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J Biol Chem ; 292(47): 19392-19399, 2017 11 24.
Artigo em Inglês | MEDLINE | ID: mdl-28986450

RESUMO

Signal-transducing adaptor family member-2 (STAP-2) is an adaptor protein that regulates various intracellular signaling pathways and promotes tumorigenesis in melanoma and breast cancer cells. However, the contribution of STAP-2 to the behavior of other types of cancer cells is unclear. Here, we show that STAP-2 promotes tumorigenesis of prostate cancer cells through up-regulation of EGF receptor (EGFR) signaling. Tumor growth of a prostate cancer cell line, DU145, was strongly decreased by STAP-2 knockdown. EGF-induced gene expression and phosphorylation of AKT, ERK, and STAT3 were significantly decreased in STAP-2-knockdown DU145 cells. Mechanistically, we found that STAP-2 interacted with EGFR and enhanced its stability by inhibiting c-CBL-mediated EGFR ubiquitination. Our results indicate that STAP-2 promotes prostate cancer progression via facilitating EGFR activation.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Proliferação de Células , Receptores ErbB/química , Fosfoproteínas/metabolismo , Neoplasias da Próstata/patologia , Animais , Receptores ErbB/metabolismo , Humanos , Masculino , Camundongos Endogâmicos BALB C , Fosforilação , Neoplasias da Próstata/metabolismo , Estabilidade Proteica , Transdução de Sinais , Células Tumorais Cultivadas , Ubiquitinação , Ensaios Antitumorais Modelo de Xenoenxerto
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