Effects of δ-aminolevulinic acid and vitamin C supplementation on iron status, production performance, blood characteristics and egg quality of laying hens.

An experiment was conducted to evaluate the effects of laying hen diets supplemented with δ-aminolevulinic acid (ALA) and vitamin C (VC) on productive performance, iron status and egg quality. A total of 252 Hy-line brown commercial laying hens were fed two levels of VC (0 and 500 mg/kg) and three levels of ALA (0, 5 and 10 mg/kg) in a 2 × 3 factorial arrangement from 57 to 63 weeks of age. Each treatment contained seven replicates with six hens in each replicate. Supplementation of the diet with both ALA and VC resulted in a significant increase in egg production during 4-6 weeks (p < 0.05). The haematocrit (HCT), total iron-binding capacity (TIBC), white blood cell (WBC), total protein and albumin concentrations were not affected by the dietary treatments. However, ALA and interactive effect of ALA and VC were observed to increase the RBC, haemoglobin and serum iron concentrations (p < 0.05). In addition, inclusion of both ALA and VC increased blood lymphocyte percentage in relation to hens not supplemented with ALA (p < 0.05). Moreover, single effect of ALA or VC improved (p < 0.05) the yolk colour unit and albumin height from 3 to 6 weeks. The egg shell colour was increased (p < 0.05) by the effect of ALA. The addition of VC also led to an increase (p < 0.05) of the Haugh unit at the end of the experiment. However, neither ALA, nor VC exerted an effect on egg weight, egg shell breaking strength or egg shell thickness. The results demonstrate that dietary ALA and VC supplementation can improve the iron status and egg quality of laying hens.

Effects of chitooligosaccharide supplementation on performance, blood characteristics, relative organ weight, and meat quality in broiler chickens.

This study was conducted to evaluate the effects of dietary supplementation with chitooligosaccharide (COS) on growth performance, blood characteristics, relative organ weight, and meat quality in broilers. A total of 480 broilers with an average initial BW of 45.04 g per chick were randomly allocated into 1 of the following 4 dietary treatments (20 broilers per pen with 6 pens per treatment): 1) CON (basal diet), 2) ANT (basal diet + 44 mg/kg of avilamycin), 3) COS0.2 (basal diet + 14 g/kg of COS), 4) COS0.4 (basal diet + 28 g/kg of COS). The experiment lasted for 5 wk and avilamycin was administered from d 0 to 21. Growth performance was measured on d 0, 21, and 35, and all other response criteria were measured on d 35. No change in feed conversion (G:F) was observed in response to any of the treatments throughout the experimental period (P > 0.05). However, BW gain and feed intake were greater (P < 0.05) in broilers provided with feed supplemented with COS than in those in the control group. In addition, broilers had significantly greater (P < 0.05) red blood cell and high-density lipoprotein cholesterol concentrations when they were provided with the COS0.4 diet, whereas the triglyceride concentration was lower (P < 0.05) in broilers in the COS0.2 treatment group. No other blood characteristics were affected by the treatments. Additionally, as the dietary COS concentration increased, the liver weight increased (P < 0.05). Conversely, as the concentrations of dietary COS increased, abdominal fat decreased (P < 0.05). Furthermore, meat yellowness decreased (P < 0.05) as the concentration of COS increased. Finally, the breast meat and abdominal fat of birds provided with feed supplemented with COS had a lower (P < 0.05) saturated fatty acid concentration but a greater concentration of total monounsaturated fatty acids (P < 0.05) than that of birds in the control. In conclusion, COS can improve the performance and breast meat quality of broilers while increasing the red blood cell and high-density lipoprotein cholesterol concentrations in blood. In addition, COS can induce a decrease in abdominal fat and improve meat quality.

Antioxidative phenolic compounds from the roots of Rhodiola sachalinensis A. Bor.

The acetone extract of the roots of Rhodiola sachalinensis has furnished six phenolic compounds which exhibited significant scavenging effects against DPPH free radical. The structures of these compounds were identified and determined as gallic acid (1), (-)-epigallocatechin 3-O-gallate (2), kaempferol (3), kaempferol 7-O-alpha-L-rhamnopyranoside (4), herbacetin 7-O-alpha-L-rhamnopyranoside, (5) and rhodiolinin (6) by physico-chemical and spectral evidences.

