RESUMO
The Type III secretion system is essential for intracellular replication of Edwardsiella tarda in phagocytes of fish and mammals. We identified the secreted proteins of the Type III secretion system by comparing the wild-type strain and the Type III mutant mET1229. The wild-type strain secreted 55, 25, and 22 kDa proteins into the culture supernatant, whereas the Type III mutant did not. These proteins were identified as EseB, EseC, and EseD and are similar in sequence to Salmonella SseB, SseC, and SseD that function as a translocon. The EseB, EseC, and EseD knockout mutants did not replicate in murine macrophages, suggesting that these proteins are essential for intracellular replication of E. tarda. Highest secretion of EseBCD proteins was observed when bacterial cells were cultured in neutral and alkaline pHs but not in acidic pH. When the pH of the phagosomes was examined using an acidotropic probe, the phagosomes containing the wild-type strain showed neutral pH, whereas those containing the Type III mutant exhibited acidic pH. These results suggest that the Type III-dependent interference with formation of the acidic environment in phagosomes is essential for intracellular replication of bacteria in murine macrophages.
Assuntos
Proteínas de Bactérias/metabolismo , Edwardsiella tarda/metabolismo , Macrófagos/microbiologia , Complexos Multiproteicos/metabolismo , Animais , Linhagem Celular , Regulação Bacteriana da Expressão Gênica/fisiologia , Concentração de Íons de Hidrogênio , Camundongos , Fagossomos , Transporte ProteicoRESUMO
OBJECTIVE: We investigated whether neural stem cells (NSC) with transgenic expression of human nerve growth factor (hNGF) transplanted into the brain could offer a therapeutic option for the treatment of Alzheimer's disease (AD). METHODS: We infused okadaic acid into rat lateral ventricles to establish a chronic AD animal model. In addition, NSC were stably transduced with hNGF and enhanced green fluorescent protein (eGFP) genes (NSC-hNGF-eGFP) by using a recombination adeno-associated virus serotype 2 (rAAV2) vector. These genetically modified stem cells were grafted into the cerebral cortex of AD rats. RESULTS: AD model rats showed significant damage in learning and memory function, with the formation of senile plaques and neurofibrillary tangles in the cerebral cortex. The transferred hNGF gene conferred stable and high levels of protein expression in NSC in vitro. Moreover, the NSC-hNGF-eGFP, but not the NSC, survived, integrating into the host brain and enhancing cognitive performance after transplantation. CONCLUSION: The injection of okadaic acid into rat lateral ventricles constitutes a promising animal model for investigating selective aspects of AD. rAAV2-mediated hNGF delivery can render long-term and stable transduction of hNGF in NSC. NSC-hNGF-eGFP transplantation may offer a viable therapeutic approach for treatment of AD.
Assuntos
Doença de Alzheimer/psicologia , Doença de Alzheimer/terapia , Fator de Crescimento Neural/genética , Transplante de Células-Tronco , Doença de Alzheimer/induzido quimicamente , Animais , Dependovirus , Modelos Animais de Doenças , Feto , Vetores Genéticos , Humanos , Aprendizagem , Masculino , Fator de Crescimento Neural/biossíntese , Neurônios/metabolismo , Ácido Okadáico/efeitos adversos , Ratos , Proteínas Recombinantes/genética , Células-Tronco/metabolismo , Transdução GenéticaRESUMO
In this study we examined the effect of the specific gravity of culture medium on the frequency of hematopoietic stem cell (HSC) maintenance. We used a newly developed high-specific-gravity media. Bone marrow cells were isolated and cultured, and HSC activity was evaluated. The number of hematopoietic progenitor/stem cells was markedly higher in the medium with high specific gravity. In high-specific-gravity media, cells did not precipitate, maintenance of HSCs was increased, and there was a concomitant accumulation of beta-catenin. This novel technique for maintaining HSC populations provides an important new tool for studies in regenerative medicine.
