RESUMO
In nonobese diabetic (NOD) mice, beta-cell reactive T-helper type 1 (Th1) responses develop spontaneously and gradually spread, creating a cascade of responses that ultimately destroys the beta-cells. The diversity of the autoreactive T-cell repertoire creates a major obstacle to the development of therapeutics. We show that even in the presence of established Th1 responses, it is possible to induce autoantigen-specific anti-inflammatory Th2 responses. Immune deviation of T-cell responses to the beta-cell autoantigen glutamate decarboxylase (GAD65), induced an active form of self-tolerance that was associated with an inhibition of disease progression in prediabetic mice and prolonged survival of syngeneic islet grafts in diabetic NOD mice. Thus, modulation of autoantigen-specific Th1/Th2 balances may provide a minimally invasive means of downregulating established pathogenic autoimmune responses.
Assuntos
Autoantígenos/uso terapêutico , Diabetes Mellitus Tipo 1/prevenção & controle , Glutamato Descarboxilase/uso terapêutico , Sobrevivência de Enxerto/efeitos dos fármacos , Transplante das Ilhotas Pancreáticas , Células Th2/imunologia , Transferência Adotiva , Animais , Autoanticorpos/sangue , Diabetes Mellitus Tipo 1/imunologia , Diabetes Mellitus Tipo 1/cirurgia , Progressão da Doença , Feminino , Interferon gama/metabolismo , Interleucina-4/metabolismo , Interleucina-5/metabolismo , Ativação Linfocitária , Camundongos , Camundongos Endogâmicos NOD , Pancreatina/imunologia , Tolerância a Antígenos Próprios , Baço/imunologia , Células Th1/imunologiaRESUMO
Limited regions of amino acid sequence similarity frequently occur between microbial antigens and host proteins. It has been widely anticipated that during infection such sequence similarities could induce cross-reactive T cell responses, thereby initiating T cell-mediated autoimmune disease. However, the nature of major histocompatibility complex (MHC)-restricted antigen presentation confers a number of constraints that should make this type of T cell cross-reactivity a rare, MHC allele-dependent event. We tested this prediction using two insulin-dependent diabetes mellitus (IDDM)-associated antigens, coxsackievirus P2-C (Cox P2-C) protein and glutamate decarboxylase (GAD65), which share a prototypic sequence similarity of six consecutive amino acids within otherwise unrelated proteins. We surveyed a panel of 10 murine MHC class II alleles that encompass the spectrum of standard alleles for the ability to cross-reactively present Cox P2-C and GAD65. Out of the 10 restriction elements tested, the sequence similarity regions were both dominant determinants and were cross-reactively displayed after the natural processing of whole antigens, only in the context of I-Anod. These data show that cross-reactive T cell recognition of sequence similarity regions in unrelated proteins is confined to certain MHC alleles, which may explain MHC association with autoimmune disease. It is striking that these two diabetes-associated antigens were cross-reactively recognized only in the context of a diabetes susceptibility allele. Since the human and the murine class II alleles associated with IDDM share conserved features, cross-reactive T cell recognition of GAD65 and Cox P2-C may contribute to the pathogenesis of human IDDM and account for the epidemiological association of coxsackievirus with IDDM.
Assuntos
Alelos , Diabetes Mellitus Tipo 1/genética , Enterovirus/imunologia , Glutamato Descarboxilase/imunologia , Linfócitos T/imunologia , Proteínas Virais/imunologia , Sequência de Aminoácidos , Animais , Reações Cruzadas , Diabetes Mellitus Tipo 1/etiologia , Haplótipos , Antígenos de Histocompatibilidade Classe II/genética , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos NOD , Dados de Sequência MolecularRESUMO
The nature (Th1 versus Th2) and dynamics of the autoimmune response during the development of insulin-dependent diabetes mellitus (IDDM) and after immunotherapy are unclear. Here, we show in nonobese diabetic (NOD) mice that the autoreactive T cell response starts and spreads as a pure Th1 type autoimmunity, suggesting that a spontaneous Th1 cascade underlies disease progression. Surprisingly, induction of antiinflammatory Th2 responses to a single beta cell antigen (betaCA) resulted in the spreading of Th2 cellular and humoral immunity to unrelated betaCAs in an infectious manner and protection from IDDM. The data suggest that both Th1 and Th2 autoimmunity evolve in amplificatory cascades by generating site-specific, but not antigen-specific, positive feedback circuits. Determinant spreading of Th2 responses may be a fundamental mechanism underlying antigen-based immunotherapeutics, explaining observations of infectious tolerance and providing a new theoretical framework for therapeutic intervention.
