Increased suppression of methane production by humic substances in response to warming in anoxic environments.

Humic substances (HS) are redox-active and can function as organic terminal electron acceptors in anaerobic microbial respiration, which plays a relevant role on suppressing the emissions of methane (CH4) in anoxic systems. However, it is unclear whether or not there is an inherent link between suppression of CH4 emissions by HS and warming temperature. In this study, we assess the effects of HS additions on CH4 production in paddy and wetland soils and their responses to increasing temperature by incubation experiments. We show that the intensity of HS to suppress CH4 production under anoxic condition is positively associated with the temperature, which may be due to the fact that the activities of enzymes involved in methanogenesis have lower temperature sensitivity than those involved in microbial HS reduction, and that the methanogenesis process is less susceptible to increasing temperature compared to the microbial HS reduction process. The hypothetical increase in the effectiveness of pH alteration and HS toxicity caused by warming may be also responsible for the increased inhibition of CH4 production by HS addition in response to increasing temperature. Our findings highlight the increasingly important role of HS in suppressing CH4 production in anoxic ecosystems in a future warmer world.

Increased Electron-Accepting and Decreased Electron-Donating Capacities of Soil Humic Substances in Response to Increasing Temperature.

The electron transfer capacities (ETCs) of soil humic substances (HSs) are linked to the type and abundance of redox-active functional moieties in their structure. Natural temperature can affect the chemical structure of natural organic matter by regulating their oxidative transformation and degradation in soil. However, it is unclear if there is a direct correlation between ETC of soil HS and mean annual temperature. In this study, we assess the response of the electron-accepting and -donating capacities (EAC and EDC) of soil HSs to temperature by analyzing HSs extracted from soil set along glacial-interglacial cycles through loess-palaeosol sequences and along natural temperature gradients through latitude and altitude transects. We show that the EAC and EDC of soil HSs increase and decrease, respectively, with increasing temperature. Increased temperature facilitates the prevalence of oxidative degradation and transformation of HS in soils, thus potentially promoting the preferentially oxidative degradation of phenol moieties of HS or the oxidative transformation of electron-donating phenol moieties to electron-accepting quinone moieties in the HS structure. Consequently, the EAC and EDC of HSs in soil increase and decrease, respectively. The results of this study could help to understand biogeochemical processes, wherein the redox functionality of soil organic matter is involved in the context of increasing temperature.

Decoupling Distribution of n-Alkanes in Aeolian Sand and Vegetation of the Northern Ulan Buh Desert, China: Insight into Organic Matter Preservation in Arid Regions.

Fallen leaves and their decomposition directly deposit leaf wax n-alkanes into sediments, which can be used to identify local flora. These n-alkanes are important for studying past vegetation and climate, but their distribution in sediments must be known. Aeolian sand n-alkanes are particularly important for understanding paleoclimates in arid regions, despite the challenges of extraction due to their extremely low abundance. To investigate the preservation of plant leaf wax n-alkanes in deserts, we analyzed n-alkanes in aeolian sands from the Northern Ulan Buh Desert (UBD), China, and compared them to the surrounding vegetation. We calculated the total n-alkane concentration (ΣALK), average chain length (ACL21-35), and carbon preference index (CPI21-35). In the Northern UBD, aeolian sand n-alkanes have lower ΣALK, indicating microbial degradation. The eastern aeolian sand has lower CPI21-35 and ACL21-35 than the adjacent vegetation, whereas the western sand values are consistent with the plants, likely due to the transport of plant-derived materials by wind and water from the nearby mountains. Our study shows that sedimentary n-alkane signatures are not only determined by local vegetation but also influenced by environmental factors like temperature and precipitation. Additionally, local deposition processes play a significant role in determining the properties of these n-alkanes.

Addressing the Relationship between Leaf Nitrogen and Carbon Isotope Discrimination from the Three Levels of Community, Population and Individual.

The carbon, nitrogen and water cycles of terrestrial ecosystems are important biogeochemical cycles. Addressing the relationship of leaf nitrogen (N) and carbon isotope discrimination (Δ) will enhance the understanding of the links between these three cycles in plant leaves because Δ can reflect time-integrated leaf-level water-use efficiency (WUE) over the period when the leaf material is produced. Previous studies have paid considerable attention to the relationship. However, these studies have not effectively eliminated the interference of environmental factors, inter-species, and inter-individual differences in this relationship, so new research is necessary. To minimize these interferences, the present work explored the relationship at the three levels of community, population, and plant individual. Three patterns of positive, negative and no relationship were observed across communities, populations, and individuals, which is dependent on environmental conditions, species, and plant individuals. The results strongly suggested that there is no general pattern for the relationship between leaf N and Δ. Furthermore, the results indicated that there is often no coupling between leaf-level long-term WUE and leaf N in the metabolic process of carbon, N and water in leaves. The main reason for the lack of this relationship is that most plants do not invest large amounts of nitrogen into photosynthesis. In addition, the present study also observed that, for most plant species, leaf N was not related to photosynthetic rate, and that variations in photosynthetic rates are mainly driven by stomatal conductance.

