[HPLC Fingerprint Study and Quantitative Determination of Index Components of Pilea aquarum].

OBJECTIVE: To establish an HPLC fingerprint and a quantitative determination method for determination of index components of Pilea aquarum. METHODS: The HPLC fingerprint of Pilea aquarum were determined on an Agilent Zorbax SB-C18 column (250 mm x 4.6 mm, 5 µm), eluted with mobile phase containing of acetonitrile-0.2% acetic acid in a gradient mode. The temperature of column was 30 °C. The flow rate was 0.8 mL/min and the detection wavelength was set at 330 nm. The chromatograms of 24 batches of Pilea aquarum were compared by the software of Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (version 2004 A). The contents of luteoloside and cosmosiin were also determined simultaneously. RESULTS: The HPLC fingerprint of Pilea aquarum had been established. There were ten common peaks,two chromatographic peaks of which were identified by reference substances. The samples of Pilea aquarum from different habitats can be distinguished from their fingerprints, the contents of luteoloside and cosmosiin varied greatly. CONCLUSION: This method has desirable precision, stability, repeatability, and can be applied for identification and quality control of Pilea aquarum.

[Variation of both DNA genetic structure and reproduction traits of plant autotetraploid].

Due to the effect of chromosome doubling, the DNA genetic structures of autotetraploid vary from its original diploid, and thus autotetraploid phenotype changes correspondingly. Compared with original diploid, the phenotype changes of autotetraploid were as follows. The part of its male and female gametes was of abortion. Its pollen (diplo-haplont) was significantly bigger. The number of egg cells or synergids or antipodal cells in its embryo sacs increases or reduces. While self-crossing procreation of it, pollen tubes and the fertilization processes of polar nucleus and the fertilized cells development were partly abnormal. Its reproductive capacity (or seed setting rate) dropped to some extents, which can be gradually improved with generations. For some plant species, it has a better cross-compatibility in distant hybridizations.