RESUMO
The genome of a densovirus of a major phytophagous pest, Pseudoplusia includens, was analyzed. It contained 5,990 nucleotides (nt) and included inverted terminal repeats of 540 nt with terminal Y-shaped hairpins of 120 nt. Its DNA sequence and ambisense organization with 4 typical open reading frames demonstrated that it belonged to the genus Densovirus in the subfamily Densovirinae of the family Parvoviridae.
Assuntos
Densovirus/genética , Genoma Viral/genética , Mariposas/virologia , Animais , Sequência de Bases , Densovirus/classificação , Dados de Sequência Molecular , Fases de Leitura Aberta/genética , Análise de Sequência de DNA , Sequências Repetidas Terminais/genéticaRESUMO
The sequence of Junonia coenia densovirus was the first densovirus genome sequence published, but the first published sequence contained incomplete inverted terminal repeats and ambiguous nucleotides or indels leading to an incorrect map of the open reading frames. Our sequencing of clones of the complete genome demonstrated that this virus is closely related to other viruses in the Densovirus genus.