RESUMO
In this study, serological screening for Toxoplasma gondii, Trichinella spp., and SARS-CoV-2 in domestic cats was conducted, aiming to identify their exposure to the mentioned pathogens and to assess the risk of potential human infection. In total, serum samples from 481 (310 owned and 171 shelter cats) were collected in Bratislava from September 2020 to September 2021, a period that included the initial outbreak wave of the COVID-19 pandemic. The study showed a 37.4% (135/441) seroprevalence of T. gondii with a slightly lower seropositivity in shelter cats (35.9%; 61/170) than in owned cats (38.4%; 104/271), but this difference was not statistically significant. Overall, the seroprevalence of Trichinella spp. was 2.0% (9/441), with animals from shelters being positive but not significantly more often (2.9%; 5/170) than owned cats (1.5%; 4/271). SARS-CoV-2 antibodies were detected in 2.7% (13/481) of cat sera (2.9% in shelter cats; 2.6% in owned cats). Among ten samples positive by virus neutralisation assay, two were positive for the B.1 variant. The presence of the SARS-CoV-2 virus in buccal and rectal swabs (n = 239) was not detected. The seroprevalence of almost 40% for T. gondii in cats suggests a non-negligible risk of human infection. The study confirmed the possibility of Trichinella spp. infection in cats, and thus the possibility of infection spreading between the sylvatic and synanthropic cycle via this animal species. The presented results also showed that the SARS-CoV-2 virus is likely to circulate in cat populations in Slovakia, not only in cats that may have been in contact with infected persons, but also in shelter cats.
RESUMO
Ticks are important vectors of pathogens affecting humans and animals worldwide. They do not only carry pathogens but diverse commensal and symbiotic microorganisms are also present in ticks. A molecular screening for tick-borne pathogens and endosymbionts was carried out in Ixodes ricinus, Dermacentor reticulatus and Haemaphysalis inermis questing ticks collected in Slovakia. The presence of Rickettsia spp., Coxiella burnetii, Coxiella-like and Francisella-like microorganisms was evaluated by PCR in 605 individuals and by randomly sequencing 66 samples. Four species of rickettsiae (R. raoultii, R. slovaca, R. helvetica and R. monacensis) were identified and reported with an overall prevalence range between 0.4 and 50.3% (±8.0) depending on tick species, sex and locality. Partial sequencing of the gltA gene of 5 chosen samples in H. inermis showed 99% identity with Candidatus Rickettsia hungarica. The total prevalence of C. burnetii in ticks was 2.2⯱â¯1.7%; bacteria were confirmed in I. ricinus and D. reticulatus ticks. The sequences from 2 D. reticulatus males and 1 I. ricinus female ticks were compared to GenBank submissions and a 99.8% match was obtained with the pathogenic C. burnetii. Coxiella-like endosymbionts were registered in all three species of ticks from all studied sites with an average prevalence of 32.7⯱â¯3.7%. A phylogenetic analysis of this Coxiella sp. showed that it does not group with the pathogenic C. burnetii. The prevalence of Francisella-like microorganisms in questing ticks was 47.9⯱â¯3.9%, however H. inermis (nâ¯=â¯108) were not infested. Obtained sequences were 98% identical with previously identified Francisella-like endosymbionts in D. reticulatus and I. ricinus. Coxiella-like and Francisella-like microorganisms were identified for the first time in Slovakia, they might be considered as a non-pathogenic endosymbiont of I. ricinus, D. reticulatus and H. inermis, and future investigations could aim to assess their role in these ticks. However, this work provided further data and broadened our knowledge on bacterial pathogens and endosymbionts present in ticks in Slovakia to help understanding co-infestations, combined treatments and public health issues linked to tick bites.