Assuntos
Técnicas de Cultura de Células/métodos , Células-Tronco Hematopoéticas/citologia , Animais , Transplante de Medula Óssea , Contagem de Células , Proliferação de Células , Meios de Cultura , Células-Tronco Hematopoéticas/metabolismo , Camundongos , Fatores de Tempo , beta Catenina/metabolismoRESUMO
The wooden model of the human skeleton, called the wooden skeleton, is a distinguished original craft object from the Edo era, in Japan, when medical doctors were unable to keep a human skeleton for study and teaching purposes. There are three types of wooden skeletons: (i) Hoshino made in 1792; (ii) Kagami made by 1810; and (iii) Okuda made around 1820. The former two are of adult males and the latter is of a female. The wooden skeletons were made with surprising accuracy compared with figures that appeared in the medical books available in Japan at that time, which suggests a scientific readiness of the doctors and the skill of the craftsmen. In the cases of the Hoshino and Kagami wooden skeletons, it is hard to consider that all wooden bones were assembled to show the entire body. Conversely, the Okuda wooden skeletons were made for showing in the sitting position. The skull of the Hoshino wooden skeleton is of special interest: the skull cap was not cut, yet the internal structures of the skull, such as the sella turcica, foramina for nerves and vessels, and the sulci for venous sinuses, were made with considerable accuracy. The skull caps of the Kagami and Okuda wooden skeletons were cut, as those used in modern medical education.
Assuntos
Anatomia/história , Esqueleto , História do Século XVIII , Humanos , Japão , MadeiraRESUMO
A three-dimensional (3D) clinostat is a device for generating multidirectional G force, resulting in an environment with an average of 10(3) G. Here we report that human mesenchymal stem cells (hMSCs) cultured in a 3D-clinostat (group CL) showed marked proliferation (13-fold in a week) compared with cells cultured under normal conditions of 1 G (group C) (4-fold in a week). Flow cytometry revealed a 6-fold increase in the number of hMSCs double-positive for CD44/CD29 or CD90/CD29 in group CL after 7 days in culture, compared with group C. Telomere length remained the same in cells from both groups during culturing. Group C cells showed increasing expression levels of type II collagen and aggrecan over the culture period, whereas group CL cells showed a decrease to undetectable levels. Pellets of hMSCs from each group were explanted into cartilagedefective mice. The transplants from group CL formed hyaline cartilage after 7 days, whereas the transplants from group C formed only noncartilage tissue containing a small number of cells. These results show that hMSCs cultured in a 3D-clinostat possess the strong proliferative characteristic of stem cells and retain their ability to differentiate into hyaline cartilage after transplantation. On the contrary, cells cultured in a 1-G environment do not maintain these features. Simulated microgravity may thus provide an environment to successfully expand stem cell populations in vitro without culture supplements that can adversely affect stem cell-derived transplantations. This method has significant potential for regenerative medicine and developmental biology.
Assuntos
Diferenciação Celular/fisiologia , Divisão Celular/fisiologia , Células-Tronco Mesenquimais/citologia , Ausência de Peso , Técnicas de Cultura de Células/instrumentação , Técnicas de Cultura de Células/métodos , Citometria de Fluxo , Humanos , CinéticaRESUMO
The wooden model of the human skeleton, called wooden skeleton, is a distinguished original craft object in Edo era (1600-1867), Japan, when medical doctors were unable to keep the human skeleton for their study and teaching purpose. There are three kinds of wooden skeletons, i. e. Hoshino, Kagami and Okuda wooden skeletons made in 1792, 1810 and 1820, respectively. The former two are of adult male and the latter of female. They were made in surprising accuracy as compared with figures appeared in medical books available in Japan at that time, which suggests scientific readiness of the doctors and skills of the craftsmen. A complete set of the skeleton, except for the hyoid bone, has been preserved for Hoshino and Okuda wooden skeletons, while several bones have been missing in Kagami wooden skeleton. Each bone of Hoshino and Kagami wooden skeletons was made separately and connected by a tenon and a corresponding mortise at the articular surface. So it is hardly considered that all wooden bones were assembled into the whole body skeleton on use. Okuda wooden skeleton, on the other hand, was made for being shown in sitting position. The skull of Hoshino wooden skeleton is of special interest: the skull cap is not open, yet the internal structures of the skull, such as the sella turcica, foramina for nerves and vessels, and sulci for venous sinuses were made in considerable accuracy. Moreover, the proper connection of most foramina was proved between the inside and outside of the skull. The skull caps of Kagami and Okuda wooden skeletons are open as those used in the modern medical education.
