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Biochem Soc Trans ; 34(Pt 6): 1241-5, 2006 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-17073794

RESUMO

Elucidation of metabolic pathways of xenobiotics (pesticides, pharmaceuticals and industrial pollutants) in human, animals and plants and chemical identification of corresponding metabolites are required for comprehensive (eco-) toxicological evaluation of the compounds prior to their usage. The most important metabolic products are oxidized metabolites, and most of these are formed by catalytic activity of P450s (cytochrome P450 mono-oxygenases). In human, 11 P450 isoenzymes exhibiting broad and overlapping substrate specificities are responsible for approx. 90% of drug metabolism. As support for inevitable metabolism studies with intact organisms under relevant conditions, tobacco cell cultures were transformed separately with cDNA sequences of human P450 isoenzymes CYP1A1, CYP1A2 and CYP3A4. The resulting P450-transgenic cell suspensions were used for metabolism studies with pesticides, industrial pollutants, a secondary plant metabolite and human sex hormones. A summary of basic results is provided; these are discussed regarding application of the method for screening of the oxidative metabolism of xenobiotics and the large-scale production of metabolites.


Assuntos
Sistema Enzimático do Citocromo P-450/metabolismo , Xenobióticos/metabolismo , Animais , Biotransformação , Técnicas de Cultura de Células/métodos , Células Cultivadas , Citocromo P-450 CYP1A1/genética , Citocromo P-450 CYP1A2/genética , Citocromo P-450 CYP3A , Sistema Enzimático do Citocromo P-450/genética , Humanos , Mamíferos , Plantas/enzimologia , Plantas Geneticamente Modificadas/enzimologia , Proteínas Recombinantes/metabolismo , Nicotiana/citologia , Nicotiana/enzimologia
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