RESUMO
The present study is the first report of fingerprinting on three Chilean Nothofagus species using ISSR and RAPD markers; 61 Nothofagus nervosa, 32 Nothofagus obliqua and 32 Nothafagus dombeyi individual trees, sampled from collection and natural sites, were analyzed. Among 45 primers tested, the 6 ISSR and 6 RAPD primers selected for the analysis generated a total of 63 ISSR and 42 RAPD fragments. A high proportion of polymorphic bands, ranging from 97% and 80%, was found using both markers. A similar number of private and marker bands was generated by both markers in all the species examined and one discriminant ISSR fragment was obtained for N. dombeyi. Jaccard and Dice similarity indices were used to evaluate pairwise genetic divergence; cluster analysis of the similarity matrices was performed to estimate the intra- and inter-specific genetic diversity, and PCA analysis was employed to evaluate the resolving power of the markers to differentiate between the species. These analyses, carried out for both markers, allowed us to identify three main groups corresponding to the three Nothofagus species. The results of the present study can be seen as a starting point for future researches on the population and evolutionary genetics of these species.
RESUMO
El año 2007 se inicia un Programa de Formación de Médicos Especialistas Básicos para la Atención Primaria de Salud, con un carácter experimental, financiado por el Ministerio de Salud, con la participación académica de la Facultad de Medicina de la Universidad de Chile y con el involucramiento de dos municipios de la Región Metropolitana. Se entrega información acerca de los antecedentes históricos del Programa, la doctrina que lo inspira, su evolución desde el inicio hasta hoy, el estado actual del mismo y sus perspectivas de desarrollo a futuro. Se concluye que es un Programa consolidado, que tiene el carácter de una Política de Estado y que es bien evaluado por todas las contrapartes involucrada. Por todo lo anterior se vislumbran perspectivas de crecimiento y de expansión del mismo a futuro y asimismo se identifican las dificultades que podrían existir para ello.
In 2007, a new Programme of Basic Specialists for Urban PHC is implemented by the Ministry of Health, with the participation of the Faculty of Medicine of the University of Chile in two municipalities of the Metropolitan Region. This Programme is based on previous national experience. At present he Programme is consolidated and is a new State Policy in the field of Medical Health Care Resources. The results so far are encouraging and its expansion, including the incorporation of new medical specialties, is envisaged.