RESUMO
PURPOSE: This human cadaveric study compares the biomechanical properties of quadriceps tendon repair with suture anchors and the commonly applied transosseous sutures. The hypothesis was that suture anchors provide at least equal results concerning gap formation and ultimate failure load compared with transosseous suture repair. METHODS: Thirty human cadaveric knees underwent tenotomy followed by repair with either 5.5-mm-double-loaded suture anchors [titanium (TA) vs. resorbable hydroxyapatite (HA)] or transpatellar suture tunnels using No. 2 Ultrabraid™ and the Krackow whipstitch. Biomechanical analysis included pretensioning the constructs with 20 N for 30 s and then cyclic loading of 250 cycles between 20 and 100 N at 1 Hz in a servohydraulic testing machine with measurement of elongation. Ultimate failure load analysis and failure mode analysis were performed subsequently. RESULTS: Tendon repairs with suture anchors yielded significantly less gap formation during cyclic loading (20th-250th cycle: TA 1.9 ± 0.1, HA 1.5 ± 0.5, TS 33.3 ± 1.9 mm, p < 0.05) and resisted significantly higher ultimate failure loads (TA 740 ± 204 N, HA 572 ± 67 N, TS 338 ± 60 N, p < 0.05) compared with transosseous sutures. Common failure mode was pull-out of the eyelet within the suture anchor in the HA group and rupture of the suture in the TA and TS group. CONCLUSION: Quadriceps tendon repair with suture anchors yields significantly better biomechanical results than the commonly applied transosseous sutures in this human cadaveric study. These biomechanical findings may change the future clinical treatment for quadriceps tendon ruptures. Randomised controlled clinical trials are desirable for the future. LEVEL OF EVIDENCE: Not applicable, controlled laboratory human cadaveric study.
Assuntos
Músculo Quadríceps/cirurgia , Âncoras de Sutura , Traumatismos dos Tendões/cirurgia , Tendões/cirurgia , Adulto , Fenômenos Biomecânicos , Cadáver , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Músculo Quadríceps/lesões , Músculo Quadríceps/fisiopatologia , Procedimentos de Cirurgia Plástica , Ruptura , Técnicas de Sutura , Traumatismos dos Tendões/fisiopatologia , Tendões/fisiopatologia , Tenotomia , CicatrizaçãoRESUMO
PURPOSE: The success of reconstructions of the posterior cruciate ligament (PCL) mainly depends on the fixation strength of the tendon-bone interface. Reliable data about the mechanical characteristics of PCL fixation techniques are sparse. The aim of this study was to investigate the biomechanical properties of different femoral PCL fixation techniques. METHODS: Fresh human cadaver quadriceps (Q) and hamstring (H) tendons were harvested and fixed into porcine femora with a press-fit fixation suturing the tendon over a bone bridge (group A), a novel implant post-fixation (group B) or an interference screw fixation (group C). Each group consisted of 10 specimens. The constructs were cyclically stretched and eventually loaded until failure. Elongation during cyclic loading, stiffness, failure mode and maximum failure load was evaluated. RESULTS: Elongation during cyclical loading was significantly larger between the 1st and the 20th cycle than between the 20th and the 500th cycle in all groups (p < 0.05). Maximum failure load was 409 ± 71 (336-517) N in group QA, 456 ± 58 (347-510) N in group QB, 548 ± 116 (400-798) N in group QC, 472 ± 114 N (316-676 N) in group HA, 494 ± 98 N (371-668 N) in group HB and 498 ± 87 N (391-687 N) in group HC (significantly higher for QB compared to QA, p < 0.05). CONCLUSION: This is the first study investigating the biomechanical properties of femoral PCL fixations. Implant-free fixation techniques like press-fit or post-fixations are able to withstand equal biomechanical forces compared to interference screw fixation. The novel fixations described in this study can be considered as a reliable alternative for the reconstruction of PCL using either hamstring or quadriceps tendons.
