Alarming waterweeds proliferation in the Vembanad Lake System might significantly increase water loss through transpiration.

Eichhornia crassipes and Monochoria vaginalis are waterweeds, and their uncontrolled proliferation in fresh and brackish water habitats is a serious ecological problem in many parts of the world. These weeds are quite common in the Vembanad Lake System (VLS), India's second-largest Ramsar wetland. During the non-monsoon season, the Thannermukkom saltwater barrage divides the VLS into two zones: saline water downstream and freshwater upstream. The field sampling of the current study was carried out in the upstream zone of the VLS during the Pre-Monsoon (March 2017). Fresh Eichhornia and Monochoria samples were collected, transported to the lab, and experiments were conducted under natural light conditions to determine how much extra water they transpire into the atmosphere. The results showed that the water loss in experimental tanks with Eichhornia (evapotranspiration) is roughly twice that in control tanks without them (only evaporation). Monochoria transpires fairly more water to the atmosphere than Eichhornia. These results reveal that the proliferation of waterweeds has a significant adverse effect in conserving water in all freshwater bodies infested with them. The current study also points out that the expansion of waterweeds has the potential to worsen drought conditions as they cause excess water loss into the atmosphere and a faster drying up of freshwater reservoirs. Two possible approaches for managing the waterweeds in the VLS include reducing nutrient loading upstream and more frequent opening of the Thannermukkom saltwater barrage to allow saltwater intrusion, which could inhibit the growing waterweeds.

Dominance of Wolbachia sp. in the deep-sea sediment bacterial metataxonomic sequencing analysis in the Bay of Bengal, Indian Ocean.

The Bay of Bengal, located in the north-eastern part of the Indian Ocean is world's largest bay occupying an area of ~8,39,000 mile2. The variability in bacterial community structure and function in sediment ecosystems of the Bay of Bengal is examined by Illumina high-throughput metagenomic sequencing. Of five metataxonomics data sets presented, two (SD1 and SD2) were from stations close to the shore and three (SD4, SD5, and SD6) were from the deep-sea (~3000 m depth). Phylum Proteobacteria (90.27 to 92.52%) dominated the deep-sea samples, whereas phylum Firmicutes (65.35 to 90.98%) dominated the coastal samples. Comparative analysis showed that coastal and deep-sea sediments showed distinct microbial communities. Wolbachia species, belonging to class Alphaproteobacteria was the most dominant species in the deep-sea sediments. The gene functions of bacterial communities were predicted for deep-sea and coastal sediment ecosystems. The results indicated that deep-sea sediment bacterial communities were involved in metabolic activities like dehalogenation and sulphide oxidation.

Coccolithophores: an environmentally significant and understudied phytoplankton group in the Indian Ocean.

Coccolithophores are unique primary producers in the ocean with the ability to calcify. They are known to produce calcareous scales, which form the significant part of calcite oozes or chalk deposits on the seafloor. Coccolithophores are very noteworthy and they are explored to a great extent as nannofossils to reconstruct the past climate. Calcite plates in coccolithophores make them a vital tool in global climate change studies specifically with ocean acidification. These microscopic plants are the major contributor of the carbonate rain that controls the inorganic carbon pump in the ocean, which in turn influences both carbon and carbonate cycles. The emergence of advanced techniques enables us to study the biological aspects of this pelagic calcifier with improved precision. But still, they are understudied world over compared to any other phytoplankton groups. The northern Indian Ocean, being landlocked in three sides and vulnerable to climate change and ocean acidification, severely lacks focused studies on coccolithophores, though the US JGOFS in the 1990s have outlined the ecological significance of coccolithophores in the Arabian Sea. This paper reviews and outlines our understanding of coccolithophores as well as the nix in the northern Indian Ocean.

Impact of salinity on the grazing rate of a cladocera (Latonopsis australis) in a large tropical estuarine system.

