High time-resolved studies of stick-slip show similar dilatancy to fast and slow earthquakes.

Fast and slow earthquakes are two modes of energy release by the slip in tectonic fault rupture. Although fast and slow slips were observed in the laboratory stick-slip experiments, due to the sampling rate limitation, the details of the fault thickness variation were poorly understood. Especially, why a single fault would show different modes of slip remains elusive. Herein, we report on ring shear experiments with an ultrahigh sampling rate (10 MHz) that illuminate the different physical processes between fast and slow slip events. We show that the duration of slips ranged from dozens to hundreds of milliseconds. Fast slip events are characterized by continuous large-amplitude AE (acoustic emission) and somewhat intricate variation of the sample thickness: A short compaction pulse during the rapid release of stress is followed by dilation and vibrations of the sample thickness. As the slip ends, the thickness of the sample first recovers by slow compaction and then dilates again before nucleation of the following slip event. In contrast, during slow slip events, the shear stress reduction is accompanied by intermittent bursts of low-amplitude AE and sample dilation. We observed the detailed thickness variation during slips and found that dilation occurs during both fast and slow slips, which is consistent with natural observations of coseismic dilatation. This study may be used to reveal the mechanism of fault slips during fast and slow earthquakes, which explain the potential effect of fast and slow slips on stress redistribution and structural rearrangement in faults.

Damping-Driven Time Reversal for Waves.

Damping is usually associated with irreversibility. Here, we present a counterintuitive concept to achieve time reversal of waves propagating in a lossless medium using a transitory dissipation pulse. Applying a sudden and strong damping in a limited time generates a time-reversed wave. In the limit of a high damping shock, this amounts to "freezing" the initial wave by maintaining the wave amplitude while canceling its time derivative. The initial wave then splits in two counterpropagating waves with half of its amplitude and time evolutions in opposite directions. We implement this damping-based time reversal using phonon waves propagating in a lattice of interacting magnets placed on an air cushion. We show with computer simulations that this concept also applies to broadband time reversal in complex disordered systems.

Changes in water use and wastewater generation influenced by the COVID-19 pandemic: A case study of China.

This paper examines and projects the water use and wastewater generation during and after the SARS-CoV-2 (COVID-19) in China, and discussed the water use/wastewater generation pattern changes among different sectors. Existing studies on the impact of pandemic spread-prevention measures on water consumption and wastewater treatment during the pandemic are reviewed. The water use and wastewater discharge in China through the COVID-19 period are then projected and analyzed using Multivariate Linear Regression. The projection is carried out for years 2019-2023 and covers an (estimated) full process of pre-pandemic, pandemic outbreak, and recovery phase and provides essential information for determining the complete phase impact of the COVID-19. Two scenarios, i.e. the recovery scenario and the business as usual scenario, are set to investigate the water use and wastewater generation characteristics after the pandemic. The results imply that in both scenarios, the water use in China shows a V-shaped trend from 2019 to 2023 and reached a low point in 2020 of 5,813✕108 m3. The wastewater discharge shows an increasing trend throughout the COVID period in both scenarios. The results are also compared with the water consumption and wastewater generation during the SARS-CoV-1 period. The implication for policymakers is the possible increase of water use and wastewater discharge in the post COVID period and the necessity to ensure the water supply and control of water pollution and wastewater discharge.

Industrial water network vulnerability analysis using dynamic inoperability input-output model.

Industrial parks provide opportunities for Process Integration among different enterprises. Inter-Plant Water Network Integration is an effective strategy for water conservation. However, increased interplant linkages can make the entire system vulnerable to cascading failures in case of loss of water flow in some plants. The potential indirect impact of water shortages on such integrated systems may not be evident without the use of appropriate models. This work defines inoperability as the fractional loss of water flow relative to normal operations. A comparison between the applicability of demand-driven versus supply-driven Inoperability Input-output Model (IIM) is conducted. Then, a Vulnerability Assessment Framework which integrates vulnerability indicators into the Dynamic Input-Output Model (DIIM) is developed to analyse failure propagation in water networks in an industrial park. The DIIM is then applied to simulate the cascading effects of disturbances. From a time perspective, the vulnerabilities of the industrial parks With Integrated Optimal Water Network (WWN) and Without Integrated Optimal Water Network (WOWN) are assessed considering robustness, adaptability, and recoverability as the indicators. The results indicate that supply-driven IIM is more suitable for cascading failure analysis of water networks. The average inoperability at 16% from supply-driven IIM is higher than that from demand-driven IIM. In the freshwater disturbance scenario, the dependence of the plant on freshwater is proportional to the rate of inoperability change, the time to reach a new equilibrium. In this study, the robustness of WWN is about fivefold that of WOWN, but the recovery rate is only one-sixth of the latter. This work can help identify system vulnerabilities and provide a scientific insight for the development of park-wide water management strategies.