Assuntos
Procedimentos de Cirurgia Plástica/métodos , Ligamento Cruzado Posterior/cirurgia , Tendões/fisiopatologia , Animais , Fenômenos Biomecânicos , Cadáver , Feminino , Fêmur/cirurgia , Humanos , Ligamento Cruzado Posterior/lesões , Técnicas de Sutura , Suínos , Tendões/cirurgiaRESUMO
BACKGROUND: In the context of acute knee dislocations, suture repair of ruptured cruciate ligaments leads to good clinical results in 80% of cases. Disadvantages are low primary stability and subsequently secondary elongation of the sutured ligaments. In the present study, we compared primary stability of suture repair, reinforced by different suture augments, to cruciate ligament reconstruction. OBJECTIVE: The concept of ligament bracing with transosseous suture repair of the cruciate ligaments and additional suture augmentation is biomechanically superior to cruciate ligament reconstruction. MATERIAL AND METHODS: A total of 42 porcine knee joints divided into seven groups were examined. The stability of four different suture/augmentation combinations were compared to cruciate ligament reconstruction with human hamstring tendons. The investigational setup consisted of testing 1000 cycles with 20 N to 154 N load in a.-p. translation and 60° flexion. Elongation and load to failure were measured. RESULTS: Neither reconstruction (3.13 ± 1.65 mm; 362 ± 51 N) nor augmented suture repair (1.89-2.5 mm; 464-624 N) achieved the primary stability of the intact cruciate ligament (0.63 ± 0.34 mm, 1012 ± 91 N). In comparison to ligament reconstruction, all four augmented suture repairs showed minor elongation in the cyclic test and a higher load to failure. The isolated suture repair showed poor results (6.79 ± 4.86 mm, 177 ± 73 N). CONCLUSION: Augmented suture repair provides significantly higher stability compared with isolated suture repair and reconstruction with hamstring tendons. The concept of ligament bracing could be a promising future treatment option in acute knee dislocations. Clinical results remain to be seen.
Assuntos
Reconstrução do Ligamento Cruzado Anterior/instrumentação , Ligamento Cruzado Anterior/fisiopatologia , Ligamento Cruzado Anterior/cirurgia , Técnicas de Sutura/instrumentação , Suturas , Animais , Lesões do Ligamento Cruzado Anterior , Reconstrução do Ligamento Cruzado Anterior/métodos , Desenho de Equipamento , Análise de Falha de Equipamento , Humanos , Técnicas In Vitro , Estresse Mecânico , Suínos , Resistência à TraçãoRESUMO
Reconstruction of critical size bone defects represents a major challenge in orthopaedic surgery. Insufficient angiogenesis is a limiting factor for engraftment of large-scale tissue transplants. Transplantation or stimulation of local mesenchymal stem cells (MSCs) represents a potential solution to enhance angiogenesis. We recently identified angiogenic properties for the Toll-like receptor (TLR) 2/6 agonist MALP-2 and now investigated if MALP-2 could be used to stimulate MSCs in order to promote angiogenesis in vitro and in vivo. Human MSCs from the bone marrow of healthy subjects were isolated, cultured and expanded in vitro and were shown to be positive for mesenchymal stem cells markers as well as for the MALP-2 receptors TLR2 and TLR6. MALP-2 directly enhanced migration but not proliferation of human MSCs. Conditioned medium from MALP-2 stimulated MSCs significantly increased proliferation, migration and tube formation of endothelial cells. Analysis of the conditioned medium from MSCs revealed that MALP-2 stimulation enhanced the secretion of several chemokines and growth factors including vascular endothelial growth factors (VEGF) and granulocyte-macrophage colony-stimulating factor (GM-CSF). Finally, we studied MALP-2 effects on MSCs in a sheep model of tissue engineering in vivo. Therefore, MSCs were isolated from the iliac crest of black head sheep and co-cultivated with MALP-2 ex vivo. Implantation of autologous MSCs within a scaffold cylinder into the M. latissimus dorsi significantly enhanced vessel density of these constructs after 6 months. We here present the first evidence that TLR2/6-dependent stimulation of MSCs promotes angiogenesis in vitro and in vivo offering a novel strategy for therapeutic angiogenesis, e.g., for tissue engineering of bone.
Assuntos
Células-Tronco Mesenquimais/fisiologia , Neovascularização Fisiológica , Comunicação Parácrina , Receptor 2 Toll-Like/metabolismo , Receptor 6 Toll-Like/metabolismo , Animais , Movimento Celular , Proliferação de Células , Quimiocinas/genética , Quimiocinas/metabolismo , Células Endoteliais da Veia Umbilical Humana , Humanos , Peptídeos e Proteínas de Sinalização Intercelular/genética , Peptídeos e Proteínas de Sinalização Intercelular/metabolismo , Lipopeptídeos/farmacologia , Transplante de Células-Tronco Mesenquimais , Células-Tronco Mesenquimais/metabolismo , Músculo Esquelético/fisiologia , Regeneração , Ovinos , Engenharia Tecidual , Receptor 2 Toll-Like/antagonistas & inibidores , Receptor 6 Toll-Like/antagonistas & inibidoresRESUMO
PURPOSE: The objective of this study was to investigate the fibrocartilaginous differentiation occurring in midportion Achilles tendinopathy. METHODS: Tendon samples were retrospectively collected from 23 patients, who had undergone surgery for midportion Achilles tendinopathy resistant to conservative treatment. Based on histological scores, the biopts were subdivided into three categories: a light, moderate and severe histopathological stage. Throughout these stages, immunohistochemical staining was performed against biglycan, aggrecan and collagen type II, components characteristic for fibrocartilage. Staining of these components was evaluated using a semi-quantitative scoring method. RESULTS: The immunohistochemical scores of biglycan and aggrecan were statistically significant between the histopathological stages (P < 0.001). The immunohistochemical scores were positively correlated with the increasing histopathological stages [Spearman's correlation coefficient = 0.93 for biglycan and 0.78 for aggrecan (P < 0.001)]. Staining for collagen type II remained negative throughout these stages. CONCLUSION: Immunohistochemical staining of the fibrocartilaginous components biglycan and aggrecan showed a progressive increase, correlated with a further evolved histopathological stage. This observation gave arguments for an increased differentiation towards fibrocartilaginous components at protein level in midportion Achilles tendinopathy.