Assuntos
Anatomia/história , Modelos Anatômicos , Esqueleto , Madeira , Feminino , História do Século XVIII , História do Século XIX , Humanos , Japão , MasculinoRESUMO
We studied the anatomy education and the view of anatomy professors on it in medical and dental schools in Japan. In most schools anatomy is taught in the second year. In medical schools, the systematic education separating macroscopic and microscopic anatomy is prevalent. Although the tutorial system has been introduced in 80% of medical schools, its introduction into anatomy education has remained at 30%. The tutorial system is regarded to be more effective by engaged professors than non-engaged. Some kinds of clinical anatomy education have been introduced in half of the medical schools surveyed. In dental schools, on the other hand, macroscopic and microscopic anatomy tend to be taught in combination. One third of the dental schools have introduced clinical anatomy but few schools have a tutorial system. The overwhelming majority of professors are evaluated by students and have regarded the evaluation useful for improving their teaching. They also have thought that the questionnaire and the timing of the evaluation must be considered carefully, and that the evaluation should not be directly used for purposes other than the improvement of education. We have made the proposals for further improvement in anatomy education based upon this study.
Assuntos
Anatomia/educação , Currículo/tendências , Educação em Odontologia/tendências , Educação de Graduação em Medicina/tendências , Faculdades de Odontologia , Faculdades de Medicina , Educação em Odontologia/métodos , Educação em Odontologia/normas , Educação de Graduação em Medicina/métodos , Educação de Graduação em Medicina/normas , Docentes , Humanos , Japão , Inquéritos e Questionários , Ensino/normasRESUMO
A three-dimensional (3D) clinostat is a device for multidirectional G force generation. By controlled rotation of two axes, a 3D clinostat cancels the cumulative gravity vector at the center of the device and produces an environment with an average of 10(-3) G over time. We cultured a human osteoblast cell line in a 3D clinostat and examined the growth properties and differentiation of the cells, including morphology, histological detection of calcification, and mitogen-activated protein kinase (MAPK) cascades. In a normal 1 G condition, alkaline phosphatase (AlPase) activity was detected on day 7 of culture, bone nodules were formed on day 12, and calcium deposits were seen on day 20. In the 3D clinostat, the cells looked larger and bulged. AlPase activity was detected on day 10 of culture. However, neither bone nodules nor calcification was found in the 3D clinostat up to day 21. The expression levels of core-binding factor A1 (a transcription factor for bone formation) and osteocalcin (a bone matrix protein) increased in the control culture but decreased in culture in 3D clinostat. Phosphorylation of p38(MAPK) (p38) was repressed in culture in 3D clinostat, whereas total p38 as well as total and phosphorylated forms of extracellular signal-regulated kinases and stress-activated protein kinase/jun N-terminal kinase were not changed in the 3D clinostat. When a p38 inhibitor, SB 203580, was added to the culture medium in a normal 1 G environment, AlPase activity and formation of bone nodules and calcium deposits were strongly inhibited. On the other hand, they were inhibited only partially by a MAPK kinase inhibitor, U-0126. On the basis of these results, it is concluded that (1) osteoblast differentiation is inhibited in culture in a 3D clinostat and (2) this inhibition is mainly due to the suppression of p38 phosphorylation.
