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Virus removal can be successfully achieved based on an electrostatic adsorption mechanism. The key requirement for this process is to develop filter materials that can be produced by low-cost technologies and are suitable in large-scale production for real applications. In this study, we report development of spray-dried alumina granules modified with copper (oxide) nanoparticles and critically assess the effect of copper oxidation state on virus removal capacity. Using plate-shaped alumina as a support material resulted in porous structure, which in turn ensured prolonged contact time of contaminated water with the material. Subsequently, copper (oxide) nanoparticles provided a large number of adsorption sites. Flow experiments revealed that copper(I) oxide and metallic copper were the active phases in virus removal and 99.9% of MS2 bacteriophages could be removed. However, almost no virus removal was observed in the presence of copper(II) oxide. Contrasting virus removal characteristics are associated with the different surface charge of copper species, as determined by zeta potential measurements.
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Poluentes Químicos da Água , Purificação da Água , Adsorção , Óxido de Alumínio , Cobre , Filtração , ÁguaRESUMO
Copy number variations (CNV) in CEBPA locus represent heterogeneous group of mutations accompanying acute myeloid leukemia (AML). The aim of this study was to characterize different CEBPA mutation categories in regard to biological data like age, cytology, CD7, and molecular markers, and identify possible factors affecting their etiology. We report here the incidence of 12.6% of CEBPA mutants in the population of 262 normal karyotype AML (NK-AML) patients. We confirmed that double mutant AMLs presented uniform biological features when compared to single CEBPA mutations and accompanied mostly younger patients. We hypothesized that pathogenesis of distinct CEBPA mutation categories might be influenced by different factors. The detailed sequence analysis revealed frequent breakpoint-associated microhomologies of 2 to 12bp. The analysis of distribution of microhomology motifs along CEBPA gene showed that longer stretches of microhomology at the mutational junctions were relatively rare by chance which suggests their functional role in the CEBPA mutagenesis. Additionally, accurate quantification of CEBPA transcript levels showed that double CEBPA mutations correlated with high-level CEBPA expression, whereas single N-terminal CEBPA mutations were associated with low-level CEBPA expression. This might suggest that high-level CEBPA expression and/or accessibility of CEBPA locus contribute to B-ZIP in-frame duplications.
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Proteínas Estimuladoras de Ligação a CCAAT/genética , Variações do Número de Cópias de DNA , Cariótipo , Leucemia Mieloide Aguda/genética , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Sequência de Bases , Cromatina/genética , Pontos de Quebra do Cromossomo , Biologia Computacional/métodos , Análise Mutacional de DNA , Feminino , Regulação Leucêmica da Expressão Gênica , Loci Gênicos , Humanos , Leucemia Mieloide Aguda/diagnóstico , Masculino , Pessoa de Meia-Idade , Mutagênese , Mutação , Motivos de Nucleotídeos , RNA Mensageiro/genética , Adulto JovemRESUMO
BACKGROUND: CA 19-9 is a commonly assessed tumor marker, considered characteristic of pancreatic ductal adenocarcinoma (PDAC) and biliary tract cancers; however, the positive predictive value of CA 19.9 is too low, and the usage of CA 19.9 as a screening tool in the healthy population remains controversial. METHODS: The presented case illustrates a reversed diagnosis of highly elevated serum CA 19-9 levels in a 54-year-old female complaining of pain in the epigastric region, shortly after COVID-19 vaccination. Laboratory tests showed a significantly elevated level of the CA 19-9 marker (>12,000 U/mL, reference value: <37 U/mL) with normal pancreatic enzyme activity. The patient underwent imaging examination, which showed no abnormalities, except for increased pancreatic dimension and areas of fluid signal in the pancreas in magnetic resonance imaging (MRI), which may correspond to autoimmune pancreatitis (AIP). The patient remains asymptomatic with a recommendation for a follow-up MRI in 12 months. RESULTS: A literature review conducted revealed multi-causal CA 19-9 increases above 1000 U/mL, including non-cancerous diseases of the lung, pancreas, liver, ovary, kidney, and others. The median concentration of CA 19-9 regardless of the cause of disease was 2810 U/mL (IQR ± 6895). The median CA 19-9 values in men and women were 3500 (IQR ± 10,050) and 2455 (IQR ± 3927), respectively, and differ significantly between the compared groups (p < 0.05). There was no difference between CA 19-9 values and the categorized cause of the increase. CONCLUSIONS: Conducting differential diagnosis, it should not be forgotten that most international guidelines recommend the use of CA 19-9 only in conjunction with pathology of pancreas in radiological imaging; however, even such a combination can point the diagnostic pathway in the wrong direction. A highly elevated CA 19-9 level, typically associated with PDAC, may be the result of benign disease including AIP related to COVID-19 vaccination.
