RESUMO
Chemical communication is ubiquitous. The identification of conserved structural elements in visual and acoustic communication is well established, but comparable information on chemical communication displays (CCDs) is lacking. We assessed the phenotypic integration of CCDs in a meta-analysis to characterize patterns of covariation in CCDs and identified functional or biosynthetically constrained modules. Poorly integrated plant CCDs (i.e. low covariation between scent compounds) support the notion that plants often utilize one or few key compounds to repel antagonists or to attract pollinators and enemies of herbivores. Animal CCDs (mostly insect pheromones) were usually more integrated than those of plants (i.e. stronger covariation), suggesting that animals communicate via fixed proportions among compounds. Both plant and animal CCDs were composed of modules, which are groups of strongly covarying compounds. Biosynthetic similarity of compounds revealed biosynthetic constraints in the covariation patterns of plant CCDs. We provide a novel perspective on chemical communication and a basis for future investigations on structural properties of CCDs. This will facilitate identifying modules and biosynthetic constraints that may affect the outcome of selection and thus provide a predictive framework for evolutionary trajectories of CCDs in plants and animals.
Assuntos
Evolução Biológica , Vias Biossintéticas , Animais , Fenótipo , Compostos Orgânicos Voláteis/metabolismoRESUMO
Stingless bees have evolved several ways to share contested resources to ensure the coexistence between different species. Partamona orizabaensis quickly exploits food sources by fast and direct recruitment that does not rely on scent marks deposited on substrates. In this study we show that the flight activity of P. orizabaensis is influenced by weather conditions, with higher activity during periods of colder temperatures, higher relative humidity and even during rainfall. We showed that the outcome of aggression experiments between the non-aggressive species P. orizabaensis and its aggressive competitor Trigona fuscipennis is influenced by the number of bees that arrive early after food source discovery. Therefore, the increased activity during less favorable weather conditions and the fast recruitment of nestmates following the discovery of a food source, as observed for P. orizabaensis, may be adaptations that evolved to coexist even with more aggressive and dominant species of stingless bees, with which P. orizabaensis has to compete for resources.
Assuntos
Agressão/fisiologia , Clima , Comportamento Competitivo/fisiologia , Comportamento Alimentar/fisiologia , Voo Animal/fisiologia , Comunicação Animal , Animais , AbelhasRESUMO
Foragers of several stingless bee species deposit attractive scent marks on solid substrates to precisely recruit nestmates to food. Interestingly, Partamona workers quickly recruit large numbers of nest mates to resources, likely even without the deposition of attractive scent marks. However, systematic studies of the recruitment system of these bees are lacking. We now studied the recruitment behavior of P. orizabaensis. Our findings show that foragers of this species can recruit large numbers of nestmates to food sources at a particular location. The precise nestmate recruitment does not rely on attractive scent marks deposited on substrates. We never observed any scent marking behavior and feeders baited with labial or mandibular gland extracts were not attractive for the bees. Chemical analyses showed that the foragers' labial gland secretions exclusively contain long chain hydrocarbons, which render their role in recruitment communication unlikely. Whether mandibular gland secretions, which contain esters and alcohols that are known as attractive pheromones in other bee species, are used to guide recruits toward food during flight, remains elusive. We conclude that Partamona's quick recruitment system that does not rely on conspicuous scent marks has evolved as a strategy against competition with sympatrically occurring and more aggressive bee species.
Assuntos
Comunicação Animal , Abelhas/fisiologia , Ecossistema , Comportamento Alimentar/fisiologia , Feromônios/fisiologia , AnimaisRESUMO
Stingless bees (Hymenoptera, Apidae, Meliponini) are by far the largest group of eusocial bees on Earth. Due to the diversity of evolutionary responses to specific ecological challenges, the Meliponini are well suited for comparative studies of the various adaptations to the environment found in highly eusocial bees. Of particular interest are the physiological mechanisms underlying the sophisticated cooperative and collective actions of entire colonies, which form the basis of the ecological success of the different bee species under the particular conditions prevailing in their respective environment. The present Special Issue of the Journal of Comparative Physiology A provides a sample of the exciting diversity of sensorial and behavioral adaptations in stingless bees, particularly concerning (1) the sensory bases for foraging, (2) chemical communication, and (3) the behavioral ecology of foraging.
