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1.
Ecotoxicol Environ Saf ; 276: 116301, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38599159

RESUMO

To study the heavy metal accumulation and its impact on insect exterior and chromosome morphology, and reveal the molecular mechanism of insects adapting to long-term heavy metal compound pollution habitats, this study, in the Diaojiang river basin, which has been polluted by heavy metals(HMs) for nearly a thousand years, two Eucriotettix oculatus populations was collected from mining and non-mining areas. It was found that the contents of 7 heavy metals (As, Cd, Pb, Zn, Cu, Sn, Sb) in E. oculatus of the mining area were higher than that in the non-mining 1-11 times. The analysis of morphology shows that the external morphology, the hind wing type and the chromosomal morphology of E. oculatus are significant differences between the two populations. Based on the heavy metal accumulation,morphological change, and stable population density, it is inferred that the mining area population has been affected by heavy metals and has adapted to the environment of heavy metals pollution. Then, by analyzing the transcriptome of the two populations, it was found that the digestion, immunity, excretion, endocrine, nerve, circulation, reproductive and other systems and lysosomes, endoplasmic reticulum and other cell structure-related gene expression were suppressed. This shows that the functions of the above-mentioned related systems of E. oculatus are inhibited by heavy metal stress. However, it has also been found that through the significant up-regulation of genes related to the above system, such as ATP2B, pepsin A, ubiquitin, AQP1, ACOX, ATPeV0A, SEC61A, CANX, ALDH7A1, DLD, aceE, Hsp40, and catalase, etc., and the down-regulation of MAPK signalling pathway genes, can enhanced nutrient absorption, improve energy metabolism, repair damaged cells and degrade abnormal proteins, maintain the stability of cells and systems, and resist heavy metal damage so that E. oculatus can adapt to the environment of heavy metal pollution for a long time.


Assuntos
Gafanhotos , Metais Pesados , Poluentes Químicos da Água , Animais , Metais Pesados/toxicidade , Poluentes Químicos da Água/toxicidade , Gafanhotos/efeitos dos fármacos , Gafanhotos/anatomia & histologia , Monitoramento Ambiental/métodos , Mineração , China , Adaptação Fisiológica/efeitos dos fármacos , Transcriptoma/efeitos dos fármacos , Rios/química
2.
Chemistry ; 29(48): e202301121, 2023 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-37300353

RESUMO

Lithium-sulfur (Li-S) batteries are a promising energy storage technology due to their tempting high theoretical capacity and energy density. Nevertheless, the wastage of active materials that originates from the shuttling effect of polysulfides still hinders advancement of Li-S batteries. The effective design of cathode materials is extremely pivotal to solve this thorny problem. Herein, surface engineering in covalent organic polymers (COPs) has been performed to investigate the influence of pore wall polarity on the performance of COP-based cathodes used for Li-S batteries. With the assistance of experimental investigation and theoretical calculations, performance improvement by increasing pore surface polarity and a synergy effect of the polarized functionalities, along with nano-confinement effect of the COPs, are disclosed, to which the improved performance of Li-S batteries including outstanding Coulombic efficiency (99.0 %) and extremely low capacity decay (0.08 % over 425 cycles at 1.0 C) is attributed. This work not only enlightens the designable synthesis and applications of covalent polymers as polar sulfur hosts with high utilization of active materials, but also provides a feasible guide for the design of effective cathode materials for future advanced Li-S batteries.

3.
Org Biomol Chem ; 21(17): 3691-3696, 2023 May 03.
Artigo em Inglês | MEDLINE | ID: mdl-37070775

RESUMO

We report a Brønsted acid-catalyzed C6 functionalization of 2,3-disubstituted indoles with 2,2-diarylacetonitriles for efficient construction of cyano-substituted all-carbon quaternary centers with excellent yields. The synthetic utility was demonstrated by the conversion of the cyano-group which enables the divergent preparation of aldehydes, primary amines and amides. Control experiments suggested that this process involves C-H oxidation of 2,2-diarylacetonitriles to in situ generate δ,δ-disubstituted p-quinone methide intermediates. This protocol provides an efficient method for C6 functionalization of 2,3-disubstituted indoles to construct all-carbon quaternary centers.