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MXenes are a family of two-dimensional nanomaterials. Titanium carbide MXene (Ti3C2Tx-MXene), reported in 2011, is the first inorganic compound reported among the MXene family. In the present work, we report on the study of the composition and various physical properties of Ti3C2Tx-MXene nanomaterial, as well as their temperature evolution, to consider MXenes for space applications. X-ray diffraction, thermal analysis and mass spectroscopy measurements confirmed the structure and terminating groups of the MXene surface, revealing a predominant single OH layer character. The temperature dependence of the specific heat shows a Debye-like character in the measured range of 2 K-300 K with a linear part below 10 K, characteristic of conduction electrons of metallic materials. The electron density of states (DOS) calculations for Ti3C2OH-MXene reveal a significant DOS value at the Fermi level, with a large slope, confirming its metallic character, which is consistent with the experimental findings. The temperature dependence of electrical resistivity of the MXene samples was tested for a wide temperature range (3 K-350 K) and shows a decrease on lowering temperature with an upturn at low temperatures, where negative magnetoresistance is observed. The magnetoresistance versus field is approximately linear and increases its magnitude with decreasing temperature. The magnetization curves are straight lines with temperature-independent positive slopes, indicating Pauli paramagnetism due to conduction electrons.
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Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Duplicação Gênica , Cariótipo , Leucemia Mieloide Aguda/tratamento farmacológico , Leucemia Mieloide Aguda/genética , Tirosina Quinase 3 Semelhante a fms/genética , Cladribina/administração & dosagem , Citarabina/administração & dosagem , Daunorrubicina/administração & dosagem , Humanos , Leucemia Mieloide Aguda/mortalidade , Resultado do TratamentoRESUMO
Alumina ceramics were joined by a transient liquid phase (TLP) bonding method at relatively lower temperatures, using mixed powders of Bi2O3 and ZnO with different weight ratios as interlayers between the ceramic components. Bonding was achieved at 750 °C for several of the prepared interlayer mixtures, which makes the applied approach attractive due to the relatively lower joining temperature and potentially low fabrication costs. Measurements by SEM and EDX were used to study the microstructure and chemical analysis of the obtained joints. It also allowed us to investigate the diffusion mechanism occurring in the systems, which resulted in the hypothesis that Zn2+/ZnO diffuses through the ceramics. XRD and Raman spectra were acquired to examine the reaction products that formed during the thermal treatment. The results showed that both ZnO and Bi2O3 react with each other as well as with alumina to form spinel and other products.
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High-entropy materials are compositionally complex materials which often contain five or more elements. The most commonly studied materials in this field are alloys and oxides, where their composition allows for tunable materials properties. High-entropy layered double hydroxides have been recently touted as the next focus for the field of high-entropy materials to expand into. However, most previous work on multi-cationic layered double hydroxides has focused on syntheses with 5 or less cations in the structure. To bridge this gap into high-entropy materials, this work explores the range and extent of different compositional combinations for high-entropy double layered hydroxides. Specifically, pure layered double hydroxides were synthesized with different combinations of 7 cations (Mg, Co, Cu, Zn, Ni, Al, Fe, Cr) as well as one combination of 8 cations by utilizing a hydrothermal synthesis method. Furthermore, magnetic properties of the 8-cation LDH were investigated.
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Steroid-refractory acute graft-versus-host disease (SR-aGVHD) remains a major cause of morbidity and mortality after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Ruxolitinib (RUX), an oral JAK1 and JAK2 inhibitor, has recently been approved for patients with SR-aGVHD. The aim of this study was to evaluate RUX efficacy and toxicity in a real-world setting. Eighteen patients received RUX at 5 mg or 10 mg twice a day after a median 3 lines of prior unsuccessful immunosuppressive therapy. Median time on RUX therapy was 28 days (range 7-129). Five patients (28%) responded to RUX, including 4 complete responses and 1 partial response. Response to RUX was irrespective of aGVHD grade and the number of involved organs. One-year overall survival (OS) was 60% for RUX-responders versus 31% for non-responders (p = ns). Treatment duration greater than 29.5 days was found to have a positive impact on OS (p < 0.007). Major adverse events during RUX treatment were grade 3-4 thrombocytopenia (61% of patients) and cytomegalovirus reactivation (50%). After median follow-up of 55 days (range 29-706), 14 patients (78%) died, mainly due to further progression of GVHD. RUX may represent a valuable therapeutic option for some patients with advanced SR-aGVHD, but more studies are warranted.