Assuntos
Abelhas/fisiologia , Comportamento Animal/fisiologia , Sensação/fisiologia , Animais , Meio AmbienteRESUMO
Bumble bees are of major importance, ecologically and economically as pollinators in cool and temperate biomes and as model organisms for scientific research. Chemical signals and cues have been shown to play an outstanding role in intraspecific and interspecific communication systems within and outside of a bumble bee colony. In the present review we compile and critically assess the literature on the chemical ecology of bumble bees, including cuckoo bumble bees. The development of new and more sensitive analytical tools and improvements in sociogenetic methods significantly enhanced our knowledge about chemical compounds that mediate the regulation of reproduction in the social phase of colony development, about the interactions between host bumble bees and their social parasites, about pheromones involved in mating behavior, as well as about the importance of signals, cues and context-dependent learning in foraging behavior. Our review intends to stimulate new studies on the many unresolved questions concerning the chemical ecology of these fascinating insects.
Assuntos
Abelhas/fisiologia , Abelhas/parasitologia , Animais , Comportamento Apetitivo , Comportamento Sexual Animal , Comportamento SocialRESUMO
Among the large number of exocrine glands described in bees, the tarsal glands were thought to be the source of footprint scent marks. However, recent studies showed that the compounds used for marking by stingless bees are secreted by leg tendon instead of tarsal glands. Here, we report on the structure of leg tendon glands in males of Bombus terrestris, together with a description of the chemical composition of their secretions and respective changes of both during the males' lives. The ultrastructure of leg tendon glands shows that the secretory cells are located in three independent regions, separated from each other by unmodified epidermal cells: in the femur, tibia, and basitarsus. Due to the common site of secretion release, the organ is considered a single secretory gland. The secretion of the leg tendon glands of B. terrestris males differs in its composition from those of workers and queens, in particular by (1) having larger proportions of compounds with longer chain lengths, which we identified as wax esters; and (2) by the lack of certain hydrocarbons (especially long chain dienes). Other differences consist in the distribution of double bond positions in the unsaturated hydrocarbons that are predominantly located at position 9 in males but distributed at seven to nine different positions in the female castes. Double bond positions may change chemical and physical properties of a molecule, which can be recognized by the insects and, thus, may serve to convey specific information. The function of male-specific compounds identified from their tendon glands remains elusive, but several possibilities are discussed.
Assuntos
Abelhas/fisiologia , Abelhas/ultraestrutura , Envelhecimento , Animais , Abelhas/anatomia & histologia , Abelhas/química , Secreções Corporais/química , Glândulas Exócrinas/ultraestrutura , Extremidade Inferior/anatomia & histologia , Masculino , Microscopia Eletrônica de VarreduraRESUMO
Foragers of several species of stingless bees (Hymenoptera, Apidae and Meliponini) deposit pheromone marks in the vegetation to guide nestmates to new food sources. These pheromones are produced in the labial glands and are nest and species specific. Thus, an important question is how recruited foragers recognize their nestmates' pheromone in the field. We tested whether naïve workers learn a specific trail pheromone composition while being recruited by nestmates inside the hive in the species Scaptotrigona pectoralis. We installed artificial scent trails branching off from trails deposited by recruiting foragers and registered whether newly recruited bees follow these trails. The artificial trails were baited with trail pheromones of workers collected from foreign S. pectoralis colonies. When the same foreign trail pheromone was presented inside the experimental hives while recruitment took place a significant higher number of bees followed the artificial trails than in experiments without intranidal presentation. Our results demonstrate that recruits of S. pectoralis can learn the composition of specific trail pheromone bouquets inside the nest and subsequently follow this pheromone in the field. We, therefore, suggest that trail pheromone recognition in S. pectoralis is based on a flexible learning process rather than being a genetically fixed behaviour.
Assuntos
Abelhas/fisiologia , Comportamento Exploratório/fisiologia , Comportamento Alimentar/fisiologia , Aprendizagem/fisiologia , Atrativos Sexuais/fisiologia , Olfato/fisiologia , Animais , Comportamento Animal/fisiologia , Comportamento Alimentar/psicologia , Comportamento SocialRESUMO
Stingless bees, like honeybees, live in highly organized, perennial colonies. Their eusocial way of life, which includes division of labor, implies that only a fraction of the workers leave the nest to forage for food. To ensure a sufficient food supply for all colony members, stingless bees have evolved different mechanisms to recruit workers to foraging or even to communicate the location of particular food sites. In some species, foragers deposit pheromone marks between food sources and their nest, which are used by recruited workers to locate the food. To date, pheromone compounds have only been described for 3 species. We have identified the trail pheromone of a further species by means of chemical and electrophysiological analyses and with bioassays testing natural gland extracts and synthetic compounds. The pheromone is a blend of wax type and terpene esters. The relative proportions of the single components showed significant differences in the pheromones of foragers form 3 different colonies. This is the first report on a trail pheromone comprised of esters of 2 different biogenetic origins proving variability of the system. Pheromone specificity may serve to avoid confusions between the trails deposited by foragers of different nests and, thus, to decrease competition at food sources.