Assuntos
Tendão do Calcâneo/patologia , Fibrocartilagem/patologia , Tendinopatia/patologia , Tendão do Calcâneo/química , Tendão do Calcâneo/metabolismo , Adulto , Idoso , Agrecanas/análise , Agrecanas/metabolismo , Biglicano/análise , Biglicano/metabolismo , Diferenciação Celular , Colágeno Tipo II/análise , Colágeno Tipo II/metabolismo , Fibrocartilagem/metabolismo , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Tendinopatia/metabolismo , Tendinopatia/cirurgiaRESUMO
PURPOSE: Double-bundle ACL reconstruction has been demonstrated to be at least as effective as single-bundle reconstruction in terms of restoring knee rotational and translational stability. Until now, the influence on knees with hyperextension has not been evaluated. It was the purpose of this study to evaluate whether double-bundle ACL reconstruction restricts extension in hyperextendable knees. METHODS: Hamstring tendon reconstructions of 10 human cadaveric knees with the ability of hyperextension (age: 48 ± 14 years) were performed as single bundle (SB) on one side and double bundle (DB) on the other side. A surgical navigation system (BrainLab, Germany) was used to assess the kinematics of each knee at the intact and reconstructed state. A difference with regard to the anterior-to-posterior translation (AP) and rotational stability at 30° of knee flexion, 90° of flexion and the hyperextension capability of each specimen was analysed. RESULTS: The difference in AP translation before and after the reconstruction was not significantly different in 30° and 90° of flexion (n.s). Both single- and double-bundle reconstructions restored the preoperative kinematics at 30° and 90° of knee flexion (n.s). The knee extension was 4° ± 1.8° with the intact ACL and 4° ± 1.7° after reconstruction in the SB group (n.s). The knee extension was 5° of hyperextension ± 1.1° with the intact ACL and 0° ± 0.4° after reconstruction in the DB group; the limitation of the extension was significantly larger in this group (p = 0.013). CONCLUSION: Both single- and double-bundle ACL reconstruction techniques are capable of restoring knee anteroposterior and rotational stability. Double-bundle reconstructions significantly reduce knee extension in knees with hyperextension capability. Care must be taken when using double-bundle techniques in patients with knee hyperextension as this procedure may limit the knee extension after double-bundle ACL reconstruction.
