Mycorrhizal diversity and community composition in co-occurring Cypripedium species.

Orchids commonly rely on mycorrhizal fungi to obtain the necessary resources for seed germination and growth. Whereas most photosynthetic orchids typically associate with so-called rhizoctonia fungi to complete their life cycle, there is increasing evidence that other fungi may be involved as well and that the mycorrhizal communities associated with orchids may be more diverse. Coexisting orchid species also tend to associate with different fungi to reduce competition for similar resources and to increase long-term population viability. However, few studies have related the mycorrhizal communities in the rhizosphere to communities found in the roots of closely related coexisting orchid species. In this study, we used high-throughput sequencing to investigate the diversity and community composition of orchid mycorrhizal fungi in the roots and the rhizosphere of four Cypripedium species growing in forests in Northeast China. The results showed that the investigated Cypripedium species associated with a wide variety of fungi including members of Tulasnellaceae, Psathyrellaceae, and Herpotrichiellaceae, whereas members of Russulaceae, Cortinariaceae, Thelephoraceae, and Herpotrichiellaceae showed high abundance in rhizosphere soils. The diversity of fungi detected in the rhizosphere soil was much higher than that in the roots. The observed variation in fungal communities in Cypripedium roots was not related to forest site or orchid species. On the other hand, variation in mycorrhizal communities of rhizosphere soil was significantly related to sampling site. These results indicate that orchid mycorrhizal communities in the rhizosphere display considerable variation among sites and that orchids use only a subset of the locally available fungi. Future studies focusing on the fine-scale spatial distribution of orchid mycorrhizal fungi and more detailed assessments of local environmental conditions will provide novel insights into the mechanisms explaining variation of fungal communities in both orchid roots and the rhizosphere.

Assessing outdoor air quality vertically in an urban street canyon and its response to microclimatic factors.

The vertical distribution of air pollutants in urban street canyons is closely related to residents' health. However, the vertical air quality in urban street canyons has rarely been assessed using field observations obtained throughout the year. Therefore, this study investigated the seasonal and annual concentrations of particulate matter (PM2.5 and PM10), CO, NO2, SO2, O3, air quality index, and their responses to microclimatic factors at three height levels (1.5, 27, and 69 m above street level) in an urban street canyon. The PM concentration was higher at 27 m than at 1.5 m in winter, whereas the situation was reversed in other seasons. It was found that photochemical pollutants such as NO2 and O3 were the primary pollutants in the urban street canyon. The days on which O3 was the primary pollutant at the height of 1.5 m accounted for 81.07% of the entire year. The days on which NO2 was the primary pollutant at the height of 27 and 69 m accounted for 82.49% and 72.33% of the entire year, respectively. Substantially higher concentrations of NO2 and O3 were found at the height of 27 m than at 69 m. In-canyon concentrations of NO2 and O3 were strongly correlated with air temperature, wind speed, and wind direction, which played important roles in photochemical reactions and pollutant dispersion.

Responses of Growth, Oxidative Injury and Chloroplast Ultrastructure in Leaves of Lolium perenne and Festuca arundinacea to Elevated O3 Concentrations.

The effects of increasing atmospheric ozone (O3) concentrations on cool-season plant species have been well studied, but little is known about the physiological responses of cool-season turfgrass species such as Lolium perenne and Festuca arundinacea exposed to short-term acute pollution with elevated O3 concentrations (80 ppb and 160 ppb, 9 h d−1) for 14 days, which are widely planted in urban areas of Northern China. The current study aimed to investigate and compare O3 sensitivity and differential changes in growth, oxidative injury, antioxidative enzyme activities, and chloroplast ultrastructure between the two turf-type plant species. The results showed that O3 decreased significantly biomass regardless of plant species. Under 160 ppb O3, total biomass of L. perenne and F. arundinacea significantly decreased by 55.3% and 47.8% (p < 0.05), respectively. No significant changes were found in visible injury and photosynthetic pigment contents in leaves of the two grass species exposed to 80 ppb O3, except for 160 ppb O3. However, both 80 ppb and 160 ppb O3 exposure induced heavily oxidative stress by high accumulation of malondialdehyde and reactive oxygen species in leaves and damage in chloroplast ultrastructure regardless of plant species. Elevated O3 concentration (80 ppb) increased significantly the activities of superoxide dismutase, catalase and peroxidaseby 77.8%, 1.14-foil and 34.3% in L. perenne leaves, and 19.2%, 78.4% and 1.72-fold in F. arundinacea leaves, respectively. These results showed that F. arundinacea showed higher O3 tolerance than L. perenne. The damage extent by elevated O3 concentrations could be underestimated only by evaluating foliar injury or chlorophyll content without considering the internal physiological changes, especially in chloroplast ultrastructure and ROS accumulation.

