RESUMO
BACKGROUND: In medicine, critical thinking is required for managing and tolerating medical uncertainty, as well as solving professional problems and treating diseases. However, the core of Confucianism, teacher-centered and exam-oriented settings in middle and high school education may pose challenges to developing critical thinking in Han Chinese or Taiwanese students. Students may be adversely affected by these pedagogies since student-centered settings were more effective in stimulating their critical and reflective thinking, as well as a sense of responsibility, in the ever-changing world. Therefore, guiding students with less stable foundations of critical thinking might require a different approach. A review article highlighted the potential utility of the Socratic method as a tool for teaching critical thinking in the healthcare field. The method involves posing a series of questions to students. More importantly, medical students and residents in clinical teaching are familiar with the method. Almost all healthcare students must complete a biochemistry laboratory course as part of their basic science training. Thus, we aimed to train students to develop critical thinking in the biochemistry laboratory course by using learning sheets and teacher guidance based on the Socratic method and questioning. METHOD: We recruited second-year students from a medical school, of whom 32 had medical science and biotechnology majors (MSB), 27 had pharmaceutical science majors (PS), and 85 were medical undergraduate (MU) students. An exercise in critical thinking was conducted during a biochemistry laboratory course, which consisted of five different biochemical experiments, along with learning sheets that contained three or four critical thinking questions. Then, the teacher evaluated the students' ability to think critically based on nine intellectual dimensions (clarity, accuracy, precision, relevance, depth, breadth, logic, fairness, and significance) based on the universal intellectual standards developed by Prof. Linda Elder and Richard Paul. In the following analysis, regression models and multivariate analysis were used to determine how students improved over time, and trajectory analysis were carried out in order to observe the trends in students' critical thinking skills construction. RESULTS: Clarity and logic dimensions were identified as the key elements to facilitate the development of critical thinking skills through learning sheets and teacher guidance in students across all three different healthcare majors. The results showed that metacognitive monitoring via Socratic questioning learning sheets have demonstrated potential encourage students to develop critical thinking skills in all dimensions. Another unique contribution of current study was present the heterogeneous learning patterns and progress trajectories of clarity and logic dimensions within classes. CONCLUSION: Using the Socratic learning model could effectively develop students' critical thinking skills so they can more effectively care for their patients.
Assuntos
Bacharelado em Enfermagem , Estudantes de Medicina , Humanos , Idoso , Pensamento , Resolução de Problemas , Bacharelado em Enfermagem/métodos , Atenção à SaúdeRESUMO
Group B streptococcus (GBS) is a common asymptomatic colonizer in acidic vagina of pregnant women and can transmit to newborns, causing neonatal pneumonia and meningitis. Biofilm formation is often associated with bacterial colonization and pathogenesis. Little is known about GBS biofilm and the effect of environmental stimuli on their growth along with biofilm formation. The objective of this study was to investigate the survival and biofilm formation of GBS, isolated from pregnant women, in nutrient-limited medium under various pH conditions. Growth and survival experiments were determined by optical density and viable counts. Crystal violet staining, scanning electron microscopy, and atomic force microscopy (AFM) were used to analyze the capacity of biofilm production. Our results showed that GBS isolates proliferated with increasing pH with highest maximum specific growth rate (µmax) at pH 6.5, but survived at pH 4.5 for longer than 48 h. Biofilm formation of the 80 GBS isolates at pH 4.5 was significantly higher than at pH 7.0. This difference was confirmed by two other methods. The low elastic modulus obtained from samples at pH 4.5 by AFM revealed the softness of biofilm; in contrast, little or no biofilm was measured at pH 7.0. Under acidic pH, the capability of biofilm formation of serotypes III and V showed statistically significant difference from serotypes Ia and Ib. Our finding suggested that survival and enhanced biofilm formation at vaginal pH are potentially advantageous for GBS in colonizing vagina and increase the risk of vaginosis and neonatal infection.
