Molecular diagnostics of the HBB gene in an Omani cohort using bench-top DNA Ion Torrent PGM technology.

Hemoglobinopathies, such as sickle cell disease (SCD) and beta-thalassemia major (TM), are severe diseases and the most common autosomal recessive condition worldwide and in particular in Oman. Early screening and diagnosis of carriers are the key for primary prevention. Once a country-wide population screening program is mandated by law, a sequencing technology that can rapidly confirm or identify disease-causing mutations for a large number of patients in a short period of time will be necessary. While Sanger sequencing is the standard protocol for molecular diagnosis, next generation sequencing starts to become available to reference laboratories. Using the Ion Torrent PGM sequencer, we have analyzed a cohort of 297 unrelated Omani cases and reliably identified mutations in the beta-globin (HBB) gene. Our model study has shown that Ion Torrent PGM can rapidly sequence such a small gene in a large number of samples using a barcoded uni-directional or bi-directional sequence methodology, reducing cost, workload and providing accurate diagnosis. Based on our results we believe that the Ion Torrent PGM sequencing platform, able to analyze hundreds of patients simultaneously for a single disease gene can be a valid molecular screening alternative to ABI sequencing in the diagnosis of hemoglobinopathies and other genetic disorders in the near future.

Active and break spells of summer monsoon over Bangladesh.

This study examines the intraseasonal variability of the southwest summer monsoon over Bangladesh using rainfall data from rain gauge stations of the Bangladesh Meteorological Department (BMD) collected over 30 years (1988-2017). In this paper, active and break spells are defined as periods during the peak monsoon months of July and August, in which the daily precipitation lasts for three or more days at 0.5 above and below the daily climatological cycle. The active and break phases of the monsoon over a period of 10 years (2008-2017) were also analyzed by The Weather Research and Forecasting (WRF) model simulation for analysing synoptic conditions. The model simulations for each year were done for the period of 1 May to 30 September with 1 May as the initial condition with a single domain of 30 km resolution and 19 vertical levels. The final operational global analysis data from the Global Forecasting System of National Centers for Environment Prediction (NCEP-FNL) with resolution 1° × 1° is used for model simulation. The model-simulated daily rainfall, Sea Level Pressure (SLP), wind pattern at 850 hPa, 200 hPa, and Outgoing Longwave Radiation (OLR) are compared with the observations from Tropical Rainfall Measuring Mission (TRMM), ERA5 (ERA5 is the European Centre for Medium-Range Weather Forecasts fifth-gen global atmospheric reanalysis data), and Kalpana-1. This study also finds that the increase in rainfall is concurrent with a southwesterly wind and the decrease of rainfall simultaneously occurs with a southeasterly wind. Active days were found to have lower OLR values and lower SLP than break days.

Combined effect of Schroth method and Gensingen brace on Cobb's angle and pulmonary functions in adolescent idiopathic scoliosis: a prospective, single blinded randomized controlled trial.

OBJECTIVE: To detect the effect of the Schroth method added to the Gensingen brace for six months on Cobb's angle, axial rotation of the trunk, and pulmonary function in adolescent idiopathic scoliosis. PATIENTS AND METHODS: The study followed a prospective, single-blinded, randomized controlled trial design following the CONSORT guidelines. The study was conducted in Health and Rehabilitation Centre. 42 males aged 10-18 years old, diagnosed with adolescent idiopathic scoliosis (AIS) with curves of 35-40°, were recruited and then were allocated into three groups: Group A, Group B, and Group C. RESULTS: Significant improvement (p < 0.001) in Cobb's angle and the axial rotation of the trunk (ART) were noted after the treatment in the three groups, while pulmonary function showed better results in Group A (p < 0.001) when compared to the two other groups p ≥ 0.000). CONCLUSIONS: Six months of Schroth method added to Gensingen Brace demonstrate favorable results in Cobb's angle, thoracic trunk rotation angle, and pulmonary function in adolescent idiopathic scoliosis.

Retraction Note: Combined effect of Schroth method and Gensingen brace on Cobb's angle and pulmonary functions in adolescent idiopathic scoliosis: a prospective, single blinded randomized controlled trial.

