Noninvasive coronary angiography using 64-slice spiral computed tomography in an unselected patient collective: effect of heart rate, heart rate variability and coronary calcifications on image quality and diagnostic accuracy.

OBJECTIVE: The aim of this study was to assess the impact of heart rate, heart rate variability and calcification on image quality and diagnostic accuracy in an unselected patient collective. SUBJECTS AND METHODS: One hundred and two consecutive patients with known or suspected coronary artery disease underwent both 64-MSCT and invasive coronary angiography. Image quality (IQ) was assessed by independent observers using a 4-point scale from excellent (1) to non-diagnostic (4). Accuracy of MSCT regarding detection or exclusion of significant stenosis (>50%) was evaluated on a per segment basis in a modified AHA 13-segment model. Effects of heart rate, heart rate variability, calcification and body mass index (BMI) on IQ and accuracy were evaluated by multivariate regression. IQ and accuracy were further analysed in subgroups of significant predictor variables and simple regression performed to calculate thresholds for adequate IQ. RESULTS: Mean heart rate was 68.2+/-13.3 bpm, mean heart rate variability 11.5+/-16.0 beats per CT-examination (bpct) and median Agatston score 226.5. Average IQ score was 2+/-0.6 whilst diagnostic quality was obtained in 89% of segments. Overall sensitivity, specificity, PPV or NPV was 91.2%, 99.2%, 95.3% or 98.3%. According to multivariate regression, overall IQ was significantly related to heart rate and calcification (P=0.0038; P<0.0001). The effect of heart rate variability was limited to IQ of RCA segments (P=0.018); BMI was not related to IQ (P=0.52). Calcification was the only predictor variable with significant effect on the number of non-diagnostic segments (P<0.0001). In a multivariate regression, calcification was also the single factor with impact on diagnostic accuracy (P=0.0049). CONCLUSION: Whilst heart rate, heart rate variability and calcification all show an inverse correlation to IQ, severe calcium burden remains the single factor with translation of such effect into decrease of diagnostic accuracy.

Chopart's joint load during gait. In vitro study of 10 cadaver specimen in a dynamic model.

BACKGROUND: Chopart's joint is fundamental to foot function. Until today, intra-articular force and peak pressure has not been investigated under dynamic conditions. METHODS: The study used a cadaver model to measure intra-articular force and peak pressure with electronic sensors. Force was applied to extrinsic tendons via cables attached to computer-regulated hydraulic cylinders. A ground reaction force of 350 N was simulated in a tilting angle- and force-controlled translation stage. RESULTS: We observed a characteristic rising curve with a peak during push-off for intra-articular force and peak pressure. The increase of intra-articular force at the talonavicular and calcaneocuboid joint from a low level at heel-on varies up to a maximum of 174 N/149 N and a peak pressure of 3877 kPa/3396 kPa, respectively, at push-off. We observed highest loading at the dorsal aspect of the talonavicular joint and the plantar aspect of calcaneocuboid joint. CONCLUSION: The highest loading on Chopart's joint is attained during push-off. We observe higher force and peak pressure on the medial column of the foot compared to the lateral column. The higher load of the dorsal aspect of talonavicular joint and plantar aspect of calcaneocuboid joint confirms the theory of a previous described locking mechanism for forceful push-off.

[Internally cooled bipolar radiofrequency ablation: is a lower power output more effective?]. / Intern gekühlte bipolare Radiofrequenzablation: Ist eine niedrige Leistung effektiver?

PURPOSE: Evaluation of bipolar radiofrequency (RF) ablation using internally cooled electrodes in an ex-vivo experiment. MATERIALS AND METHODS: Bipolar RF ablations (n = 154) were performed in ex-vivo bovine liver. Both electrodes with a total length of the active tip of 4 cm were located on the same shaft of an internally cooled applicator. The power output was systematically varied between 20 and 100 watts (W). The energy application was continuous or modulated depending on the tissue resistance. In relationship to the maximum power output, the volume of coagulation was assessed. RESULTS: In continuous energy application the induced volume of coagulation was increased at lower power outputs up to 33.7 cm (3) (20 watts). Parallel to an increased volume of coagulation, the required duration of energy application was increased up to a maximum of 51.6 minutes. Modulation of the power output as a function of the tissue resistance enabled application of a wide range of power outputs (40 - 75 watts) leading to a comparable extent of coagulation with a maximum of 14.9 cm (3) (10 min.), 16.8 cm (3) (15 min.), and 19.1 cm (3) (20 min.). CONCLUSION: Continuous application of RF energy leads to an inverse relationship between volume of coagulation and power output. Modulation of the power output as a function of the tissue resistance enables application of a wider range of power outputs compared to continuous application of RF energy.

Changes in Chopart joint load following tibiotalar arthrodesis: in vitro analysis of 8 cadaver specimens in a dynamic model.

