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Neutral aqueous organic redox flow batteries (AORFBs) hold the potential to facilitate the transition of renewable energy sources from auxiliary to primary energy, the commercial production of anolyte materials still suffers from insufficient performance of high-concentration and the high cost of the preparation problem. To overcome these challenges, this study provides a hydrothermal synthesis methodology and introduces the charged functional groups into hydrophobic naphthalene diimide cores, and prepares a series of high-performance naphthalene diimide anolytes. Under the synergistic effect of π-π stacking and H-bonding networks, the naphthalene diimide exhibits excellent structural stability and the highest water solubility (1.85â M for dex-NDI) reported to date. By employing the hydrothermal method, low-cost naphthalene diimides are successfully synthesized on a hundred-gram scale of $0.16â g-1 ($2.43â Ah-1), which is also the lowest price reported to date. The constructed full battery achieves a high electron concentration of 2.4â M, a high capacity of 54.4â Ah L-1, and a power density of 318â mW cm-2 with no significant capacity decay observed during long-duration cycling. These findings provide crucial support for the commercialization of AORFBs and pave the way for revolutionary developments in neutral AORFBs.
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Deeply electrolytic reduction of carbon dioxide (CO2) to high-value ethylene (C2H4) is very attractive. However, the sluggish kinetics of C-C coupling seriously results in the low selectivity of CO2 electroreduction to C2H4. Herein, we report a copper-based polyhedron (Cu2) that features uniformly distributed and atomically precise bi-Cu units, which can stabilize *OCCO dipole to facilitate the C-C coupling for high selective C2H4 production. The C2H4 faradaic efficiency (FE) reaches 51% with a current density of 469.4 mA cm-2, much superior to the Cu single site catalyst (Cu SAC) (~0%). Moreover, the Cu2 catalyst has a higher turnover frequency (TOF, ~520 h-1) compared to Cu nanoparticles (~9.42 h-1) and Cu SAC (~0.87 h-1). In situ characterizations and theoretical calculations revealed that the unique Cu2 structural configuration could optimize the dipole moments and stabilize the *OCCO adsorbate to promote the generation of C2H4.
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Multifunctional solar energy conversion offers a feasible strategy to solve energy, environmental and water crises. Herein, a series of platinum(II)-tethered chalcogenoviologens (PtL+ -EV2+ , E=S, Se, Te) is reported, which integrate the functions of photosensitizer, electron mediator and catalyst. PtL+ -EV2+ (particularly for PtL+ -SeV2+ )-based one-component solar H2 production could be triggered not only by EDTA, but also by facultative anaerobic and aerobic bacteria relying on a simplified mechanism, along with efficient antibacterial activities. In addition, by using real pool water, PtL+ -SeV2+ achieved multiple functions, including H2 production, antibacterial action and acid removal, which supplied a new strategy to solve various problems in real life via a single system.
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Antibacterianos/química , Calcogênios/química , Complexos de Coordenação/química , Fármacos Fotossensibilizantes/química , Platina/química , Energia Solar , Antibacterianos/síntese química , Antibacterianos/farmacologia , Calcogênios/farmacologia , Complexos de Coordenação/síntese química , Complexos de Coordenação/farmacologia , Escherichia coli/efeitos dos fármacos , Hidrogênio/química , Testes de Sensibilidade Microbiana , Fármacos Fotossensibilizantes/síntese química , Fármacos Fotossensibilizantes/farmacologia , Platina/farmacologia , Staphylococcus aureus/efeitos dos fármacosRESUMO
Hand-foot-and-mouth disease (HFMD) remains a major health concern in the Asia-Pacific regions, and its major causative agents include human enterovirus 71 (EV71) and coxsackievirus A16. A desirable vaccine against HFMD would be multivalent and able to elicit protective responses against multiple HFMD causative agents. Previously, we have demonstrated that a thermostable recombinant EV71 vaccine candidate can be produced by the insertion of a foreign peptide into the BC loop of VP1 without affecting viral replication. Here we present crystal structures of two different naturally occurring empty particles, one from a clinical C4 strain EV71 and the other from its recombinant virus containing an insertion in the VP1 BC loop. Crystal structure analysis demonstrated that the inserted foreign peptide is well exposed on the particle surface without significant structural changes in the capsid. Importantly, such insertions do not seem to affect the virus uncoating process as illustrated by the conformational similarity between an uncoating intermediate of another recombinant virus and that of EV71. Especially, at least 18 residues from the N terminus of VP1 are transiently externalized. Altogether, our study provides insights into vaccine development against HFMD.