Cladocerans are ecologically important as active grazers at the secondary trophic level, and they are economically important in aquaculture as potential live feed for many commercially valuable fishes. This study deals with the effect of salinity on grazing of the rare cladocera Latonopsis australis. The experimental specimens were collected from the lower reaches of the Kochi backwaters, the largest estuarine system along the west coast of India, during the Pre-Southwest Monsoon (May 2015), and their cultures developed in the laboratory. Eight experimental salinity treatments (freshwater, 2, 4, 6, 8, 10, 12 and 14 salinity) with three life phases of the specimens (phase I: neonates with a developed digestive tract, phase II: adults carrying egg and phase III: adults carrying developing neonates) were carried out to measure their grazing rates. Two different approaches, namely individual-specific and biovolume-specific grazing measurements, were adopted in the study. The results showed a significant influence of salinity on the size and grazing rates of L. australis, irrespective of their life phases. Filtration and ingestion rates of the specimens also varied significantly between different life phases with the lowest rates in phase III. Irrespective of the life phases, grazing rates were the highest in freshwater conditions, which decreased to 25-84% in medium salinity (4-8) and 65-93% in high salinity (10-14). Further, the study demonstrated that size/biovolume/weight-based quantification of cladocera grazing provides a more precise method for the estimation of feeding rates compared to the individual-basic approach. This study emerges as the first attempt to quantify the grazing measurements of a cladocera from Indian waters.

Role of rotifers in microzooplankton community in a large monsoonal estuary (Cochin backwaters) along the west coast of India.

The distribution ecology of microzooplankton in the Kochi (Cochin) backwaters has been presented. Emphasis has been given to the micro-rotifers present in the environment, considering they were a hitherto ignored component of the microzooplankton in the past studies. Three seasonal samplings were carried out at six locations along the salinity gradients in the Kochi backwaters during the Pre-Monsoon (March), Southwest Monsoon (August), and Northeast Monsoon (December). A total of 48 species of microzooplankton were recorded, of which 35 were ciliates, 10 were rotifers, and 3 were heterotrophic dinoflagellates. The study also reports the swarm of a microzooplankton species from the Kochi backwaters, which was formed by a tintinnid ciliate, Tintinnopsis uruguayensis, during the Northeast Monsoon. Very high microzooplankton density (11,990 No. L-1), as swarm in the downstream location was associated with the mesohaline condition and high availability of food. Rotifers were the major component of microzooplankton in the limnohaline/oligohaline region, whereas ciliates dominated in the polyhaline/mesohaline region. Hence, in the present study, salinity appeared to be a major factor affecting the composition of the microzooplankton community in the Kochi backwaters. As rotifers have a wide food spectrum, they can feed on almost all components of the microbial food web, including small ciliates. They also share the same food spectrum with larger ciliates and crustacean nauplii. The present study, for the first time, recorded the importance of rotifers in the microzooplankton community in the plankton food web in the Kochi backwaters.

Copepod grazing and their impact on phytoplankton standing stock and production in a tropical coastal water during the different seasons.

