RESUMO
OBJECTIVES: This study tested whether host genotypes of the tumor necrosis factor-alpha (TNF-alpha) promoter single nucleotide polymorphism (SNP) could determine clinical and histological outcomes after Helicobacter pylori infection. METHODS: A total of 524 dyspeptic patients, 424 with and 100 without H. pylori infection, were checked for TNF-alpha promoter SNP over the locus on -1031(T/C), -863(C/A), -857(C/T), -806(C/T), and -308(G/A) by sequence-specific oligonucleotide probe. Each patient received panendoscopy to take gastric biopsy to detect H. pylori infection and its related histology using the updated Sydney's system. Gastric TNF-alpha expressions were stained by immunohistochemistry. RESULTS: In H. pylori-infected patients, -1031C or -863A carriers of TNF-alpha promoter had more severe gastric neutrophil infiltration and TNF-alpha gastric staining than individuals with -1031TT or -863CC genotype, respectively (p<0.05). The multivariate logistic regression verified both -1031C and -863A carriers were independent risk factors to have duodenal ulcers and gastric ulcer without IM in the H. pylori-infected hosts (p<0.05). As compared to -863CC and -1031TT genotype combinations, the ulcer risk after H. pylori infection was 2.46 (95% CI: 1.32-4.59, pAssuntos
DNA/genética
, Mucosa Gástrica/metabolismo
, Infecções por Helicobacter/complicações
, Polimorfismo de Nucleotídeo Único
, Regiões Promotoras Genéticas/genética
, Úlcera Gástrica/etiologia
, Fator de Necrose Tumoral alfa/genética
, Biópsia
, Úlcera Duodenal/etiologia
, Úlcera Duodenal/metabolismo
, Úlcera Duodenal/patologia
, Endoscopia do Sistema Digestório
, Feminino
, Mucosa Gástrica/microbiologia
, Mucosa Gástrica/patologia
, Regulação da Expressão Gênica/fisiologia
, Marcadores Genéticos
, Genótipo
, Infecções por Helicobacter/metabolismo
, Infecções por Helicobacter/patologia
, Helicobacter pylori/isolamento & purificação
, Humanos
, Imuno-Histoquímica
, Masculino
, Pessoa de Meia-Idade
, Neutrófilos/patologia
, Reação em Cadeia da Polimerase
, Úlcera Gástrica/metabolismo
, Úlcera Gástrica/patologia
, Fator de Necrose Tumoral alfa/metabolismo