Assuntos
Biofilmes , Streptococcus agalactiae/fisiologia , Vagina/microbiologia , Adulto , Feminino , Perfilação da Expressão Gênica , Humanos , Concentração de Íons de Hidrogênio , Gravidez , Estudos Prospectivos , Sorotipagem , Streptococcus agalactiae/isolamento & purificação , Streptococcus agalactiae/ultraestruturaRESUMO
Streptococcus pyogenes (group A streptococcus [GAS]) is a versatile human pathogen, and emm1/sequence type 28 (ST28) is the most frequently isolated type from GAS infections. The emm1/ST28 strain is associated with necrotizing fasciitis and streptococcal toxic shock syndrome. Growth-phase regulation is one of the important regulatory mechanisms in GAS, which controls gene expression at restricted phases of growth. CovRS, a two-component regulatory system, is considered the regulator of streptococcal pyrogenic exotoxin B (SpeB) and is thought to be activated in the exponential phase of growth. In the present study, Northern hybridization analysis showed that 52% of the analyzed GAS strains expressed covR at the exponential phase, but 48% of the strains expressed covR at the early stationary phase of growth. Strains transcribing covR at the early stationary phase showed better growth and earlier SpeB expression than the other group of strains. Multilocus sequence typing and pulsed-field gel electrophoresis analysis showed only emm1/ST28 strains (which comprise a clonal cluster) were expressing covR at the early stationary phase of growth, indicating that emm1/ST28 strains have special characteristics which may be related to their worldwide distribution.
Assuntos
Antígenos de Bactérias/genética , Proteínas da Membrana Bacteriana Externa/genética , Proteínas de Bactérias/biossíntese , Proteínas de Bactérias/metabolismo , Proteínas de Transporte/genética , Exotoxinas/biossíntese , Regulação Bacteriana da Expressão Gênica , Proteínas Repressoras/metabolismo , Streptococcus pyogenes/fisiologia , Northern Blotting , Análise por Conglomerados , Impressões Digitais de DNA , DNA Bacteriano/genética , Eletroforese em Gel de Campo Pulsado , Perfilação da Expressão Gênica , Genótipo , Humanos , Análise de Sequência de DNA , Transdução de Sinais , Infecções Estreptocócicas/microbiologia , Streptococcus pyogenes/classificação , Streptococcus pyogenes/genética , Streptococcus pyogenes/isolamento & purificaçãoRESUMO
Of 1,994 group B streptococcal isolates collected, 26 (1.3%) of the isolates were resistant to levofloxacin, and cross-resistance to other fluoroquinolones was observed. The emergence and prevalence of high-level fluoroquinolone resistance in genetically unrelated isolates were linked to the presence of gyrA, parC, and parE triple mutations in each isolate.
Assuntos
Fluoroquinolonas/farmacologia , Levofloxacino , Ofloxacino/farmacologia , Streptococcus agalactiae/efeitos dos fármacos , Proteínas de Bactérias/genética , DNA Girase/genética , DNA Topoisomerase IV/genética , Farmacorresistência Bacteriana Múltipla/genética , Testes de Sensibilidade Microbiana , Mutação , Streptococcus agalactiae/genética , Streptococcus agalactiae/isolamento & purificação , TaiwanRESUMO
Atherosclerosis and resultant peripheral arterial disease (PAD) are common complications in patients with type 2 diabetes mellitus or end-stage renal disease and in elderly patients. The prevalence of PAD is higher in patients receiving haemodialysis therapy. For early assessment of arterial occlusion using bilateral photoplethysmography (PPG), such as changes in pulse transit time and pulse shape, bilateral timing differences could be used to identify the risk level of PAD. Hence, the authors propose a discrete fractional-order integrator to calculate the bilateral area under the systolic peak (AUSP). These indices indicated the differences in both rise-timing and amplitudes of PPG signals. The dexter and sinister AUSP ratios were preliminarily used to separate the normal condition from low/high risk of PAD. Then, transition probability-based decision-making model was employed to evaluate the risk levels. The joint probability could be specified as a critical threshold, < 0.81, to identify the true positive for screening low or high risk level of PAD, referring to the patients' health records. In contrast to the bilateral timing differences and traditional methods, the proposed model showed better efficiency in PAD assessments and provided a promising strategy to be implemented in an embedded system.