The grazing rate of copepods on the total and size-fractionated phytoplankton biomass in a coastal environment (off Kochi, southwest coast of India) were measured during pre-monsoon (PRM), peak southwest monsoon (PKSWM), late southwest monsoon (LSWM) and post-southwest monsoon (PSWM). The phytoplankton standing stock (chlorophyll a-Chl. a) and growth rate (GR) were less during the PRM (Chl. a 0.58 mg m-3; GR 0.23 ± 0.02) and PSWM (Chl. a 0.89 mg m-3; GR 0.30 ± 0.05) compared to PKSWM (Chl. a 6.67 mg m-3; GR 0.43 ± 0.02) and LSWM (Chl. a 4.09 mg m-3; GR 0.40 ± 0.04). The microplankton contribution to the total Chl. a was significant during the PKSWM (41.83%) and LSWM (45.72%). Copepod density was lesser during the PRM (1354 No m-3) and PSWM (1606 No m-3) than during PKSWM and LSWM (4571 and 3432 No m-3, respectively). Seasonal changes in phytoplankton biomass, phytoplankton size structure, and copepod community were closely related to the hydrographical transformations in the study domain. Dominant calanoid copepods in the study region ingested 8.4 to 14.2% of their daily ration from phytoplankton during the PRM and PSWM, which increased to >50% during the PKSWM and LSWM. The cyclopoid Oithona similis was abundant during the PKSWM, ingesting only 21% of their daily ration from phytoplankton. Temporal variation in the phytoplankton biomass and copepod species composition caused differences in community level top-down control. The copepod community ingestion on phytoplankton was high during the LSWM (18,583 µg C m-3d-1), followed by PKSWM (9050 µg C m-3d-1), PSWM (1813 µg C m-3d-1), and PRM (946 µg C m-3d-1). During the low Chl. a period (PRM and PSWM), dominant calanoid copepods showed a positive selectivity for the micro- and nano-phytoplankton size fractions, whereas during the high Chl. a period (PKSWM and LSWM), they showed a positive selection for nano-phytoplankton fractions. Irrespective of the seasons, dominant calanoid copepods showed a negative selection of pico-phytoplankton fraction. The cyclopoid O. similis and Poecilostomatoid Corycaeus danae showed a positive selection of nano- and pico-phytoplankton fractions rather than micro-fraction. The grazing pressure of copepod community ingestion on micro-fraction was less (0.56% of the phytoplankton biomass and 1.06% of the phytoplankton production) during the PKSWM. This study provides, for the first time, clear findings on the seasonal variation in the top-down control of phytoplankton by copepods in a tropical coastal water ecosystem and discusses its implications on phytoplankton blooming, plankton food web, and biogeochemistry.

Feeding preference and daily ration of 12 dominant copepods on mono and mixed diets of phytoplankton, rotifers, and detritus in a tropical coastal water.

Results of the experimental studies on the feeding habit and daily ration (DR) of 12 dominant copepods from a tropical coastal water (off Kochi, Southwest coast of India) on different food items (phytoplankton, rotifers, and detritus) are presented. Even though, all species of copepods consumed all types of food items in the experiments, they showed noticeable feeding preferences, having important ecological implications. Calanoid Paracalanus parvus and Acrocalanus gracilis consumed phytoplankton and rotifers equally in mono diets (74-89% of DR) and mixed diets (53-82% of DR), which indicated their ability to shift their diet in natural environment based on the availability of food items. Calanoid Acartia erythraea and A. danae consumed more phytoplankton (DR 83 and 72%, respectively) than rotifers (DR 51 and 46%, respectively) in mono diets, and in mixed diets, their consumption was high in phytoplankton combined food mixtures (P + R DR and P + D DR) rather than the R + D food type, indicated their preference for mixed diets of phytoplankton. Similarly, Calanoid Temora turbinata, Pseudodiaptomus serricaudatus, and Centropages tenuiremis preferred a herbivorous diet as evidenced by their high ingestion rate on phytoplankton mono (70 to 87% to their DR) and mixed diets (58 to 80% of DR). On the other hand, Cyclopoid Oithona similis and Poecilostomatoid Corycaeus danae preferred a carnivorous diet, consuming more rotifers (> 80% of DR) than phytoplankton (18-20% of DR) and detritus (5-6% of DR). Harpacticoids Macrosetella gracilis and Euterpina acutifrons equally preferred phytoplankton (78-92% of DR) and detritus (65-89% of DR). The study showed that the dominant copepods in the coastal waters off Kochi occupy different trophic niches available in the environment, which may be applicable in other similar environments as well.

Noctiluca and copepods grazing on the phytoplankton community in a nutrient-enriched coastal environment along the southwest coast of India.

