency range as honeybees' flight sounds. Thus the exact mechanism whereby honeybees attract nestmates using waggle dances in such a dark and noisy hive is as yet unclear. In this study, we used a flight simulator in which honeybees were attached to a torque meter in order to analyze the component of bees' orienting response caused only by sounds, and not by odor or by vibrations sensed by their legs. We showed using single sound localization that honeybees preferred sounds around 265 Hz. Furthermore, according to sound discrimination tests using sounds of the same frequency, honeybees preferred rhythmic sounds. Our results demonstrate that frequency and rhythmic components play a complementary role in localizing dance sounds. Dance sounds were presumably developed to share information in a dark and noisy environment.

Kochen-Specker theorem studied with neutron interferometer.

The Kochen-Specker theorem shows the incompatibility of noncontextual hidden variable theories with quantum mechanics. Quantum contextuality is a more general concept than quantum non-locality which is quite well tested in experiments using Bell inequalities. Within neutron interferometry we performed an experimental test of the Kochen-Specker theorem with an inequality, which identifies quantum contextuality, by using spin-path entanglement of single neutrons. Here entanglement is achieved not between different particles, but between degrees of freedom of a single neutron, i.e., between spin and path degree of freedom. Appropriate combinations of the spin analysis and the position of the phase shifter allow an experimental verification of the violation of an inequality derived from the Kochen-Specker theorem. The observed violation 2.291±0.008≰1 clearly shows that quantum mechanical predictions cannot be reproduced by noncontextual hidden variable theories.

Proposed experiment for testing quantum contextuality with neutrons.

We show that an experimental demonstration of quantum contextuality using 2 degrees of freedom of single neutrons based on a violation of an inequality derived from the Peres-Mermin proof of the Kochen-Specker theorem would be more conclusive than those obtained from previous experiments involving pairs of ions [M. A. Rowe, Nature (London) 409, 791 (2001)10.1038/35057215] and single neutrons [Y. Hasegawa, Nature (London) 425, 45 (2003)10.1038/nature01881] based on violations of Clauser-Horne-Shimony-Holt-like inequalities.

Observation of nonadditive mixed-state phases with polarized neutrons.

In a neutron polarimetry experiment the mixed-state relative phases between spin eigenstates are determined from the maxima and minima of measured intensity oscillations. We consider evolutions leading to purely geometric, purely dynamical, and combined phases. It is experimentally demonstrated that the sum of the individually determined geometric and dynamical phases is not equal to the associated total phase which is obtained from a single measurement, unless the system is in a pure state.

Quantum contextuality in a single-neutron optical experiment.

An experimental demonstration of quantum contextuality with neutrons is presented, which intended to exhibit a Kochen-Specker-like phenomenon. Since no perfect correlation is expected in practical experiments, inequalities are derived to distinguish quantitatively the obtained results from predictions by a noncontextual hidden variable theory. Experiments were accomplished with the use of a neutron interferometer combined with spinor manipulation devices. The results clearly violate the prediction of noncontextual theories.