RESUMO
Surface functionalization of hydroxyapatite (HA) and beta-tricalcium phosphate (TCP) bioceramics with chemical ligands containing a pyrrogallol moiety was developed to improve the adhesion of bone cell precursors to the biomaterials. Fast and biocompatible copper-free click reaction with azido-modified human fetal osteoblasts resulted in improved cell binding to both HA and TCP bioceramics, opening the way for using this methodology in the preparation of cell-engineered bone implants.
Assuntos
Materiais Biocompatíveis/química , Fosfatos de Cálcio/química , Adesão Celular , Cerâmica/química , Durapatita/química , Feto/metabolismo , Osteoblastos/metabolismo , Materiais Biocompatíveis/metabolismo , Fosfatos de Cálcio/metabolismo , Proliferação de Células , Células Cultivadas , Cerâmica/metabolismo , Química Click , Durapatita/metabolismo , Feto/citologia , Humanos , Teste de Materiais , Estrutura Molecular , Osteoblastos/citologia , Propriedades de SuperfícieRESUMO
The chemical functionalization of cell-surface proteins of human primary fetal bone cells with hydrophilic bioorthogonal intermediates was investigated. Toward this goal, chemical pathways were developed for click reaction-mediated coupling of alkyne derivatives with cellular azido-expressing proteins. The incorporation via a tetraethylene glycol linker of a dipeptide and a reporter biotin allowed the proof of concept for the introduction of cell-specific peptide ligands and allowed us to follow the reaction in living cells. Tuning the conditions of the click reaction resulted in chemical functionalization of living human fetal osteoblasts with excellent cell survival.
Assuntos
Alcinos/química , Química Click , Proteínas de Membrana/química , Osteoblastos/citologia , Membrana Celular/química , Sobrevivência Celular , Células Cultivadas , Feto/citologia , Humanos , Interações Hidrofóbicas e Hidrofílicas , Osteoblastos/química , Engenharia Tecidual/métodosRESUMO
To control the selective adhesion of human endothelial cells and human serum proteins to bioceramics of different compositions, a multifunctional ligand containing a cyclic arginine-glycine-aspartate (RGD) peptide, a tetraethylene glycol spacer, and a gallate moiety was designed, synthesized, and characterized. The binding of this ligand to alumina-based, hydroxyapatite-based, and calcium phosphate-based bioceramics was demonstrated. The conjugation of this ligand to the bioceramics induced a decrease in the nonselective and integrin-selective binding of human serum proteins, whereas the binding and adhesion of human endothelial cells was enhanced, dependent on the particular bioceramics.