Effect of dietary fermented garlic by Weissella koreensis powder on growth performance, blood characteristics, and immune response of growing pigs challenged with Escherichia coli lipopolysaccharide.

Two experiments were conducted to investigate the effect of fermented garlic by Weissella koreensis powder (WKG) on pig growth performance and immune responses after an Escherichia coli lipopolysaccharide (LPS) challenge. In Exp. 1, 120 growing barrows (23.5 ± 0.5 kg of BW and 56 d of age) were used in a 35-d experiment to determine the optimal amounts of WKG. Pigs were randomly allotted to 1 of 5 treatments with 6 replicate pens and 4 pigs per pen. Dietary treatments included 1) NC (negative control; basal diet without antibiotics), 2) PC (positive control; basal diet + 1 g of tylosin/kg), 3) WKG1 (basal diet + 1 g of WKG/kg), 4) WKG2 (basal diet + 2 g of WKG/kg), and 5) basal diet + 4 g of WKG/kg. At the end of the feeding period, 12 pigs each were selected from the NC and WKG2 treatment groups, and 6 pigs were injected with LPS (50 µg/kg of BW) and the other 6 pigs with an equivalent amount of sterile saline, resulting in a 2 × 2 factorial arrangement of treatments. Blood samples and rectal temperature data were collected at 0, 2, 4, 6, 8, and 12 h after challenge. The ADG of pigs fed WKG- and antibiotic-supplemented diets was greater (P<0.05) than NC from d 14 to 35 and the overall phase, but no dosage-dependent effects were observed. At the end of the experiment, the fecal E. coli count was linearly reduced by the increasing amounts of WKG at d 35 (P=0.01). Challenge with LPS increased white blood cell counts at 6 and 8 h (P<0.01) and depressed lymphocyte concentration at 4, 8, and 12 h (P<0.01). During challenge, LPS injection increased rectal temperature at 2, 4, 6, and 8 h postchallenge (P<0.05), and WKG2 alleviated (P<0.05) the increase in the temperature at 2 h postchallenge. The LPS injection increased plasma tumor necrosis factor-α and IGF-1 concentrations at 2, 4, 6, 8, and 12 h (P<0.01), whereas an alleviating effect of WKG was observed at 4, 6, and 8 h after LPS challenge (P<0.05). At 2, 4, and 6 h postchallenge, concentration of cluster of differentiation-antigen-4-positive cells and cluster of differentiation-antigen-8-positive cells (CD4(+) and CD8(+), respectively) increased in the LPS treatments (P<0.05), and the WKG2 boosted this effect (P<0.05). In conclusion, dietary supplementation of WKG2 in growing pigs can improve ADG and have a beneficial effect on the immune response during an inflammatory challenge.

Effects of phenyllactic acid on growth performance, nutrient digestibility, microbial shedding, and blood profile in pigs.

Ninety weanling pigs in Exp. 1 (6.27 +/- 0.73 kg; 21 d of age) and 96 growing pigs in Exp. 2 (21.73 kg +/- 1.29 kg; 56 d of age) were used in two 42-d experiments to evaluate the effect of phenyllactic acid (PLA) on growth performance, apparent total tract digestibility (ATTD) of DM and N, fecal pH value, microbial shedding, and blood profiles. In Exp. 1, the 3 dietary treatments were 1) negative control (NC), 2) positive control (PC), NC + antibiotics, and 3) PLA, NC + 0.5% PLA. In Exp. 2, dietary treatments were 1) control diet (CON), 2) PLA-0.1, CON + 0.1% PLA, 3) PLA-0.2, CON + 0.2% PLA, and 4) PLA-0.3, CON + 0.3% PLA. In Exp. 1, pigs fed the PC and PLA diets had greater ADFI during the overall period (P < 0.05) and tended to have greater ADG and G:F from d 7 to 21 (P < 0.10) than those fed the NC diet. The ATTD of DM was greatest in pigs fed the PLA diet on d 20 and 41, and N digestibility on d 20 was greater in pigs fed the PLA diet (P < 0.05) than those fed the NC diet. The numbers of white blood cell and lymphocyte concentrations on d 42 were increased (P < 0.05) by the inclusion of antibiotics and PLA in the diet. In Exp. 2, G:F tended to increase when PLA was added (quadratic, P < 0.10). The ATTD of DM did not differ among treatments, but there was a tendency (quadratic, P < 0.10) for N digestibility to increase as PLA levels increased. The lymphocyte percentage on d 42 increased linearly as dietary PLA increased (P < 0.05). Additionally, the white blood cell counts on d 42 tended to increase as PLA levels increased (P < 0.10). In both experiments, there was no effect of treatment on the fecal pH or presence of Lactobacillus, but the number of Escherichia coli in feces on d 41 decreased in response to the addition of PLA [P < 0.05 and 0.001 (linear) in Exp. 1 and 2, respectively]. In conclusion, PLA can decrease the number of E. coli, and this novel dietary acid may have potential to stimulate the immune system for both weanling and growing pigs. Thus, it could be a good candidate as an alternative to antibiotics in pig diets.