Assuntos
Reconstrução do Ligamento Cruzado Anterior/efeitos adversos , Traumatismos do Joelho/fisiopatologia , Articulação do Joelho/fisiopatologia , Amplitude de Movimento Articular , Lesões do Ligamento Cruzado Anterior , Reconstrução do Ligamento Cruzado Anterior/métodos , Humanos , Traumatismos do Joelho/cirurgia , Período Pós-OperatórioRESUMO
PURPOSE: Patellar dislocation usually occurs to the lateral side, leading to ruptures of the medial patellofemoral ligament (MPFL) in about 90 % of all cases. Reliable prognostic factors for the stability of the patellofemoral joint after MPFL surgery and satisfaction of the patient have not been established as yet. METHODS: This multicentric study retrospectively included 40 patients with a mean age of 22.4 ± 8.1 years (range 9-48) from 5 German Trauma Departments with first-time traumatic patellar dislocation and operative treatment. Surgery was limited to soft tissue repairs, and a preoperative magnetic resonance imaging (MRI) was performed in all cases. Evaluation of the MRI included sulcus angle, dysplasia of the trochlea, depth and facet asymmetry of the trochlea, Insall-Salvati index, Tibial tuberosity to trochlear groove (TTTG) distance, and rupture patterns of the MPFL. Patients were interrogated after 2 years about recurrent dislocation, satisfaction, and the Kujala score. RESULTS: Trochlea facet asymmetry was significantly lower in patients with redislocation (23.5 ± 18.8) than in patients without redislocation (43.1 ± 16.5, p = 0.03). Patients with a patellar-based rupture were significantly younger (19.5 ± 7.2 years) than patients without patellar-based rupture (25.4 ± 8.1 years, p < 0.02). Patients with femoral-based ruptures were significantly older (25.7 ± 9.2 years) than patients without femoral-based rupture (19.7 ± 6.1 years, p < 0.02), and had a significantly higher TTTG distance (10.2 ± 6.9 vs. 4.5 ± 5.5, p < 0.02). Patients with incomplete ruptures of the MPFL had a significantly lower Insall-Salvati index (1.2 ± 0.2 vs. 1.4 ± 0.2, p = 0.05). The Kujala score in patients with redislocations was significantly lower (81.0 ± 10.5 points) than in patients without redislocation (91.9 ± 9.2 points, p < 0.02). CONCLUSION: Younger patients more often sustain patellar-based ruptures following first-time traumatic patella dislocation, while older patients more often sustain femoral-based ruptures of the MPFL. Incomplete MPFL ruptures are correlated with lower Insall-Salvati indices. Low trochlear facet asymmetry is correlated with higher rates of redislocation. These results may be of relevance for the operative and postoperative treatment in the future. LEVEL OF EVIDENCE: Prognostic study, Level IV.
Assuntos
Instabilidade Articular/etiologia , Ligamentos Articulares/lesões , Luxação Patelar/cirurgia , Articulação Patelofemoral , Adolescente , Adulto , Criança , Feminino , Humanos , Instabilidade Articular/fisiopatologia , Ligamentos Articulares/fisiopatologia , Ligamentos Articulares/cirurgia , Imageamento por Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Luxação Patelar/complicações , Luxação Patelar/diagnóstico , Luxação Patelar/fisiopatologia , Estudos Retrospectivos , Ruptura , Adulto JovemRESUMO
INTRODUCTION: Patellar dislocation is a common knee injury with mainly lateral dislocations, leading to ruptures of the medial patellofemoral ligament in most of the cases. Even though several prognostic factors for patellofemoral instability have been identified so far, the appropriate therapy for patients with patellar dislocation remains a controversial issue. The purpose of this study was to compare the outcome after conservative or operative treatment in patients after first-time patellar dislocation. PATIENTS AND METHODS: This randomized controlled clinical trial was designed multicentric including patients from six German orthopaedic and trauma departments. Twenty patients with a mean age of 24.6 years with first-time traumatic patella dislocation were included and randomized into either a conservative arm or an operative arm. Plain X-ray images of the knee joint (a.p. and lateral view and tangential view of both patellae) were performed in all cases prior to therapy to exclude osteochondral fragments requiring refixation. An MRI was recommended, but not compulsory. Patients were consulted after 6, 12, and 24 months with a questionnaire including the criteria of the Kujala score, recurrent dislocation, and satisfaction. RESULTS: The mean Kujala score of the conservative vs operative treatment group was 78.6 vs 80.3 after 6 months (p = 0.842), 79.9 vs 88.9 after 12 months (p = 0.165), and 81.3 vs 87.5 after 24 months (p = 0.339). Redislocation rate after 24 months was 37.5 % in the conservative group and 16.7 % in the operative group (p = 0.347). Due to the small number of patients that could be included, no significant difference between the groups could be detected. We see a tendency towards better results after operative treatment. CONCLUSION: Our multicentric prospective randomized controlled trial revealed no significant difference between conservative and operative treatment for patients after first-time traumatic patellar dislocation. However, a tendency towards a better Kujala score and lower redislocation rates for patients with operative treatment was observed. The small number of patients is a limiting factor of the study, leading to results without statistical significance. A meta-analysis including other study's level I data is desirable for the future.
Assuntos
Luxação Patelar/terapia , Adolescente , Adulto , Feminino , Humanos , Masculino , Luxação Patelar/cirurgia , Estudos Prospectivos , Resultado do Tratamento , Adulto JovemRESUMO
Intracondylar deformities after tibial plateau fractures are intra-articular deformities which present within the medial and/or lateral knee compartments. They exist either isolated or in combination with other extra-articular deformities and/or ligament problems. These deformities are complex problems and difficult to treat. While many treatment concepts exist for extra-articular deformities there is limited information available for intra-articular or even intracondylar deformities. Selecting the appropriate procedure for the approach, osteotomy, management of bone defects and cartilage damage is crucial. The authors describe the analysis, planning, treatment and clinical outcome for these rare but difficult problems and present step by step details of the surgical technique.
