The estimation of fructosamine and HbAlc in pregnant women with diabetes mellitus.

Fructosamine, HbAlc, glucose, albumins and total proteins were estimated in 40 healthy pregnant women and 80 pregnant women with insulin dependent diabetes mellitus. Fructosamine was estimated by the NBT method with "Fructosamine test" commercially available kit on Technicom automatic analyser RA-1000. Glucose was determined on Beckman glucose analyser. HbAlc was assayed by the Bio-Rad test, while albumin and total proteins by Beckman tests. For all estimated parameters no significant differences were found between healthy pregnant women and pregnant women with insulin dependent diabetes mellitus.

[Correlation of glycosylated hemoglobin and fructosamine in pregnant women with diabetes mellitus]. / Korelacija glikolizovanog hemoglobina i fruktozamina kod trudnica s dijabetesom melitusom.

Glycolisated hemoglobin (HBA1c), fructosamine, glucose, albumin and total proteins were estimated 40 healthy pregnant women and 90 pregnant women with diabetes mellitus. Fructosamine was estimated by NBT method with "Fructosamine test" commercially available kit on Technicom automatic analyser RA-1000. Glucose was determined on Beckmman glucose analyser. HBA1c was assayed by Bio-Rad test, while albumin and total proteins by Beckmman tests. We found best correlation between fructosamine and HBA1c at pregnant women who were on dietary therapy worst at pregnancy women on insulin therapy.

[Single-fraction radiotherapy of painful bone metastases]. / Radioterapija bolnih kostnih metastaza jednom frakcijom.

We analyzed first results obtained in 92 patients treated with single-dose fraction radiotherapy for painful bone metastases. Tumour doses was 10 Gy in a single fraction. Response was obtained in 59/92 (64%) patients. Thirty nine patients had complete response and 20 had partial response. Pain recurred in 18/39 patients who initially responded. Toxicity of this radiotherapeutic treatment is acceptable, and can easily be managed with standard therapy.

[The opioid peptide, beta-endorphin, in spontaneous vaginal delivery and cesarean section]. / Opioidni peptid--beta-endorfin u toku spontanog vaginalnog porodjaja i carskog reza.

The opioid peptide, beta-endorphin, originates from proopiomelanocortin (POMC) under the influence of corticotropin releasing hormone (CHR). It increases the threshold of pain and has a certain influence on the formation of hypophyseal hormones, especially in stress. It is found that beta-endorphin stimulates the secretion of prolactin, a growth hormone, and vasopressin; it inhibates formation of follicle-stimulating and luteinizating hormones, oxytocin and dopamine, and gonadotropin, a releasing hormone. The process of acetylization decreases its activity. The results of experimental trials revealed that acetylisation in the foetal period was absent. The aim of the study was to define beta-endorphin concentration during normal vaginal labor and Cesarean section. Samples of peripheral blood of patients with spontaneous vaginal labor (n = 15) and of those in whom labor was operatively terminated (Cesarean section) (n = 10), were analysed. Values of this opiate were determined in the umbilical cord of newborn infants, in the amniotic fluid and placental compartment. The obtained results were statistically analysed. In intrapartum beta-endorphins were significantly increased reaching the highest level during expulsion (326 pg/ml); in the placental compartment these values were higher (in retroplacental blood 514 pg/ml) reaching the highest value of 917 pg/ml, p less than 0.01 in the placenta. In Cesarean section beta-endorphin values in the peripheral blood showed no significant differences during spontaneous vaginal labor. However, increased values of this natural opiate were observed six hours after surgery. Beta-endorphin concentrations in the placental compartment and the placenta during normal vaginal labor were significantly higher in comparison with labor by Cesarean section (p less than 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)

[The effect of complete thymic extract on motility and progressive motility of spermatozoa in asthenozoospermia]. / Dejstvo celokupnog ekstrakta timusa na motilitet i progresivni motilitet spermatozoida kod astenozoospermije.

The thymic gland is involved in immunologic and endocrinologic heomostasis of the body. Neonatal thymectomy of experimental animals leads to gonadal dysgenesis. Both eostrogen and androgen receptors have been identified in thymic epitelial cells, and sex steroids can be modulated by secretion of thymosin. High levels of thymosin alpha-1 in seminal plasma are correlated with sperm count, viability and motility in fertile males as compared to infertile ones. In this study we examined the effects of complete thymic extract on motility and progressive motility of spermatozoa obtained from asthenozoospermic men. In the first part of experiment, we studied the influence of complete thymic extract, as a bioactive medium component, on movement of spermatozoa samples with initial motility lower than 30%, in vitro. The study was carried out on two groups of sperm samples, 40 samples each, obtained by masturbation after a 72-hour-abstinence. After completed liquefaction at room temperature (30-40 min.), we determined the usual parameters of sperm quality. Total motility (M%) and progressive motility (PM%) of spermatozoa were analyzed in 10 microscopic view fields. Each group of sperm samples was prepared by swim-up and swim-down method. Then, we estimated the total motility and progressive motility of "washed" spermatozoa in every sample. In addition, every sample was divided in two equal parts. One part was immediately left in thermostat (control). To the second part we added 0.2 mg of complete thymic extract before incubation (experimental group). Total and progressive motilities were analyzed after 1, 4, 12 and 24 hours of incubation. The t-test was used for statistical analysis of results. The second part of the study was carried out on a group of 10 asthenozoospermic men (volunteers). We examined the influence of complete thymic extract, as intra-muscular injection, in a doze of 150 mg daily, during seven days, on motility and progressive motility of spermatozoa. Sperm samples were obtained by masturbation after four days of abstinence. We compared the parameters before and after the therapy. They were statistically processed by a method of difference. The results showed that the complete thymic extract increased the total and progressive motilities of spermatozoa separated by swim-down method (p < 0.001), in vitro (Table 1-4). The parenteral application of complete thymic extract, statistically significantly increased motility and progressive motility of spermatozoa in asthenozoospermic men (p < 0.05) (Table 5). These findings could be useful in assisted reproductive technology.

[Clinical and immunologic aspects of chlamydial infections in infertile women]. / Klinicki i imunoloski aspekti hlamidijalnih infekcija kod infertilnih zena.

UNLABELLED: Chlamydia Trachomatis (CT) infection of the genital tract ranges from clinically severe to the silent subclinical forms of pelvic inflammatory diseases which can result in fallopian tubes obstruction and subsequent infertility. Our investigations: The serum antichlamydial antibodies of 30 infertile patients and the cultures of Chlamydia Trachomatis from specimens of endometrial biopsies were determined as well as HSG and laparoscopic examinations. METHODOLOGY: The evaluation of serum antichlamydial antibodies were measured by ELISA test. The endometrial specimens for culture of Chlamydia Trachomatis were collected transcervically from the uterine cavity. The statistical analyses were calculated on the basis of Student t test. RESULTS: Immunological determinations showed the presence of antichlamydial antibodies in 43.6%. The endometrial cultures yielded growth of CT (36.6%) and histological signs of chlamydial endometritis were also discovered. This study pointed to the positive correlation between the results of endometrial biopsies (cultures), immunological (serological) investigations and clinical examinations showing that the chlamydial infection reached the Fallopian tubes from the endometrium which could result in infertility.