Soil high Cd exacerbates the adverse impact of elevated O3 on Populus alba 'Berolinensis' L.

Pollution with both heavy metal and ground-level ozone (O3) has been steadily increasing, especially in the cities with heavy industry. Little information is known about their combined impacts on urban tree. This study was aimed at characterizing the interactive effects of soil cadmium (Cd) addition and O3 fumigation on visible injury and growth, photosynthesis, oxidative stress, antioxidant enzyme activities, abscisic acid (ABA) content and bioaccumulation of Cd in one-year-old Populus alba 'Berolinensis' saplings by using open top chambers in Shenyang city with developed heavy industry, Northeast China. In this study, poplar saplings were grown in the pots containing soil with different concentrations of Cd (0, 100 and 500 mg kg-1) under ambient air (40 µg L-1) and elevated O3 (120 µg L-1). The results showed that EO and its combination with high Cd (500 mg kg-1) induced significant foliar injury symptoms, decreased root weight (by 41.6%) and total biomass (by 17.4%), inhibited net photosynthetic rate and stomatal conductance, and increased malondialdehyde and ABA contents after 4 weeks of O3 exposure. Elevated O3 exacerbated the accumulation of Cd in leaves and stems of poplar plants grown in the pots with high Cd-polluted soil. Our results also indicated that high Cd pollution in soil increased the susceptibility of plants to O3 and exacerbated the adverse impact of elevated O3 on physiological metabolisms of poplar species, which implied that it was very necessary to take into consideration for O3-tolerance of tree species during phytoremediation of Cd-polluted soil in heavy industrial areas.

Efficiency of nitrogen and phosphorus removal by six macrophytes from eutrophic water.

Increased nitrogen and phosphorus pollution causes eutrophication in water bodies. Using aquatic plants to remove nutrients from water is an attractive phytoremediation. It is a cost-effective, environment-friendly, and efficient way that reduces water body eutrophication by the plant. It is important to choose suitable macrophytes to remove excess N and P under different nutrient conditions. In this study, six macrophyte species (Polygonum orientale, Juncus effuses, Iris pseudocorus, Phragmites australis, Iris sanguinea, Typha orientalis) were tested. Simulation experiment was conducted under five N and P levels. The removal rate, relative growth rate, and the dynamic nutrition concentration of cultivated solution were investigated. Of all the treatment, a 23-95% reduction in N removal and a 29-92% reduction in P removal were recorded. The results showed I. sanguinea is a promising species to treat various eutrophic waters and the other five species can be used specifically to treat certain types of water. The data provided a theoretical guidance to plant species selection for phytoremediation of polluted water bodies for the purpose of water quality improvement around the different reservoir in northern China.

Ethylenediurea (EDU) pretreatment alleviated the adverse effects of elevated O3 on Populus alba "Berolinensis" in an urban area.