Virtual Water Scarcity Risk to the Global Trade System.

Local water scarcity risk (LWSR, meaning potential economic output losses in water-using sectors due to physical water scarcity) can be transmitted to downstream economies through the globalized supply chains. To understand the vulnerability of the global economy to water scarcity, we examine the impacts of local water scarcity risk on the global trade system from 1995 to 2009. We observe increasingly intensified geographical separation between physical water scarcity and production losses due to water scarcity. We identify top nation-sectors in virtual water scarcity risk (VWSR) exports (indicating local water scarcity risk in each nation transmitted to foreign nations through its exports), including agriculture and utilities in major economies such as China, India, Spain, France, and Turkey. These nation-sectors are critical to the resilience of the global economy to water scarcity. We also identify top nation-sectors in virtual water scarcity risk imports (indicating each nation's vulnerability to foreign water scarcity risk through the global trade system), highlighting their vulnerability to distant water scarcity. Our findings reveal the need for nations to collaboratively manage and conserve water resources, and lay the foundation for firms in high VWSR-importing sectors to develop strategies to mitigate such risk.

Transcriptomic responses of Nile tilapia (Oreochromis niloticus) liver to environmental concentration of di(2-ethylhexyl)phthalate.

Di-(2-ethylhexyl) phthalate (DEHP) is currently the most frequently detected phthalic acid esters (PAEs) compounds and can induce diverse toxicities on aquatic organisms. To understand the molecular responses of fish to DEHP, we performed transcriptomic profiles in liver of tilapia (Oreochromis niloticus) which were exposed to environmental concentration of DEHP. A total of 30.10 Mb and 30.16 Mb clean reads were retrieved from the control and DEHP treated libraries, respectively. De-novo assembly of all the clean reads obtained 58,585 unigenes. After comparing the two libraries, 2814 and 1790 genes were identified as significantly increased and depressed, respectively. Gene ontology (GO) classification system and Kyoto Encyclopedia of Genes and Genomes (KEGG) database analysis demonstrated that DEHP significantly disturbed the expression level of genes associated with immunity, endocrine and reproductive system, lipid metabolism and so on. Quantitative real-time PCR was performed to validate the results of RNA-sequencing (RNA-seq) analysis. The resulting data provide new insights for exploring the molecular basis of tilapia response to DEHP exposure.

Transcriptional response of four C1q domain containing protein (C1qDC) genes from Venerupis philippinarum exposed to the water soluble fraction of No.0 diesel oil.

As pattern recognitionreceptors, the C1q-domain-containing (C1qDC) proteins play an important role in the pathogen recognition and complement pathway activation. In the present study, four novel C1q domain containing proteins (designated as VpC1qDC1, VpC1qDC2, VpC1qDC3 and VpC1qDC4) were cloned and characterized from clam Venerupis philippinarum. The four VpC1qDCs all possessed the conserved features critical for the fundamental structure and function of the C1q family. The four VpC1qDCs genes showed differential response profiles after exposure to the water soluble fraction of No.0 diesel oil (WSFD). More notably, VpC1qDC1 and VpC1qDC3 were more sensitive to low concentration of WSFD, as their mRNA level changed by higher magnitudes. In addition, VpC1qDC2 and VpC1qDC4 displayed notable increases with larger amplitude to high concentration of WSFD. All these results suggested that the transcriptional response of VpC1qDCs genes were probably a protective mechanism of the cell to oils pollution. The diverse expression patterns of VpC1qDCs demonstrated that VpC1qDC1 and VpC1qDC3 were sensitive responders to environmental stress in V. philippinarum.

Thermosensitive molecularly imprinted hydrogel cross-linked with N-malely chitosan for the recognition and separation of BSA.