The relative grazing impact of Noctiluca scintillans (hereafter referred only Noctiluca) and copepods (Acrocalanus gracilis, Paracalanus parvus, Acartia danae and Oithona similis) on the phytoplankton community in an upwelling-mudbank environment along the southwest coast India is presented here. This study was carried out during the Pre-Southwest Monsoon (April-May) to the Late Southwest Monsoon (August) period in 2014. During the sampling period, large hydrographical transformation was evident in the study area (off Alappuzha, Southwest coast of India); warmer Pre-Southwest Monsoon water column condition got transformed into cooler and nitrate-rich hypoxic waters during the Southwest Monsoon (June-August) due to intense coastal upwelling. Copepods were present in the study area throughout the sampling period with a noticeable increase in their abundance during the Southwest Monsoon. On the other hand, the first appearance of Noctiluca in the sampling location was during the Early Southwest Monsoon (mid-June) and thereafter their abundance increased towards the Peak Southwest Monsoon. The grazing experiments carried out as per the food removal method showed noticeable differences in the feeding preferences of Noctiluca and copepods, especially on the different size fractions of phytoplankton. Noctiluca showed the highest positive electivity for the phytoplankton micro-fraction (av. 0.49 ± 0.04), followed by nano-fraction (av. 0.17 ± 0.04) and a negative electivity for the pico-fraction (av. -0.66 ± 0.06). In total ingestion of Noctiluca, micro-fraction contribution (83.7%) was significantly higher compared to the nano- (15.7%) and pico-fractions (0.58%). On the other hand, copepods showed the highest positive electivity for the phytoplankton nano-fraction (av. 0.38 ± 0.04) followed by micro- (av. -0.17 ± 0.05) and pico-fractions (av. -0.35 ± 0.05). Similarly, in total ingestion of copepods, nano-fraction (69.7%) was the highest followed by micro- (28.9%) and pico-fractions (1.37%). The grazing pressure of Noctiluca on the total phytoplankton was found to be 27.7% of the standing stock and 45.6% of the production, whereas in the case of copepods, it was 9.95% of the standing stock and 16.6% of the production. The study showed that the grazing pressure of Noctiluca on the total phytoplankton as well as larger phytoplankton fraction was 2.8- and 8-folds higher than that of the copepods. This suggests the leading role of Noctiluca as an effective grazer of larger phytoplankton along the southwest west coast of India, especially during the Peak/Late Southwest Monsoon.

Tumour-like anomaly of copepods-an evaluation of the possible causes in Indian marine waters.

Globally, tumour-like anomalies (TLA) in copepods and the critical assessment of their possible causes are rare. The exact causative factor and ecological consequences of TLA in copepods are still unclear and there is no quantitative data available so far to prove conclusively the mechanism involved in developing TLA in copepods. TLA in copepods are considered as a potential threat to the well-being of the aquatic food web, which prompted us to assess these abnormalities in Indian marine waters and assess the possible etiological agents. We carried out a focused study on copepods collected from 10 estuarine inlets and five coastal waters of India using a FlowCAM, advanced microscopes and laboratory-incubated observations. The analysis confirmed the presence of TLA in copepods with varying percentage of incidence in different environments. TLA was recorded in 24 species of copepods, which constituted ~1-15 % of the community in different environments. TLA was encountered more frequently in dominant copepods and exhibited diverse morphology; ~60 % was round, dark and granular, whereas ~20 % was round/oval, transparent and non-granular. TLA was mostly found in the dorsal and lateral regions of the prosome of copepods. The three suggested reasons/assumptions about the causes of TLA such as ecto-parasitism (Ellobiopsis infection), endo-parasitism (Blastodinium infection) and epibiont infections (Zoothamnium and Acineta) were assessed in the present study. We did find infections of endo-parasite Blastodinium, ecto-parasite Ellobiopsis and epibiont Zoothamnium and Acineta in copepods, but these infectious percentages were found <1.5 % to the total density and most of them are species specific. Detailed microscopical observations of the samples collected and the results of the incubation experiments of infected copepods revealed that ecto-parasitism, endo-parasitism and epibiont infections have less relevance to the formation of TLA in copepods. On the other hand, these studies corroborated the view that wounds on the exoskeleton caused by partial predation as the potential reason for the TLA of copepods in Indian waters.

Summer monsoon onset-induced changes of autotrophic pico- and nanoplankton in the largest monsoonal estuary along the west coast of India.