Ethylenediurea (EDU) has been used as a chemical protectant against ozone (O3). However, its protective effect and physiological mechanisms are still uncertain. The present study aimed to investigate the changes of foliar visible injury, physiological characteristics and emission rates of volatile organic compounds (VOCs) in one-year-old Populus alba "Berolinensis" saplings pretreated with EDU and exposed to elevated O3 (EO, 120 µg/m3). The results showed that foliar visible injury symptoms under EO were significantly alleviated in plants with EDU application (p < 0.05). Under EO, net photosynthetic rate, the maximum photochemical efficiency of PSII and the photochemical efficiency of PSII of plants pretreated with 300 and 600 mg/L EDU were similar to unexposed controls and significantly higher compared to EO-stressed plants without EDU pretreatment, respectively. Malondialdehyde content was highest in EO without EDU and decreased significantly by 14.9% and 21.3% with 300 and 600 mg/L EDU pretreatment, respectively. EDU pretreatment alone increased superoxide dismutase activity by 10-fold in unexposed plants with further increases of 88.4% and 37.5% in EO plants pretreated with 300 and 600 mg/L EDU pretreatment, respectively (p < 0.05). Abscisic acid content declined under EO relative to unexposed controls with the effect partially reversed by EDU pretreatments. Similarly, VOCs emission rate declined under EO relative to unexposed plants with a recovery of emission rate observed with 300 and 600 mg/L EDU pretreatment. These findings provided significant evidence that EDU exerted a beneficial effect and protection on the tested plants against O3 stress.

Evaluation of hyperaccumulation potentials to cadmium (Cd) in six ornamental species (compositae).

Phytoremediation is considered as a promising soil remediation technique. In the present study, the growth responses, cadmium (Cd) accumulation and uptake capability of six popular compositae species, namely, Taraxacum mongolicum Hand.-Mazz., Tagetes erecta L., Tagetes patula L., Zinnia elegans Jacq., Centaurea cyanus L. and Gerbera jamesonii Bolus under Cd stress were investigated. Among the six compositae species, the growth of T. erecta L. and T. patula L. improved under 10 mg kg-1 Cd exposure in term of the total biomass and height increased along with the increased Cd concentration in soil, and the growth of the two plants had no significant differences at the high Cd concentration (100 mg kg-1), which indicated that they have good tolerance to Cd toxicity. At the same time, the two plants have higher biomass than four other plants. Furthermore, they can accumulate Cd above 100 µg g-1 dry tissue, which is the threshold value of a Cd-hyperaccumulator, and have higher Cd uptake ability, translocation factor (TF) and bioconcentration factor (BCF) values. According to these traits, it was shown that T. erecta L. and T. patula L. had strong tolerance and accumulation capability to Cd, therefore they can become potential hyperaccumulators in phytoremediation of Cd-contaminated soils.

Glomalin changes in urban-rural gradients and their possible associations with forest characteristics and soil properties in Harbin City, Northeastern China.

Glomalin-related soil protein (GRSP) is a glycoprotein from the hyphae and spores of arbuscular mycorrhizal fungi. Despite urbanization being the leading cause of present-day land-use changes, there is limited information available on the effects of urbanization on GRSP. We sampled soil from 257 plots in Harbin City, China, and surveyed forest characteristics, soil properties, and urbanization gradients related to ring road development, urban history, and land use. Two glomalin components (easily extracted glomalin, EEG; and total glomalin, TG) and their relative contributions to soil organic carbon (SOC: EEG/SOC, TG/SOC) were measured in the laboratory. We found exponential increases in EEG/SOC and TG/SOC from the most urbanized to the most rural regions, indicating that urbanization sharply reduced glomalin-related SOC sequestration. In general, 1.3-1.4-fold higher glomalin levels were found in the newly urbanized, previously rural areas, while glomalin contribution to SOC sequestration was lower by 38-59% for EEG and 74-85% for TG in the most urbanized regions compared to rural regions. Accompanying these recorded changes in glomalin, linear decreases in soil pH and electrical conductance were observed in all three urban-rural gradients from the urban center to the rural area, and steep decreases in conifer ratio and shrub richness were seen in two of the gradients. The complex associations among glomalin and forest characteristics, soil properties, and urbanization gradients were decoupled and cross-checked using redundancy analysis variation partitioning and structural equation model analysis. Urbanization indirectly changed glomalin features by altering soil properties, with soil properties accounting for over 60% of the glomalin variation. Forest characteristics and urbanization gradients contributed to 10-15% of the glomalin variation. With rapid urbanization occurring in China and on a global scale, glomalin variation should be considered when evaluating soil carbon sequestration and in developing effective forest management strategies, with the aim of ameliorating soil degradation in urbanized regions by rehabilitating glomalin accumulation.