A novel temperature-sensitive molecularly imprinted hydrogel composed of N-isopropylacrylamide and acrylamide has been prepared by using free-radical polymerization and was cross-linked by modified water-soluble N-maley chitosan in aqueous solution. BSA (pI 4.9, MW 66.0 kDa) was used as the template protein. The produced hydrogels were characterized by environmental SEM to reveal the microcosmic morphology. A microporous structure was only found in the imprinted hydrogel, while no obvious microporous structure was found in nonimprinted hydrogels. The lower critical solution temperature of the hydrogels was 34°C, and the optimal binding conditions were tested, namely, the adsorption equilibrium time of 6 h and initial BSA concentration of 1.0 mg/mL. The adsorption capacity Qmax was determined by Langmuir isotherm plots and was 5.72 mg/g for imprinted hydrogel and 1.18 mg/g for nonimprinted hydrogels. A separation factor (ß) of 4 was obtained when bovine hemoglobin (pI 6.9, MW 64.0 kDa) was selected as the particular reference protein. Molecular weights and pIs were chosen to investigate the selectivity of the hydrogels. It was shown that the shape memory and the size effect were the major factors for the recognition. This imprinted hydrogel was used to specifically adsorb the BSA from the protein mixture.

Acoustic monitoring of compaction in cohesive granular materials.

We study the transition from cohesive to noncohesive states of cemented granular materials (synthetic rocks) under oedometric loading, combining simultaneous measurements of ultrasound velocity and acoustic emission (AE: microseosmicity). Our samples are agglomerates made of glass beads bonded with a few percent of cement, either ductile or brittle. These cemented granular samples exhibit an inelastic compaction beyond certain axial stresses likely due to the formation of compaction bands, which is accompanied by a significant decrease of compressional wave velocity. Upon subsequent cyclic unloading-reloading with constant consolidation stress, we found the mechanical and acoustic responses like those in noncohesive granular materials, which can be interpreted within the effective medium theory based on the Digby's bonding model. Moreover, this model allows P-wave velocity measured at vanishing pressure to be interpreted as an indicator of the debonding on the scale of grain contact. During the inelastic compaction, stick-slip-like stress drops were observed in brittle cement-bonded granular samples accompanied by the instantaneous decrease of the P-wave velocity and AEs which display an Omori-like law for foreshocks, i.e., precursors. By contrast, mechanical responses of ductile cement-bonded granular samples are smooth (without visible stick-slip-like stress drops) and mostly aseismic. By applying a cyclic loading-unloading with increasing consolidation stress, we observed a Kaiser-like memory effect in the brittle cement-bonded sample in the weakly damaged state which tends to disappear when the bonds are mostly broken in the noncohesive granular state after large-amplitude loading. In this paper, we show that the macroscopic ductile and brittle behavior of cemented granular media is controlled by the local processes on the scale of the bonds between grains.

Polydopamine-based molecular imprinting on silica-modified magnetic nanoparticles for recognition and separation of bovine hemoglobin.

Surface molecular imprinting, especially on the surface of silica-modified magnetic nanoparticles, has been proposed as a promising strategy for protein recognition and separation. Inspired by the self-polymerization of dopamine, we synthesized a polydopamine-based molecular imprinted film coating on silica-Fe(3)O(4) nanoparticles for recognition and separation of bovine hemoglobin (BHb). Magnetic molecularly imprinted nanoparticles (about 860 nm) possess a core-shell structure. Magnetic molecularly imprinted nanoparticles (MMIP) show a relatively high adsorption capacity (4.65 ± 0.38 mg g(-1)) and excellent selectivity towards BHb with a separation factor of 2.19. MMIP with high saturation magnetization (10.33 emu g(-1)) makes it easy to separate the target protein from solution by an external magnetic field. After three continuous adsorption and elution processes, the adsorption capacity of MMIP remained at 4.30 mg g(-1). Our results suggest that MMIPs are suitable for the removal of high abundance of protein and the enrichment of low abundance of protein in proteomics.

Differential response of two ferritin subunit genes (VpFer1 and VpFer2) from Venerupis philippinarum following pathogen and heavy metals challenge.