This study presents the response of autotrophic pico- and nanoplankton to southwest monsoon-associated hydrographical transformations in the Cochin backwaters (CBW), the largest monsoonal estuary along the west coast of India. By the onset of the southwest monsoon, the euhaline/mesohaline conditions in the downstream/upstream of CBW usually transform into oligohaline/limnohaline. The flow cytometer analysis revealed the dominance of picoeukaryotes > Synechococcus > nanoautotrophs, with Prochlorococcus either very low or entirely absent. Synechococcus abundance was high during the pre-southwest monsoon (10(6) L(-1)), which dwindled with heavy fresh water influx during the southwest monsoon (10(5) L(-1)). The drastic drop in salinity and faster flushing of the CBW during the southwest monsoon replaced the euhaline/mesohaline strain of Synechococcus with an oligohaline/limnohaline strain. Epifluorescence microscopy analyses showed that, among the two strains of Synechococcus, the phycoerythrin-rich (PE-rich) one was dominant in the mesohaline/euhaline conditions, whereas the phycocyanin-rich (PC-rich) strain dominated in oligohaline/limnohaline conditions. Although Synechococcus abundance diminished during the southwest monsoon, the total abundance of picoplankton community remained virtually unchanged in the upstream due to an increase in the abundance of picoeukaryotes. On the other hand, the autotrophic nanoplankton abundance increased from pre-monsoon levels of av. 3.8 × 10(6)-av. 9.5 × 10(6) L(-1) at the onset of the southwest monsoon. Utilizing suitable multivariate analyses, the study illustrated the differential response and niche preference of various smaller communities of autotrophs to the southwest monsoon-associated hydrographical ramifications in a large monsoonal estuary, which may be applicable to similar such estuaries situated along the Indian coastline.

Copepod carcasses in a tropical estuary during different hydrographical settings.

Dead copepods (carcasses) are widespread in aquatic systems, but their scientific quantification is rare due to the difficulty in discriminating them from live ones. In this paper, we hypothesized that due to large spatial and temporal changes in hydrography in the Cochin backwaters, the percentage of copepod carcasses in the system could also change significantly on a spatial and temporal scale. In order to understand this aspect, we quantified the live and dead copepods in the Cochin backwaters under different hydrographical settings based on live and mortal staining technique. The most prominent temporal hydrographical feature during the study period was the large decline in salinity across the system, which was more pronounced downstream (15-20 units) and was caused by the large freshwater influx associated with the southwest monsoon. During the entire sampling period, copepod carcasses were pervasive all over the study area with large spatial and temporal variations in their percentage contribution (2.5-35.8 %) to the total community abundance. During all sampling, carcasses concentrated more in the downstream region, with maximum turbidity (16.5-35.8 %), than in the upstream region (2.5-14.5 %). The percentage of carcasses was the highest during the onset of the southwest monsoon (av. 23.64 ± 8.09 %), followed by the pre-southwest monsoon (av. 13.59 ± 6.72 %) and southwest monsoon (av. 8.75 ± 4.14 %). During the onset of the southwest monsoon, copepod carcasses in the downstream were contributed by ∼80 % high saline and ∼15 % low saline species, indicating a salinity shock-induced mortality. On the other hand, the cumulative effect of the long residence time of the Cochin backwaters and high partial predation rate of carnivores contributed to the high abundance of carcasses during the pre-monsoon.

Influence of salinity on the life table demography of a rare Cladocera Latonopsis australis.

Latonopsis australis is a rare Cladocera inhabiting the entire stretch of the Cochin backwaters, the largest monsoonal estuary along the West Coast of India, during the summer monsoon, but restricted to the upper reaches during the non-monsoon periods. Here, we present the results of an experimental study, which assessed the influence of salinity on the life table demography of the species at different salinity levels. The life table demographic parameters such as net reproduction rate, generation time, intrinsic growth rate, gross reproductive rate, and survivorship of the species were measured in different salinities ranging from freshwater to mesohaline levels (salinity 14). The study showed that higher salinity had a significant negative effect on all life table demography parameters of the species, whereas freshwater to low saline conditions (salinity up to 8) favored the survivorship, life expectancy, net production, and growth rate. It was also noticed that salinity above 8 caused a significant decrease in the survivorship, life expectancy, and reproduction rate of the species, which clearly explained the seasonal distribution pattern of the species in the Cochin backwaters. The present study suggests salinity 2 to 6 as the optimum range for the large-scale production of L. australis for purposes like live feed in aquaculture.