Characteristics of the interaction of calcium with casein submicelles as determined by analytical affinity chromatography.

Interaction of calcium with casein submicelles was investigated in CaCl2 and calcium phosphate buffers and with synthetic milk salt solutions using the technique of analytical affinity chromatography. Micelles that had been prepared by size exclusion chromatography with glycerolpropyl controlled-pore glass from fresh raw skim milk that had never been cooled, were dialyzed at room temperature against calcium-free imidazole buffer, pH 6.7. Resulting submicelles were covalently immobilized on succinamidopropyl controlled-pore glass (300-nm pore size). Using 45Ca to monitor the elution retardation, the affinity of free Ca2+ and calcium salt species was determined at temperatures of 20 to 40 degrees C and pH 6.0 to 7.5. Increasing the pH in this range or increasing the temperature strengthened the binding of calcium to submicelles, similar to previous observations with individual caseins. However, the enthalpy change obtained from the temperature dependence was considerably greater than that reported for alpha s1- and beta-caseins. Furthermore, the elution profiles for 45Ca in milk salt solutions were decidedly different from those in CaCl2 or calcium phosphate buffers and the affinities were also greater. For example, at pH 6.7 and 30 degrees C the average dissociation constant for the submicelle-calcium complex is 0.074 mM for CaCl2 and calcium phosphate buffers, vs 0.016 mM for the milk salt solution. The asymmetric frontal boundaries and higher average affinities observed with milk salts may be due to binding of calcium salts with greater affinity in addition to the binding of free Ca2+ in these solutions.

Protein enrichment of sweet potato residue by solid-state cultivation with mono- and co-cultures of amylolytic fungi.

The degree of protein enrichment of sweet potato residue by different amylolytic moulds in solid-state cultivation was much greater than that obtained using amylolytic yeasts. The optimum initial moisture content for protein enrichment was about 65% (w/w). Adding nitrogen sources to the culture twice (at the start of the incubation and after 24 h) considerably improved the final protein content. A co-culture of amylolytic mycelial fungi yielded a product with 32% (w/w) crude protein after 4 days' incubation at 30°C. In a column reactor, the highest temperatures reached were 42°C and 40°C and the minimum O2 concentrations were 1.5% and 2.5% of full saturation in the central and bottom layers, respectively.

Caspase-cleaved TRAF1 negatively regulates the antiapoptotic signals of TRAF2 during TNF-induced cell death.

Tumor necrosis factor (TNF) signaling leads to pleiotropic responses in a wide range of cell types, in part by activating antiapoptotic and proapoptotic pathways. Previous studies have suggested that TNF receptor-associated factor (TRAF) 2 can mediate crucial antiapoptotic signals during TNF stimulation. However, it is unclear how the antiapoptotic signals via TRAF2 in TNF-R1 signaling is regulated. Here we show that TRAF1 is cleaved by caspase-8 into two fragments during apoptosis induced by TNF. Overexpression of the C-terminal cleavage product, TRAF1-c, increased TNF-induced cell death of hybridoma T cells. Importantly, we demonstrate that the cleavage product of TRAF1 coimmunoprecipitates with TRAF2 that is released from the TNF-R1 complex in response to prolonged TNF treatment. These results indicate that caspase-dependent cleavage of TRAF1 generates TRAF1-c fragments that are able to bind TRAF2, and then sequester TRAF2 from the TNF-R1 complex, rendering cells, at least in part, sensitive to TNF.