4.
J Am Chem Soc ; 139(19): 6736-6743, 2017 05 17.
Artigo em Inglês | MEDLINE | ID: mdl-28445639

RESUMO

Covalent organic frameworks (COFs) are an emerging class of crystalline porous organic materials which are fabricated via reticular chemistry. Their topologic structures can be precisely predicted on the basis of the structures of building blocks. However, constructing COFs with complicated structures has remained a great challenge, due to the limited strategies that can access to the structural complexity of COFs. In this work, we have developed a new approach to produce COFs bearing three different kinds of pores. The design is fulfilled by the combination of vertex-truncation with multiple-linking-site strategy. On the basis of this design, a "V"-shaped building block carrying two aldehyde groups on the end of each branch has been synthesized. Condensation of it with 1,4-diaminobenzene or benzidine leads to the formation of two triple-pore COFs, TP-COF-DAB and TP-COF-BZ, respectively. The topological structures of the triple-pore COFs have been confirmed by PXRD studies, synchrotron small-angle X-ray scattering (SAXS) experiments, theoretical simulations, and pore size distribution analyses. Furthermore, for the first time, an in situ COF-to-COF transformation has also been achieved by heating TP-COF-BZ with 1,4-diaminobenzene under solvothermal condition, which leads to the formation of TP-COF-DAB via in situ replacing the benzidine linkers in TP-COF-BZ with 1,4-diaminobenzene linkers.

5.
J Org Chem ; 82(10): 5441-5448, 2017 05 19.
Artigo em Inglês | MEDLINE | ID: mdl-28471176

RESUMO

The di-tert-butyl peroxide (DTBP) induced coupling of arenesulfonylindoles with ethers such as 1,4-dioxane, tetrahydropyran, tetrahydrofuran, and 1,2-dimethoxyethane was studied. The distinguishing feature of this strategy was characterized by capturing in situ generated vinylogous imine intermediates for the C(sp3)-H bond alkylation of ethers. This general procedure presents the major advantages of its wide substrate scope and good functional group compatibility.

6.
Org Biomol Chem ; 15(6): 1325-1328, 2017 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-28102406

RESUMO

An efficient access to optically active sulfahydantoins, 4-aryl-1,2,5-thiadiazolidin-3-one 1,1-dioxides, was developed through palladium-catalyzed asymmetric hydrogenation of the corresponding cyclic N-sulfonylketimines with up to 98% ee.

7.
Chemistry ; 22(49): 17784-17789, 2016 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-27778380

RESUMO

Integrating different kinds of pores into one covalent organic framework (COF) endows it with hierarchical porosity and thus generates a member of a new class of COFs, namely, heteropore COFs. Whereas the construction of COFs with homoporosity has already been well developed, the fabrication of heteropore COFs still faces great challenges. Although two strategies have recently been developed to successfully construct heteropore COFs from noncyclic building blocks, they suffer from the generation of COF isomers, which decreases the predictability and controllability of construction of this type of reticular materials. In this work, this drawback was overcome by a multiple-linking-site strategy that offers precision construction of heteropore COFs containing two kinds of hexagonal pores with different shapes and sizes. This strategy was developed by designing a building block in which double linking sites are introduced at each branch of a C3 -symmetric skeleton, the most widely used scaffold to construct COFs with homogeneous porosity. This design provides a general way to precisely construct heteropore COFs without formation of isomers. Furthermore, the as-prepared heteropore COFs have hollow-spherical morphology, which has rarely been observed for COFs, and an uncommon staggered AB stacking was observed for the layers of the 2D heteropore COFs.