Plankton food web and its seasonal dynamics in a large monsoonal estuary (Cochin backwaters, India)-significance of mesohaline region.

The paper presents the ecology and dynamics of plankton food web in the Cochin backwaters (CBW), the largest monsoonal estuary along the west coast of India. The data source is a time series measurement carried out in the CBW during the Spring Intermonsoon (March-May) and the Southwest Monsoon (June-September). The plankton food web consisting of autotrophic/heterotrophic picoplankton, autotrophic/heterotrophic nanoplankton, microzooplankton, and mesozooplankton was quantified in relation to the seasonal hydrographical settings in the CBW. The study showed that significant changes in the abundance and dynamics of plankton food web components were governed mostly by the spatial and seasonal changes in hydrography rather than short-term changes induced by tide. During the Spring Intermonsoon, all plankton consumers in the CBW was higher than the Southwest Monsoon, and the trophic interaction was more effective in upstream where there was a close coupling between all prey components and their consumers. During the Southwest Monsoon, on the other hand, the trophic interaction was more effective downstream where the abundance of all plankton consumers was significantly higher than the upstream. Based on statistical analyses NMDS/SIMPROF and RDA, we demarcated the spatial difference/mismatch in the prey and consumer distribution in the CBW and showed that a more efficient plankton food web exists in the mesohaline regions during both seasons. This suggests that a noticeable spatial shift occurs seasonally in the active plankton food web zone in the CBW; it is upstream during the Spring Intermonsoon and downstream during the Southwest Monsoon.

Why do satellite imageries show exceptionally high chlorophyll in the Gulf of Mannar and the Palk Bay during the Norteast Monsoon?

The Gulf of Mannar (GoM) and the Palk Bay (PB) are two least studied marine environments located between India and Sri Lanka. Exceptionally high chlorophyll a concentration in the GoM and the PB during the Northeast Monsoon (November-February) is a consistent feature in satellite imageries, which has been attributed to the intrusion of the Bay of Bengal (BoB) waters. The analyses of the Moderate Resolution Imaging Spectroradiometer (MODIS) and field chlorophyll data collected from 30 locations in the Indian sector of the GoM and the PB in January 2011 showed significant overestimations in the satellite data. This error was much higher in the PB (60-80 %) as compared to the GoM (18-28 %). The multivariate analyses evidenced that the exceptionally high satellite chlorophyll in the PB is contributed largely by turbidity, colored dissolved organic matter (CDOM), and bottom reflectance. The paper cautions that though MODIS is superior in estimating chlorophyll a in optically complex waters, there are still chances of overestimations in regions like the PB.

Impact of river discharge and suspended sediments on the nearshore benthic environment along the Indian southwest coast.

River discharge into the sea and its implications on the environmental setting and fauna in the nearshore represent the intricate interactions among the atmosphere, hydrosphere, lithosphere, and biosphere. This study, based on in-situ and satellite data, presents how spatially varying river discharge laden with suspended sediments structure the hydrography and the nearshore benthic environment over a 590 km southwest (Kerala) coast of India. The 41 rivers that discharge along the Kerala coast are monsoon-driven; they are small but swift and cumulatively supply huge amounts of freshwater and suspended sediments into the Southeastern Arabian Sea (SEAS) during the Southwest Monsoon (SWM) when around 70 % (1925 mm) of the yearly rainfall occurs. These rivers are distributed unevenly along the coastline, with twenty-four of them discharging in the northern region (10.9-12.5°N), nine in the central (9.2-10.9°N), and eight in the south (8-9.2°N). During the SWM, plumes of low salinity (<32) with high clay content were widespread in the northern and central regions of the study area due to heavy river discharge and the presence of crystalline rocks and laterite deposits. In contrast, the low salinity plumes and suspended sediments were markedly low in the southern region due to limited river discharge and a predominance of sedimentary rocks and coastal alluvium that supported sandy sediments. This resulted in a spatial change in benthic sediment texture as well, with the central and northern regions more silty or clayey, while the south was sandy. Interestingly, the sandy south section had a noticeably higher faunal abundance than the rest of the region with an increased abundance of sensitive fauna of molluscs and echinoderms. This is a clear case of how spatially varying river discharge and sediment flux impact the nearshore environment and fauna.