Assuntos
Instabilidade Articular/etiologia , Instabilidade Articular/cirurgia , Traumatismos do Joelho/complicações , Traumatismos do Joelho/cirurgia , Osteotomia/métodos , Fraturas da Tíbia/complicações , Fraturas da Tíbia/cirurgia , Humanos , Instabilidade Articular/diagnóstico , Traumatismos do Joelho/diagnóstico , Procedimentos de Cirurgia Plástica/métodos , Fraturas da Tíbia/diagnósticoRESUMO
Complex ligament injuries can compromise a knee joint and residual conditions comprise stiffness (arthrofibrosis), instability, cartilage damage leading to osteoarthritis and bone deformity. Accurate diagnosis must address the direction and extent of the instability, the severity of any cartilage lesion and an analysis of the axis and bone deformity as well as important cofactors. Therapeutic options are adhesiolysis, ligament reconstruction, cartilage regeneration and axis correction. As a consequence patients mostly profit from the procedure but there is never a return to the functional level that existed before injury.
Assuntos
Traumatismos do Joelho/diagnóstico , Traumatismos do Joelho/cirurgia , Ligamentos Articulares/lesões , Ligamentos Articulares/cirurgia , Lesões dos Tecidos Moles/diagnóstico , Lesões dos Tecidos Moles/cirurgia , HumanosRESUMO
BACKGROUND: Press-fit fixation of a tendon graft has been advocated in order to achieve tendon to bone healing. HYPOTHESIS: Fixation of a tendon graft with a porous bone scaffold limits bone tunnel enlargement compared with a biodegradable interference screw fixation. METHODS: Between 2005 and 2006, 20 patients (17 men, 3 women) were enrolled in this study for primary reconstruction of the ACL. Patients were randomized to either obtain graft fixation in the tibial tunnel by means of an interference screw (I) or a press-fit fixation with a porous bone cylinder (P). Three months after surgery, a CT scan of the knee was performed and tunnel enlargement was analysed in the coronal and sagittal planes for the proximal, middle and distal thirds of the tunnel. After 6 months, 1 and 2 years, International Knee Documentation Committee (IKDC), Tegner and Lysholm scores of both groups were compared. RESULTS: The bone tunnel enlargement was 106.9±10.9% for group P and 121.9±9.0% for group I (P<0.02) in the AP plane and 102.8±15.2% vs 121.5±10.1% in the coronal plane (P<0.01). IKDC, Tegner, and Lysholm scores improved in both groups from pre- to postoperative assessment without significant differences between the two groups. There was a trend to higher knee stability in group P after 3 months (0.6±1.4 mm vs 1.81±.5 mm, P=0.08). CONCLUSIONS: Both interference screw and a press-fit fixation lead to a high number of good or very good outcomes after ACL reconstruction. Tibial press-fit fixation decreases the amount of proximal bone tunnel enlargement. Press-fit fixation decreases the amount of proximal bone tunnel enlargement and improves bone to tendon contact.
Assuntos
Implantes Absorvíveis , Lesões do Ligamento Cruzado Anterior , Reconstrução do Ligamento Cruzado Anterior/instrumentação , Ligamento Cruzado Anterior/cirurgia , Parafusos Ósseos , Substitutos Ósseos/uso terapêutico , Traumatismos do Joelho/cirurgia , Adulto , Ligamento Cruzado Anterior/diagnóstico por imagem , Reconstrução do Ligamento Cruzado Anterior/métodos , Análise de Falha de Equipamento , Feminino , Humanos , Traumatismos do Joelho/diagnóstico por imagem , Masculino , Estudos Prospectivos , Desenho de Prótese , Radiografia , Resultado do TratamentoRESUMO
BACKGROUND: A secure tibial press fit technique in posterior cruciate ligament reconstructions may be a relevant alternative to common techniques because no hardware is necessary. Up to the present point in time no biomechanical data exist for a tibial press fit posterior cruciate ligament (PCL) reconstruction. This study compares the biomechanical properties of hamstring and quadriceps tendon grafts using a press fit technique with those of an interference screw fixation. METHODS: Quadriceps and hamstring tendons of 20 human cadavers (age 49.2±18.5 years) were used. A press fit fixation with a knot in the semitendinosus tendon (K) and a quadriceps tendon bone block graft (Q) were compared to an interference screw fixation (I) in 27 porcine tibiae. In each group, nine constructs were cyclically stretched and then loaded until failure. Maximum load to failure, stiffness and elongation during failure testing and cyclical loading were investigated. RESULTS: The maximum load to failure was 518±157 N (387-650 N) for the K group, 558±119 N (466-650 N) for the I group and 620±102 N (541-699 N) for the Q group. The stiffness was 55±27 N/mm (18-89 N/mm) for the K group, 117±62 N/mm (69-165 N/mm) for the I group and 65±21 N/mm (49-82 N/mm) for the Q group. The stiffness of the I group was significantly larger (ANOVA on ranks, P=0.01). The elongation during cyclical loading was significantly larger for all groups from the 1st to the 5th cycle compared to the elongation in between the 5th and the 20th cycle (P<0.03). CONCLUSION: All techniques exhibited larger elongation during initial loading. Load to failure and stiffness were significantly different between the fixations. The Q fixation showed equal biomechanical properties compared to a pure tendon fixation (I) with an interference screw. The results of group K were inferior. All three investigated fixation techniques exhibit comparable biomechanical properties. Preconditioning of the constructs is critical. Future randomized, clinical trials have to investigate the biological effectiveness of these fixation techniques.