As a principal extracellular iron storage molecule, ferritin plays an important role in the iron-withholding strategy of innate immunity and detoxification system. In this study, we cloned and characterized another ferritin from Venerupis philippinarum (designated as VpFer2), in addition to one previously reported (VpFer1). VpFer2 possessed all the conserved features critical for the fundamental structure and function of ferritin H subunit. VpFer1 and VpFer2 mRNA were both found to be most abundantly expressed in hepatopancreas. Vibrio challenge could significantly up-regulate the mRNA expression of VpFers, and VpFer2 showed more sensitive to Vibrio anguillarum infection. For heavy metals exposure, the expression level of VpFer1 was significantly induced by Cd at 48 h, but kept relatively constant after exposure to Cu. With regards to VpFer2, the expression level dropped significantly at 24 h, then began to increase to the peak value at 48 h under Cd exposure, while Cu exposure constantly depressed the expression level of VpFer2 throughout the time course. Similarly, VpFer2 seemed to be more sensitive to heavy metals exposure than VpFer1 as its mRNA level changed by higher magnitudes. All these results suggested that VpFers may be important proteins involved in host immune defense and heavy metals detoxification. The diverse expression patterns of VpFers demonstrated that VpFer2 was an early and sensitive responder to environmental stress in V. philippinarum.

Establishment of prediction models to predict survival among patients with cervical cancer based on socioeconomic factors: a retrospective cohort study based on the SEER Database.

OBJECTIVE: To construct and validate predictive models based on socioeconomic factors for predicting overall survival (OS) in cervical cancer and compare them with the American Joint Council on Cancer (AJCC) staging system. DESIGN: Retrospective cohort study. SETTING AND PARTICIPANTS: We extracted data from 5954 patients who were diagnosed with cervical cancer between 2007 and 2011 from the Surveillance, Epidemiology, and End Results Database. This database holds data related to cancer incidence from 18 population-based cancer registries in the USA. OUTCOME MEASURES: 1-year and 5-year OS. RESULTS: Of the total 5954 patients, 5820 patients had 1-year mortality and 5460 patients had 5-year mortality. Lower local education level [Hazard ratios (HR): 1.15, 95% confidence interval (CI): 1.04 to 1.27, p= 0.005] and being widowed (HR 1.28, 95% CI 1.06 to 1.55, p=0.009) were associated with a worse OS for patients with cervical cancer. Having insurance (HR 0.75, 95% CI 0.62 to 0.90, p=0.002), earning a local median annual income of ≥US$56 270 (HR 0.83, 95% CI 0.75 to 0.92, p<0.001) and being married (HR 0.79, 95% CI 0.69 to 0.89, p<0.001) were related to better OS in patients with cervical cancer. The predictive models based on socioeconomic factors and the AJCC staging system had a favourable performance for predicting OS in cervical cancer compared with the AJCC staging system alone. CONCLUSION: Our proposed predictive models exhibit superior predictive performance, which may highlight the potential clinical application of incorporating socioeconomic factors in predicting OS in cervical cancer.

Oyster arsenic, cadmium, copper, mercury, lead and zinc levels in the northern South China Sea: long-term spatiotemporal distributions, combined effects, and risk assessment to human health.

Estuarine and coastal ecosystems are often considered vulnerable due to the complex biogeochemical processes and the human disturbances through a variety of pollution. Among environmental contaminants, heavy metals in estuarine and coastal ecosystems have been of increasing concern in environmental conservation. Long-term exposure to heavy metal contamination, mainly through food and water, could be harmful to human health. It is therefore critical to understand the quantitative comparisons and combined effects of different heavy metals in common seafood species, such as oysters. This work studied the long-term spatiotemporal trends and health risk assessment of oyster arsenic (As), cadmium (Cd), copper (Cu), mercury (Hg), lead (Pb), and zinc (Zn) levels in the coastal waters of northern South China Sea. Cultured oysters (Crassostrea rivularis) from 23 estuaries and harbors in the coastal areas of northern South China Sea in 1989-2015 were analyzed for the spatiotemporal trends of the six heavy metal levels. Metal pollution index (MPI), target hazard quotient (THQ), and hazard index (HI) were used for quantifying the exposure of the six heavy metals to human health through oyster consumption. Principal component analysis (PCA) was used for assessing the relative importance of the six metals in oyster heavy metal distribution patterns in the northern South China Sea. Overall, the As, Cd, Cu, Hg, Pb, and Zn levels in oysters from the northern South China Sea generally declined from 1989 to 2015, stayed relatively high (MPI = 2.42-3.68) during 1989-2000, gradually decreased since 2000, and slightly increased after 2010. Oyster heavy metal levels were highest in the Pearl River Estuary (MPI = 1.20-5.52), followed by west Guangdong and east Guangdong, Guangxi, and Hainan coastal waters. This pattern is probably because economics and industry around the Pearl River Estuary have been growing faster than the other areas of this work in the recent two decades, and it should be taken as a hotspot for the monitoring of seafood safety in southern China. Principal component analysis indicated that Cu, Zn, and Cd were the most important metals in the long-term distributions of oyster heavy metal levels in the northern South China Sea. Health risk assessment suggested that the risk of the six heavy metals exposure through oyster consumption were relatively high during 1989-2005 (THQ = 1.01-5.82), significantly decreased since 2005 (THQ < 1), and slightly increased after 2010.

