Golgi-Localized OsFPN1 is Involved in Co and Ni Transport and Their Detoxification in Rice.

Cobalt (Co) and nickel (Ni) are beneficial and essential elements for plants, respectively, with the latter required for urease activity, which hydrolyzes urea into ammonium in plants. However, excess Co and Ni are toxic to plants and their transport mechanisms in rice are unclear. Here, we analyzed an ethyl methanesulfonate (EMS)-mutagenized rice mutant, 1187_n, with increased Co and Ni contents in its brown rice and shoots. 1187_n has a mutation in OsFPN1, which was correlated with a high Co and Ni phenotype in F2 crosses between the parental line and mutant. In addition, CRISPR/Cas9 mutants exhibited a phenotype similar to that of 1187_n, demonstrating that OsFPN1 is the causal gene of the mutant. In addition to the high Co and Ni in brown rice and shoots, the mutant also exhibited high Co and Ni concentrations in the xylem sap, but low concentrations in the roots, suggesting that OsFPN1 is involved in the root-to-shoot translocation of Co and Ni. The growth of 1187_n and CRISPR/Cas9 lines were suppressed under high Co and Ni condition, indicating OsFPN1 is required for the normal growth under high Co and Ni. An OsFPN1-green fluorescent protein (GFP) fusion protein was localized to the Golgi apparatus. Yeast carrying GFP-OsFPN1 increased sensitivity to high Co contents and decreased Co and Ni accumulation. These results suggest that OsFPN1 can transport Co and Ni and is vital detoxification in rice.

Artificial chromosome technology and its potential application in plants.

Plant genetic engineering and transgenic technology are powerful ways to study the function of genes and improve crop yield and quality in the past few years. However, only a few genes could be transformed by most available genetic engineering and transgenic technologies, so changes still need to be made to meet the demands for high throughput studies, such as investigating the whole genetic pathway of crop traits and avoiding undesirable genes simultaneously in the next generation. Plant artificial chromosome (PAC) technology provides a carrier which allows us to assemble multiple and specific genes to produce a variety of products by minichromosome. However, PAC technology also have limitations that may hinder its further development and application. In this review, we will introduce the current state of PACs technology from PACs formation, factors on PACs formation, problems and potential solutions of PACs and exogenous gene(s) integration.

A simple and high-throughput method for xylem sap collection.

Xylem is the most important route for root-to-shoot translocation of water, nutrients and signaling molecules. Although a number of studies have been performed using xylem sap, its collection requires special equipment and is usually low throughput. Here, we developed a simple and high-throughput method for the collection of both medium- (<1 ml) and small- (<200 µl) volume xylem sap samples. Using a rice Cd transporter mutant, we demonstrated that our method allows for the effective evaluation of xylem sap Cd concentrations.

Adaption of Ulva pertusa to multiple-contamination of heavy metals and nutrients: Biological mechanism of outbreak of Ulva sp. green tide.

The multiple-contamination of heavy metals and nutrients worsens increasingly and Ulva sp. green tide occurs almost simultaneously. To reveal the biological mechanism for outbreak of the green tide, Ulva pertusa was exposed to seven-day-multiple-contamination. The relation between pH variation (VpH), Chl a content, ratio of (Chl a content)/(Chl b content) (Rchla/chlb), SOD activity of U. pertusa (ASOD) and contamination concentration is [Formula: see text] (p<0.05), Cchla=0.88±0.09-0.01±0.00×CCd (p<0.05), [Formula: see text] (p<0.05), and [Formula: see text] (p<0.05), respectively. Cammonia, CCd and CZn is concentration of ammonia, Cd2+ and Zn2+, respectively. Comparing the contamination concentrations of seawaters where Ulva sp. green tide occurred and the contamination concentrations set in the present work, U. pertusa can adapt to multiple-contaminations in these waters. Thus, the adaption to multiple-contamination may be one biological mechanism for the outbreak of Ulva sp. green tide.

Ocean acidification: One potential driver of phosphorus eutrophication.

Harmful algal blooms which may be limited by phosphorus outbreak increases currently and ocean acidification worsens presently, which implies that ocean acidification might lead to phosphorus eutrophication. To verify the hypothesis, oxic sediments were exposed to seawater with different pH 30days. If pH was 8.1 and 7.7, the total phosphorus (TP) content in sediments was 1.52±0.50 and 1.29±0.40mg/g. The inorganic phosphorus (IP) content in sediments exposed to seawater with pH8.1 and 7.7 was 1.39±0.10 and 1.06±0.20mg/g, respectively. The exchangeable phosphorus (Ex-P) content in sediments was 4.40±0.45 and 2.82±0.15µg/g, if seawater pH was 8.1 and 7.7. Ex-P and IP contents in oxic sediments were reduced by ocean acidification significantly (p<5%). The reduced phosphorus in sediments diffused into water, which implied that ocean acidification was one potential facilitator of phosphorus eutrophication in oxic conditions.

High Colonization Possibility of Some Species of Weeds in Suaeda salsa Community: From an Ecological Stoichiometry Perspective.

Suaeda salsa community is a vegetation type in saline-alkali areas. Weed invasion and colonization in S. salsa communities lead to fragmentationsof S. salsa communities. The colonization of invaded weeds in S. salsa communities is related to community succession of saline-alkali zones. The fragmented S. salsa community may be restored if the mechanism of invaded weed colonization in S. salsa communities is clearly elucidated. Thus, we studied the ecological stoichiometric characteristics of soils and plants in a salt marsh to explain the high colonization possibility of invaded weeds in S. salsa communities. In October 2014, soils and plants were collected from Dongfeng Salt Marsh, Jiaozhou Bay, Shandong Province, China. The ratio of Ex-N/Ex-P in soil was less than 13, which suggests a relative nitrogen limitation for the primary production in the zone. The minimum phosphorus content in plants was higher than 1 mg g-1, whereas the maximum nitrogen content in plants was less than 13 mg g-1. These results imply that phosphorus was abundant, whereas nitrogen was deficient in the area. The plants in the salt marsh may be limited by nitrogen. Given the relatively lower nitrogen contents in Cyperus glomeratus, Echinochloa crusgalli, and Aster subulatus than that in S. salsa, these three species exhibited higher competitiveness than S. salsa did when nitrogen was limited in primary production. These weed species may colonize highly in S. salsa communities. Moreover, nitrogen fertilization might be effective to maintain S. salsa community in Dongfeng Salt Marsh, whereas its effects on controlling weeds colonization in S. salsa communities need more studies to verify.