Drought Alleviated the Negative Effects of Elevated O3 on Lonicera maackii in Urban Area.

Open top chambers were used to study the changes in photosynthesis, physiology and stomata characteristics in 1-year-old Lonicera maackii seedlings exposed to drought (DT, 30%-35% soil saturated water content) or/and elevated ozone (EO, 80 ppb). The results showed that DT or/and EO significantly decreased net photosynthetic rate (Pn), stomatal conductance (gs), maximum photochemical efficiency (Fv/Fm), but increased the activity of superoxide dismutase (SOD), and malondialdehyde content (p < 0.05). Compared with EO alone, the combination of EO and DT caused higher values in Pn, Fv/Fm, SOD activity (p < 0.05), and smaller stomata size and lower visible injury rate. DT alleviated the adverse impact of EO on the shrub by increasing enzyme activity and decreasing stomatal size, particularly stomatal width. The study provided increasing evidence that moderate drought might exert a beneficial effect on the tested plants to adapt to the future climate change, particularly in high ozone regions.

Regulatory Effects of Elevated Carbon Dioxide on Growth and Biochemical Responses to Ozone Stress in Chinese Pine (Pinus tabulaeformis Carr.).

This study examined whether carbon dioxide (CO2) might alleviate ozone (O3) injury to the dominant coniferous forest species of northern China, Pinus tabulaeformis Carr. After 90 days O3 exposure, biomass and net photosynthetic rate (Pn) decreased significantly by 24.44 % and 42.89 % compared with the control. A significant increase in malondialdehyde (MDA) was shown, suggesting cell membrane damage and oxidative stress. However, the positive responses of biomass dry weight, antioxidative enzymes and soluble sugar contents under elevated CO2 alone and the combination of elevated CO2 and O3 were observed, indicating that CO2 could ameliorate O3-induced injury. The study provided increasing evidence that moderately elevated CO2 levels may have a beneficial effect on the forest ecosystem to respond to global climate change.

Coordinated regulation of arbuscular mycorrhizal fungi and soybean MAPK pathway genes improved mycorrhizal soybean drought tolerance.

Mitogen-activated protein kinase (MAPK) cascades play important roles in the stress response in both plants and microorganisms. The mycorrhizal symbiosis established between arbuscular mycorrhizal fungi (AMF) and plants can enhance plant drought tolerance, which might be closely related to the fungal MAPK response and the molecular dialogue between fungal and soybean MAPK cascades. To verify the above hypothesis, germinal Glomus intraradices (syn. Rhizophagus irregularis) spores and potted experiments were conducted. The results showed that AMF GiMAPKs with high homology with MAPKs from Saccharomyces cerevisiae had different gene expression patterns under different conditions (nitrogen starvation, abscisic acid treatment, and drought). Drought stress upregulated the levels of fungi and soybean MAPK transcripts in mycorrhizal soybean roots, indicating the possibility of a molecular dialogue between the two symbiotic sides of symbiosis and suggesting that they might cooperate to regulate the mycorrhizal soybean drought-stress response. Meanwhile, the changes in hydrogen peroxide, soluble sugar, and proline levels in mycorrhizal soybean as well as in the accelerated exchange of carbon and nitrogen in the symbionts were contributable to drought adaptation of the host plants. Thus, it can be preliminarily inferred that the interactions of MAPK signals on both sides, symbiotic fungus and plant, might regulate the response of symbiosis and, thus, improve the resistance of mycorrhizal soybean to drought stress.

Elevated CO2 ameliorated the adverse effect of elevated O3 in previous-year and current-year needles of Pinus tabulaeformis in urban area.