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Capsídeo/química , Enterovirus Humano A/química , Vacinas de Partículas Semelhantes a Vírus/química , Sequência de Aminoácidos , Capsídeo/ultraestrutura , Cristalografia por Raios X , Enterovirus Humano A/genética , Enterovirus Humano A/imunologia , Dados de Sequência MolecularRESUMO
UNLABELLED: Human enterovirus 71 (EV71) and coxsackievirus A16 (CVA16) are the two major causative agents for hand-foot-and-mouth disease (HFMD). Previously, we demonstrated that a virus-like particle (VLP) for EV71 produced from Saccharomyces cerevisiae is a potential vaccine candidate against EV71 infection, and an EV71/CVA16 chimeric VLP can elicit protective immune responses against both virus infections. Here, we presented the crystal structures of both VLPs, showing that both the linear and conformational neutralization epitopes identified in EV71 are mostly preserved on both VLPs. The replacement of only 4 residues in the VP1 GH loop converted strongly negatively charged surface patches formed by portions of the SP70 epitope in EV71 VLP into a relatively neutral surface in the chimeric VLP, which likely accounted for the additional neutralization capability of the chimeric VLP against CVA16 infection. Such local variations in the amino acid sequences and the surface charge potential are also present in different types of polioviruses. In comparison to EV71 VLP, the chimeric VLP exhibits structural changes at the local site of amino acid replacement and the surface loops of all capsid proteins. This is consistent with the observation that the VP1 GH loop located near the pseudo-3-fold junction is involved in extensive interactions with other capsid regions. Furthermore, portions of VP0 and VP1 in EV71 VLP are at least transiently exposed, revealing the structural flexibility of the VLP. Together, our structural analysis provided insights into the structural basis of enterovirus neutralization and novel vaccine design against HFMD and other enterovirus-associated diseases. IMPORTANCE: Our previous studies demonstrated that the enterovirus 71 (EV71) virus-like particle (VLP) produced from yeast is a vaccine candidate against EV71 infection and that a chimeric EV71/coxsackievirus A16 (CVA16) VLP with the replacement of 4 amino acids in the VP1 GH loop can confer protection against both EV71 and CVA16 infections. This study reported the crystal structures of both the EV71 VLP and the chimeric EV71/CVA16 VLP and revealed that the major neutralization epitopes of EV71 are mostly preserved in both VLPs. In addition, the mutated VP1 GH loop in the chimeric VLP is well exposed on the particle surface and exhibits a surface charge potential different from that contributed by the original VP1 GH loop in EV71 VLP. Together, this study provided insights into the structural basis of enterovirus neutralization and evidence that the yeast-produced VLPs can be developed into novel vaccines against hand-foot-and-mouth disease (HFMD) and other enterovirus-associated diseases.