Assuntos
Técnicas de Cultura de Células/instrumentação , Diferenciação Celular/fisiologia , Sistema de Sinalização das MAP Quinases/fisiologia , Proteínas Quinases Ativadas por Mitógeno/metabolismo , Osteoblastos/metabolismo , Ausência de Peso , Calcificação Fisiológica , Técnicas de Cultura de Células/métodos , Divisão Celular/fisiologia , Sobrevivência Celular , Células Cultivadas , Fatores de Ligação ao Core , Inibidores Enzimáticos/metabolismo , Humanos , Imidazóis/metabolismo , Proteínas de Neoplasias/metabolismo , Osteoblastos/citologia , Osteocalcina/metabolismo , Fosforilação , Piridinas/metabolismo , Fatores de Transcrição/metabolismo , Proteínas Quinases p38 Ativadas por MitógenoRESUMO
This study examined whether a hypothermic environment reduces experimentally-induced atrophy of skeletal muscle, as judged by histochemical findings. The hind limbs of hamsters in a hypothermic group were suspended and flexed into plantar positions at the ankle joint, and housed for one week at 8 to 12 degree celsius in a temperature-controlled room, while the normothermic group was housed at 23 to 25 degree celsius. Hypothermia did not significantly alter the average caloric intake, and the animals from the hypothermic group lost a significant amount of body weight when compared with the normothermic group. The hypothermic group retained more muscle wet-weight and myofibers cross-sectional area in the soleus and gastrocnemius muscles compared with the normothermic group. Our results indicate that a hypothermic environment inhibits short-term muscle atrophy. This inhibition may be caused by the increased caloric intake combined with a state similar to hibernation in low-temperature environments.
Assuntos
Transtornos Musculares Atróficos/fisiopatologia , Temperatura , Animais , Cricetinae , Extremidades , Elevação dos Membros Posteriores/fisiologia , Hipotermia Induzida , MesocricetusRESUMO
In order to identify the apoptosis-induced factors and apoptosis pathway in hindlimb unloading muscle atrophy, the reciprocal relationships between caspase-3 activation and factors related to mitochondria, other organelle pathways, oxidative stress and nitric oxide were investigated. Male Wistar rats were divided into four groups, two groups of hindlimb-unloaded rats were maintained under normal (25 degrees C) and low-temperature (10 degrees C) environmental conditions for a 3-week experimental period, plus two corresponding control groups. Active caspase-3-containing myofibers were observed in the hindlimb-unloaded rats in normal and low-temperature environments, but not in the control rats. In these caspase-3-containing fibers, DNA fragmentation, dystrophin breakdown, increased immunolabeling of mu-calpain, decreased cytochrome c, cathepsin-D effusion from the lysosomes and increased lipid peroxidation were observed, while no changes in active caspase-12, eNOS or nNOS immunolabeling were seen. Furthermore, although caspase-3 activation was observed in type-I fibers, caspase-12 labeling was observed in fibers of the hybrid type. These results show that the apoptosis observed in hindlimb unloading-induced muscle atrophy is caused by activation of the caspase cascade via the lysosome pathway. Moreover, the results suggest that caspase-12 does not activate caspase-3 due to differences in the cell differentiation or the apoptosis-inducing stimulation.
Assuntos
Fator de Indução de Apoptose/fisiologia , Membro Posterior/patologia , Músculo Esquelético/patologia , Transtornos Musculares Atróficos/patologia , Animais , Caspase 3/metabolismo , Temperatura Baixa , Fragmentação do DNA , Imuno-Histoquímica , Masculino , Fibras Musculares Esqueléticas/enzimologia , Fibras Musculares Esqueléticas/patologia , Transtornos Musculares Atróficos/etiologia , Ratos , Ratos Wistar , Suporte de CargaRESUMO
A mouse monoclonal antibody (G9, Horio et al. in Cell Motil Cytoskel 44:284-295, 1999) that was raised against the gamma-tubulin from a fission yeast, Schizosaccharomyces pombe, showed a unique staining in the mouse small intestine. Similar to another anti-gamma-tubulin antibody that is commercially available, G9 showed typical dot-like staining corresponding to the microtubule-organizing center in the free cells of the epithelium and the connective tissue under it. In addition, G9 stained the cell-cell contacts in the epithelium. This stained region was not bicellular but tricellular junctions of the enterocytes. This staining was unique to G9 and was diminished on the sample of the mouse small intestine, which had lost most of its filamentous microtubules through the preparation process. The tricellular junction is thought to be the weakest point of the epithelial barrier, and no other junctional structures have been identified except for the central sealing elements extending from the tight junctions between the two cells. Our results suggest the existence of a new molecule underlying the tricellular junctions, which may relate to gamma-tubulin and the microtubules.