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Doença Enxerto-Hospedeiro , Transplante de Células-Tronco Hematopoéticas , Humanos , Doença Enxerto-Hospedeiro/tratamento farmacológico , Doença Enxerto-Hospedeiro/etiologia , Pirimidinas/uso terapêutico , Pirazóis/efeitos adversos , Nitrilas/uso terapêutico , Transplante de Células-Tronco Hematopoéticas/efeitos adversos , Esteroides/uso terapêutico , Doença Aguda , Estudos RetrospectivosRESUMO
The study focuses on obtaining Inconel 625-NbC composites for high-temperature applications, e.g., jet engines, waste-to-energy combusting systems or gas engine turbines, and characterizing them in terms of their microstructure and hardness improvement. Synthesis was performed utilizing Spark Plasma Sintering (SPS) at 1150 °C under the load of 45 MPa in medium vacuum (under 10-3 MPa) for a total time of 60 min. Four sets of samples with different Inconel 625 to NbC weight ratios were prepared (5, 10, 20, and 30 wt.%), followed by a reference sample containing no ceramic reinforcement. Obtained materials were hot-rolled at 1150 °C with a 10% reduction step and later cut and polished to perform characterization utilizing scanning electron microscopy (SEM) equipped with energy dispersive spectroscopy (EDS) module and microhardness testing device equipped with Vickers indenter. Hardness was improved proportionally to NbC addition achieving an increase of up to 20% of reference values. Additional heat treatment was conducted on the hot-rolled samples at 1200 °C in an argon atmosphere to further observe the interaction between reinforcement and alloy. Their microstructure revealed the coarsening of precipitates within the metal matrix and partial reinforcement dissolution, which proved to be crucial to obtaining the highest quality composites with homogenous hardness improvement.
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Activated carbon (AC) exhibits superior sorption properties compared to other porous materials, due to well-developed porous structures and high surface areas. Therefore, it is widely applied in its various forms in water purification to remove a diverse range of contaminating species. The presence of viruses in fresh water bodies poses a serious issue for human health. However, AC has not yet been commonly applied to waterborne virus removal. In this study, we present oxidation and copper impregnation treatment procedures of activated carbon fibers (ACFs) that resulted in porous structure and surface chemistry modifications. The effect of these modifications on virus removal was investigated by experimental flow studies and revealed up to 2.8 log10 reduction value (LRV) and 3.6 LRV of MS2 bacterio-phages for non-modified and oxidized ACFs, respectively, emphasizing the advantages of ACF surface functionalization. Copper modified fibers demonstrated a high sensitivity to media composition, resulting in a release of metal and therefore limited virucidal capacity.
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Mutations in isocitrate dehydrogenase 1 and 2 (IDH1/2) genes occur in about 20% patients with acute myeloid leukemia (AML), leading to DNA hypermethylation and epigenetic deregulation. We assessed the prognostic significance of IDH1/2 mutations (IDH1/2+) in 398 AML patients with normal karyotype (NK-AML), treated with daunorubicine + cytarabine (DA), DA + cladribine (DAC), or DA + fludarabine. IDH2 mutation was an independent favorable prognostic factor for 4-year overall survival (OS) in total NK-AML population (p = 0.03, censoring at allotransplant). We next evaluated the effect of addition of cladribine to induction regimen on the patients' outcome according to IDH1/2 mutation status. In DAC group, 4-year OS was increased in IDH2+ patients, compared to IDH-wild type group (54% vs 33%; p = 0.0087, censoring at allotransplant), while no difference was observed for DA-treated subjects. In multivariate analysis, DAC independently improved the survival of IDH2+ patients (HR = 0.6 [0.37-0.93]; p = 0.024; censored at transplant), indicating that this group specifically benefits from cladribine-containing therapy. In AML cells with R140Q or R172K IDH2 mutations, cladribine restrained mutations-related DNA hypermethylation. Altogether, DAC regimen produces better outcomes in IDH2+ NK-AML patients than DA, and this likely results from the hypomethylating activity of cladribine. Our observations warrant further investigations of induction protocols combining cladribine with IDH1/2 inhibitors in IDH2-mutant.