8.
Org Biomol Chem ; 14(29): 6937-41, 2016 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-27345438

RESUMO

A chiral phosphoric acid-catalyzed asymmetric transfer hydrogenation of 3-trifluoromethylthioquinolines has been successfully developed, providing direct and facile access to chiral 2,3-disubstituted 1,2,3,4-tetrahydroquinoline derivatives containing a stereogenic trifluoromethylthio group with up to 99% enantioselectivity.

9.
J Insect Sci ; 162016.
Artigo em Inglês | MEDLINE | ID: mdl-26810560

RESUMO

The sweet potato leaf folder, Brachmia macroscopa, is an important pest in China. The complete mitogenome, which consists of 13 protein-coding genes (PCGs), 22 transfer RNA genes, two ribosomal RNA genes, and an A + T-rich region, was sequenced and found to be 15,394 bp in length (GeneBank no. KT354968). The gene order and orientation of the B. macroscopa mitogenome were similar to those of other sequenced lepidopteran species. All of the PCGs started with ATN as the canonical start codon except for cox1, which started with CGA. In regard to stop codons, most PCGs stopped at TAA except for cox2, which stopped at TA, and nad4, which stopped at a single T. Thirteen PCGs of the available species (33 taxa) were used to demonstrate phylogenetic relationships. The ditrysian cluster was supported as a monophyletic clade at high levels by using maximum likelihood and Bayesian methods. The apoditrysian group, covering the Gelechioidea, formed a monophyletic clade with a bootstrap value of 88% and a posterior probability of 1.00. The superfamily Gelechioidea was supported as a monophyletic lineage by a posterior probability of 1.00.


Assuntos
Genoma de Inseto/genética , Mitocôndrias/genética , Mariposas/genética , Animais , Filogenia , Reação em Cadeia da Polimerase , RNA Ribossômico/genética
10.
Molecules ; 19(12): 19665-77, 2014 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-25438078

RESUMO

A mild and efficient method catalyzed by α-chymotrypsin was developed for the synthesis of bis(indolyl)methanes through a cascade process between indole and aromatic aldehydes. In the ethanol aqueous solution, a green medium, a wide range of aromatic aldehydes could react with indole to afford the desired products with moderate to good yields (from 68% to 95%) using a little α-chymotrypsin as catalyst.


Assuntos
Biocatálise , Química Orgânica/métodos , Quimotripsina/metabolismo , Indóis/síntese química , Metano/síntese química , Animais , Bovinos , Etanol/química , Indóis/química , Metano/química , Sus scrofa , Temperatura
11.
Mol Genet Genomics ; 288(11): 627-38, 2013 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-23996145

RESUMO

Studies on the cockroach have contributed to our understanding of several important developmental processes, especially those that can be easily studied in the embryo. However, our knowledge on late events such as gonad differentiation in the cockroach is still limited. The major aim of the present study was to identify sex-specific genes between adult female and male Periplaneta americana. Two cDNA libraries were constructed using the suppression subtractive hybridization method; a total of 433 and 599 unique sequences were obtained from the forward library and the reverse library, respectively, by cluster assembly, and sequence alignment of 1,032 expressed sequence tags. The analysis of the differentially expressed gene functions allowed these genes to be categorized into three groups: biological process, molecular function, and cellular component. The differentially expressed genes were suggested to be related to the development of the gonads of P. americana. Twelve differentially expressed genes were randomly selected and verified using relative quantitative real-time polymerase chain reaction (qRT-PCR). Meanwhile, by adopting a range of filtering criteria, we predicted two potential microRNA sequences for P. americana, pam-miR100-3p and pam-miR7. To confirm the expression of potential microRNAs (miRNAs) in American cockroach, a qRT-PCR approach was also employed. The data presented here offer the insights into the molecular foundation of sex differences in American cockroach, and the first report for the miRNAs in this species. In addition, the results can be used as a reference for unraveling candidate genes associated with the sex and reproduction of cockroaches.