Assuntos
Duodeno/metabolismo , Tubulina (Proteína)/metabolismo , Animais , Anticorpos Monoclonais/imunologia , Bovinos , Células Cultivadas , Cães , Duodeno/ultraestrutura , Epitélio/metabolismo , Epitélio/ultraestrutura , Imunofluorescência , Imuno-Histoquímica , CamundongosRESUMO
AIM: It is hoped that nanoparticles will become ever more useful in the development of nanomedicine. To evaluate the behavior of nanoparticles in solution, we aimed to establish a single optical fiber-illumination method that is easy to integrate with a conventional microscope at low cost. METHODS: Solutions of gold nanoparticles and carbon nanotubes were analyzed in a single optical fiber-illuminated video microscope and the tracks of Brownian motion of these nanoparticles were traced using video images. Their diffusion coefficient was measured by the mean square displacement of the movement. Using the diffusion coefficient in the Stokes-Einstein equation, the hydrodynamic diameter of the nanoparticles in solution was evaluated. RESULTS: The visualization of gold nanoparticles clearly in a high signal-to-noise ratio was achieved. The evaluated particle sizes of gold nanoparticles were similar to those obtained by a transmission electron microscope and the aggregation process of the carbon nanotubes following incubation was also observed and similar size estimation of the aggregates was performed. CONCLUSION: The single fiber-illumination method was applicable to visualize nanoparticle movement clearly and to estimate their sizes in solution. This simple method is suitable for the in situ observation of the nanoparticle-binding process to target cells.
Assuntos
Coloides/química , Tecnologia de Fibra Óptica/instrumentação , Interpretação de Imagem Assistida por Computador/métodos , Microscopia de Vídeo/instrumentação , Microscopia de Vídeo/métodos , Nanopartículas/ultraestrutura , Reconhecimento Automatizado de Padrão/métodos , Algoritmos , Inteligência Artificial , Coloides/análise , Desenho de Equipamento , Análise de Falha de Equipamento , Interpretação de Imagem Assistida por Computador/instrumentação , Fibras Ópticas , Tamanho da Partícula , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Processamento de Sinais Assistido por Computador/instrumentação , SoluçõesRESUMO
Edwardsiella tarda is a pathogen with a broad host range that infects both animals and humans. Resistance to phagocytic killing may be involved in the pathogenicity of this bacterium. Here we show that intracellular replication of E. tarda in murine macrophages is dependent on the type III secretion system and induces an anti-apoptotic effect by up-regulating anti-apoptotic NF-kappaB target genes. The wild-type strain replicates within the phagosomal membrane of macrophages; whereas the type III mutant does not. Microarray analysis shows the mRNA expression level of NF-kappaB target genes (e.g. pro-inflammatory cytokines and anti-apoptotic genes) in macrophages infected with the wild-type strain were up-regulated compared to macrophages infected with the type III mutant. Up-regulation of Bcl2a1a, Bcl2a1b, cIAP-2, and TRAF1 genes induced expression of anti-apoptotic proteins to protect macrophages from apoptosis induced by staurosporine. Further, this protection was inhibited by adding kamebakaurin, an inhibitor of NF-kappaB activation and was confirmed using an NF-kappaB reporter gene assay. Up-regulation of anti-apoptotic NF-kappaB target genes is responsible for the anti-apoptotic activity of E. tarda and is required for intracellular replication in murine macrophages.