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Antineoplásicos/uso terapêutico , Protocolos de Quimioterapia Combinada Antineoplásica , Isocitrato Desidrogenase/genética , Leucemia Mieloide Aguda/genética , Adolescente , Adulto , Idoso , Cladribina/uso terapêutico , Citarabina/uso terapêutico , Daunorrubicina/uso terapêutico , Humanos , Leucemia Mieloide Aguda/tratamento farmacológico , Leucemia Mieloide Aguda/mortalidade , Pessoa de Meia-Idade , Variantes Farmacogenômicos , Polônia/epidemiologia , Ensaios Clínicos Controlados Aleatórios como Assunto , Estudos Retrospectivos , Adulto JovemRESUMO
Kikuchi-Fujimoto disease (KFD), also known as histiocytic necrotizing lymphadenitis, is a benign and self-limiting disease typically characterized by the enlargement of regional lymph nodes and accompanied by fever. KFD affects predominantly young adult females of Asian origin and is rarely seen in European countries, where it may cause diagnostic difficulties. Kimura disease is a rare and benign chronic inflammatory soft tissue disorder of unknown origin, characterized by a triad of painless subcutaneous masses in the head or neck region accompanied by regional lymphadenopathy, blood and tissue eosinophilia, and markedly elevated serum immunoglobulin E levels. Although most cases of Kimura disease have originated in China, Japan or Southeast Asia, there have been sporadic case reports from Europe and America. Herein, we review in detail the clinical presentations, complications and current concepts in the pathogenesis, diagnosis and treatment of these diseases.
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Hiperplasia Angiolinfoide com Eosinofilia/complicações , Linfadenite Histiocítica Necrosante/complicações , Linfonodos/patologia , Hiperplasia Angiolinfoide com Eosinofilia/diagnóstico , Diagnóstico Diferencial , Linfadenite Histiocítica Necrosante/diagnóstico , Humanos , Doenças Linfáticas/diagnóstico , Doenças Linfáticas/etiologia , PescoçoRESUMO
Sarcoidosis and its aetiopathogenesis, epidemiology and diagnostic procedures (including the Kveim reaction) are presented in this paper. The clinical manifestations of this disease, especially in otolaryngological organs, including the larynx, salivary glands, nose and paranasal sinuses, are described. Treatment procedures, including surgical interventions and prognosis, are also discussed.
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Otorrinolaringopatias/epidemiologia , Sarcoidose/complicações , Sarcoidose/diagnóstico , Humanos , Otorrinolaringopatias/diagnóstico , Otorrinolaringopatias/terapia , Prognóstico , Sarcoidose/terapiaRESUMO
In this study, an Inconel 625-tungsten carbide (WC) composite system was investigated by means of microstructure changes affected by both heating rate and WC content. In order to investigate how the system behaves while exposed to fast thermal processing, controlled melting using a differential thermal analysis (DTA) apparatus was performed on the powders. Two WC powders with different average grain size were used to obtain six compositions of Inconel 625-WC powder mixtures (10, 20, and 30 wt.% WC). They were analyzed under 10 and 30 °C/min heating rate in order to obtain composite samples. Results from DTA together with SEM/energy-dispersive X-ray spectroscopy (EDS) microstructural observations allowed observing material changes during solidification. Because of the extensive microsegregation of alloying elements to liquid and their reactions with C, which derived from dissolved WC, the formation of secondary phases with improved microhardness was possible. The collected results provide a better understanding of material behavior during intensive thermal processing which can be useful when designing materials for the laser additive manufacturing technique.
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In this work, three-dimensional (3D) shaping of aluminum nitride (AlN) UV-curable dispersions using CeraFab 7500 device equipped with the light engine emitting 365 nm wavelength (a UV-LCM device) is presented. The purpose of this study was the shaping of AlN pieces with microchannels for the future potential use as microchannel heat exchangers. The dispersions were characterized by the means of the particle size distribution, rheological measurements, and the cure depth evaluation. In shaping via UV-LCM, we applied dispersions containing 40 vol % solid load and different types of photoinitiators and their concentrations, as well as different settings of the printing parameters. Cuboidal plates with channels and cylindrical 3D structures were fabricated, debound, and sintered. For comparing ceramics properties, reference samples were prepared via uniaxial and cold isostatic pressing, using the same powder mixture as in the dispersions, and later sintered. The thermal conductivity of the sintered specimens was calculated, based on density and thermal diffusivity measurements.
