Ptychographic lensless coherent endomicroscopy through a flexible fiber bundle.

Conventional fiber-bundle-based endoscopes allow minimally invasive imaging through flexible multi-core fiber (MCF) bundles by placing a miniature lens at the distal tip and using each core as an imaging pixel. In recent years, lensless imaging through MCFs was made possible by correcting the core-to-core phase distortions pre-measured in a calibration procedure. However, temporally varying wavefront distortions, for instance, due to dynamic fiber bending, pose a challenge for such approaches. Here, we demonstrate a coherent lensless imaging technique based on intensity-only measurements insensitive to core-to-core phase distortions. We leverage a ptychographic reconstruction algorithm to retrieve the phase and amplitude profiles of reflective objects placed at a distance from the fiber tip, using as input a set of diffracted intensity patterns reflected from the object when the illumination is scanned over the MCF cores. Our approach thus utilizes an acquisition process equivalent to confocal microendoscopy, only replacing the single detector with a camera.

Image scanning lensless fiber-bundle endomicroscopy.

Fiber-based confocal endomicroscopy has shown great promise for minimally-invasive deep-tissue imaging. Despite its advantages, confocal fiber-bundle endoscopy inherently suffers from undersampling due to the spacing between fiber cores, and low collection efficiency when the target is not in proximity to the distal fiber facet. Here, we demonstrate an adaptation of image-scanning microscopy (ISM) to lensless fiber bundle endoscopy, doubling the spatial sampling frequency and significantly improving collection efficiency. Our approach only requires replacing the confocal detector with a camera. It improves the spatial resolution for targets placed at a distance from the fiber tip, and addresses the fundamental challenge of aliasing/pixelization artifacts.

Three-dimensional broadband light beam manipulation in forward scattering samples.

Focusing light into highly disordered biological tissue is a major challenge in optical microscopy and biomedical imaging due to scattering. However, correlations in the scattering matrix, known as "memory effects", can be used to improve imaging capabilities. Here we discuss theoretically and numerically the possibility to achieve three-dimensional ultrashort laser focusing and scanning inside forward scattering media, beyond the scattering mean free path, by simultaneously taking advantage of the angular and the chromato-axial memory effects. The numerical model is presented in details, is validated within the state of the art theoretical and experimental framework and is finally used to propose a scheme for focusing ultra-short laser pulses in depth through forward scattering media.

Everything Seems So Illogical: Constructing Missingness Between Life and Death in Israel.

This paper discusses the case of missing persons in Israel, to show how the category of "missingness" is constructed by the people who have been left behind, and how this may threaten the life-death dichotomy assumption. The field of missing persons in Israel is characterized not only by high uncertainty, but also by the absence of relevant cultural scripts. Based on a narrative ethnography of missingness in Israel, I claim that a new and subversive social category of "missingness" can be constructed following the absence of cultural scripts. The left-behinds fluctuate not only between different assumptions about the missing person's fate; they also fluctuate between acceptance of the life-death dichotomy, thus yearning for a solution to a temporary in-between state, and blurring this dichotomy, and thus constructing "missingness" as a new stable and subversive ontological category. Under this category, new rites of passage are also negotiated and constructed.

100,000 frames-per-second compressive imaging with a conventional rolling-shutter camera by random point-spread-function engineering.

We demonstrate an approach that allows taking videos at very high frame-rates of over 100,000 frames per second by exploiting the fast sampling rate of the standard rolling-shutter readout mechanism, common to most conventional sensors, and a compressive-sampling acquisition scheme. Our approach is directly applied to a conventional imaging system by the simple addition of a diffuser to the pupil plane that randomly encodes the entire field-of-view to each camera row, while maintaining diffraction-limited resolution. A short video is reconstructed from a single camera frame via a compressed-sensing reconstruction algorithm, exploiting the inherent sparsity of the imaged scene.

Using fiber-bending-generated speckles for improved working distance and background rejection in lensless micro-endoscopy.

Lensless flexible fiber-bundle-based endoscopes allow imaging at depths beyond the reach of conventional microscopes with a minimal footprint. These multicore fibers provide a simple solution for wide-field fluorescent imaging when the target is adjacent to the fiber facet. However, they suffer from a very limited working distance and out-of-focus background. Here, we carefully study the dynamic speckle illumination patterns generated by bending a commercial fiber bundle and show that they can be exploited to allow extended working distance and background rejection, using a super-resolution fluctuations imaging analysis of multiple frames, without the addition of any optical elements.

Noninvasive focusing through scattering layers using speckle correlations.

Angular speckle correlations known as the "memory effect" have recently been exploited for noninvasive imaging through scattering layers. Here we show that the information obtained from speckle correlations can be used as a noninvasive feedback mechanism for wavefront shaping. We utilize this feedback to demonstrate noninvasive diffraction-limited focusing of coherent light through thin scattering layers.

Self-Driven Fractional Rotational Diffusion of the Harmonic Three-Mass System.