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Doença de Mão, Pé e Boca/prevenção & controle , Vacinas de Partículas Semelhantes a Vírus/isolamento & purificação , Vacinas Virais/isolamento & purificação , Sequência de Aminoácidos , Cristalografia por Raios X , Enterovirus/química , Enterovirus/genética , Enterovirus/imunologia , Enterovirus Humano A/química , Enterovirus Humano A/genética , Enterovirus Humano A/imunologia , Epitopos/química , Epitopos/imunologia , Humanos , Modelos Moleculares , Dados de Sequência Molecular , Conformação Proteica , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/metabolismo , Alinhamento de Sequência , Vacinas Sintéticas/química , Vacinas Sintéticas/imunologia , Vacinas Sintéticas/isolamento & purificação , Vacinas de Partículas Semelhantes a Vírus/química , Vacinas de Partículas Semelhantes a Vírus/imunologia , Vacinas Virais/química , Vacinas Virais/imunologiaRESUMO
UNLABELLED: Human enterovirus 71 (EV71) is the major causative agent of severe hand-foot-and-mouth diseases (HFMD) in young children, and structural characterization of EV71 during its life cycle can aid in the development of therapeutics against HFMD. Here, we present the atomic structures of the full virion and an uncoating intermediate of a clinical EV71 C4 strain to illustrate the structural changes in the full virion that lead to the formation of the uncoating intermediate prepared for RNA release. Although the VP1 N-terminal regions observed to penetrate through the junction channel at the quasi-3-fold axis in the uncoating intermediate of coxsackievirus A16 were not observed in the EV71 uncoating intermediate, drastic conformational changes occur in this region, as has been observed in all capsid proteins. Additionally, the RNA genome interacts with the N-terminal extensions of VP1 and residues 32 to 36 of VP3, both of which are situated at the bottom of the junction. These observations highlight the importance of the junction for genome release. Furthermore, EV71 uncoating is associated with apparent rearrangements and expansion around the 2- and 5-fold axes without obvious changes around the 3-fold axes. Therefore, these structures enabled the identification of hot spots for capsid rearrangements, which led to the hypothesis that the protomer interface near the junction and the 2-fold axis permits the opening of large channels for the exit of polypeptides and viral RNA, which is an uncoating mechanism that is likely conserved in enteroviruses. IMPORTANCE: Human enterovirus 71 (EV71) is the major causative agent of severe hand-foot-and-mouth diseases (HFMD) in young children. EV71 contains an RNA genome protected by an icosahedral capsid shell. Uncoating is essential in EV71 life cycle, which is characterized by conformational changes in the capsid to facilitate RNA release into host cell. Here we present the atomic structures of the full virion and an uncoating intermediate of a clinical C4 strain of EV71. Structural analysis revealed drastic conformational changes associated with uncoating in all the capsid proteins near the junction at the quasi-3-fold axis and protein-RNA interactions at the bottom of the junction in the uncoating intermediate. Significant capsid rearrangements also occur at the icosahedral 2- and 5-fold axes but not at the 3-fold axis. Taking the results together, we hypothesize that the junction and nearby areas are hot spots for capsid breaches for the exit of polypeptides and viral RNA during uncoating.
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Capsídeo/química , Enterovirus Humano A/química , Doença de Mão, Pé e Boca/virologia , Capsídeo/metabolismo , Proteínas do Capsídeo/química , Proteínas do Capsídeo/genética , Proteínas do Capsídeo/metabolismo , Cristalização , Enterovirus Humano A/genética , Enterovirus Humano A/metabolismo , Humanos , Modelos MolecularesRESUMO
Hand, foot, and mouth disease (HFMD) has caused significant morbidity and mortality in the Asia-Pacific regions, particularly in infants and young children. Coxsackievirus A16 (CA16) represents one of the major causative agents for HFMD, and the development of a safe and effective vaccine preventing CA16 infections has become a public health priority. In this study, we have developed a yeast system for the production of virus-like particles (VLPs) for CA16 by co-expressing P1 and 3CD of CA16 in Saccharomyces cerevisiae. These VLPs exhibit similarity in both protein composition and morphology as empty particles from CA16-infected cells. Immunization with CA16 VLPs in mice potently induced CA16-specific IgG and neutralization antibodies in a dose-dependent manner. IgG subclass isotyping revealed that IgG1 and lgG2b were dominantly induced by VLPs. Meanwhile, cytokine profiling demonstrated that immunization with VLPs significantly induced the secretion of IFN-γ, indicating potent cellular immune response. Furthermore, in vivo challenge experiments showed that passive immunization with anti-VLPs sera conferred full protection against lethal CA16 challenge in neonate mice. Taken together, our data demonstrated that VLPs produced in yeast might have the potential to be further developed as a vaccine candidate against HFMD.