Multi-leveled insights into the response of the eelgrass Zostera marina L to Cu than Cd exposure.

Seagrass beds are recognized as critical and among the most vulnerable habitats on the planet; seagrass colonize the coastal waters where heavy metal pollution is a serious problem. In this study, the toxic effects of copper and cadmium in the eelgrass Zostera marina L. were observed at the individual, subcellular, physiologically biochemical, and molecular levels. Both Cu and Cd stress significantly inhibited the growth and the maximal quantum yield of photosystem II (Fv/Fm); and high temperature increased the degree of heavy metal damage, while low temperatures inhibited damage. The half-effect concentration (EC50) of eelgrass was 28.9 µM for Cu and 2246.8 µM for Cd, indicating Cu was much more toxic to eelgrass than Cd. The effect of Cu and Cd on photosynthesis was synergistic. After 14 days of enrichment, the concentration of Cu in leaves and roots of Z. marina was 48 and 37 times higher than that in leaf sheath, and 14 and 11 times higher than that in rhizome; and the order of Cd concentration in the organs was root > leaf > rhizome > sheath. Heavy metal uptake mainly occurred in the organelles, and Cd enrichment also occurred to a certain extent in the cytoplasm. Transcriptome results showed that a number of photosynthesis-related KEGG enrichment pathways and GO terms were significantly down-regulated under Cd stress, suggesting that the photosynthetic system of eelgrass was severely damaged at the transcriptome level, which was consistent with the significant inhibition of Fv/Fm and leaf yellowing. Under Cu stress, the genes related to glutathione metabolic pathway were significantly up-regulated, together with the increased autioxidant enzyme activity of GSH-PX. In addition, the results of recovery experiment indicated that the damage caused by short-term Cd and Cu stress under EC50 was reversible. These results provide heavy metal toxic effects at multiple levels and information relating to the heavy metal resistance strategies evolved by Z. marina to absorb and isolate heavy metals, and highlight the phytoremediation potential of this species especially for Cd.

[The responsive characteristics of phytochrome genes to photoperiod, abiotic stresses and identification of their key natural variation sites in foxtail millet (Setaria italica L.)].

The responsive patterns of phytochrome gene family members to photoperiod and abiotic stresses were comparatively analyzed and the favorable natural variation sites of these genes were identified. This would help understand the mechanism of phytochrome gene family in photoperiod-regulated growth and development and abiotic stress response. In addition, it may facilitate the molecular marker assisted selection of key traits in foxtail millet. In this study, we used RT-PCR to clone three phytochrome genes SiPHYA, SiPHYB and SiPHYC from ultra-late maturity millet landrace variety 'Maosu'. After primary bioinformatics analysis, we studied the photoperiod control mode and the characteristics of these genes in responding to five abiotic stresses including polyethylene glycol (PEG)-simulated drought, natural drought, abscisic acid (ABA), high temperature and NaCl by fluorescence quantitative PCR. Finally, we detected the mutation sites of the three genes among 160 foxtail millet materials and performed haplotype analysis to determine the genes' functional effect. We found that the cloned cDNA sequences of gene SiPHYA, SiPHYB and SiPHYC were 3 981, 3 953 and 3 764 bp respectively, which contained complete coding regions. Gene SiPHYB and SiPHYC showed closer evolutionary relationship. Photoperiod regulated all of the three genes, but showed more profound effects on diurnal expression pattern of SiPHYB, SiPHYC than that of SiPHYA. Under short-day, when near heading, the expression levels of SiPHYA and SiPHYB were significantly lower than that under long-day, indicating their roles in suppressing heading of foxtail millet under long-day. SiPHYB and SiPHYC were responsive to PEG-simulated drought, natural drought, ABA and high temperature stresses together. SiPHYA and SiPHYB responded differently to salt stress, whereas SiPHYC did not respond to salt stress. Re-sequencing of 160 foxtail millet materials revealed that SiPHYB was highly conservative. Two missense mutations of SiPHYA, such as single nucleotide polymorphism (SNP) 7 034 522C→T and SNP7 036 657G→C, led to delaying heading and increasing plant height. One missense mutation of SiPHYC, such as SNP5 414 823G→T, led to shortening heading under short-day and delaying heading under long-day, as well as increasing plant height and panicle length regardless of photo-thermal conditions. Photoperiod showed different regulatory effects on SiPHYA, SiPHYB and SiPHYC. SiPHYB and SiPHYC jointly responded to various abiotic stresses except for the salt stress. Compared with the reference genotype, mutation genotypes of SiPHYA and SiPHYC delayed heading and increased plant height and panicle length.