The article "Combined effect of Schroth method and Gensingen brace on Cobb's angle and pulmonary functions in adolescent idiopathic scoliosis: a prospective, single blinded randomized controlled trial", by S.A. Moawd, G. Nambi, A.E. El-Bagalaty, S.M. Hassan, S.E.B. Elsayed, F.M. Aboelmagd, N.A. Alhwoaimel, H.A. Abdeen, published in Eur Rev Med Pharmacol Sci 2023; 27 (2): 601-610-DOI: 10.26355/eurrev_202301_31061-PMID: 36734703 has been retracted by the Editor in Chief for the following reasons: After publication, concerns were raised about the use of copyright and trademark protected terms "Gensingen Brace" and "the Schroth Method" by the authors of the paper without permission for referring to results of a study with treatment of patients with a "Gensingen Brace". The owner of the copyright expressly denies the use of his trademark for scientific statements that are neither authorized by him nor correspond to the product's characteristics and qualities of his well-known products sold under the trademark. After being informed about the copyright and trademark issue, the corresponding author has been acknowledged and affirmed that the authors were not aware of the possible copyright and trademark infringement at the time of submission. The Publisher apologizes for any inconvenience this may cause. https://www.europeanreview.org/article/31061.

Rayleigh-Taylor instability of an ultrathin foil accelerated by the radiation pressure of an intense laser.

We report experimental evidence for a Rayleigh-Taylor-like instability driven by radiation pressure of an ultraintense (10(21) W/cm(2)) laser pulse. The instability is witnessed by the highly modulated profile of the accelerated proton beam produced when the laser irradiates a 5 nm diamondlike carbon (90% C, 10% H) target. Clear anticorrelation between bubblelike modulations of the proton beam and transmitted laser profile further demonstrate the role of the radiation pressure in modulating the foil. Measurements of the modulation wavelength, and of the acceleration from Doppler-broadening of back-reflected light, agree quantitatively with particle-in-cell simulations performed for our experimental parameters and which confirm the existence of this instability.

Carvacrol mitigates vancomycin-induced nephrotoxicity via regulation of IkBα/p38MAPK and Keap1/Nrf2 signaling pathways: an experimental study with in silico evidence.

OBJECTIVE: Despite its evident renal toxicity, vancomycin is considered an effective glycopeptide antibiotic against life-threatening positive bacterial contagions. The current study aimed to investigate the potential protective effects of carvacrol as well as its underlying mechanism against vancomycin-induced nephrotoxicity. MATERIALS AND METHODS: The animals were randomly classified into four groups (8 rats per group). Group I, which served as a control group, received only vehicles. Group II received a single i.p. injection of 50 mg/kg of carvacrol for seven days. Group III received vancomycin (200 mg/kg, i.p.) as a singular daily dose for seven days. Carvacrol was administered to Group IV seven days prior to the daily vancomycin dose. RESULTS: The results revealed that carvacrol minimized vancomycin-induced renal injury as evidenced by lower serum cystatin C levels and kidney injury molecule-1 (KIM-1), in addition to a decline in renal damage caused by vancomycin as indicated in histopathological assessment. Furthermore, carvacrol significantly attenuated oxidative stress parameters and inflammatory mediators. Moreover, it downregulated Keap1, mitogen-activated protein kinase (p38MAPK), and nuclear factor kappa B (NF-κB) genes and proteins, along with controlling the NF-κB inhibitory protein (IkBα) and nuclear factor erythroid 2-related factor 2 (Nrf2) genes and proteins observed through streaming its genes. A molecular docking technique was also used to investigate the potential interactivity between carvacrol and proteins involved in regulating oxidative injury and inflammatory responses. CONCLUSIONS: The current study findings revealed that carvacrol administration before vancomycin could be a promising therapeutic approach for maceration of renal damage stimulated by vancomycin via controlling IkBα/MAPK and Keap1/Nrf2 signaling molecules.https://www.europeanreview.org/wp/wp-content/uploads/graphical-abstract-1.jpg.

Distinct effects of trampoline-based stretch-shortening cycle exercises on muscle strength and postural control in children with Down syndrome: a randomized controlled study.

OBJECTIVE: The aim of the study was to investigate the effect of a 3-month, trampoline-based stretch-shortening cycle (SSC) exercises on muscle strength and postural control in children with Down's syndrome (DS). PATIENTS AND METHODS: Thirty-two children with DS aged between 7-9 years were enrolled and randomly assigned into the control group (n = 16); received standard physical therapy (sPT) or SSC group (n = 16); received sPT in addition to a 15-minute, trampoline-based SSC training program twice per week for 12 successive weeks. Lower limb muscle strength and postural stability [anterior/posterior stability index (A/P-SI), medial/lateral stability index (M/L-SI)], and overall stability index (O-SI) were assessed pre- and post-treatment. RESULTS: Strength of hip extensor (p=0.034) and adductor (p=0.015), knee extensor (p=0.028) and flexor (p=0.01), and ankle dorsi (p=0.033) and plantar flexor (p=0.007) muscles increased significantly in the SSC group when compared with the control group. Also, the A/P-SI (p=0.019), M/L-SI (p=0.002), and O-SI (p=0.021) decreased significantly in the SSC group when compared with the control group, suggesting better postural control. CONCLUSIONS: Twelve weeks of trampoline-based SSC exercises are likely effective for enhancing muscle strength and postural control in children with DS and should consequently be included in the rehabilitation programs for these children.