Assuntos
Procedimentos de Cirurgia Plástica/métodos , Tendões/fisiopatologia , Tendões/transplante , Tíbia/fisiopatologia , Tíbia/cirurgia , Adulto , Idoso , Animais , Cadáver , Módulo de Elasticidade , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Ligamento Cruzado Posterior/lesões , Ligamento Cruzado Posterior/fisiopatologia , Ligamento Cruzado Posterior/cirurgia , Estresse Mecânico , Suínos , Resistência à Tração , Tíbia/lesões , Adulto JovemRESUMO
PURPOSE: A secure tibial press-fit technique in posterior cruciate ligament reconstructions is an interesting technique because no hardware is necessary. For anterior cruciate ligament (ACL) reconstruction, a few press-fit procedures have been published. Up to the present point, no biomechanical data exist for a tibial press-fit posterior cruciate ligament (PCL) reconstruction. The purpose of this study was to characterize a press-fit procedure for PCL reconstruction that is biomechanically equivalent to an interference screw fixation. METHODS: Quadriceps and hamstring tendons of 20 human cadavers (age: 49.2 ± 18.5 years) were used. A press-fit fixation with a knot in the semitendinosus tendon (K) and a quadriceps tendon bone block graft (Q) were compared to an interference screw fixation (I) in 30 porcine femora. In each group, nine constructs were cyclically stretched and then loaded until failure. Maximum load to failure, stiffness, and elongation during failure testing and cyclical loading were investigated. RESULTS: The maximum load to failure was 518 ± 157 N (387-650 N) for the (K) group, 558 ± 119 N (466-650 N) for the (I) group, and 620 ± 102 N (541-699 N) for the (Q) group. The stiffness was 55 ± 27 N/mm (18-89 N/mm) for the (K) group, 117 ± 62 N/mm (69-165 N/mm) for the (I) group, and 65 ± 21 N/mm (49-82 N/mm) for the (Q) group. The stiffness of the (I) group was significantly larger (P = 0.01). The elongation during cyclical loading was significantly larger for all groups from the 1st to the 5th cycle compared to the elongation in between the 5th to the 20th cycle (P < 0.03). CONCLUSION: All techniques exhibited larger elongation during initial loading. Load to failure and stiffness was significantly different between the fixations. The Q fixation showed equal biomechanical properties compared to a pure tendon fixation (I) with an interference screw. All three fixation techniques that were investigated exhibit comparable biomechanical properties. Preconditioning of the constructs is critical. Clinical trials have to investigate the biological effectiveness of these fixation techniques.
