[The effect of S-adenosyl-L-methionine sulfate tosylate (FO-1561) on survival time in various brain damage models].

The effect of S-adenosyl-L-methionine sulfate tosylate (FO-1561) on survival time in various brain damage models (cerebral anoxia or ischemia) was studied. 1) In KCN-induced or normobaric anoxia of ddY mice, FO-1561 (30-100 mg/kg as amount of S-adenosyl-L-methionine) administered intravenously 15-30 min prior to the treatment showed significant increase of survival time dose-dependently. 2) In asphyxic anoxia of Wistar rats, FO-1561 (100 mg/kg) administered intravenously 15 min prior to the treatment (cessation of artificial respiration) delayed the time until the disappearance of electrocorticogram. 3) In cerebral ischemia of Mongolian gerbils, FO-1561 (50 mg/kg) injected five times at 1 hr interval intraperitoneally 3 hr after the unilateral ligation of common carotid arteries showed significant increase of survival time. These results suggested that FO-1561 may be effective in ameliorating cerebral anoxic or ischemic damage, without observing any side effects like sedation and motor depression which pentobarbital showed with the effective doses in these damage models.

Desempenho e características da carcaça de novilhos terminados em pastejo rotacionado ou em confinamento / Performance and carcass characteristics of steers finished in rotational grassing or feedlot

Foram avaliados o desempenho e as características de carcaça de 36 novilhos Brahman (Bh), Brangus (Bg) e Hereford adaptado (He), terminados em pastejo com suplementação (PSu) ou em confinamento (Conf). Os animais foram abatidos quando apresentavam espessura de gordura maior do que 4mm. Na dieta do Conf, a relação volumoso:concentrado foi de 60:40 (com base na matéria seca), continha 13 por cento de proteína bruta (PB) e 62 por cento de nutrientes digestíveis totais (NDT). O capim-mombaça continha, em média, 7 por cento de PB e 54 por cento de NDT, e o suplemento 24 por cento de PB e 76 por cento de NDT. Foi observada diferença (P<0,05) no sistema de terminação para o ganho de peso médio diário, com 1,200kg/cab/dia para Conf e 0,675kg/cab/dia para Psu. Os novilhos Bh e Bg pesaram, em média, 445,8kg, e o rendimento da carcaça quente foi de 58,4 por cento, valores maiores que os 399,1kg e 55,4 por cento obtidos pelos animais He. A área de olho de Longissimus foi maior para os animais Bg, 76,42cm². A maciez, 3,71 vs 4,89, e a espessura de gordura subcutânea, 4,64 vs 3,39, foram melhores nos animais terminados em Conf do que nos animais em Psu, respectivamente.

The productive performance and carcass of the 36 Brahman (Bh), Brangus (Bg) and adapted Hereford (He) steers finished on feedlot (FD) or on rotational grazing with supplementation system (GS) were evaluated. The animals were slaughtered when the subcutaneous fat thickness was over 4mm. The feedlot diet had a forage: concentrate of 60:40 (in dry matter) 13 percent crude protein (CP), 62 percent of NDT. The mombaça gras CP level was 7 percent and 54 percent of NDT. The supplement had supplied 24 percent of CP and 76 percent of NDT. A significant difference was observed for the average daily gain, being 1,200kg/head/day for FD steers and 0.675kg/head/day for GS steers. The effect of breed on slaughter weight was observed, being 445.8kg for Bh and Bg and 399.19kg for He. The hot dressing percentage was higher for Bh and Bg than for He, 58.4 percent vs 55.4 percent. The rib eye area was higher for Bg, 76.42cm² and tenderness measured by the shear force was lower for FD steers, at 3.71 than for GS steers, at 4.89. The subcutaneous fat thickness was higher for FD steers, at 4.64 than for GS steers, at 3.39mm.

[Effect of S-adenosyl-L-methionine sulfate tosylate (FO-1561) on functional recovery after transient cerebral ischemia in rats].

The present investigation was undertaken to examine the recovery of functions, spontaneous EEG activity and cerebral ATP content during blood recirculation following cerebral ischemia in rats. The ischemia was induced by bilateral carotid artery 30 min-occlusion 24 hr after the permanent electrocauterization of bilateral vertebral arteries. EEG spectral analysis was performed by the Berg transform. The recovery of EEG activity was assessed by the time elapsing from the onset of blood recirculation until the delta (0.5-4 Hz), theta (4-8 Hz), alpha (8-13 Hz) or beta (13-32 32 Hz) band reappeared and the relative proportion of the four bands at 45 and 60 min after recirculation. Following the 20 min recirculation, the brains were frozen in situ with liquid nitrogen and then stored at -80 degrees C for later extraction for analysis. Concentrations of ATP in the cerebral cortex were determined with enzymatic methods. In animals administered FO-1561 (10 approximately 100 mg/kg, i.v.) simultaneously with blood recirculation, the reduction of time elapsing until reappearance of the theta, alpha and beta bands, the decrease in proportion of the delta band and the increase in that of the theta, alpha and beta bands which indicated the facilitation of transition to the normal state were observed. In animals administered FO-1561 (100 mg/kg), the improvement of ATP content was detected. These results demonstrate that FO-1561 may have a beneficial effect on the recovery from dysfunctions due to cerebrovascular disorder.