In flat space, changing a system's velocity requires the presence of an external force. However, an isolated nonrigid system can freely change its orientation due to the nonholonomic nature of the angular momentum conservation law. Such nonrigid isolated systems may thus manifest their internal dynamics as rotations. In this work, we show that for such systems chaotic internal dynamics may lead to macroscopic rotational random walk resembling thermally induced motion. We do so by studying the classical harmonic three-mass system in the strongly nonlinear regime, the simplest physical model capable of zero angular momentum rotation as well as chaotic dynamics. At low energies, the dynamics are regular and the system rotates at a constant rate with zero angular momentum. For sufficiently high energies a rotational random walk is observed. For intermediate energies the system performs ballistic bouts of constant rotation rates interrupted by unpredictable orientation reversal events, and the system constitutes a simple physical model for Lévy walks. The orientation reversal statistics in this regime lead to a fractional rotational diffusion that interpolates smoothly between the ballistic and regular diffusive regimes.

(Un) Natural Grief: Novelty, Tradition and Naturalization in Israeli Discourse on Posthumous Reproduction.

This article examines Israeli discourse on posthumous reproduction (PR) and the related cultural construction of "(un)natural" grief. Based mainly on an analysis of in-depth interviews with family members who submitted a request for PR, we examine the regimes of justification used by supporters and opponents of this technology. With both sides using the notion of "nature" to support their claim, the dispute centers on whether PR constructs a new social expression of grief (and hence should be seen as unnatural) or is only a reflection of an age-old grieving process (and is thus natural). We argue that by employing a twofold, novel/traditional justification, PR supporters aim to go one step further, from a symbolic continuity of the dead to a so-called real one. This progression highlights the flexibility of the natural category at the intersection of technology and culture and the abandonment of such binary distinctions as life/death and nature/culture.

Two-photon lensless micro-endoscopy with in-situ wavefront correction.

Multi-core fiber-bundle endoscopes provide a minimally-invasive solution for deep tissue imaging and opto-genetic stimulation, at depths beyond the reach of conventional microscopes. Recently, wavefront-shaping has enabled lensless bundle-based micro-endoscopy by correcting the wavefront distortions induced by core-to-core inhomogeneities. However, current wavefront-shaping solutions require access to the fiber distal end for determining the bend-sensitive wavefront-correction. Here, we show that it is possible to determine the wavefront correction in-situ, without any distal access. Exploiting the nonlinearity of two-photon excited fluorescence, we adaptively determine the wavefront correction in-situ using only proximal detection of epi-detected fluorescence. We experimentally demonstrate diffraction-limited, three-dimensional, two-photon lensless microendoscopy with commercially-available ordered- and disordered multi-core fiber bundles.

Depth-resolved speckle-correlations imaging through scattering layers via coherence gating.

Recently, novel imaging techniques based on the "memory-effect" speckle-correlations have enabled diffraction-limited imaging through scattering layers and around corners. These techniques, however, are currently limited to imaging only small planar objects contained within the angular and axial range of the memory effect. In addition, they do not provide depth information or depth-sectioning capability. Here, we extend speckle-correlation imaging to include high-resolution depth-sectioning capability in the reflection mode, by combining it with coherence gating via low-coherence holography. We demonstrate depth measurements of reflective targets through a scattering layer, and speckle-correlation imaging using coherence-gated scattered light.

Photoacoustic imaging beyond the acoustic diffraction-limit with dynamic speckle illumination and sparse joint support recovery.

In deep tissue photoacoustic imaging the spatial resolution is inherently limited by the acoustic wavelength. Recently, it was demonstrated that it is possible to surpass the acoustic diffraction limit by analyzing fluctuations in a set of photoacoustic images obtained under unknown speckle illumination patterns. Here, we purpose an approach to boost reconstruction fidelity and resolution, while reducing the number of acquired images by utilizing a compressed sensing computational reconstruction framework. The approach takes into account prior knowledge of the system response and sparsity of the target structure. We provide proof of principle experiments of the approach and demonstrate that improved performance is obtained when both speckle fluctuations and object priors are used. We numerically study the expected performance as a function of the measurement's signal to noise ratio and sample spatial-sparsity. The presented reconstruction framework can be applied to analyze existing photoacoustic experimental data sets containing dynamic fluctuations.

Widefield lensless imaging through a fiber bundle via speckle correlations.

Flexible fiber-optic endoscopes provide a solution for imaging at depths beyond the reach of conventional microscopes. Current endoscopes require focusing and/or scanning mechanisms at the distal end, which limit miniaturization, frame-rate, and field of view. Alternative wavefront-shaping based lensless solutions are extremely sensitive to fiber-bending. We present a lensless, bend-insensitive, single-shot imaging approach based on speckle-correlations in fiber bundles that does not require wavefront shaping. Our approach computationally retrieves the target image by analyzing a single camera frame, exploiting phase information that is inherently preserved in propagation through convnetional fiber bundles. Unlike conventional fiber-based imaging, planar objects can be imaged at variable working distances, the resulting image is unpixelated and diffraction-limited, and miniaturization is limited only by the fiber diameter.