Assuntos
Traumatismos do Joelho/cirurgia , Ligamento Cruzado Posterior/lesões , Tendões/transplante , Tenodese/métodos , Animais , Fenômenos Biomecânicos , Feminino , Humanos , Técnicas In Vitro , Masculino , Ligamento Cruzado Posterior/fisiologia , Suínos , Tendões/cirurgia , Tenodese/instrumentação , Resistência à Tração , Suporte de CargaRESUMO
PURPOSE: The purpose of this study was to investigate the influence of continuous perfusion and mechanical stimulation on bone marrow stromal cells seeded on a collagen meniscus implant. METHODS: Bone marrow aspirates from 6 donors were amplified in vitro. 10(6) human BMSC were distributed on a collagen meniscus implant. Scaffolds were cultured under static conditions (control) or placed into a bioreactor system where continuous perfusion (10 ml/min) or perfusion and mechanical stimulation (8 h of 10% cyclic compression at 0.5 Hz) were administered daily. After 24 h, 7 and 14 days, cell proliferation, synthesis of procollagen I and III peptide (PIP, PIIIP), histology, and the equilibrium modulus of the constructs were analyzed. RESULTS: Proliferation demonstrated a significant increase over time in all groups (p < 0.001). PIP synthesis was found to increase from 0.1 ± 0.0 U/ml/g protein after 24 h to 2.0 ± 0.5 (perfusion), 3.8 ± 0.3 (mechanical stimulation), and 1.8 ± 0.2 U/ml/g protein (static control, lower than perfusion and mechanical stimulation, p < 0.05). These differences were also evident after 2 weeks (2.7 ± 0.3, 4.0 ± 0.6, and 1.8 ± 0.2 U/ml/g protein, p < 0.01); PIIIP synthesis was found to increase from 0.1 ± 0.0 U/ml/g protein after 24 h to 2.9 ± 0.7 (perfusion), 3.1 ± 0.9 (mechanical stimulation), and 1.6 ± 0.3 U/ml/g protein (controls) after 1 week and remained significantly elevated under the influence of perfusion and mechanical stimulation (p < 0.01) after 2 weeks. Mechanical stimulation increased the equilibrium modulus more than static culture and perfusion after 2 weeks (24.7 ± 7.6; 12.3 ± 3.7; 15.4 ± 2.6 kPa; p < 0.02). CONCLUSION: Biomechanical stimulation and perfusion have impact on collagen scaffolds seeded with BMSCs. Cell proliferation can be enhanced using continuous perfusion and differentiation is fostered by mechanical stimulation.
Assuntos
Colágeno , Meniscos Tibiais , Perfusão , Engenharia Tecidual , Alicerces Teciduais , Fenômenos Biomecânicos , Reatores Biológicos , Células da Medula Óssea/fisiologia , Proliferação de Células , Células Cultivadas , Colágeno/metabolismo , Humanos , Meniscos Tibiais/citologia , Meniscos Tibiais/metabolismo , Meniscos Tibiais/fisiologia , Pressão , Pró-Colágeno/metabolismo , Radioimunoensaio , Células Estromais/fisiologiaRESUMO
INTRODUCTION: There is a large variety of ruptures of tendons and ligaments in trauma surgery. Reliable data about the most appropriate suture technique and suture material for ruptured tendons are sparse. This human cadaveric study compares the biomechanical properties of three suture materials and three suture techniques for semitendinosus tendon repair. METHOD: Sixty-three human cadaver hamstring tendons underwent tenotomy and repair with either Baseball suture, Kessler suture, or a novel "Hannover" suture, using either PDS 2-0, Ethibond 2-0, or Fiberwire 2-0. Biomechanical analysis included pretensioning the constructs with 2 N for 50 s, then cyclic loading of 500 cycles between 2 and 15 N at 1 Hz in a servohydraulic testing machine with measurement of elongation. After this, ultimate failure load and failure mode analysis was performed. RESULTS: Ruptures repaired by Fiberwire™ as suture material and the Baseball suture technique were able to withstand significantly higher maximum failure loads (72.8 ± 22.0 N, p < 0.001) than the Kessler suture and the Hannover suture, while ruptures repaired by Fiberwire and the Kessler suture technique showed the lowest elongation after cyclic loading (14.6 ± 3.8 mm, p = 0.15). CONCLUSION: These findings may be of relevance for the future clinical treatment of tendon ruptures. Further in vivo clinical application studies are desirable for the future.
Assuntos
Técnicas de Sutura , Suturas , Traumatismos dos Tendões/cirurgia , Adulto , Idoso , Fenômenos Biomecânicos , Humanos , Técnicas In Vitro , Pessoa de Meia-Idade , Ruptura , Adulto JovemRESUMO
Patellar dislocation is a common knee injury with mainly lateral dislocations, leading to ruptures of the medial patellofemoral ligament in most of the cases. Reliable data and prognostic factors for stability of the patellofemoral joint and satisfaction of the patient after either conservative or operative treatment have not been established yet. Until now, there are no randomized controlled trials for recurrent patellar dislocation at all. As a synopsis of the randomized controlled trials about first-time patellar dislocation, no significant difference between operative and conservative management is evident. This applies to both children and adolescents as well as to adults. There is a clear tendency towards first-line conservative therapy after traumatic patellar dislocation. Operative treatment is only required in case of accompanying injuries like osteochondral fractures or in case of recurrent dislocations. Further prospective randomized controlled trials with standardized operative and conservative treatment and patient cohorts of sufficient size are necessary in the future.
