High-speed ultrasound imaging of bubbly flows and shear waves in soft matter.

In this methods paper, we explore the capabilities of high-speed ultrasound imaging (USI) to study fast varying and complex multi-phase structures in liquids and soft materials. Specifically, we assess the advantages and the limitations of this imaging technique through three distinct experiments involving rapid dynamics: the underwater flow induced by an external jet, the dissolution of sub-micron bubbles in water, and the propagation of shear waves in a soft elastic material. The phenomena were simultaneously characterized using optical microscopy and USI. In water, we use compounded USI for tracking a multi-phase flow produced by a jetting bubble diving into a liquid pool at speeds around 20 m s-1. These types of jets are produced by focusing a single laser pulse below the liquid surface. Upon breakup, they create a bubbly flow that exhibits high reflectivity to the ultrasound signal, enabling the visualization of the subsequent turbulent flow. In a second experiment, we demonstrate the potential of USI for recording the diffusive shrinkage of micro- and nanobubbles in water that could not be optically resolved. Puncturing an elastic material with a liquid jet creates shear waves that can be utilized for elastography measurements. We analysed the shape and speed of shear waves produced by different types of jetting bubbles in industrial gelatin. The wave characteristics were simultaneously determined by implementing particle velocimetry in optical and ultrasound measurements. For the latter, we employed a novel method to create homogeneously distributed micro- and nanobubbles in gelatin by illuminating it with a collimated laser beam.

Shear wave generation from non-spherical bubble collapse in a tissue phantom.

Elastography is a non-invasive technique to detect tissue anomalies via the local elastic modulus using shear waves. Commonly shear waves are produced via acoustic focusing or the use of mechanical external sources, shear waves may result also naturally from cavitation bubbles during medical intervention, for example from thermal ablation. Here, we measure the shear wave emitted from a well-controlled single laser-induced cavitation bubble oscillating near a rigid boundary. The bubbles are generated in a transparent tissue-mimicking hydrogel embedded with tracer particles. High-speed imaging of the tracer particles and the bubble shape allow quantifying the shear wave and relate it to the bubble dynamics. It is found that different stages of the bubble dynamics contribute to the shear wave generation and the mechanism of shear wave emission, its direction and the efficiency of energy converted into the shear wave depend crucially on the bubble to wall stand-off distance.