Examination of the negative correlation between leaf δ15N and the N:P ratio across a northeast-southwest transect in China.

Nitrogen (N) and phosphorus (P) are two crucial limiting mineral elements for terrestrial plants. Although the leaf N:P ratio is extensively used to indicate plant nutrient limitations, the critical N:P ratios cannot be universally applied. Some investigations have suggested that leaf nitrogen isotopes (δ15N) can provide another proxy for nutrient limitations along with the N:P ratio, but the negative relationships between N:P and δ15N were mainly limited to fertilization experiments. It will obviously benefit the study of the nature of nutrient limitations if the relationship could be explained more generally. We analyzed leaf δ15N, N, and P contents across a northeast-southwest transect in China. Leaf δ15N was weakly negatively correlated with leaf N:P ratios for all plants, while there was no correlation between them for various plant groups, including different growth forms, genera, and species across the entire N:P range. This suggests that the use of leaf δ15N in indicating the shift of nutrient limitations across the whole N:P range still requires more validated field investigations. Notably, negative relationships between δ15N and N:P hold for plants with N:P ratios between 10 and 20 but not for plants with N:P ratios lower than 10 or higher than 20. That is, changes in leaf δ15N along with the N:P ratio of plants that are co-limited by N and P can exhibit variations in plant nutrient limitations, whereas plants that are strictly limited by N and P cannot. Moreover, these relationships are not altered by vegetation type, soil type, MAP, or MAT, indicating that the use of leaf δ15N in reflecting shifts in nutrient limitations, depending on the plant nutrient limitation range, is general. We examined the relationships between leaf δ15N and the N:P ratio across an extensive transect, providing references for the widespread use of leaf δ15N in reflecting shifts in nutrient limitation.

Clarifying the influence of temperature on variances in plant metallic nutrients through minimizing the effect of precipitation.

Understanding the responses of plant nutrients to climate warming is important in the research of global change. However, the responses of plant metallic nutrients to climate warming have been rarely addressed. Furthermore, in previous field investigations, the influence of temperature on plant metallic nutrients has been not effectively separated from that of precipitation; hence, there exists some uncertainties in the relationships between plant metallic nutrients and temperature. To minimize the effect of precipitation, this study collected plant samples over broad geographical scale along the 400 mm isohyet in China with a temperature span of 14.8 °C. The temperature effects on variations in leaf potassium (K), calcium (Ca), magnesium (Mg), iron (Fe), manganese (Mn) and zinc (Zn) were assessed. For all species pooled together, leaf Ca and Mg kept relatively stable, whereas leaf K, Fe, Mn and Zn decreased with increasing temperature. The responses of leaf Ca, Mg and Mn to changing temperature were almost similar at functional group, genus and species levels and independent of vegetation and soil type. It suggested that the relationships between leaf Ca, Mg and Mn and temperature should be general results. However, the patterns of leaf K, Fe and Zn vs. temperature varied across functional groups, genera and species and were affected by vegetation and soil type, which indicated that the observed patterns were local phenomena. Our results suggested that global warming might have no effect on leaf Ca and Mg, but could decrease leaf K, Fe, Mn and Zn.

Accelerated Microbial Reduction of Azo Dye by Using Biochar from Iron-Rich-Biomass Pyrolysis.

Biochar is widely used in the environmental-protection field. This study presents the first investigation of the mechanism of biochar prepared using iron (Fe)-rich biomass and its impact on the reductive removals of Orange G dye by Shewanella oneidensis MR-1. The results show that biochars significantly accelerated electron transfer from cells to Orange G and thus stimulated reductive removal rate to 72-97%. Both the conductive domains and the charging and discharging of surface functional groups in biochars played crucial roles in the microbial reduction of Orange G to aniline. A high Fe content of the precursor significantly enhanced the conductor performance of the produced biochar and thus enabled the biochar to have a higher reductive removal rate of Orange G (97%) compared to the biochar prepared using low-Fe precursor (75%), but did not promote the charging and discharging capacity of the produced biochar. This study can prompt the search for natural biomass with high Fe content to confer the produced biochar with wide-ranging applications in stimulating the microbial reduction of redox-active pollutants.

Minimizing the effect of precipitation in clarifying the responses of leaf N and P stoichiometry to temperature.