Assuntos
Apoptose/fisiologia , Edwardsiella tarda/crescimento & desenvolvimento , Infecções por Enterobacteriaceae/microbiologia , Macrófagos/microbiologia , NF-kappa B/genética , Estaurosporina/farmacologia , Animais , Apoptose/efeitos dos fármacos , Apoptose/genética , Western Blotting , Linhagem Celular , DNA Bacteriano/genética , Diterpenos/farmacologia , Edwardsiella tarda/genética , Edwardsiella tarda/metabolismo , Edwardsiella tarda/patogenicidade , Regulação Bacteriana da Expressão Gênica , Macrófagos/citologia , Macrófagos/efeitos dos fármacos , Macrófagos/fisiologia , Camundongos , Microscopia Eletrônica de Transmissão , Antígenos de Histocompatibilidade Menor , Mutagênese Insercional , NF-kappa B/antagonistas & inibidores , NF-kappa B/biossíntese , Análise de Sequência com Séries de Oligonucleotídeos , Proteínas Proto-Oncogênicas c-bcl-2/biossíntese , Proteínas Proto-Oncogênicas c-bcl-2/genética , Regulação para CimaRESUMO
OBJECTIVE: In the present study, the effect of electrical stimulation was examined for the ability to induce morphological, physiological, and molecular biological effects on myoblasts during cell differentiation. METHODS: L6 rat myoblasts were electrically stimulated by newly developed methods on culture days 6, 8, 10 and 12. RESULTS: This electrical stimulation accelerated the appearance of myotubes, and subsequently produced spontaneously contracting muscle fibers. Measurement of membrane potential showed that the contracting cell had functional ion channels and gap junctional intercellular communication. In the electrically stimulated cells, an enhanced expression of MyoD family and M-cadherin was also observed. Expression of connexin 43 was increased and maintained at a high level in the electrically stimulated cells. CONCLUSION: This is the first demonstration of in vitro induction of myoblasts in spontaneously contractile muscle fibers by intermittent stimulation. This novel method for induction of myoblast differentiation represents an important advance in cell therapy.
Assuntos
Diferenciação Celular , Contração Muscular , Mioblastos Esqueléticos/citologia , Animais , Western Blotting , Caderinas/metabolismo , Comunicação Celular , Linhagem Celular , Forma Celular , Conexina 43/metabolismo , Estimulação Elétrica , Junções Comunicantes/metabolismo , Junções Comunicantes/fisiologia , Imuno-Histoquímica , Potenciais da Membrana , Proteína MyoD/biossíntese , Mioblastos Esqueléticos/metabolismo , Mioblastos Esqueléticos/fisiologia , Fatores de Regulação Miogênica/biossíntese , Ratos , Fatores de Tempo , Regulação para CimaRESUMO
Cytoskeletal microtubules were visualized in the mouse duodenal mucosa by an improved immunofluorescence method using a microtubule-stabilizing reagent, Taxol, and collagenase as an enzymatic epitope retriever. The improvement in immunostaining was shown morphologically and statistically by comparing fluorescence intensities of specimens prepared with or without Taxol and collagenase treatment. In free cells in the epithelium and in the lamina propria, microtubules radiated from the gamma-tubulin-immunostained organizing center. Enterocytes and Brunner's gland cells double-stained with an anti-alpha-tubulin antibody and a lectin (Helix pomatia agglutinin, soybean agglutinin or Ulex europaeus agglutinin-I) showed that microtubules ran along the cell axis and were abundant between the Golgi apparatus and the apical surface. The microtubules appeared to provide a structural support to hold the Golgi apparatus in position and to act as railways for secretory granules, which are transported towards the apical surface. In addition, gamma-tubulin-like immunoreactivity was associated with the Golgi apparatus in the enterocytes. These results show that the method using Taxol and collagenase is effective for visualizing microtubules in epithelial cells, and that microtubules may play important roles in both positioning of the Golgi apparatus and transport of secretory granules. Our results also support the idea that the Golgi apparatus may act as an organizing center for microtubules.