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Analysis of dense Potassium Sodium Niobate (KNN) ceramic obtained by hot pressing (HP) method at 1100 °C are presented in this paper. The synthesis of KNN-based piezoelectrics meets the following challenges-low density of material, uncontrolled K/Na ratio, multiphase composition and formation of different KNN structures. The classical hot pressing approach results in contamination by carbon originating from graphite molds. The proposed hexagonal Boron Carbide (h-BN) layer between green sample and graphite mold could protect samples from carbon contamination. Additionally, the presence of h-BN may decrease the formation of oxygen vacancies, which allows us to maintain the semiconductor features of the KNN structure. Remaining issues were addressed with the addition of excess Na and Er2O3 doping. The results showed that excess Na addition allowed us to compensate evaporation of sodium during the synthesis and sintering. Er2O3 was added as sintering aid to limit abnormal grain growth caused by h-BN addition. The modification of amount of Na and Er2O3 addition resulted in high purity KNN samples with tetragonal structure and apparent density higher than 97%. Finally, piezoelectric features of prepared dense samples were measured and presented.
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Energy harvesting from mechanical vibration of buildings is usually realized by the use of devices, in which the main element is a prismatic beam with a rectangular cross-section. The beam has been the subject of scientific research; it is usually constructed with a carrying substrate that does not have piezoelectric characteristics and from piezoelectric material. In contrast, this investigation sought to create a beam structure with a piezoelectric composite only. The entire beam structure was made of a prototype piezoelectric particulate composite. Based on courses of voltage obtained in laboratory experiments and known geometry of the specimens, a series of finite element method (FEM) simulations was performed, aiming to estimate the piezoelectric coefficient d31 value at which the mentioned voltage could be achieved. In each specimen, sedimentation caused the formation of two distinct layers: top and bottom. The experiments revealed that the presented prototype piezoelectric particulate composite converts mechanical stress to electric energy in bending mode, which is used in energy harvesting from mechanical vibration. It is self-supporting and thus a carrying substrate is not required in the harvester structure.
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In this study, the influence of the addition of rare earth oxides on the phase composition and density of KNN piezoelectric ceramics was investigated. The initial powders of Na2CO3 and K2CO3 were dried at 150 °C for 2 h. Then, a powder mixture for synthesis was prepared by adding a stoichiometric amount of Nb2O5 and 5 and 10 wt % overabundance of Na2CO3. All powders were mixed by ball-milling for 24 h and synthesized at 950 °C. The phase composition of the reaction bed was checked by means of X-ray diffraction (XRD). It had an appearance of tetragonal and monoclinic K0.5Na0.5NbO3 (KNN) phases. Then, 1 and 2 wt % of Er2O3 and Yb2O3, were added to the mixture. Green samples of 25 mm diameter and 3 mm thickness were prepared and sintered by hot pressing at 1000 °C for 2 h under 25 MPa pressure. The final samples were investigated via scanning electron microscopy (SEM)-energy-dispersive X-ray spectroscopy (EDS), XRD, Rietveld, and ultrasonic methods. Phase analysis showed tetragonal and orthorhombic KNN phases, and a contamination of (K2CO3·1.5H2O) was present. The obtained KNN polycrystals had a relative density above 95%. Texturing of the material was confirmed as a result of hot pressing.
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In this study, the laser metal deposition (LMD) of the Inconel 625â»tungsten carbide (WC) metal matrix composite was investigated. The composite coating was deposited on Inconel 625 substrate by powder method. A powder mixture containing 10 wt% of WC (5 µm) was prepared by wet mixing with dextrin binder. Coating samples obtained by low-power LMD were pore- and crack-free. Ceramic reinforcement was distributed homogenously in the whole volume of the material. Topologically close-packed (TCP) phases were formed at grain boundaries between WC and Inconel 625 matrix as a result of partial dissolution of WC in a nickel-based alloy. Line analysis of the elements revealed very small interference of the coating in the substrate material when compared to conventional coating methods. The average Vickers hardness of the coating was about 25% higher than the hardness of pure Inconel 625 reference samples.
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The paper presents the methodology and results of the ultrasound determination of material constants of anisotropic materials belonging to the transversely isotropic system. Ultrasound through-transmission method was used for determining material constants. Based on the measurements of velocities of longitudinal and transverse ultrasounds waves propagation, respectively polarized in required directions all the elastic and the material constant of the test materials were determined. Measurements of all the velocities necessary to determine the elastic constants were performed on a specially prepared individual samples. The tests were carried out on porous polycrystalline anisotropic graphites of anisotropy in Young's modulus of up to 26% and Al2O3 composites with up to 30% of hBN causing anisotropy of Young's modulus of up to 50%. It was found that for all tested samples the value of Young's modules and modules stiffness decreasing with increasing porosity in the graphites and increasing content of hBN in Al2O3.