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Enterovirus/genética , Enterovirus/imunologia , Saccharomyces cerevisiae/genética , Vacinas de Partículas Semelhantes a Vírus/imunologia , Vacinas Virais/imunologia , Animais , Animais Recém-Nascidos , Anticorpos Neutralizantes/sangue , Anticorpos Antivirais/sangue , Infecções por Coxsackievirus/prevenção & controle , Modelos Animais de Doenças , Imunização Passiva/métodos , Imunoglobulina G/sangue , Interferon gama/metabolismo , Leucócitos Mononucleares/imunologia , Camundongos , Dados de Sequência Molecular , RNA Viral/genética , Análise de Sequência de DNA , Vacinação/métodos , Vacinas Sintéticas/genética , Vacinas Sintéticas/imunologia , Vacinas Sintéticas/isolamento & purificação , Vacinas de Partículas Semelhantes a Vírus/genética , Vacinas de Partículas Semelhantes a Vírus/isolamento & purificação , Vacinas Virais/genética , Vacinas Virais/isolamento & purificaçãoRESUMO
A mesoscopic model based on the lattice Boltzmann method (LBM) is proposed to simulate the formation of Liesegang precipitation patterns and investigate the effects of gel on the morphology of the precipitates. In this model, nucleation is introduced on the basis of Ostwald's supersaturation theory, and subsequent crystal growth on the precipitate surface is simulated using a crystal growth model by taking into account the heterogeneous reaction on the surface of the precipitate. This model can capture the porous structures of the precipitates and can take into account the effects of the gel concentration and material by adjusting the gel porosity and nucleation threshold. The density of the precipitate nodes in the model is limited. A wide range of precipitation patterns is predicted under different gel concentrations, including regular bands, treelike patterns, and for the first time with numerical models, transition patterns between regular bands and treelike patterns. Both the spacing law and the width law are carefully investigated with different gel concentrations and gel materials, and the obtained α in the width law (w(n) ≈ x(n)(α), where x(n) and w(n) are the position and the width of the nth band, respectively) is in the range of 0.58-0.67, sandwiched by previously predicted ranges.
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Eletrólitos/química , Géis/química , Difusão , Hidrodinâmica , Modelos Moleculares , Conformação Molecular , Porosidade , Propriedades de SuperfícieRESUMO
In this work, we investigate the release of methane in quartz nanochannels through the method of displacement using carbon dioxide. Molecular dynamics (MD) simulations and theoretical analysis are performed to obtain the release percentage of methane for nanochannels of various diameters. It is found that both the pressure of CO2 and the channel size affect the release percentage of methane, which increases with increasing pressure of CO2 and channel diameter. Without CO2, the majority of methane molecules are adsorbed by the channel surface. When CO2 is injected into the channel, CO2 molecules replace many methane molecules due to the relatively strong molecular interactions between CO2 and the channel, which leads to the desorption of methane, reduces the energy barrier for the transport of methane, and consequently increases the release rate. Theoretical predictions using the kinetic energy of methane and the energy barrier inside the channel are also conducted, which are in good agreement with the MD simulations.
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[This retracts the article DOI: 10.1093/nsr/nwaa223.].
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In this work, the effects of external pressure on the release of methane through zeolite nanochannels are studied through molecular dynamics (MD) simulations. The release percentage of methane under three types of pressure loadings with various strengths and frequencies are obtained. Specifically, constant, sawtooth-shaped, and sinusoidal pressures are examined. As the pressure strength is increased, it is found that the release percentage first decreases and then increases significantly before finally approaching a constant. At sufficiently high pressures, the release percentage of methane under constant external pressure is about 65%, while it reaches over 90% for sawtooth-shaped and sinusoidal pressures. The loading frequency for periodic external pressures appears to be unimportant compared with the effect of the pressure strength. Theoretical predictions of the release percentage are made on the basis of the kinetic energy of methane molecules and the energy barrier inside the nanochannels, which are in good agreement with MD simulations.
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The thermal conductivity of fullerene-based superatomic crystals (SACs) is investigated using molecular dynamics simulations. The temperature-dependent predictions agree with the trends of previous measurements. The thermal conductivity behavior emerges as a result of the C60 molecule rotational dynamics and orientation, which are quantified using the root mean square displacements of the carbon atoms and the relative orientations of the C60s. At low temperatures, the C60s exhibit small rotations around equilibrium positions (i.e., librations). When the librating C60s are orientationally-ordered, as in the [C60] and [Co6Se8(PEt3)6][C60]2 SACs, thermal conductivity decreases with increasing temperature, as is typical for a crystal. When the librating C60s are orientationally-disordered, however, as in the [Co6Te8(PEt3)6][C60]2 SAC, thermal conductivity is lower and temperature independent, as is typical for an amorphous solid. At higher temperatures, where the C60s in all three SACs freely-rotate and are thus dynamically disordered, thermal conductivity is temperature independent. The abrupt changes driven by the C60 dynamics suggest that fullerene-based SACs can be designed to be thermal conductivity switches based on a variety of external stimuli.