Nonlinear dynamics, granular media and dynamic earthquake triggering.

The 1992 magnitude 7.3 Landers earthquake triggered an exceptional number of additional earthquakes within California and as far north as Yellowstone and Montana. Since this observation, other large earthquakes have been shown to induce dynamic triggering at remote distances--for example, after the 1999 magnitude 7.1 Hector Mine and the 2002 magnitude 7.9 Denali earthquakes--and in the near-field as aftershocks. The physical origin of dynamic triggering, however, remains one of the least understood aspects of earthquake nucleation. The dynamic strain amplitudes from a large earthquake are exceedingly small once the waves have propagated more than several fault radii. For example, a strain wave amplitude of 10(-6) and wavelength 1 m corresponds to a displacement amplitude of about 10(-7) m. Here we show that the dynamic, elastic-nonlinear behaviour of fault gouge perturbed by a seismic wave may trigger earthquakes, even with such small strains. We base our hypothesis on recent laboratory dynamic experiments conducted in granular media, a fault gouge surrogate. From these we infer that, if the fault is weak, seismic waves cause the fault core modulus to decrease abruptly and weaken further. If the fault is already near failure, this process could therefore induce fault slip.

Antioxidant responses and bioaccumulation in green-lipped mussels (Perna viridis) under acute tributyltin chloride exposure.

Green-lipped mussels (Perna viridis) were exposed to waterborne tributyltin chloride (TBTCl) at different concentrations (0.2, 0.4 and 0.8 µg L(-1)) for up to 72 h. Accumulated TBTCl in Perna viridis correlated linearly with the exposure concentrations of 0.2 µg L(-1)(R(2) = 0.772), 0.4 µg L(-1)(R(2) = 0.952), and 0.8 µg L(-1)(R(2) = 0.909). The results of superoxide dismutase (SOD), glutathione peroxidase (GPx) and malondialdehyde (MDA) all decreased in gill tissues after 24 h of exposure, but the hepatic SOD and the hepatic GPx showed either little or no effect on exposure of TBTCl solutions. Analysis using the Spearman rank correlation coefficient showed the hepatic GPx activity appeared to have a significant negative correlativity (R(s) = -0.42) with the exposed TBTCl concentrations, and the hepatic MDA was significantly negatively correlated (R(s) = -0.33) with the tissue TBTCl concentrations. Conversely, a significant positive correlation (R(s) = 0.60) was shown between the gill MDA contents and exposure time. This study illustrates oxyradical scavenger GPx best correlated with stress level of pollutants among the various antioxidant parameters.

Distinct expression of three estrogen receptors in response to bisphenol A and nonylphenol in male Nile tilapias (Oreochromis niloticus).