Assuntos
Medicina Baseada em Evidências , Instabilidade Articular/diagnóstico , Instabilidade Articular/cirurgia , Luxação Patelar/diagnóstico , Luxação Patelar/cirurgia , Articulação Patelofemoral/cirurgia , Adolescente , Adulto , Humanos , Resultado do Tratamento , Adulto JovemRESUMO
BACKGROUND: Press-fit fixation of hamstring tendon autografts for anterior cruciate ligament reconstruction is an interesting technique because no hardware is necessary. This study compares the biomechanical properties of press-fit fixations to an interference screw fixation. METHODS: Twenty-eight human cadaveric knees were used for hamstring tendon explantation. An additional bone block was harvested from the tibia. We used 28 porcine femora for graft fixation. Constructs were cyclically stretched and then loaded until failure. Maximum load to failure, stiffness and elongation during failure testing and cyclic loading were investigated. RESULTS: The maximum load to failure was 970±83 N for the press-fit tape fixation (T), 572±151 N for the bone bridge fixation (TS), 544±109 N for the interference screw fixation (I), 402±77 N for the press-fit suture fixation (S) and 290±74 N for the bone block fixation technique (F). The T fixation had a significantly better maximum load to failure compared to all other techniques (p<0.001). CONCLUSION: This study demonstrates that a tibial press-fit technique which uses an additional bone block has better maximum load to failure results compared to a simple interference screw fixation.
Assuntos
Lesões do Ligamento Cruzado Anterior , Reconstrução do Ligamento Cruzado Anterior/métodos , Ligamento Cruzado Anterior/fisiopatologia , Tendões/fisiopatologia , Tendões/transplante , Adolescente , Adulto , Idoso , Ligamento Cruzado Anterior/cirurgia , Reconstrução do Ligamento Cruzado Anterior/instrumentação , Cadáver , Módulo de Elasticidade , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Pressão , Resistência à Tração , Resultado do Tratamento , Adulto JovemRESUMO
We present a literature review about implant removal after operative extremity and spine fracture treatment. The indication for implant removal procedures has become less frequent in recent years, but is still more common in Europe than for example in North America. The time required to perform a implant removal can easily exceed the planned amount. Implant removal can result in significant complications like soft tissue damage, fractures, infections, and other problems. Not only because of these problems, the decision on whether or not to remove the implant should be made with great care. Therefore good communication with the patient and thorough information about risks and benefits are essential.
Assuntos
Remoção de Dispositivo/efeitos adversos , Remoção de Dispositivo/métodos , Fixação Interna de Fraturas/efeitos adversos , Fraturas Ósseas/cirurgia , Próteses e Implantes/efeitos adversos , Infecções Relacionadas à Prótese/etiologia , Infecções Relacionadas à Prótese/prevenção & controle , Fixação Interna de Fraturas/instrumentação , HumanosRESUMO
PURPOSE: Press-fit fixation of patellar tendon bone anterior cruciate ligament autografts is an interesting technique because no hardware is necessary. To date, no biomechanical data exist describing an implant-free double-bundle press-fit procedure. The purpose of this study was to characterize the biomechanical properties of three double-bundle press-fit fixations. METHODS: In a controlled laboratory study, the patellar-, quadriceps- and hamstring tendons of 10 human cadavers (age: 49.2 ± 18.5 years) were used. An inside out press-fit fixation with a knot in the semitendinosus and gracilis tendons (SG) combined with an additional bone block, with two quadriceps tendon bone block grafts (QU) was compared with press-fit fixation of two bone patellar tendon bone block (PT) grafts in 30 porcine femora. Constructs were cyclically stretched and then loaded until failure. Maximum load to failure, stiffness and elongation during failure testing and cyclical loading were investigated. RESULTS: The maximum load to failure was 703 ± 136 N for SG fixation, 632 ± 130 N for QU and 656 ± 127 N for PT fixation. Stiffness of the constructs averaged 138 ± 26 N/mm for SG, 159 ± 74 N/mm for QU, and 154 ± 50 N/mm for PT fixation. Elongation during initial cyclical loading was 1.2 ± 1.4 mm for SG, 2.0 ± 1.4 mm for QU, and 1.0 ± 0.6 mm for PT (significantly larger for PT and QU between the first 5 cycles compared with cycles 15-20th, P < 0.01). CONCLUSION: All investigated double-bundle fixation techniques were equal in terms of maximum load to failure, stiffness, and elongation. Unlike with single-bundle press-fit fixation techniques that have been published, no difference was observed between pure tendon combined with an additional bone block and tendon bone grafts. All techniques exhibited larger elongation during initial cyclical loading. All three press-fit fixation techniques that were investigated exhibit comparable biomechanical properties. Preconditioning of the constructs is critical.