Assuntos
Técnicas de Apoio para a Decisão , Técnicas de Diagnóstico Cardiovascular , Modelos Estatísticos , Doença Arterial Periférica/diagnóstico , Doença Arterial Periférica/etiologia , Fotopletismografia , Diálise Renal/efeitos adversos , Algoritmos , Simulação por Computador , Tomada de Decisões Assistida por Computador , Técnicas de Diagnóstico Cardiovascular/instrumentação , Estudos de Viabilidade , Humanos , Reprodutibilidade dos Testes , Sensibilidade e EspecificidadeRESUMO
Since the advent of pandemic of the human immunodeficiency virus infection, the possible pathogens responsible for acute meningoencephalitis have broadened. Human immunodeficiency virus itself can cause acute meningoencephalitis, and the immunocompromise associated with human immunodeficiency virus infection predisposes the infected patients to acute meningoencephalitis caused by a variety of other infectious or non-infectious etiologies. Here reported are 2 cases of acute meningoencephalitis with history of blood transfusion and travel to southeast Asia; both are positive for screening tests of human immunodeficiency virus infection. One of the pathogen causing central nervous system infection, Mycobacterium tuberculosis, was identified by polymerase chain reaction; the other left undiagnosed. It is known that patients of human immunodeficiency virus infection or acquired immunodeficiency syndrome can present with acute central nervous system infection. The need for routine screening of human immunodeficiency virus antibody is currently under debate; nevertheless, the possibility of human immunodeficiency virus infection has to be kept in mind in patients with acute meningoencephalitis.
Assuntos
Infecções por HIV/diagnóstico , Meningoencefalite/diagnóstico , Doença Aguda , Adulto , Western Blotting , Cefazolina/farmacologia , Cefazolina/uso terapêutico , Gentamicinas/farmacologia , Gentamicinas/uso terapêutico , Infecções por HIV/patologia , HIV-1/isolamento & purificação , Humanos , Masculino , Meningoencefalite/virologia , Pessoa de Meia-Idade , Mycobacterium tuberculosis/isolamento & purificação , Reação em Cadeia da Polimerase , Tuberculose/diagnósticoRESUMO
Due to reduced antibiotic consumption in Taiwan, erythromycin resistance rate had decreased in Streptococcus pyogenes, but it increased in Streptococcus pneumoniae. The objectives of the present study were (1) to determine the erythromycin and clindamycin resistance rate and minimum inhibitory concentration (MIC) of the group B streptococcus (GBS) clinical isolates, and (2) to investigate the mechanism responsible for the macrolide, lincosamide, and group B streptogramin (MLS(B)) resistance. A total of 1,395 GBS isolates were collected from June 2001 to April 2007. Forty-four percent of the GBS isolates were resistant to erythromycin, and 39% were resistant to clindamycin. The annual erythromycin resistance rate increased from 32% in 2001 to 51% in 2004; a significant decrease was observed in 2005 (47%), 2006 (42%), and 2007 (38%). Percentage of erythromycin-resistant isolates with erm(B) gene significantly increased from 72% in 2001 to 90% in 2007. We found that the plasmid encoded zeta gene was present in 13% of the resistant isolates, along with erm(B). When compared to our previous study (1991 to May 2001), the overall erythromycin resistance rate increased from 30% to 44%. erm(B) was the major resistant determinant, and zeta toxin encoding plasmid has a limited role in mediating erythromycin resistance unlike in GAS isolates as reported earlier.
Assuntos
Antibacterianos/farmacologia , Farmacorresistência Bacteriana , Eritromicina/farmacologia , Hospitais Universitários , Infecções Estreptocócicas/epidemiologia , Streptococcus agalactiae/efeitos dos fármacos , Sequência de Aminoácidos , Proteínas de Bactérias/genética , Clindamicina/farmacologia , Farmacorresistência Bacteriana/genética , Humanos , Testes de Sensibilidade Microbiana , Dados de Sequência Molecular , Prevalência , Infecções Estreptocócicas/microbiologia , Streptococcus agalactiae/genética , Taiwan/epidemiologiaRESUMO
The average number of tandem repeats of the rib gene (which encodes the Rib surface protein) in invasive group B streptococci from 29 neonates was smaller than that from 20 adults (6.8 and 8.6, respectively; P<0.05), implying a distinct contribution of immunity toward this age-related variation.