The effects of elevated CO2 (700 ppm) and/or elevated O3 (80 ppb) on different-age needles of Pinus tabulaeformis were studied in open top chambers. Elevated CO2 increased needle dry weight, net photosynthetic rate, and maximum quantum yield of photosystem II in previous and current-year needles. Elevated O3 significantly decreased dry weight, net photosynthetic rate, and maximum quantum yield of photosystem II only in previous-year needles. Elevated O3 increased hydrogen and malondialdehyde content in previous-year needles, but did not significantly increase hydrogen peroxide content in current-year needles, which indicated that oxidative stress induced by elevated O3 was more severe in previous-year needles than in current-year needles. Our results showed that the adverse effect of elevated O3 in needles of P. tabulaeformis was ameliorated by elevated CO2.

Spatiotemporal patterns of urban forest carbon sequestration capacity: Implications for urban CO2 emission mitigation during China's rapid urbanization.

Urban forests provide ecological functions and human well-being. However, spatiotemporal changes in urban forest carbon sequestration (CS) under rapid urbanization remain poorly understood. We established a model to predict the annual CS dynamics in urban forests based on plot-measured CS and Landsat images. Our results showed that the urban forest coverage in Changchun increased from 18.09 % to 24.01 % between 2000 and 2019, especially in the urban suburbs. However, urban forest patches became more fragmented and less connected, particularly in the urban center. The NDVI is better than other vegetation indices for mapping urban forest CS. We observed a gradual increase in urban forest CS capacity from 2000 to 2019, with higher CS capacity found in urban suburbs compared to urban centers. The class distribution of urban forest CS capacity was skewed toward low values (0-2 g·m-2·d-1), but this tendency diminished gradually. In 2000, the urban forest in Changchun offset approximately 2.11 % of carbon emissions but declined to 0.88 % by 2019 due to increased carbon emissions. Rapid urbanization was the main factor affecting CS, with impervious surface area accounting for 48.7 % of the variation. Urban landscape pattern indices also influenced the CS, with higher forest patch connectivity and lower patch density leading to greater CS capacity. Our study helps urban managers develop urban greening strategies for carbon neutrality and low-carbon city.

Effects of geographical, soil and climatic factors on the two marker secondary metabolites contents in the roots of Rubia cordifolia L.

The growth and quality of medicinal plants depend heavily on environmental variables. The quality of Rubia cordifolia, an important medicinal plant, is determined by the two main secondary metabolites of the root, purpurin and mollugin. However, their relationship with environmental factors has not been studied. In this study, the purpurin and mollugin contents of R. cordifolia roots from different sampling sites in China were measured using ultra-high-performance liquid chromatography, and the correlations between the two secondary metabolites and environmental variables were analyzed. The results showed that there were significant differences in the contents of purpurin and mollugin in the roots of R. cordifolia at different sampling points. The content of purpurin ranged from 0.00 to 3.03 mg g-1, while the content of mollugin ranged from 0.03 to 10.09 mg g-1. The quality of R. cordifolia in Shanxi, Shaanxi and Henan border areas and southeastern Liaoning was higher. Liaoning is expected to become a R. cordifolia planting area in Northeast China. Correlation and regression analysis revealed that the two secondary metabolites were affected by different environmental factors, the two secondary metabolites contents were positively correlated with longitude and latitude, and negatively correlated with soil nutrients. In addition, higher temperature and shorter sunshine duration facilitated the synthesis of purpurin. Annual precipitation might be the main factor limiting the quality of R. cordifolia because it had opposite effects on the synthesis of two major secondary metabolites. Therefore, this study is of great significance for the selection of R. cordifolia planting areas and the improvement of field planting quality.

Hormesis phenomena under Cd stress in a hyperaccumulator--Lonicera japonica Thunb.