Copepoda community imprints the continuity of the oceanic and shelf oxygen minimum zones along the west coast of India.

The largest continental shelf Oxygen Minimum Zone (OMZ) in the world is formed along the Indian western shelf in the eastern Arabian Sea during the Southwest Monsoon [(SWM); June-September], which is a natural pollution event associated with the coastal upwelling. This study examines the composition, abundance, and distribution of copepods during the Northeast Monsoon [(NEM); November to February] and SWM in 50 m depth zones along the Indian western shelf in the eastern Arabian Sea. The NEM was characterised by warm, stratified, and low-salinity waters in the southeast Arabian Sea and cold, high-salinity, and well-mixed waters in the northeastern Arabian Sea. During the SWM, cold and Dissolved Oxygen (DO) deficient waters (<22 µM/0.5 ml L-1), which are the signs of coastal upwelling, were evident all along the study zone, but with more intensity off Kochi, Mangalore, and Goa in the south than off Mumbai and Okha in the north. The zooplankton total biomass and abundance showed seasonality with a general decrease during the SWM (av. 3.68 ± 1.29 ml m-3 and av. 5711 ± 3096 Ind. m-3, respectively) compared to the NEM (av. 7.37 ± 2.17 ml m-3 and av. 14,473 ± 4966 Ind. m-3, respectively). At the same time, the abundance of Polychaeta and Siphonophora showed an increase during the SWM (av. 1187 ± 1055 Ind. m-3 and av. 169 ± 119 Ind. m-3, respectively), probably a result of the DO deficient waters associated with upwelling. Two striking seasonal features in Copepoda community were evident in this study: (a) a compositional shift from Cyclopoida dominant during the NEM to Calanoida dominant during the SWM, and (b) the coastal OMZ along the Indian western shelf during the SWM was dominated by Calanoida, which include oceanic OMZ species such as Pleuromamma indica, Lucicutia flavicornis, L.paraclausii, Eucalanus elongatus, Subeucalanus pileatus, S.subcrassus, and Clausocalanus furcatus. This forms a clear imprint for the extension of the oceanic OMZ into nearshore waters during the SWM due to coastal upwelling.

Impact of eutrophication on the occurrence of Trichodesmium in the Cochin backwaters, the largest estuary along the west coast of India.

Phytoplankton studies in early 1970s have shown the annual dominance of diatoms and a seasonal abundance of Trichodesmium in the lower reaches of the Cochin backwaters (CBW) and adjacent coastal Arabian Sea during the pre-summer monsoon period (February to May). Surprisingly, more recent literature shows a complete absence of Trichodesmium in the CBW after 1975 even though their seasonal occurrence in the adjacent coastal Arabian Sea continued without much change. In order to understand this important ecological feature, we analyzed the long-term water quality data (1965-2005) from the lower reaches of the CBW. The analyses have shown that salinity did not undergo any major change in the lower reaches over the years and values remained >30 throughout the period. In contrast, a tremendous increase was well marked in levels of nitrate (NO(3)) and phosphate (PO(4)) in the CBW after 1975 (av. 15 and 3.5 µM, respectively) compared with the period before (av. 2 and 0.9 µM, respectively). Monthly time series data collected in 2004-2005 period from the lower reaches of the CBW and coastal Arabian Sea have clearly shown that the physical characteristics like salinity, temperature, water column stability, and transparency in both regions are very similar during the pre-summer monsoon period. In contrast, the nutrient level in the CBW is several folds higher (NO(3), 8(;) PO(4), 4; SiO(4), 10; and NH(4), 19 µM) than the adjacent coastal Arabian Sea (NO(3), 0.7; PO(4), 0.5; SiO(4), 0.9; and NH(4), 0.6 µM). The historic and fresh time series data evidences a close coupling between enriched levels of nutrients and the absence of Trichodesmium in the Cochin backwaters.