How terrestrial ecosystem responds to global warming has received wide attention. Plant stoichiometry has the potential to reflect ecosystem responses to climate change, thus, investigating the variations in plant stoichiometry with temperature is important and necessary for revealing the responses of terrestrial ecosystem to global warming. Although many studies had explored the relationships between plant N, P stoichiometry and temperature, previous field investigations did not eliminate the interference of precipitation effect with these observed relationships. To minimize the effect of precipitation on leaf N, P stoichiometry, this investigation was conducted across a temperature gradient over broad geographical scale along the 400 mm isohyet, which extends about 6000 km in China. This study showed that leaf N did not vary, whereas leaf P decreased and leaf N:P ratio increased with increasing mean annual temperature (MAT). The responses of leaf N and P stoichiometry to MAT observed in this study might be general patterns; because they were almost ubiquitous across functional groups, genera and species examined, and moreover, they were independent of vegetation and soil type. It could be inferred from this study that global warming in future will have no effect on leaf N, but reduce leaf P and increase leaf N:P ratio. Stable leaf N and varied leaf P with changing MAT suggested that leaf N and P decoupled with changing temperature.

Discrepant responses of methane emissions to additions with different organic compound classes of rice straw in paddy soil.

Crop straw incorporation has become a prevailing agricultural practice that guarantees the food production and security. There is a significant body of work on the effects of straw incorporation on the methane (CH4) emissions in paddy fields. However, it is unclear whether there are diverse links between CH4 emission dynamics and incorporations of different organic compound classes of straw to paddy fields. In this study, soil incubations were conducted to assess the respective effect of incorporations of hydrolysable amino acid (HAA), dilute-acid extractable carbohydrate (DAC), lipid and acid-insoluble organic matter (AIOM) fractions of rice straw on the CH4 emission in paddy soil. It is revealed that incorporations of HAA and DAC fractions exert the greatest intensities to stimulate the CH4 emissions, which mainly takes place in the early period of incubation; on contrary, the incorporation of lipid fraction exerts the lowest intensity and mainly takes place in the late period. The pattern of CH4 emission after incorporation of AIOM fraction occurs peaks both in the early and late periods of incubation. Our findings highlight that the time of occurrence and intensity of effects of rice straw incorporation on CH4 emissions vary significantly depending on the different organic compound classes of rice straw, which may be key to proposing a promising management strategy for mitigating CH4 emissions in paddy fields in the context of straw incorporation.

Foliar δ13C Showed No Altitudinal Trend in an Arid Region and Atmospheric Pressure Exerted a Negative Effect on Plant δ13C.

Previous studies have suggested foliar δ13C generally increases with altitude. However, some observations reported no changes or even decreased trends in foliar δ13C. We noted that all the studies in which δ13C increased with elevation were conducted in the human regions, whereas those investigations in which δ13C did not vary or decreased were conducted in areas with water stress. Thus, we proposed that the pattern of increasing δ13C with elevation is not a general one, and that δ13C may remain unchanged or decrease in plants grown in arid environments. To test the hypothesis, we sampled plants along altitude gradients on the shady and sunny slopes of Mount Tianshan characterized by arid and semiarid climates. The measurements of foliar δ13C showed no altitudinal trends for the plants grown on either of the slopes. Therefore, this study supported our hypothesis. In addition, the present study addressed the effect of atmospheric pressure on plant δ13C by accounting for the effects of temperature and precipitation on δ13C. This study found that the residual foliar δ13C increased with increasing altitude, suggesting that atmospheric pressure played a negative role in foliar δ13C.

Effects of environmental and biotic factors on carbon isotopic fractionation during decomposition of soil organic matter.

Decomposition of soil organic matter (SOM) plays an important role in the global carbon cycle because the CO2 emitted from soil respiration is an important source of atmospheric CO2. Carbon isotopic fractionation occurs during SOM decomposition, which leads to (12)C to enrich in the released CO2 while (13)C to enrich in the residual SOM. Understanding the isotope fractionation has been demonstrated to be helpful for studying the global carbon cycle. Soil and litter samples were collected from soil profiles at 27 different sites located along a vertical transect from 1200 to 4500 m above sea level (a.s.l.) in the south-eastern side of the Tibetan Plateau. Their carbon isotope ratios, C and N concentrations were measured. In addition, fiber and lignin in litter samples were also analyzed. Carbon isotope fractionation factor (α) during SOM decomposition was estimated indirectly as the slope of the relationship between carbon isotope ratios of SOM and soil C concentrations. This study shows that litter quality and soil water play a significant role in isotope fractionation during SOM decomposition, and the carbon isotope fractionation factor, α, increases with litter quality and soil water content. However, we found that temperature had no significant impact on the α variance.