Infrastructure alone cannot ensure resilience to weather events in drinking water supplies.

Climate resilient water supplies are those that provide access to drinking water that is sustained through seasons and through extreme events, and where good water quality is also sustained. While surface and groundwater quality are widely understood to vary with rainfall, there is a gap in the evidence on the impact of weather and extremes in rainfall and temperature on drinking water quality, and the role of changes in water system management. A three-country (Bangladesh, Nepal and Tanzania) observational field study tracked 2353 households clustered around 685 water sources across seven different geographies over 14 months. Water quality (E. coli) data was modelled using GEE to account for clustering effects and repeated measures at households. All types of infrastructure were vulnerable to changes in weather, with differences varying between geographies; protected boreholes provided the greatest protection at the point of collection (PoC). Water quality at the point of use (PoU) was vulnerable to changes in weather, through changes in PoC water quality as well as changes in management behaviours, such as safe storage, treatment and cleaning. This is the first study to demonstrate the impact of rainfall and temperature extremes on water quality at the PoC, and the role that weather has on PoU water quality via management behaviours. Climate resilience for water supplies needs to consider the infrastructure as well as the management decisions that are taking place at a community and household level.

Lycopene nanoparticles promotes osteoblastogenesis and inhibits adipogenesis of rat bone marrow mesenchymal stem cells.

OBJECTIVE: Lycopene is a carotenoid and antioxidant with potent singlet oxygen quenching ability that reduces oxidative stress and promotes bone health. However, the cellular mechanisms by which lycopene influences bone metabolism are not known. MATERIALS AND METHODS: The present study investigated the effects of lycopene nanoparticles on the differentiation of rat bone marrow-derived mesenchymal stem cells into osteoblasts or adipocytes. RESULTS: In osteogenic medium, lycopene supplementation dose-dependently enhanced osteoblast differentiation, as evidenced by the transcription of Alpl, Runx2, Col1a1, Sp7, and Bglap, higher alkaline phosphatase activity, osteocalcin secretion and extracellular matrix mineralisation seen with Alizarin red S staining, and increased haem oxygenase levels. By contrast, lycopene in adipogenic medium inhibited adipocyte differentiation evidenced by decreases in the transcription of Tnfsf11, Tnfrsf11b, Pparg, Lpl, and Fabp4 and reduced fat accumulation observed by Oil Red O staining. CONCLUSIONS: Lycopene nanoparticles may promote bone health and are considered as a potential candidate for the prevention and/or treatment of bone loss conditions.

Prevalence of ticks (Acari: Ixodidae) and Theileria annulata infection of cattle in Gezira State, Sudan.

This study was conducted in Gezira State, central Sudan during January, May and August 2014 to determine species of ticks infesting cattle and prevalence of Theileria annulata infection and antibodies. A total of 200 head of cattle were sampled for ticks, blood smears, lymph node biopsy smears and sera from five study areas. Zebu (Butana and Kenana) and cross-bred (Zebu X Friesian) cattle of both sexes were sampled. Cattle age groups were younger than one year, one year to younger than four years and four years and older. Coat colour of cattle was recorded. The results revealed that cattle were infested with Rhipicephalus evertsi evertsi (n = 562, 51.6%), Hyalomma anatolicum (n = 289, 26.5%), H. rufipes (n = 70, 6.4%), R. sanguineus sanguineus (n = 59, 5.4%), R. decoloratus (n = 39, 3.6%), Amblyomma lepidum (n = 35, 3.2%), H. impeltatum (n = 26, 2.4%) and H. dromedarii (n = 9, 0.8%). Out of 200 blood and biopsy smears, 33 (16.5%) showed piroplasms for Theileria spp., while 29 (14.5%) were positive for Theileria spp. schizonts. Seropositivity for T. annulata using indirect fluorescent antibody (IFA) test was 94 (47%). It is concluded that there are three genera of hard ticks and eight species in Gezira State and tropical theileriosis is endemic in the state. These findings are the first records in Gezira State, Central Sudan and it is highly recommended to consider the endemicity of tropical theileriosis particularly in cattle upgrading programmes.