A hydroponic experiment was carried out to investigate possible hormetic response induced by cadmium (Cd) in a potential hyperaccumulator-Lonicera japonica Thunb. The results showed that Cd at low concentrations induced a significant increase in plant growth, leaf water content and content of photosynthetic pigments in L. japonica, but decreased them at high concentrations, displayed inverted U-shaped dose response curves, confirming a typical biphasic hormetic response. The U-shaped dose response curves were displayed in malondialdehyde (MDA) and electrolyte leakage in leaves at low doses of Cd, indicating reduce oxidative stress and toxic effect. The increase of superoxide dismutase (SOD) and catalase (CAT) activities was observed along with the increased Cd concentration, indicative of increase in anti-oxidative capacity that ensures redox homeostasis is maintained. After 28 days exposure to 10 mg L(-1) Cd, stem and leaf Cd concentrations reached 502.96 ± 28.90 and 103.22 ± 5.62 mg kg(-1) DW, respectively and the plant had high bioaccumulation coefficient (BC) and translocation factor (TF'). Moreover, the maximum TF value was found at 2.5 mg L(-1) Cd treatment, implying that low Cd treatment improved the ability to transfer Cd from medium via roots to aerial structures. Taking together, L. japonica could be considered as a new plant to investigate the underlying mechanisms of hormesis and Cd tolerance. Our results suggest that hormetic effects should be taken into consideration in phytoremediation of Cd-contaminated soil.

Energy financing, energy projects retrofit and energy poverty: a scenario-analysis approach for energy project cost estimation and energy price determination.

The research aims to determine the nexus of energy projects retrofit and poverty under two scenarios: energy project cost estimation and energy price determination. Households in rural areas of northern China are now required to switch from coal to cleaner heating options, including natural gas and electricity, as part of a government-led clean heating initiative. This initiative significantly increased the heating expense for participating homes, even when substantial subsidies were applied. We surveyed a large number of northern Chinese households to learn more about the rise in energy insecurity that has been attributed to government action. Our research shows that switching to electricity and gas from coal considerably worsens energy poverty in several ways, whereas switching to clean coal improves the situation. According to an econometric study, changes in energy poverty reveal heterogeneity in several ways. There is little change in Beijing, while the considerably less developed province of Hebei to the north sees a 75% rise. Energy poverty is more common in families with poorer incomes, lower levels of education, and smaller sizes. People who lack resources to insulate their homes will feel the effects more acutely. These results support the idea that low-income families would suffer disproportionately under a "one policy for all" mandate. For policymakers working on energy transition strategies for a low-carbon economy, it highlights the need to consider the distributional impact.

Changes in marker secondary metabolites revealed the medicinal parts, harvest time, and possible synthetic sites of Rubia cordifolia L.

Rubia cordifolia L. is a significant medicinal plant. To investigate the changes of marker metabolites of R. cordifolia, the purpurin, mollugin, carbon, nitrogen contents, and the expression of genes involved in anthraquinones synthesis were examined. The findings indicated that the two secondary metabolites were only detected in stems and roots. Root purpurin content was 5-26 times higher than in stems, and root mollugin content was 92 times higher than in stems in June. These findings suggest that the potential of the roots as a medicinal part. The roots were found to have highest purpurin content in October (2.406 mg g-1), whereas the mollugin content was highest in August (6.193 mg g-1). However, the purpurin content in August was only 0.029 mg g-1 lower than that in October, making August a suitable harvest period for R. cordifolia. The expression 1-deoxy-D-xylulose 5-phosphate synthase (dxs) and 1-deoxy-D-xylulose-5-phosphate reductorisomerase (dxr) genes in roots showed an upward trend. However, the expression level of dxr gene was significantly higher than dxs with the range of 60-518 times higher, indicating the important role of dxr gene. Through correlation and redundancy analyses, it was found that mollugin showed positive correlation with carbon contents and carbon-nitrogen ratio of aerial parts. Additionally, purpurin showed a positive correlation with the expression of both genes. As a result, mollugin is likely to be synthesized in the aerial parts and then stored in the roots, whereas purpurin might be synthesized in the stems and roots. These findings could provide cultivation guidelines for R. cordifolia.

Big-sized trees and higher species diversity improve water holding capacities of forests in northeast China.