Single-shot diffraction-limited imaging through scattering layers via bispectrum analysis.

Recently introduced speckle correlations-based techniques enable noninvasive imaging of objects hidden behind scattering layers. In these techniques, the hidden object Fourier amplitude is retrieved from the scattered light autocorrelation, and the lost Fourier phase is recovered via iterative phase-retrieval algorithms, which suffer from convergence to wrong local minimums solutions and cannot solve ambiguities in object orientation. Here, inspired by notions used in astronomy, we experimentally demonstrate that in addition to Fourier amplitude, the object-phase information is naturally and inherently encoded in the scattered light bispectrum (the Fourier transform of triple correlation) and can also be extracted from a single high-resolution speckle pattern, based on which we present a single-shot imaging scheme to deterministically and unambiguously retrieve diffraction-limited images of objects hidden behind scattering layers.

Widefield lensless endoscopy with a multicore fiber.

We demonstrate pixelation-free real-time widefield endoscopic imaging through an aperiodic multicore fiber (MCF) without any distal opto-mechanical elements or proximal scanners. Exploiting the memory effect in MCFs, the images in our system are directly obtained without any post-processing using a static wavefront correction obtained from a single calibration procedure. Our approach allows for video-rate 3D widefield imaging of incoherently illuminated objects with imaging speed not limited by the wavefront-shaping device refresh rate.

Spatiotemporal Coherent Control of Light through a Multiple Scattering Medium with the Multispectral Transmission Matrix.

We report the broadband characterization of the propagation of light through a multiple scattering medium by means of its multispectral transmission matrix. Using a single spatial light modulator, our approach enables the full control of both the spatial and spectral properties of an ultrashort pulse transmitted through the medium. We demonstrate spatiotemporal focusing of the pulse at any arbitrary position and time with any desired spectral shape. Our approach opens new perspectives for fundamental studies of light-matter interaction in disordered media, and has potential applications in sensing, coherent control, and imaging.

Reference-less measurement of the transmission matrix of a highly scattering material using a DMD and phase retrieval techniques.

This paper investigates experimental means of measuring the transmission matrix (TM) of a highly scattering medium, with the simplest optical setup. Spatial light modulation is performed by a digital micromirror device (DMD), allowing high rates and high pixel counts but only binary amplitude modulation. On the sensor side, without a reference beam, the CCD camera provides only intensity measurements. Within this framework, this paper shows that the TM can still be retrieved, through signal processing techniques of phase retrieval. This is experimentally validated on three criteria : quality of prediction, distribution of singular values, and quality of focusing.

Single-pulse CARS based multimodal nonlinear optical microscope for bioimaging.

Noninvasive label-free imaging of biological systems raises demand not only for high-speed three-dimensional prescreening of morphology over a wide-field of view but also it seeks to extract the microscopic functional and molecular details within. Capitalizing on the unique advantages brought out by different nonlinear optical effects, a multimodal nonlinear optical microscope can be a powerful tool for bioimaging. Bringing together the intensity-dependent contrast mechanisms via second harmonic generation, third harmonic generation and four-wave mixing for structural-sensitive imaging, and single-beam/single-pulse coherent anti-Stokes Raman scattering technique for chemical sensitive imaging in the finger-print region, we have developed a simple and nearly alignment-free multimodal nonlinear optical microscope that is based on a single wide-band Ti:Sapphire femtosecond pulse laser source. Successful imaging tests have been realized on two exemplary biological samples, a canine femur bone and collagen fibrils harvested from a rat tail. Since the ultra-broad band-width femtosecond laser is a suitable source for performing high-resolution optical coherence tomography, a wide-field optical coherence tomography arm can be easily incorporated into the presented multimodal microscope making it a versatile optical imaging tool for noninvasive label-free bioimaging.

Scanning-free imaging through a single fiber by random spatio-spectral encoding.

We present an approach for 2D imaging through a single fiber without the need for scanners. A random scattering medium placed next to the distal end of the fiber is used to encode the collected light from every imaged pixel with a different random spectral signature. We demonstrate imaging of externally illuminated 2D objects from a single measured spectrum at the fiber's proximal end. The technique is insensitive to fiber bending, an advantage for endoscopic applications.

Improving photoacoustic-guided optical focusing in scattering media by spectrally filtered detection.

We study the potential of photoacoustic guidance for light focusing through scattering samples via wavefront-shaping and iterative optimization. We experimentally demonstrate that the focusing efficiency on an extended absorber can be improved by iterative optimization of the high frequency components of the broadband photoacoustic signal detected with a spherically focused transducer. We demonstrate more than 12-fold increase in the photoacoustic signal generated by a 30 µm wire using a narrow frequency band around 60 MHz. By monitoring the speckle pattern evolution during the optimization process with a CCD camera, we experimentally confirm that such optimization leads to a smaller optical focus than what would be obtained by optimizing lower frequencies of the photoacoustic feedback.