Assuntos
Abelhas/química , Abelhas/fisiologia , Feromônios/química , Animais , Cromatografia Gasosa , Especificidade da EspécieRESUMO
The ability to learn food odors inside the nest and to associate them with food sources in the field is of essential importance for the recruitment of nestmates in social bees. We investigated odor learning by workers within the hive and the influence of these odors on their food choice in the field in the stingless bee Scaptotrigona pectoralis. During the experiments, recruited bees had to choose between two feeders, one with an odor that was present inside the nest during the recruitment process, and one with an unknown odor. In all experiments with different odor combinations (linalool/phenylacetaldehyde, geraniol/eugenol) a significant majority of bees visited the feeder with the odor they had experienced in their nest (chi (2)-tests; p < 0.05). By contrast, the bees showed no preference for one of two feeders when they were either baited with the same odor (linalool) or contained no odor. Our results clearly show that naïve workers of S. pectoralis can learn the odor of a food source during the recruitment process from the nest atmosphere and that their subsequent food search in the field is influenced by the learned odor.
Assuntos
Ração Animal , Abelhas/fisiologia , Comportamento Alimentar/fisiologia , Aprendizagem/fisiologia , Comportamento de Nidação , Odorantes , Comportamento Social , Animais , Abelhas/anatomia & histologia , Feminino , Abrigo para Animais , SacaroseRESUMO
Reproductive division of labor in advanced eusocial honey bees and stingless bees is based on the ability of totipotent female larvae to develop into either workers or queens. In nearly all species, caste is determined by larval nutrition. However, the mechanism that triggers queen development in Melipona bees is still unresolved. Several hypotheses have been proposed, ranging from the proximate (a genetic determination of caste development) to the ultimate (a model in which larvae have complete control over their own caste fate). Here, we showed that the addition of geraniol, the main compound in labial gland secretions of nurse workers, to the larval food significantly increases the number of larvae that develop into queens. Interestingly, the proportion of queens in treated brood exactly matched the value (25%) predicted by the two-locus, two-allele model of genetic queen determination, in which only females that are heterozygous at both loci are capable of developing into queens. We conclude that labial gland secretions, added to the food of some cells by nurse bees, trigger queen development, provided that the larvae are genetically predisposed towards this developmental pathway. In Melipona beecheii, geraniol acts as a primer pheromone representing the first caste determination substance identified to date.
Assuntos
Abelhas/crescimento & desenvolvimento , Terpenos/química , Monoterpenos Acíclicos , Animais , Cromatografia Gasosa , Feminino , Larva/crescimento & desenvolvimento , Glândulas Salivares/química , Predomínio Social , Terpenos/farmacologiaRESUMO
Stingless bees of the species Trigona spinipes (Fabricius 1793) use their saliva to lay scent trails communicating the location of profitable food sources. Extracts of the cephalic labial glands of the salivary system (not the mandibular glands, however) contain a large amount (approx. 74%) of octyl octanoate. This ester is also found on the scent-marked substrates at the feeding site. We demonstrate octyl octanoate to be a single compound pheromone which induces full trail following behaviour. The identification of the trail pheromone in this widely distributed bee makes it an ideal organism for studying the mechanism of trail following in a day flying insect.
Assuntos
Comunicação Animal , Abelhas/fisiologia , Feromônios/fisiologia , Saliva/fisiologia , Animais , Abelhas/anatomia & histologia , Abelhas/metabolismo , Caprilatos/metabolismo , Feromônios/química , Saliva/químicaRESUMO
Since the seminal work of Lindauer and Kerr (1958), many stingless bees have been known to effectively recruit nestmates to food sources. Recent research clarified properties of several signals and cues used by stingless bees when exploiting food sources. Thus, the main source of the trail pheromone in Trigona are the labial, not however the mandibular glands. In T. recursa and T. spinipes, the first stingless bee trail pheromones were identified as hexyl decanoate and octyl decanoate, respectively. The attractant footprints left by foragers at the food source are secreted by glandular epithelia of the claw retractor tendon, not however by the tarsal gland. Regarding intranidal communication, the correlation between a forager's jostling rate and recruitment success stresses the importance of agitated running and jostling. There is no evidence for a "dance" indicating food source location, however, whereas the jostling rate depends on food quality. Thoracic vibrations, another intranidal signal well known in Melipona, were analyzed using modern technology and distinguishing substrate vibrations from airborne sound. Quantitative data now permit estimates of signal and potential communication ranges. Airflow jets as described for the honeybee were not found, and thoracic vibrations do not "symbolically" encode visually measured distance in M. seminigra.
