Herbal medicinal uses and their practices in human health care and livestock from district Haripur, Khyber Pakhtunkhwa, Pakistan.

BACKGROUND: In light of the increasing need, for global health care, traditional medical knowledge is receiving considerable attention in order to fulfil the public health needs not only for human health but also animal health. OBJECTIVES: Thus, the aim of the study is to explore ethnomedicinal uses of plants in human and livestock health care of the study area. METHODS: Data were gathered through arranged surveys and meeting techniques by focusing on 80 sources in the investigation region during 2018-2020. Quantitative ethnobotanical indices were calculated. RESULTS: Eighty plant species (33 herbs, 21 shrubs, 24 trees and 2 climbers) belonging to 50 families, distributed in 74 genera, were being used in the study area. Forty-nine medicinal plants in the treatment of 42 human diseases under 52 natural recipes and 40 plants in 34 livestock diseases under 40 recipes were used, in which 9 plants are used commonly in ethnopharmacy and veterinary medicine. Jaccard index was used to predict the similarity and dissimilarity among cities of Pakistan and reflected the novel use of medicinal plants. Results showed that Boerhavia diffusa, Centella asiatica, Morus nigra, Nasturtium officinale, Rumex hastatus and Sageretia thea have the higher use value (UV). Comparative analysis with other studies strongly reflected the novel use of these plants because of the deep-rooted and unique sociocultural setup of study area. CONCLUSIONS: Our research shows that the wild plants used in the study area are extremely varied, both in terms of species and function, and folk medicine is one of the main health care systems in the area.

Effect of α-Terpineol on Chicken Meat Quality during Refrigerated Conditions.

The present study was designed to evaluate the in vitro antimicrobial properties of nine bioactive compounds (BACs). Applying the disc paper and minimum inhibitory concentration (MIC) assays, we found that the BACs with the widest spectrum of in vitro antibacterial activity against the studied bacteria were carvacrol and α-terpineol (αTPN). Subsequently, αTPN was selected and applied at different concentrations into the fresh minced chicken meat. The meat was then vacuum packaged and stored for 14 days at 4 °C. Physicochemical properties, lipid oxidation (thiobarbituric acid reactive substances, TBARS), electronic-nose-based smell detection, and microbiological characteristics were monitored. At day 14, meat treated with higher concentrations of αTPN (MIC-2 and MIC-4) exhibited a significantly increased pH and lightness (L*), increased yellowness (b*), decreased redness (a*), caused a significant decrease in water holding capacity (WHC), and decreased lipid oxidation by keeping TBARS scores lower than the control. Although αTPN showed perceptibly of overlapped aroma profiles, the E-nose was able to distinguish the odor accumulation of αTPN between the different meat groups. During the 2-week storage period, αTPN, particularly MIC-4, showed 5.3 log CFU/g reduction in aerobic mesophilic counts, causing total inhibition to the Pseudomonas lundessis, Listeria monocytogenes, and Salmonella Typhimurium. These promising results highlight that αTPN is exploitable to improve the shelf life and enhance the safety of meat and meat products.