High water-holding forests are essential for adapting to drought climates under global warming, and a central issue is which type of forests could conserve more water in the ecosystem. This paper explores how forest structure, plant diversity, and soil physics impact forest water-holding capacities. We investigated 720 sampling plots by measuring water-holding capacities from 1440 soil and litter samples, 8400 leaves, and 1680 branches and surveying 18,054 trees in total (28 species). Water-holding capacities were measured as four soil indices (Maxwc, maximum water-holding capacity; Fcwc, field water-holding capacity; Cpwc, soil capillary water-holding capacity; Ncpwc, non-capillary water-holding capacity), two litter metrics (Maxwcl, maximum water-holding capacity of litters; Ewcl, effective water-holding capacity of litters), and canopy interception (C, the sum of estimated water interception of all branches and leaves of all tree species in the plot). We found that water-holding capacity in the big-sized tree plots was 4-25 % higher in the litters, 54-64 % in the canopy, and 6-37 % in the soils than in the small-sized plots. The higher species richness increased all soil water-holding capacities compared to the lowest richness plot. Higher Simpson and Shannon-Wiener plots had 10-27 % higher Ewcl and C than the lowest plots. Bulk density had the strongest negative relations with Maxwc, Cpwc, and Fcwc, whereas field soil water content positively affected them. Soil physics, forest structure, and plant diversity explained 90.5 %, 5.9 %, and 0.2 % of the water-holding variation, respectively. Tree sizes increased C, Ncpwc, Ewcl directly (p < 0.05), and richness increased Ewcl directly (p < 0.05). However, the direct effects from the uniform angle index (tree distribution evenness) were balanced by their indirect effect from soil physics. Our findings highlighted that the mixed forests with big-sized trees and rich species could effectively improve the water-holding capacities of the ecosystem.

Responses of growth, photosynthesis and VOC emissions of Pinus tabulaeformis Carr. Exposure to elevated CO2 and/or elevated O3 in an urban area.

Responses of growth, photosynthesis and emission of volatile organic compounds of Pinus tabulaeformis exposed to elevated CO(2) (700 ppm) and O(3) (80 ppb) were studied in open top chambers. Elevated CO(2) increased growth, but it did not significantly (p > 0.05) affect net photosynthetic rate, stomatal conductance, chlorophyll content, the maximum quantum yield of photosystem II, or the effective quantum yield of photosystem II electron transport after 90 d of gas exposure. Elevated O(3) decreased growth (by 42.2% in needle weight and 25.8% in plant height), net photosynthetic rate and stomatal conductance after 90 d of exposure, but its negative effects were alleviated by elevated CO(2). Elevated O(3) significantly (p < 0.05) increased the emission rate of volatile organic compounds, which may be a helpful response to protect photosynthetic apparatus against O(3) damage.

Decrease in the residents' accessibility of summer cooling services due to green space loss in Chinese cities.

Urban green spaces (UGSs) reduce the surrounding temperature and create cooling areas as a buffer between people and high temperatures, thus helping residents adapt to the warming climate. However, the accessibility of UGS cooling services to the residents of cities remains largely unknown, which hinders decision-making regarding the formulation of climate adaptation and urban greening schemes. In the present study, we estimated the number of residents who accessed UGSs for cooling by analyzing the annual changes in such cooling areas during summer across 315 Chinese cities from 2003 to 2015. Approximately 93.3% of the cities showed significant decreasing trends (p < 0.05) of the total UGS area; as such the UGS coverage dropped from 12.23 ± 0.32% in 2003 to 7.69 ± 0.22% in 2015. Consequently, with the prevalent loss of UGS, the coverage of cooling spaces decreased from 32.55 ± 0.76% in 2003 to 24.39 ± 0.60% in 2015. This has formed a spatial mismatch between the growing urban population and the remaining UGSs. Accordingly, the number of residents of areas outside these cooling spaces increased by 4.23 million per year. In particular, the shortage of cooling services was more significant in cities with < 20,000 USD gross domestic product per capita and < 5 million residents than in the rest of the cities. To minimize the adverse impacts of increasing temperatures, focused greening plans are warranted, specifically in underdeveloped cities.