Assuntos
Comunicação Animal , Abelhas/fisiologia , Sinais (Psicologia) , Comportamento Alimentar , Animais , Feromônios/metabolismo , Glândulas Odoríferas/metabolismo , Comportamento Social , Tórax/fisiologia , Vibração , Vocalização AnimalRESUMO
An element common to the recruitment communication of eusocial bees (honey bees, stingless bees and bumble bees) are pulsed thorax vibrations generated by successful foragers within the nest. In stingless bees, foragers vibrate during the unloading of the collected food. In the present study on Melipona seminigra we demonstrate that during trophallactic contacts, the food receivers are directly vibrated by the foragers. As a consequence, both the temporal structure and the main frequency component of the forager's vibrations are directly passed on to the receiver. The vibrations are attenuated by about 17 dB on their way from the forager's thorax (velocity amplitude of the vibrations: approximately 70 mm/s) to the receiver's thorax (approximately 10 mm/s), the main amount of attenuation (about 12 dB) occurring during transmission from the head of the forager to that of the receiver. Vibrations conducted through the substrate between the forager and food receiver are comparatively small with velocity amplitudes of 0.3 mm/s. Possible ways of perception and the advantages of vibration transmission by direct contact within the recruitment context are discussed.
Assuntos
Comunicação Animal , Abelhas/fisiologia , Comportamento Alimentar/fisiologia , Detecção de Sinal Psicológico/fisiologia , Tórax/fisiologia , Vibração , Animais , Comportamento Animal , Tórax/efeitos dos fármacosRESUMO
Foragers of many species of stingless bees guide their nestmates to food sources by means of scent trails deposited on solid substrates between the food and the nest. The corresponding trail pheromones are generally believed to be produced in the mandibular glands, although definitive experimental proof has never been provided. We tested the trail following behavior of recruits of Trigona recursa in field experiments with artificial scent trails branching off from natural scent trails of this stingless bee. First-time recruits (newcomers) did not follow these trails when they were laid with pure solvent or mandibular gland extract. However, they did follow trails made with labial gland extract. Chemical analyses of labial gland secretions revealed that hexyl decanoate was the dominant component (72.4 +/- 1.9% of all volatiles). Newcomers were significantly attracted to artificial trails made with synthetic hexyl decanoate, demonstrating its key function in eliciting scent-following behavior. According to our experiments with T. recursa, the trail pheromone is produced in the labial glands and not in the mandibular glands. Hexyl decanoate is the first component of a trail pheromone identified and proved to be behaviorally active in stingless bees.
Assuntos
Comunicação Animal , Abelhas/fisiologia , Decanoatos/metabolismo , Feromônios/química , Feromônios/fisiologia , Animais , Decanoatos/química , Feromônios/isolamento & purificação , Glândulas Odoríferas/metabolismo , Extratos de Tecidos/químicaRESUMO
Footprint secretions deposited at the nest entrance or on food sources are used for chemical communication by honey bees, bumble bees, and stingless bees. The question of the glandular origin of the substances involved, however, has not been unequivocally answered yet. We investigated the morphology and structure of tarsal glands within the fifth tarsomeres of the legs of workers of Melipona seminigra in order to clarify their possible role in the secretion of footprints. The tarsal gland is a sac-like fold forming a reservoir. Its glandular tissue is composed of a unicellular layer of specialized epidermal cells, which cover the thin cuticular intima forming the reservoir. We found that the tarsal glands lack any openings to the outside and therefore conclude that they are not involved in the secretion of footprint substances. The secretion produced accumulates within the gland's reservoir and reaches as far as into the arolium. Thus it is likely that it serves to fill and unfold the arolium during walking to increase adhesion on smooth surfaces, as is known for honey bees and weaver ants.
Assuntos
Abelhas/anatomia & histologia , Glândulas Endócrinas/anatomia & histologia , Animais , Comportamento Animal , Extremidades/anatomia & histologia , FemininoRESUMO
By depositing scent marks on flowers, bees reduce both the search time and the time spent with the handling of nonrewarding flowers. They thereby improve the efficiency of foraging. Whereas in honey bees the source of these scent marks is unknown, it is assumed to be the tarsal glands in bumble bees. According to histological studies, however, the tarsal glands lack any openings to the outside. Foragers of the stingless bee Melipona seminigra have previously been shown to deposit an attractant pheromone at sugar solution feeders, which is secreted at the tips of their tarsi. Here we show that the claw retractor tendons have specialized glandular epithelia within the femur and tibia of all legs that produce this pheromone. The secretion accumulates within the hollow tendon, which also serves as the duct to the outside, and is released from an opening at the base of the unguitractor plate. In choice experiments, M. seminigra was attracted by feeders baited with pentane extracts of the claw retractor tendons in the same way as it was attracted by feeders previously scent marked by foragers. Our results resolve the seeming contradiction between the importance of foot print secretions and the lack of openings of the tarsal glands.