Atmospheric factors controlling stable isotope variations in modern precipitation of the tropical region of Bangladesh.

The study investigates the factors that control the isotopic composition of tropical precipitation in Bangladesh. Daily and monthly rainfall samples were collected from three stations from 2013 to 2015: (1) northern and moderately high altitude: Sylhet, (2) middle part of the country (close to Tropic of Cancer): Savar, and (3) southern coastal region: Barisal. To escape from the post-evaporation effect, proper care was adopted. This is supported by the fact that the local meteoric water lines (LMWLs) derived for the daily precipitations of all stations mostly follow the global meteoric water line (GMWL). The results exhibit a clear seasonal and spatial variation in both δ 18O and δ 2H of precipitation. 18O is more depleted in samples collected during rainy (monsoon) seasons, while 18O enrichment is associated to winter and summer (pre-monsoon) seasons. During rainy season, intra-seasonal variability of δ18O rainfall is clearly seen and remarkable depletion of 18O is observed during the period of intense convective activity over the Bay of Bengal. This feature indicates that isotope variability in Bangladesh is controlled by large-scale convective activity rather than local rainfall amount.

Systematics of early and middle Miocene Old World monkeys.

New information about the early cercopithecoids Prohylobates tandyi (Wadi Moghra, Egypt) and Prohylobates sp. indet. (Buluk and Nabwal, Kenya) is presented. Comparisons are made among all major collections of Early and Middle Miocene catarrhine monkeys, and a systematic revision of the early Old World monkeys is provided. Previous work involving the systematics of early Old World monkeys (Victoriapithecidae; Cercopithecoidea) has been hampered by a number of factors, including the poor preservation of Prohylobates material from North Africa and lack of comparable anatomical parts across collections. However, it is now shown that basal cercopithecoid species from both northern and eastern Africa can be distinguished from one another on the basis of degree of lower molar bilophodonty, relative lower molar size, occlusal details, symphyseal construction, and mandibular shape. Results of particular interest include: 1) the first identification of features that unambiguously define Prohylobates relative to Victoriapithecus; 2) confirmation that P. tandyi is incompletely bilophodont; and 3) recognition of additional victoriapithecid species.

Ticks (Acari: Ixodidae) infesting camels (Camelus dromedarius) in Northern Sudan.

Monthly total body tick collections from 13-20 camels were conducted for 2 consecutive years (2000-2001) in Northern Sudan. Tick populations were correlated with locality, season, predeliction site, sex and coat colour. Hyalomma dromedarii was found to be the predominant (89%) tick species infesting the camels. Other tick species found in very low numbers were Hyalomma impeltatum (7.7%), Hyalomma anatolicum anatolicum (3.3%), Hyalomma truncatum (0.29%), Hyalomma marginatum rufipes (0.25%), Rhipicephalus praetextatus (0.30%) and Rhipicephalus sanguineus group (0.09%). Nymphs of the genus Hyalomma were collected in significant numbers. Adult ticks significantly preferred to attach to the lower parts of the camel's body for feeding while the nymphs preferred the back of the animal. Female camels harboured more ticks than males while higher infestations were recorded on camels with a grey coat colour compared to those with a brown coat colour. Ticks were found on camels throughout the year and increased in numbers during March to October with a peak in September.

Nano-sized mesoporous phosphated tin oxide as an efficient solid acid catalyst.

Herein, we prepared a mesoporous tin oxide catalyst (mSnO2) activated with phosphate species by the adsorption of phosphate ions from a phosphoric acid solution onto tin oxyhydroxide (Sn(OH)4) surface. The phosphate content ranged from 3 to 45 wt%. The nonaqueous titration of n-butylamine in acetonitrile was used to determine the total surface acidity level. FTIR of chemically adsorbed pyridine was used to differentiate between the Lewis and Brönsted acid sites. Thermal and X-ray diffraction analysis indicated that the addition of phosphate groups stabilized the mesostructure of mSnO2 and enabled it to keep its crystalline size at the nanoscale. FTIR analysis indicated the polymerization of the HPO4 2- groups into P2O7 4-, which in turn reacts with SnO2 to form a SnP2O7 layer, which stabilizes the mesoporous structure of SnO2. The acidity measurements showed that the phosphate species are distributed homogeneously over the mSnO2 surface until surface saturation coverage at 25 wt% PO4 3-, at which point the acid strength and surface acidity level are maximized. The catalytic activity was tested for the synthesis of hydroquinone diacetate, where it was found that the % yield of hydroquinone diacetate compound increased gradually with the increase in PO4 3- loading on mSnO2 until it reached a maximum value of 93.2% for the 25% PO4 3-/mSnO2 catalyst with 100% selectivity and excellent reusability for three consecutive runs with no loss in activity.