Winter phytoplankton size classes in the Northeastern Arabian Sea based on in-situ and remote sensing methods.

Phytoplankton size classes (PSCs) are important in marine ecosystems because they organise the food chain and trophic pathways, which determine the overall biological environment. Based on three FORV Sagar Sampada cruises, the current study provides changes in PSCs in the Northeastern Arabian Sea (NEAS; north of 18 N) during different phases of the Northeast Monsoon [NEM (November-February)]. During all three phases of NEM such as early (November), peak (December), and late (February), in-situ chlorophyll-a fractionation data revealed that nanoplankton (2-20 µm) predominated, followed by microplankton (>20 µm) and picoplankton (0.2-2.0 µm). This was primarily because winter convective mixing in the NEAS maintains only a moderate level of nutrients in the surface mixed layer, which is more conducive to the dominance of nanoplankton. Brewin et al. (2012) and Sahay et al. (2017) have satellite-based PSC estimation algorithms; the former was developed for the entire Indian Ocean, while the latter is a modification of the former for the Noctiluca bloom-infested NEAS, with a claim that such blooms are typical of the NEM. When current in-situ PSCs data were compared to algorithm-based NEM data, Brewin et al. (2012) revealed a more realistic PSCs contribution pattern, especially in oceanic waters, with nanoplankton predominating except during early NEM. But the PSCs data from Sahay et al. (2017) showed a high degree of variation from the in-situ data, demonstrating the dominance of pico- and microplankton and a notably small contribution from the nano phytoplankton. The current study showed that Sahay et al. (2017) is inferior to Brewin et al. (2012) at quantifying PSCs in the NEAS without Noctiluca blooms, and provided evidence to show that Noctiluca blooms are not a typical feature of the region during the NEM.

Microplastics in copepods reflects the manmade flow restrictions in the Kochi backwaters, along the southwest coast of India.

This baseline study on microplastics (MPs) in calanoid copepods in the Kochi backwaters (KBW), India's largest estuary system on the west coast, focuses on (a) the spatiotemporal variations of MPs with the seasonal hydrography setting, and (b) how man-made flow restrictions of a large saltwater barrage contribute to MPs in copepods and their potential to transfer to higher trophic levels. This study found that MPs in copepods in the KBW ranged from av. 0.01 ± 0.014 to 0.11 ± 0.03 no./ind. seasonally. When the saltwater barrage shutters were fully/partially closed during the Pre-monsoon/Northeast Monsoon, MPs in copepods were considerably larger (av. 0.11 ± 0.03 no./ind., and av. 0.075 ± 0.02 no./ind., respectively) as compared to the Southwest Monsoon (av. 0.03 ± 0.01 no./ind.), when the barrage shutters were fully open. This shows the potential of man-made flow restrictions to increase the bioconcentration of MPs in copepods and their possible transfer to higher trophic levels through the food chain, adding to the region's previous discovery that much higher trophic level resources are polluted with a high concentration of MPs.

Microplastics in zooplankton in the eastern Arabian Sea: The threats they pose to fish and corals favoured by coastal currents.

The baseline study of Microplastics (MPs) in zooplankton (copepods, chaetognaths, decapods, and fish larvae) from six different zones along India's west coast (off Kanyakumari/Cape Comorin, Kochi, Mangalore, Goa, Mumbai, and Okha) in the Eastern Arabian Sea (EAS) is presented here with their vast ecosystem impacts. This investigation revealed that zooplankton in all six zones accumulated MPs pellets (52.14%), fibres (28.40%), films (10.51%), and fragments (8.95%). The highest average retention of MPs (MPs/individual) was found in fish larvae (av. 0.57 ± 0.18) while copepods had the lowest (av. 0.03 ± 0.01). The presence of low-density polyethylene, polypropylene, polystyrene, and polyethylene terephthalate was confirmed by Raman Spectra of MPs. The MPs in zooplankton found in this study (av. 22 ± 7 pieces/m3) were nearly 2-fold greater than those found in some of the world's most densely populated areas. It is shown that the strong southerly coastal currents could advect the MPs contaminated water mass too far away, having the potential to affect the fish and corals.