Environmental estrogens, such as bisphenol A (BisA) and nonylphenol (NP), have been shown to affect the estrogen receptor (ER) expression and induce male reproductive abnormalities. To elucidate molecular mechanisms of action of xenoestrogenic chemicals on the expression of estrogen receptors in the testes of Nile tilapia (Oreochromis niloticus), three full-length cDNAs respectively encoding ntERalpha, ntERbeta1 and ntERbeta2 were cloned from testes. The amino acid sequences of ntERalpha, ntERbeta1 and ntERbeta2 showed a high degree of similarity to the relevant fish species. Tissue-specific expression study showed that three receptors were highly expressed in pituitary, liver, testis, kidney and intestine tissues. The ntERalpha, ntERbeta1 and ntERbeta2 mRNA expressions were significantly higher at the sexual early recrudescing stage than at other recrudesced stages. After being exposed to xenoestrogens from weeks 2 to 4, the ntERalpha mRNA levels were increased significantly in testes after NP treatment at all sampling times or after 4 weeks of exposure to BPA. The ntERbeta1 mRNA levels remained unchanged, while a significant decrease of the ntERbeta2 mRNA level was observed in testes after exposure to NP and BPA. The present study demonstrates that the regulation of all three ntER subtypes in testes may act via different molecular mechanisms of exposure to NP and BPA.

Oyster copper levels in the northern South China Sea from 1989 to 2015: spatiotemporal trend detection and human health implications.

Coastal heavy metal pollution has become an important topic for seafood safety and marine environmental protection. Unlike toxic heavy metals such as cadmium or chromium, copper is essential for oysters' growth but can inhibit their immune response to exotic stress when going above normal levels. Oysters with high copper levels can easily accumulate and transfer abnormal amounts of copper to upper trophic levels, and generate health risks for humans. This study investigated the spatiotemporal variability and health risk of copper levels in cultured oysters (Crassostrea rivularis) sampled from 23 harbors, bays, or estuaries along the northern South China Sea during 1989-2015. Overall, oyster copper concentrations in the study area ranged from 0.9 to 1897.0 µg/g wet weight with a mean of 210.0 (± 143.6) µg/g and a median of 89.3 µg/g. Although oyster copper levels in the southern China provinces of Guangdong, Guangxi, and Hainan showed an overall decrease during 1989-2015, they stayed relatively low since 1996 and increased slightly after 2010. Oyster copper levels in Guangdong were significantly higher than in Hainan and Guangxi. In Guangdong, oyster copper levels were highest in the Pearl River Estuary, followed by west Guangdong and east Guangdong. The health risk of copper exposure through oyster consumption increased in 2011-2015 compared with in 2006-2010. It is recommended that the human daily intake of cultured oysters in the study area should be reduced by half to minimize copper exposure. This study suggested that copper is one of the most important heavy metal contaminants in coastal and estuarine ecosystems of the northern South China Sea.

The associations between neutrophil-to-lymphocyte ratio and the Chinese Visceral Adiposity Index, and carotid atherosclerosis and atherosclerotic cardiovascular disease risk.

BACKGROUND: Inflammation and obesity are the main risk factors for the development of carotid atherosclerosis and atherosclerotic cardiovascular disease (ASCVD). The neutrophil-to-lymphocyte ratio (NLR) is a recently developed indicator of inflammation that can be easily calculated from blood cell counts. The Chinese Visceral Adiposity Index (CVAI) was used to assess visceral obesity in the Chinese population. AIMS: To explore the associations between both NLR and CVAI, and carotid atherosclerosis and ASCVD risk in elderly Chinese. METHODS: A total of 4437 participants aged ≥55 years and with no history of cardiovascular disease, were enrolled in this retrospective study. Anthropometric measurements, laboratory results, and carotid ultrasonography results were extracted from a database. We used established formulas to calculate NLR and CVAI, and the Pooled Cohort Equations to generate the 10-year ASCVD risk score. Participants were divided into two groups according to their 10-year ASCVD scores: <7.5% and ≥7.5%. RESULTS: NLR and CVAI were significantly higher in patients with carotid atherosclerosis. Regression analysis showed that NLR (OR 1.23, 95% CI 1.05-1.43, p = 0.01) and CVAI (OR 1.39, 95% CI 1.21-1.61, p = 0.001) were independent risk factors for carotid atherosclerosis. A combination of NLR and CVAI improved the goodness-of-fit (p < 0.001) and discriminability of the model (p = 0.0013). NLR, CVAI and carotid plaques showed positive associations with the 10-year ASCVD risk score (all p < 0.001). CONCLUSIONS: NLR and CVAI are positively associated with the prevalence of carotid atherosclerosis and high risk of ASCVD in elderly adults and could be useful in the identification of a high risk of atherosclerosis.