Assuntos
Autoantígenos/imunologia , Autoimunidade , Ilhotas Pancreáticas/imunologia , Células Th2/imunologia , Animais , Autoimunidade/efeitos dos fármacos , Diabetes Mellitus Tipo 1/imunologia , Feminino , Glutamato Descarboxilase/imunologia , Interferon gama/farmacologia , Interleucina-4/farmacologia , Interleucina-5/farmacologia , Camundongos , Camundongos Endogâmicos AKR , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos NOD , Proteína Básica da Mielina/imunologia , Linfócitos T/química , Linfócitos T/imunologia , Células Th2/efeitos dos fármacosRESUMO
We previously demonstrated that a spontaneous Th1 response against glutamate decarboxylase (GAD65) arises in NOD mice at four weeks in age and subsequently T cell autoimmunity spreads both intramolecularly and intermolecularly. Induction of passive tolerance to GAD65, through inactivation of reactive T cells before the onset of autoimmunity, prevented determinant spreading and the development of insulin-dependent diabetes mellitus (IDDM). Here, we examined whether an alternative strategy, designed to induce active tolerance via the engagement of Th2 immune responses to GAD65, before the spontaneous onset of autoimmunity, could inhibit the cascade of Th1 responses that lead to IDDM. We observed that a single intranasal administration of GAD65 peptides to 2-3-wk-old NOD mice induced high levels of IgG1 antibodies to GAD65. GAD65 peptide treated mice displayed greatly reduced IFN gamma responses and increased IL-5 responses to GAD65, confirming the diversion of the spontaneous GAD65 Th1 response toward a Th2 phenotype. Consistent with the induction of an active tolerance mechanism, splenic CD4+ (but not CD8+) T cells from GAD65 peptide-treated mice, inhibited the adoptive transfer of IDDM to NOD-scid/scid mice. This active mechanism not only inhibited the development of proliferative T cell responses to GAD65, it also limited the expansion of autoreactive T cell responses to other beta cell antigens (i.e., determinant spreading). Finally, GAD65 peptide treatment reduced insulitis and long-term IDDM incidence. Collectively, these data suggest that the nasal administration of GAD65 peptides induces a Th2 cell response that inhibits the spontaneous development of autoreactive Th1 responses and the progression of beta cell autoimmunity in NOD mice.
Assuntos
Diabetes Mellitus Tipo 1/prevenção & controle , Glutamato Descarboxilase/uso terapêutico , Fragmentos de Peptídeos/uso terapêutico , Células Th2/imunologia , Administração Intranasal , Animais , Autoanticorpos/biossíntese , Diabetes Mellitus Tipo 1/imunologia , Feminino , Glutamato Descarboxilase/imunologia , Tolerância Imunológica , Imunoglobulina G/biossíntese , Isotipos de Imunoglobulinas/biossíntese , Imunoterapia Adotiva , Incidência , Interferon gama/biossíntese , Interleucina-5/biossíntese , Ilhotas Pancreáticas/patologia , Camundongos , Camundongos Endogâmicos NOD , Camundongos SCID , Fragmentos de Peptídeos/imunologia , Células Th1/imunologiaRESUMO
Glutamate decarboxylase (GAD; E.C. 4.1.1.15) converts glutamate to gamma-aminobutyric acid (GABA), the major inhibitory neurotransmitter in the vertebrate central nervous system. This report describes the isolation of a GAD complementary DNA clone by immunological screening of a lambda gt-11 brain complementary DNA expression library. The fusion protein produced by this clone catalyzes the conversion of glutamate to GABA and carbon dioxide, confirming its identity as GAD. Antibodies to beta-galactosidase remove GAD enzymatic activity from solution, showing that this activity is associated with the fusion protein. In immunoblotting experiments all three available antisera to GAD reacted with the fusion polypeptide and with two major polypeptides (molecular size, 60,000 and 66,000 daltons) in brain extracts.
Assuntos
Encéfalo/enzimologia , Glutamato Descarboxilase/metabolismo , Ácido gama-Aminobutírico/biossíntese , Animais , Clonagem Molecular , DNA/genética , Escherichia coli/metabolismo , Glutamato Descarboxilase/biossíntese , Glutamato Descarboxilase/genética , Humanos , Camundongos , Ratos , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/metabolismoRESUMO
Insulin-dependent diabetes (IDD) results from the autoimmune destruction of the insulin-producing pancreatic beta cells. Autoreactive T-lymphocytes are thought to play a pivotal role in the pathogenesis of IDD; however, the target antigens of these cells, as well as the inductive events in the disease, are unclear. PBMC in persons with or at increased risk for IDD show elevated reactivity to the beta cell enzyme glutamate decarboxylase (GAD). To identify the T-lymphocyte-reactive determinants of GAD, an overlapping set of synthetic peptides was used to stimulate the PBMC from these individuals, PBMC responsiveness to GAD peptides was not restricted to those with IDD, and a number of peptides elicited responses in PBMC. However, the major determinant of GAD recognized by persons at increased risk for IDD was amino acids 247-279, a region which has significant sequence similarity to the P2-C protein of Coxsackie B virus (47% of 15 increased risk [islet cell autoantibody-positive relatives]; 25% of 16 newly diagnosed IDD patients; and 0% of 13 healthy control subjects). Responses to tetanus and insulin antigens were not different between the study groups. In addition, PBMC from individuals responding to GAD peptides within 247-279 also responded to a Coxsackie viral peptide (i.e., P2-C amino acids 32-47), an observation supporting potential molecular mimicry in this immune response. Although the role of environmental agents in the pathogenesis of the disease remains unclear, these cellular immunological findings support the epidemiological evidence suggesting an inductive role for enteroviruses like Coxsackie B in the autoimmunity underlying IDD.