Assuntos
Colagenases , Enterócitos/ultraestrutura , Complexo de Golgi/ultraestrutura , Microtúbulos/ultraestrutura , Paclitaxel , Coloração e Rotulagem/métodos , Animais , Glândulas Duodenais/citologia , Imunofluorescência/métodos , Masculino , Camundongos , Camundongos Endogâmicos ICR , Microscopia ConfocalRESUMO
Parietal cells in the rat oxyntic mucosa were analyzed by the immunofluorescence pattern of the proton pump. The adult rats were grouped into fasting (C), gastrin-treated (G), and ranitidine-treated (R) groups, gastric pH was measured, and the stomach was processed for immunohistochemistry. The fluorescence of parietal cells showed a reticular, diffuse, or mixed pattern in cytoplasm. Quantitatively, 53% of the total cells showed the reticular pattern in group G (pH 1.9), 44% in group C (pH 2.0), and 0% in group R (pH 6.7). On the other hand, 7.0% of the total cells showed the diffuse pattern in group G, 11.9% in group C, and 56.2% in group R. The results indicated that the staining pattern depended on the activity of acid secretion. In addition, the proportion of parietal cells showing the reticular pattern decreased in the following order, the superficial, middle, and deep third of the mucosa, and the diffuse pattern showed the opposite trend. This suggests that the acid secretion is more active in parietal cells in the superficial part of the mucosa. The double staining with proton pump-specific and cytochrome oxidase-specific antibodies revealed the close relation between reticular fluorescence and mitochondria.
Assuntos
Ácido Gástrico/metabolismo , ATPase Trocadora de Hidrogênio-Potássio/metabolismo , Estômago/enzimologia , Animais , Contagem de Células , Imunofluorescência , Mucosa Gástrica/metabolismo , Concentração de Íons de Hidrogênio , Masculino , Microscopia Confocal , Mitocôndrias/enzimologia , Células Parietais Gástricas/citologia , Ratos , Ratos WistarRESUMO
During continuous ambulatory peritoneal dialysis, the peritoneum is directly and continuously exposed to unphysiologic peritoneal dialysis fluid; the resulting mesothelial damage has been suggested to cause loss of ultrafiltration and dialysis efficacy. The present study investigated the effect of a high glucose concentration on cultured human peritoneal mesothelial cells to clarify the cause of decreased dialysis efficacy during prolonged peritoneal dialysis. High glucose caused a concentration-dependent decrease in cell proliferation, damage to the intercellular junctions, and excess production of transforming growth factor-beta (TGF-beta). The levels of intercellular junctional proteins (ZO-1, E-cadherin, and beta-catenin) were decreased, and immuno-staining by anti-ZO-1 and anti- beta-catenin antibodies became weaker and often discontinuous along the cell contour. Mannitol had similar but weaker effects at the same osmolality, and an anti-TGF-beta neutralizing antibody reduced the effects of high glucose. Therefore, these effects were induced not only by glucose itself but also by hyperosmolality and by a glucose-induced increase of TGF-beta. These findings suggest that the peritoneal mesothelium is damaged by prolonged peritoneal dialysis using high glucose dialysate and that impairment of the intercellular junctions of peritoneal mesothelial cells by high glucose dialysate induces peritoneal hyperpermeability and a progressive reduction in dialysis efficacy.