Assuntos
Regulação da Expressão Gênica , MicroRNAs/genética , Hibridização de Ácido Nucleico/métodos , Periplaneta/genética , Animais , Sequência de Bases , DNA Complementar/genética , Etiquetas de Sequências Expressas , Feminino , Perfilação da Expressão Gênica , Biblioteca Gênica , Masculino , Dados de Sequência Molecular , Especificidade de Órgãos , Ovário/fisiologia , Periplaneta/fisiologia , Filogenia , Alinhamento de Sequência , Análise de Sequência de DNA , Fatores Sexuais , Testículo/fisiologia
12.
Molecules ; 18(11): 13910-9, 2013 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-24284489

RESUMO

An environmentally-friendly, enzyme-promoted procedure for the Henry reaction was first studied using water-in-[Bmim][PF6] microemulsions as reaction medium. The Amano acylase from Aspergillus oryzae showed better catalytic activity for the addition reactions of nitromethane with a series of aromatic aldehydes, and a highest yield of 90% was obtained.


Assuntos
Amidoidrolases/metabolismo , Aspergillus oryzae/enzimologia , Aldeídos/química , Aldeídos/metabolismo
13.
Curr Genet ; 58(2): 65-77, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-22311390

RESUMO

The mitochondrial genomes are one of the most information-rich markers in phylogenetics. The relationships within superorder Dictyoptera have been debated in the literature. However, the closely related termites (Isoptera) are retained as unranked taxon within the order Blattaria (cockroaches). In this work, we sequenced the complete mitogenomes of two cockroaches, reconstructed the molecular phylogeny and attempted to infer the phylogenetic position of termites in Blattaria more reliably. The complete mtDNA nucleotide sequences of the peridomestic American cockroach (Periplaneta americana L.) and the domestic German cockroach (Blattella germanica L.) are 15,025 and 15,584 bp in size, respectively. The genome shares the gene order and orientation with previously known Blattaria mitogenomes. Most tRNAs could be folded into the typical cloverleaf secondary structure, but the tRNA-Ser (AGN) of P. americana appears to be missing the dihydrouridine arm. Using nucleotide and amino acid sequences as phylogenetic markers, we proposed that termites should be treated as a superfamily (Termitoidea) of cockroaches. We suggested that Polyphagoidea was the sister group of Termitoidea in Blattaria and supported that the suborder Caelifera is more closely related to the Phasmatodea than to the suborder Ensifera of Orthoptera.


Assuntos
Baratas/genética , Genoma Mitocondrial , Isópteros/genética , Periplaneta/genética , Filogenia , Animais , Sequência de Bases , Ordem dos Genes , Dados de Sequência Molecular
14.
Metabolites ; 12(9)2022 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-36144209

RESUMO

Holometabolism is a form of insect development which includes four life stages: egg, larva, pupa, and imago (or adult). The developmental change of whole body in metabolite levels of holometabolous insects are usually ignored and lack study. Diapause is an alternative life-history strategy that can occur during the egg, larval, pupal, and adult stages in holometabolous insects. Kallima inachus (Lepidoptera: Nymphalidae) is a holometabolous and adult diapausing butterfly. This study was intended to analyze metabolic changes in K. inachus during ontogeny and diapause through a non-targeted UPLC-MS/MS (ultra-performance liquid chromatograph coupled with tandem mass spectrometry) based metabolomics analysis. A variety of glycerophospholipids (11), amino acid and its derivatives (16), and fatty acyls (nine) are crucial to the stage development of K. inachus. 2-Keto-6-acetamidocaproate, N-phenylacetylglycine, Cinnabarinic acid, 2-(Formylamino) benzoic acid, L-histidine, L-glutamate, and L-glutamine play a potentially important role in transition of successive stages (larva to pupa and pupa to adult). We observed adjustments associated with active metabolism, including an accumulation of glycerophospholipids and carbohydrates and a degradation of lipids, as well as amino acid and its derivatives shifts, suggesting significantly changed in energy utilization and management when entering into adult diapause. Alpha-linolenic acid metabolism and ferroptosis were first found to be associated with diapause in adults through pathway analyses. Our study lays the foundation for a systematic study of the developmental mechanism of holometabolous insects and metabolic basis of adult diapause in butterflies.