Assuntos
Diabetes Mellitus Tipo 1/imunologia , Enterovirus/imunologia , Glutamato Descarboxilase/imunologia , Adolescente , Adulto , Sequência de Aminoácidos , Anticorpos Antivirais/sangue , Criança , Diabetes Mellitus Tipo 1/virologia , Feminino , Humanos , Imunidade Celular , Masculino , Pessoa de Meia-Idade , Dados de Sequência Molecular , Fragmentos de Peptídeos/imunologiaRESUMO
Insulin-dependent diabetes mellitus (IDDM) is thought to result from the autoimmune destruction of the insulin-producing beta cells of the pancreas. Years before IDDM symptoms appear, we can detect autoantibodies to one or both forms of glutamate decarboxylase (GAD65 and GAD67), synthesized from their respective cDNAs in a bacterial expression system. Individual IDDM sera show distinctive profiles of epitope recognition, suggesting different humoral immune responses. Although the level of GAD autoantibodies generally decline after IDDM onset, patients with IDDM-associated neuropathies have high levels of antibodies to GAD, years after the appearance of clinical IDDM. We note a striking sequence similarity between the two GADs and Coxsackievirus, a virus that has been associated with IDDM both in humans and in experimental animals. This similarity suggests that molecular mimicry may play a role in the pathogenesis of IDDM.
Assuntos
Autoimunidade/imunologia , Diabetes Mellitus Tipo 2/etiologia , Glutamato Descarboxilase/imunologia , Isoenzimas/imunologia , Sequência de Aminoácidos , Autoanticorpos/sangue , Autoanticorpos/imunologia , Autoantígenos/imunologia , Sistema Nervoso Autônomo/imunologia , Sistema Nervoso Autônomo/patologia , Diabetes Mellitus Tipo 2/complicações , Diabetes Mellitus Tipo 2/imunologia , Neuropatias Diabéticas/etiologia , Neuropatias Diabéticas/imunologia , Enterovirus/genética , Enterovirus/imunologia , Epitopos/imunologia , Glutamato Descarboxilase/genética , Humanos , Ilhotas Pancreáticas/enzimologia , Ilhotas Pancreáticas/imunologia , Dados de Sequência Molecular , Peptídeos/genética , Peptídeos/imunologia , Homologia de Sequência do Ácido Nucleico , Proteínas Virais/genética , Proteínas Virais/imunologiaRESUMO
Individuals with or at risk for insulin-dependent diabetes (IDD) frequently have autoantibodies against an islet cell cytoplasmic (ICA) antigen thought to be a sialoglycolipid. However, we now report that preabsorption of ICA-positive sera with recombinant glutamate decarboxylase (human GAD 65 and/or GAD 67) reduced or blocked the ICA reactivity of 5/18 (27%) new-onset IDD patients and 7/18 (39%) prediabetics. Interestingly, nondiabetic subjects with ICA of > or = 5 yr in duration had GAD-reactive ICA significantly more often (16/24, 67%, P < 0.04) than the diabetic groups. ICA reactivity to GAD was not related to serum ICA titer nor the age of the individual, and in all cases tested was blocked by GAD 65 or GAD 67 with equivalent efficiency. The ICA observed in 21/25 (84%) IDD patients with ICA long after clinical onset of disease (9-42 yr) was reactive to GAD. A natural history analysis of three individuals showed conversions from ICA which was reactive to GAD to a non-GAD-reactive ICA nearer to their clinical onsets of IDD. This study further defines the autoantigens reactive to ICA, and suggests that, whereas ICA that are not reactive to GAD may identify an advanced and more prognostic lesion, GAD-reactive ICA may typify the early or inductive lesion that may or may not progress to clinically significant beta cell injury.