Assessment of the prevalence of Theileria lestoquardi in sheep from the Sudan using serological and molecular methods.

Malignant theileriosis of sheep and goats caused by Theileria lestoquardi is considered to be among the most important tick borne diseases in the Sudan. Information on the prevalence of the disease in different parts of the Sudan is limited. The purpose of this study was to estimate the prevalence of the disease in five states of the Sudan using molecular and serological assays. A total of 393 blood and serum samples from clinically asymptomatic sheep were analysed using nested reverse line blot (nRLB) and loop mediated isothermal amplification (LAMP), as well as an enzyme-linked immunosorbent assay (ELISA). The results indicated a sero-prevalence of 33.8% while RLB and LAMP assays revealed molecular prevalences of 29.5 and 22.6% respectively. The prevalence of Theileria lestoquardi varied significantly according to the geographical origin of the infected animals, whereas age and gender did not have a significant effect. RLB data indicated that T. lestoquardi usually occurred as a co-infection with the non-pathogenic Theileria ovis. Using RLB as a gold standard, a sensitivity of 68.1% and a specificity of 96.4% were recorded for LAMP and a sensitivity of 75.9% and a specificity of 83.8% for ELISA. The Kappa coefficient between nRLB and LAMP indicated a significant level of agreement (0.692), but only moderate concordance (0.572) between nRLB and ELISA. The results of the present study confirm and extend earlier findings regarding the widespread of T. lestoquardi infections in sheep in the Sudan. The data provide evidence that should enable the veterinary authorities to deploy appropriate control measures.

Reliminary survey of ticks (Acari: Ixodidae) on cattle in Central Equatoria State, Southern Sudan.

In a preliminary survey conducted in 2005, the species composition and seasonality of ticks infesting cattle in Central Equatoria State, Southern Sudan was determined. Three locations were selected (Gumbo, Khor Rumla and Nyaing) and surveyed every 3 months. Two cattle herds in each of the three locations were visited four times during the study period. Total body collections of ticks were made from each of five cattle (Nilotic Zebu breed) kept in six different herds. Four tick genera and ten species were identified. The tick species identified were Amblyomma lepidum, Amblyomma variegatum, Boophilus annulatus, Boophilus decoloratus, Hyalomma marginatum rufipes, Hyalomma truncatum, Rhipicephalus appendiculatus, Rhipicephalus evertsi evertsi, Rhipicephalus praetextatus and Rhipicephalus sanguineus group. The highest number of ticks was collected in October during the rainy season. A finding of great significance was that R. appendiculatus, vector of East Coast fever, has now firmly established itself throughout the year with possible implications for cattle production in Central Equatoria State.

Molecular, serological and parasitological survey of Theileria annulata in North Kordofan State, Sudan.

This survey was conducted to detect Theileria annulata infection in indigenous (Zebu) and cross (indigenous × Friesian) cattle (n 604) of different sex & age groups, and to investigate prevalence of ticks infesting cattle in North Kordofan State, Sudan. Ticks, blood smears, serum samples and blood spots on filter paper were collected from four municipalities at four monthly intervals during the period from August 2008 to July 2009. Out of 604 blood smears, 69 (11.4%, 95% CI: 16.9-5.9) were positive for Theileria piroplasms; whereas, out of 240 serum samples, 164 (68.3%, 95% CI: 89.3-47.3) were positive for antibodies against T. annulata using IFA test. Using PCR, out of 100 blood spots on filter paper, 8 (8%, 95% CI: 9.6-6.4) revealed T. annulata DNA. The highest prevalence of Theileria spp. piroplasms and T. annulata antibodies was recorded in August (15.9 ±â€¯4.6) followed by February (13.3 ±â€¯3.8) and the lowest was in May (3.9 ±â€¯1.0). Among cattle breeds, the highest prevalence of piroplasms (11.9 ±â€¯5.0) and T. annulata antibodies (68.9 ±â€¯10) was among zebu cattle. PCR assays revealed the highest prevalence rate of T. annulata DNA in February (12.5 ±â€¯1.4) followed by August (6.1 ±â€¯0.7) and the lowest was in May (5.7 ±â€¯0.7). According to cattle breeds, T. annulata DNA was detected in cross-bred cattle (3.3 ±â€¯0.4) more than zebu cattle (10 ±â€¯1.6). Seven tick species belonging to four genera and were identified, Amblyomma lepidum, Hyalomma dromedarii, H. impeltatum, H. rufipes, Rhipicephalus evertsi evertsi, Rhipicephalus decoloratus, R. annulatus. The most abundant tick species was H. rufipes followed by H. impeltatum, R. e. evertsi, H. dromedarii, R. decoloratus, A. lepidum and R. annulatus. Theileria annulata has been detected for the first time in North Kordofan State, though at a low prevalence rate. This finding is an alarming situation since tropical theileriosis is an emerging disease and it is widely believed that this economically important tick-borne parasite does not exist in this state. The possible alternative vector(s) of T. annulata in the absence of the known vector H. anatolicum is discussed.