15.
Mol Biol Rep ; 38(6): 3757-64, 2011 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-21107722

RESUMO

We determined the nucleotide sequence of the mitochondrial genome (mtgenome) of Spilonota lechriaspis Meyrick (Lepidoptera: Tortricidae). The entire closed circular molecule is 15,368 bp and contains 37 genes with the typical gene complement and order for lepidopteran mtgenomes. All tRNAs except tRNASer(AGN) can be folded into the typical cloverleaf secondary structures. The protein-coding genes (PCGs) have typical mitochondrial start codons, with the exception of COI, which uses the unusual CGA one as is found in all other Lepidoptera sequenced to date. In addition, six of 13 PCGs harbor the incomplete termination codons, a single T. The A+T-rich region contains some conserved structures that are similar to those found in other lepidopteran mtgenomes, including a structure combining the motif 'ATAGA', a 19-bp poly(T) stretch and three microsatellite (AT)n elements which are part of larger 122+ bp macrorepeats. This is the first report of macrorepeats in a lepidopteran mtgenome.


Assuntos
Genoma Mitocondrial/genética , Lepidópteros/genética , Animais , Composição de Bases/genética , Sequência de Bases , Códon/genética , DNA Intergênico/genética , Complexo IV da Cadeia de Transporte de Elétrons/genética , Dados de Sequência Molecular , Conformação de Ácido Nucleico , Fases de Leitura Aberta/genética , RNA Ribossômico/genética , RNA de Transferência/química , RNA de Transferência/genética , Especificidade da Espécie , Sequências de Repetição em Tandem/genética
16.
Org Lett ; 23(7): 2393-2398, 2021 Apr 02.
Artigo em Inglês | MEDLINE | ID: mdl-33734717

RESUMO

The direct regio- and enantioselective C6 functionalization of 2,3-disubstituted indoles with azadienes has been developed using chiral phosphoric acid as catalyst, providing a convenient approach to synthesize the optically active heterotriarylmethanes with excellent yields, broad substrate scope, and up to 98% ee. Mechanistic studies revealed that N-alkylation of 2,3-disubstituted indoles with azadienes would be reversible, and enantioselective C6 functionalization could be enabled.

17.
Mol Biol Rep ; 37(8): 3877-88, 2010 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-20213506

RESUMO

The nearly complete mitochondrial genome of the butterfly Papilio xuthus (Lepidoptera: Papilionidae) was sequenced for its nucleotide sequence of 13,964 bp. The genome has a typical gene order identical to other lepidopteran species. All tRNAs showed same stable canonical clover-leaf structure as those of other insects, except for tRNA(Ser) (AGN), in which the dihydrouracil arm (DHU arm) could not form stable stem-loop structure. Anomalous initiation codons have been observed for the cox1 gene, where the ATTACG hexa-nucleotide was believed to be involved in the initiation signaling. Twelve mitochondrial protein-coding gene sequence data were used to infer the phylogenetic relationships among the insect orders. Even though the number of insect orders represented by complete mitochondrial genomes is still limited, several well-established relationships are evident in the phylogenetic analysis of the complete sequences. Monophyly of the Homometabola was not supported in this paper. Phylogenetic analyses of the available species of Bombycoidea, Pyraloidea, Papilionoidea and Tortricidea bolstered the current morphology-based hypothesis that Bombycoidea, Pyraloidea and Papilionoidea are monophyletic (Obtectomera). Bombycoidea (Bombyx mandarina and Antheraea pernyi) and Papilionoidea (P. xuthus and Coreana raphaelis) formed a sister group.


