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Respir Res ; 18(1): 208, 2017 12 13.
Artigo em Inglês | MEDLINE | ID: mdl-29237464

RESUMO

BACKGROUND: Airway epithelial cells (AEC) act as the first line of defence in case of lung infections. They constitute a physical barrier against pathogens and they participate in the initiation of the immune response. Yet, the modalities of pathogen recognition by AEC and the consequences on the epithelial barrier remain poorly documented. METHOD: We investigated the response of primary human AEC to viral (polyinosinic-polycytidylic acid, poly(I:C)) and bacterial (lipopolysaccharide, LPS) stimulations in combination with the lung remodeling factor Transforming Growth Factor-ß (TGF-ß). RESULTS: We showed a strong production of pro-inflammatory cytokines (Interleukin (IL)-6, Tumor Necrosis Factor α, TNFα) or chemokines (CCL2, CCL3, CCL4, CXCL10, CXCL11) by AEC stimulated with poly(I:C). Cytokine and chemokine production, except CXCL10, was Toll Like Receptor (TLR)-3 dependent and although they express TLR4, we found no cytokine production after LPS stimulation. Poly(I:C), but not LPS, synergised with TGF-ß for the production of matrix metalloproteinase-9 (MMP-9) and fibronectin. Mechanistic analyses suggest the secretion of Wnt ligands by AEC along with a degradation of the cellular junctions after poly(I:C) exposure, leading to the release of ß-catenin from the cell membrane and stimulation of the Wnt/ß-catenin pathway. CONCLUSION: Our results highlight the cross talk between TGF-ß and TLR signaling in bronchial epithelium and its impact on the remodeling process.


Assuntos
Metaloproteinase 9 da Matriz/biossíntese , Mucosa Respiratória/metabolismo , Receptor 3 Toll-Like/biossíntese , Via de Sinalização Wnt/fisiologia , beta Catenina/metabolismo , Células Cultivadas , Quimiocinas/metabolismo , Citocinas/metabolismo , Humanos , Lipopolissacarídeos/farmacologia , Receptor Cross-Talk/efeitos dos fármacos , Receptor Cross-Talk/fisiologia , Mucosa Respiratória/efeitos dos fármacos , Fator de Crescimento Transformador beta/farmacologia , Via de Sinalização Wnt/efeitos dos fármacos
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