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In this work, we investigate the effects of temperature and pore size on the release of methane in zeolite nanochannels through molecular dynamics (MD) simulations. The methane release percentage at different temperatures and for different zeolite structures is calculated. In all-silica MFI (silicalite-1) zeolite, it is found that the release percentage increases with increasing temperature roughly at a constant rate when the temperature is below 598 K. For higher temperatures, the release percentage reaches about 90% and remains almost constant. For other structures, the release percentage is greatly affected by the average pore size. The release percentage is determined by the temperature and energy barrier inside the pores. Based on the energy barriers obtained in MD simulations, theoretical predictions of the release percentage are made, which are in good agreement with numerical results.
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In this paper, an enthalpy-based multiple-relaxation-time (MRT) lattice Boltzmann (LB) method is developed for solid-liquid phase-change heat transfer in metal foams under the local thermal nonequilibrium (LTNE) condition. The enthalpy-based MRT-LB method consists of three different MRT-LB models: one for flow field based on the generalized non-Darcy model, and the other two for phase-change material (PCM) and metal-foam temperature fields described by the LTNE model. The moving solid-liquid phase interface is implicitly tracked through the liquid fraction, which is simultaneously obtained when the energy equations of PCM and metal foam are solved. The present method has several distinctive features. First, as compared with previous studies, the present method avoids the iteration procedure; thus it retains the inherent merits of the standard LB method and is superior to the iteration method in terms of accuracy and computational efficiency. Second, a volumetric LB scheme instead of the bounce-back scheme is employed to realize the no-slip velocity condition in the interface and solid phase regions, which is consistent with the actual situation. Last but not least, the MRT collision model is employed, and with additional degrees of freedom, it has the ability to reduce the numerical diffusion across the phase interface induced by solid-liquid phase change. Numerical tests demonstrate that the present method can serve as an accurate and efficient numerical tool for studying metal-foam enhanced solid-liquid phase-change heat transfer in latent heat storage. Finally, comparisons and discussions are made to offer useful information for practical applications of the present method.
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Síndrome Metabólica/epidemiologia , Parques Recreativos/provisão & distribuição , Características de Residência/estatística & dados numéricos , População Rural/estatística & dados numéricos , Adulto , Idoso , China/epidemiologia , Estudos de Coortes , Feminino , Humanos , Masculino , Síndrome Metabólica/etiologia , Pessoa de Meia-Idade , Prevalência , Adulto JovemRESUMO
In this paper, the thermal conductivity of dry Nafion 117 is measured by Hot Disk TPS2500 and a molecular dynamics calculation model for the proton exchange membrane is constructed by Materials Studio (MS) software platform to study its thermal conductivity. Cell structures of different water content of Nafion membrane at 300 K and 330 K are obtained, respectively. It is found that at the same temperature the predicted thermal conductivity of PEM increases with the water content, and at the same water content the predicted thermal conductivity decreases with the temperature. So both the water content and temperature are important factors affecting thermal conductivities of PEM.
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A pore-scale model based on the lattice Boltzmann (LB) method is developed for multiphase reactive transport with phase transitions and dissolution-precipitation processes. The model combines the single-component multiphase Shan-Chen LB model [X. Shan and H. Chen, Phys. Rev. E 47, 1815 (1993)], the mass transport LB model [S. P. Sullivan et al., Chem. Eng. Sci. 60, 3405 (2005)], and the dissolution-precipitation model [Q. Kang et al., J. Geophys. Res. 111, B05203 (2006)]. Care is taken to handle information on computational nodes undergoing solid-liquid or liquid-vapor phase changes to guarantee mass and momentum conservation. A general LB concentration boundary condition is proposed that can handle various concentration boundaries including reactive and moving boundaries with complex geometries. The pore-scale model can capture coupled nonlinear multiple physicochemical processes including multiphase flow with phase separations, mass transport, chemical reactions, dissolution-precipitation processes, and dynamic evolution of the pore geometries. The model is validated using several multiphase flow and reactive transport problems and then used to study the thermal migration of a brine inclusion in a salt crystal. Multiphase reactive transport phenomena with phase transitions between liquid-vapor phases and dissolution-precipitation processes of the salt in the closed inclusion are simulated and the effects of the initial inclusion size and temperature gradient on the thermal migration are investigated.