Assuntos
Borboletas/genética , Genoma de Inseto/genética , Genoma Mitocondrial/genética , Filogenia , Animais , Composição de Bases/genética , Sequência de Bases , Teorema de Bayes , Ordem dos Genes/genética , Dados de Sequência Molecular , Conformação de Ácido Nucleico , Nucleotídeos/genética , RNA Ribossômico/química , RNA Ribossômico/genética , RNA de Transferência/química , RNA de Transferência/genética , Análise de Sequência de DNA
18.
Mol Biol Rep ; 37(7): 3509-16, 2010 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-20012368

RESUMO

We present the complete mitochondrial DNA sequence of Eupolyphaga sinensis. This closed circular molecule is 15553 bp long and consists of 37 genes that encode for 13 inner membrane proteins, 2 ribosomal RNAs and 22 transfer RNAs. The genome shares the gene order and orientation with previously known Blattaria mitochondrial genomes. All tRNAs could be folded into the typical cloverleaf secondary structure, but the tRNASer (AGN) appears to be missing the DHU arm. The A + T-rich region is 857 bp long and longer than other cockroaches. Based on the concatenated amino acid sequences of all protein coding genes of E. sinensis in conjunction with those 23 other arthropod sequences, we reconstruct the phylogenetic tree. Phylogenetic analyses shows that Blataria (including Isoptera) and the Mantodea are sister groups. Furthermore the relationship of the three basal clades of winged insects are different from the three previous hypotheses ((Ephemeroptera + Odonata) +Neoptera, Ephemeroptera + (Odonata + Neoptera), Odonata + (Ephemeroptera +Neoptera)). The Ephemeroptera (Parafronurus youi) clusters with the Plecoptera (Pteronarcys princes).


Assuntos
Baratas/genética , Genoma Mitocondrial/genética , Filogenia , Sequência de Aminoácidos , Animais , Composição de Bases/genética , Códon/genética , Genes de Insetos/genética , Fases de Leitura Aberta/genética , RNA Ribossômico/genética , RNA de Transferência/genética
19.
Front Physiol ; 11: 889, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33013431

RESUMO

Studies of chemosensory genes are key to a better understanding of intra- and interspecific communications between insects and their environment and provide opportunities for developing environmentally friendly pesticides to target pest species. The bamboo locust Ceracris kiangsu Tsai (Orthoptera: Acrididae) is one of the most important bamboo leaf-eating insects in southern China. However, the genes underlying olfactory sensation are lacking in the bamboo locust. In this study, the transcriptomes of male and female C. kiangsu antennae were sequenced and analyzed. A total of 125 chemosensory genes, including 91 odorant receptors (ORs), 13 ionotropic receptors (IRs), 13 odorant-binding proteins (OBPs), six chemosensory proteins (CSPs), and two sensory neuron membrane proteins, were identified based on sequence alignment and phylogenetic analyses. The expression patterns of all candidate genes on the antennae of males and females, maxillary palps, tarsi, wings, and thoraxes-abdomens were confirmed by real-time quantitative PCR. The analyses demonstrated that most genes are highly expressed in the antennae, and 35 ORs, 7 IRs, 10 OBPs, and 1 CSP exhibit significantly male-biased expression patterns, indicating their potential functions in mating behavior and the recognition of female sex pheromones. In addition to the antennal-predominant genes, some were abundant in the maxillary palps and some in the non-olfactory tissues, suggesting their different functions in the olfactory system of C. kiangsu. Our research offers an extensive resource for investigating the chemoreception mechanism of C. kiangsu. Further studies of olfactory function will provide comprehensive methods and original strategies for integrated pest management.

20.
Chem Commun (Camb) ; 56(98): 15418-15421, 2020 Dec 21.
Artigo em Inglês | MEDLINE | ID: mdl-33231586

RESUMO

Transformation between 2D covalent organic frameworks (COFs) via exchange of molecular building blocks with different symmetries has been realized, which gives rise to the conversion between 2D COFs with distinct pore hierarchy. This type of monomer replacement has expanded the scope of the building-unit-exchange-based COF-to-COF transformation strategy.

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