Assuntos
Glucose/farmacologia , Junções Intercelulares/efeitos dos fármacos , Peritônio/efeitos dos fármacos , Anticorpos/farmacologia , Western Blotting , Divisão Celular/efeitos dos fármacos , Células Cultivadas , Colorimetria , Células Epiteliais/efeitos dos fármacos , Humanos , Técnicas Imunológicas , Peritônio/citologia , Proteínas Recombinantes/farmacologia , Coloração e Rotulagem , Fator de Crescimento Transformador beta/biossíntese , Fator de Crescimento Transformador beta/imunologia , Fator de Crescimento Transformador beta/farmacologia , Fator de Crescimento Transformador beta1RESUMO
CD13, a receptor for human coronavirus 229E (HCoV-229E), was identified as a major component of the Triton X-100-resistant membrane microdomain in human fibroblasts. The incubation of living fibroblasts with an anti-CD13 antibody on ice gave punctate labeling that was evenly distributed on the cell surface, but raising the temperature to 37 degrees C before fixation caused aggregation of the labeling. The aggregated labeling of CD13 colocalized with caveolin-1 in most cells. The HCoV-229E virus particle showed a binding and redistribution pattern that was similar to that caused by the anti-CD13 antibody: the virus bound to the cell evenly when incubated on ice but became colocalized with caveolin-1 at 37 degrees C; importantly, the virus also caused sequestration of CD13 to the caveolin-1-positive area. Electron microscopy confirmed that HCoV-229E was localized near or at the orifice of caveolae after incubation at 37 degrees C. The depletion of plasmalemmal cholesterol with methyl beta-cyclodextrin significantly reduced the HCoV-229E redistribution and subsequent infection. A caveolin-1 knockdown by RNA interference also reduced the HCoV-229E infection considerably. The results indicate that HCoV-229E first binds to CD13 in the Triton X-100-resistant microdomain, then clusters CD13 by cross-linking, and thereby reaches the caveolar region before entering cells.
Assuntos
Antígenos CD13/metabolismo , Cavéolas/virologia , Coronavirus Humano 229E/patogenicidade , Microdomínios da Membrana/metabolismo , Sequência de Aminoácidos , Animais , Caveolina 1 , Caveolinas/genética , Caveolinas/metabolismo , Células Cultivadas , Coronavirus Humano 229E/metabolismo , Fibroblastos/virologia , Humanos , Microscopia Eletrônica , Microscopia de Fluorescência , Dados de Sequência Molecular , Interferência de RNA , RNA Interferente Pequeno , Receptores Virais/metabolismo , Pele/citologiaRESUMO
Phosphorylation of connexin 43 (Cx43) molecules (e.g. by extracellular signal-regulated kinase) leads to reductions in gap-junctional intercellular communication (GJIC). GJIC levels also appear to be lower in the presence of p38 mitogen-activated protein (MAP) kinase, for unknown reasons. In this study, we used assays of the recovery of fluorescence by photobleached WB-F344 cells to demonstrate that GJIC levels are decreased by anisomycin [a protein synthesis inhibitor as well as an activator of p38 MAP kinase and c-Jun N-terminal kinases (JNK)] as a result of time-dependent depletion of the phosphorylated forms of Cx43. Using immunohistochemistry, we also detected far less of the Cx43 proteins at cell borders. These findings agree with the photobleaching assay results. Moreover, prior treatment with SB203580 (a specific inhibitor of p38 MAP kinase) appeared to be effective in preventing the loss of phosphorylated forms of Cx43 and the loss of Cx43 proteins at cell borders. Total protein labelling with [(35)S]-methionine and [(32)P]-orthophosphates labelling of Cx43 showed that anisomycin enhanced the phosphorylation level of Cx43 along with inhibition of protein synthesis. SB203580 prevented the former but not the latter. The effect of anisomycin on GJIC was not dependent on the inhibition of protein synthesis because the addition of SB203580 completely maintained the level of GJIC without restoring protein synthesis. The Cx43 phosphorylation level increased by anisomycin treatment, whereas the amount of phosphorylated forms of Cx43 decreased, suggesting that activation of Cx43 phosphorylation might lead to the loss of Cx43. These results suggest that activation of p38 MAP kinase leads to reduction in the levels of phosphorylated forms of Cx43, possibly owing to accelerated degradation, and that these losses might be responsible for the reduction in numbers of gap junctions and in GJIC.