BACKGROUND: In the current discussion of surgical treatment of arthroses in the ankle joint, arthrodesis is in competition with artificial joint replacement. Up until now, no valid biomechanical findings have existed on the changes in intraarticular loads following arthrodesis. One argument against tibiotalar arthrodesis is the frequently associated, long-term degeneration of the talonavicular joint, which can be attributed to changes in biomechanical stresses. METHODS: We used a dynamic model to determine the changes in intraarticular forces and peak-pressure in the talonavicular joint and in the calcaneocuboid joint on 8 cadaver feet under stress in a simulated stance phase following tibiotalar arthrodesis. RESULTS: The change seen after arthrodesis was a tendency of relocation of average force and maximum pressure from the lateral onto the medial column of the foot. The average force increased from native 92 N to 100 N upon arthrodesis in the talonavicular joint and decreased in the calcaneocuboid joint from 54 N to 48 N. The peak pressure increased from native 3.9 MPa to 4.4 MPa in the talonavicular joint and in the calcaneocuboid joint from 3.3 MPa to 3.4 MPa. The increase of force and peak pressure on the talonavicular joint and decrease of force on the calcaneocuboid joint is statistically significant. CONCLUSION: The increase in imparted force and peak pressure on the medial column of the foot following tibiotalar arthrodesis, as was demonstrated in a dynamic model, biomechanically explains the clinically observed phenomenon of cartilage degeneration on the medial dorsum of the foot in the long term. As a clinical conclusion from the measurements, it would be desirable to reduce the force imparted on the medial column with displacement onto the lateral forefoot, say by suitable shoe adjustment, in order to achieve a more favourable long-term clinical result.

Determination of lysozyme in serum, urine, cerebrospinal fluid and feces by enzyme immunoassay.

Conjugates of human lysozyme and horseradish peroxidase (HRP) were prepared by means of the heterobifunctional reagent N-succinimidyl 3-(2-pyridyldithio) propionate. A conjugate containing 2 mol HRP/mol lysozyme was isolated by gel filtration and used as a labeled antigen in competitive enzyme immunoassays, in which anti-lysozyme rabbit IgG had been bound to wells of microtiter plates. The assay can detect as little as 1 microgram lysozyme/l. The following reference intervals have been established: 950-2450 micrograms/l for serum, 1.7-123 micrograms/l for urine, 17.6-118 micrograms/l for cerebrospinal fluid and 0.04-1.5 microgram/g for feces.

Randomised crossover trial of four nasal respiratory support systems for apnoea of prematurity in very low birthweight infants.

BACKGROUND: Apnoea of prematurity (AOP) is a common problem in preterm infants which can be treated with various modes of nasal continuous positive airway pressure (NCPAP) or nasal intermittent positive pressure ventilation (NIPPV). It is not known which mode of NCPAP or NIPPV is most effective for AOP. OBJECTIVE: To assess the effect of four NCPAP/NIPPV systems on the rate of bradycardias and desaturation events in very low birthweight infants. METHODS: Sixteen infants (mean gestational age at time of study 31 weeks, 10 males) with AOP were enrolled in a randomised controlled trial with a crossover design. The infants were allocated to receive nasal pressure support using four different modes for 6 h each: NIPPV via a conventional ventilator, NIPPV and NCPAP via a variable flow device, and NCPAP delivered via a constant flow underwater bubble system. The primary outcome was the cumulative event rate of bradycardias (< or =80 beats per minute) and desaturation events (< or =80% arterial oxygen saturation), which was obtained from cardio-respiratory recordings. RESULTS: The median event rate was 6.7 per hour with the conventional ventilator in NIPPV mode, and 2.8 and 4.4 per hour with the variable flow device in NCPAP and NIPPV mode, respectively (p value<0.03 for both compared to NIPPV/conventional ventilator). There was no significant difference between the NIPPV/conventional ventilator and the underwater bubble system. CONCLUSION: A variable flow NCPAP device may be more effective in treating AOP in preterm infants than a conventional ventilator in NIPPV mode. It remains unclear whether synchronised NIPPV would be even more effective.

[Loading of the tibiotalar joint and Chopart's joint following subtalar arthrodesis: dynamic cadaver study of 5 specimens]. / Belastung des oberen Sprunggelenkes und des Chopart-Gelenkes infolge der subtalaren Arthrodese: Untersuchung von 5 Kadaverpräparaten in einem dynamischen Modell.

AIM: Little has been known until now about biomechanical intra-articular changes in the condition of adjacent joints following subtalar arthrodeses. METHOD: We tested 5 cadaver specimens in a dynamic axially loaded foot model under computer-regulated load application on 9 extrinsic tendons of the foot regarding changes in load under native conditions and following an anatomically fixed subtalar arthrodesis. RESULTS: We saw an averaged native force transmission in the talonavicular joint of 87 N, increasing post-arthrodesis to 92 N. In the calcaneocuboid joint we measured an increase from 72 N to 74 N. For peak pressure, the increase was from native 3152 kPa to 3286 kPa in the talonavicular joint and in the calcaneocuboid joint we recorded a reduction from 3282 kPa to 3277 kPa. The effects are not striking from a statistical viewpoint. However, we observed a reduction in force from native 103 N to 90 N and in peak pressure from 4778 kPa to 4261 kPa in the ankle. When the ankle was divided into discrete zones in the sagittal and frontal planes there was no evidence of load displacement. CONCLUSION: Our measurements show that anatomically fixed arthrodeses of the talocalcanear joint do not lead to any striking increase in loads on adjacent joints. Any adjacent segment degeneration following subtalar arthrodeses may be the result of changes in joint kinematics or non-anatomically reconstructed hindfoot positions.