Comparisons among two serological tests and microscopic examination for the detection of Theileria annulata in cattle in northern Sudan.

We tested the agreement between microscopic examination (ME), a surface protein-detecting enzyme-linked immunosorbent assay (TaSP ELISA) and an indirect fluorescent assay (IFA) for detection of Theileria annulata in 2,661 naturally infected cattle from northern Sudan (samples collected between June 2001 and July 2002). In the ME, we detected piroplasms in 364/2,661 cattle (14%), and the kappas between the ME and the serological tests were poor (TaSP ELISA 10%; IFA 8%). The TaSP ELISA detected 885/2,661 cattle as positive, and the Rogan-and-Gladen corrected true prevalence of this sample was estimated to be 30%. The relative sensitivity and specificity of the IFA (compared to the previously validated TaSP ELISA) were 70.7% and 81.8%, respectively.

Distribution and population dynamics of ticks (Acari: Ixodidae) infesting sheep in Sennar State, Sudan.

A cross-sectional survey of ticks infesting sheep was conducted in Sennar State, Sudan. A total body collection of ticks was carried out at five localities Sennar town, Singa, Dinder, Abu Naama and Um Banein on two types of Desert sheep (Watish and Ashgar) on two farms at each locality at two monthly intervals for one year starting July 2002 to May 2003. Four tick genera and eight species were identified. They were Amblyomma lepidum, Hyalomma anatolicum anatolicum, Hyalomma truncatum, Rhipicephalus (Boophilus) decoloratus, Rhipicephalus camicasi, Rhipicephalus evertsi evertsi, Rhipicephalus guilhoni and Rhipicephalus muhsamae. A significant (P < or = 0.05) seasonal pattern of activity was observed for A. lepidum and R. guilhoni with peak activity occurring during rainy seasons. The highest mean number of ticks (7.26 +/- 0.58) was recorded at Abu Naama, while the lowest mean (3.61 +/- 0.31) was recorded in Sennar. Watish type sheep carried significantly (P < or = 0.05) more ticks than Ashgar type.

Aflatoxin biosynthesis control produced by Aspergillus flavus in layer hens feed during storage period of six months.

Aflatoxins (AFTs) are a group of closely related toxins that are produced by different fungus species. Food and feed contamination with AFT is a worldwide health-related problem. As a result of fungal attack, the food and feed resulted in a principal socioeconomic loss and toxins produced in feed and food items harm the humans and animals in different ways. The anti-aflatoxigenic effect Psidium guajava, Ficus benghalensis, Gardenia radicans, Punica granatum and Ziziphus jujuba leaves were evaluated against aflatoxins (AFTs), produced by Aspergillus flavus in layer feed during storage. Among the investigated medicinal plant leaves, P. granatum showed highly promising anti-aflatoxigenic activity and completely inhibited the AFTs (B1 and B2) production over storage period without compromising the nutritive quality of feed (ash, protein, fat, fiber, Fe, Ca, P and K contents). Leaves of F. benghalensis and Z. jujuba were also effective however, higher concentration (15%) inhibited the AFTs production up to 99% and also maintained nutritive quality of feed. G. radicans was found least effective in controlling the AFTs production. Results revealed that all plant leaves were effective in controlling AFTs production in layer feed over the storage period of six months and these plants are potential candidate to replace the fungicides used to protect feed and other agricultural commodities from AFTs production during storage.