Assuntos
Autoanticorpos/sangue , Diabetes Mellitus Tipo 1/imunologia , Glutamato Descarboxilase/imunologia , Ilhotas Pancreáticas/imunologia , Adolescente , Anticorpos Monoclonais , Clonagem Molecular , Diabetes Mellitus Tipo 1/sangue , Feminino , Glutamato Descarboxilase/genética , Antígenos HLA/análise , Humanos , Masculino , Fases de Leitura Aberta , Proteínas Recombinantes/imunologiaRESUMO
We report the cloning and cDNA sequence of murine GAD65. Murine GAD65 is comprised of 585 amino acids and shares a high degree of homology with human and rat GAD65, with most divergences occurring near their amino-termini. The murine GAD65 sequence will allow evaluation of the role of this gene in murine neurogenetic and autoimmune diseases.
Assuntos
DNA Complementar/química , Glutamato Descarboxilase/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Clonagem Molecular , Camundongos , Dados de Sequência Molecular , Homologia de Sequência de AminoácidosRESUMO
A workshop on autoreactive T-cell responses in NOD mice was held to optimize autoreactive T-cell detection methodologies. Using different proliferation assay protocols, 1 of the 11 participating laboratories detected spontaneous T-cell responses to GAD(524-543) and insulin(9-23) in their NOD mice. Two other laboratories were able to detect autoreactive responses when using enzyme-linked immunospot assay (ELISPOT) and enzyme-linked immunosorbent assay (ELISA) analysis of cytokines in culture supernatants, suggesting that these assays provided greater sensitivity. To address the divergent findings, a follow-up mini-workshop tested NOD mice from four different colonies side-by-side for T-cell proliferative responses to an expanded panel of autoantigens, using the protocol that had enabled detection of responses in the 1st International NOD Mouse T-Cell Workshop. Under these assay conditions, 16 of 16 NOD mice displayed proliferative responses to whole GAD65, 13 of 16 to GAD(524-543), 9 of 16 to GAD(217-236), 7 of 16 to insulin(9-23), and 5 of 16 to HSP277. Thus, spontaneous proliferative T-cell responses can be consistently detected to some beta-cell autoantigens and peptides thereof. Overall, the results suggest that more sensitive assays (e.g., ELISPOT, ELISA analysis of cytokines in supernatants, or tetramer staining) may be preferred for the detection of autoreactive T-cells.
Assuntos
Autoimunidade , Camundongos Endogâmicos NOD/imunologia , Linfócitos T/imunologia , Animais , Autoantígenos/farmacologia , Divisão Celular/efeitos dos fármacos , Ensaio de Imunoadsorção Enzimática , Glutamato Descarboxilase/farmacologia , Proteínas de Choque Térmico/farmacologia , Técnicas Imunoenzimáticas , Insulina/farmacologia , Isoenzimas/farmacologia , Camundongos , Camundongos Endogâmicos BALB C , Fragmentos de Peptídeos/farmacologia , Linfócitos T/citologia , Linfócitos T/efeitos dos fármacosRESUMO
OBJECTIVE: To describe a probable association between TNDM and subsequent permanent IDDM. RESEARCH DESIGN AND METHODS: A longitudinal follow-up of a single case from birth to 12 yr of age was conducted analyzing sequential OGTTs, ICAs, AIAs, anti-GAD antibodies, and other organ-specific and nonspecific antibodies. RESULTS: A small-for-gestational-age infant developed hyperglycemia at 20 h of age and required insulin therapy for the 1st 14 wk of life (TNDM). Transient hyperglycemia and ketonuria were noted again at age 2 yr 10 mo during an intercurrent illness, but OGTT was normal; and ICA, AIA, anti-GAD65 and anti-GAD67 antibodies, antithyroid microsomal, anti-gastric parietal cell, antiadrenal, antisteroidal, and antinuclear antibodies were negative 3 wk later. At age 9 yr, hyperglycemia returned and persisted in the setting of hypoinsulinemia; ICA, AIA, anti-GAD65 and anti-GAD67 antibodies, and other organ-specific and nonspecific antibodies were again negative. Insulin therapy was initiated and has been maintained over 3 yr of follow-up. CONCLUSIONS: Our case is the fifth reported with permanent diabetes occurring after resolution of TNDM. The etiology of permanent diabetes in this setting is unknown but, unlike classical IDDM, appears unrelated to autoimmunity in our patient. The true frequency of this association remains unknown.
Assuntos
Autoanticorpos/sangue , Diabetes Mellitus Tipo 1/fisiopatologia , Anticorpos Anti-Insulina/sangue , Insulina/uso terapêutico , Biomarcadores/sangue , Diabetes Mellitus Tipo 1/sangue , Diabetes Mellitus Tipo 1/imunologia , Feminino , Seguimentos , Teste de Tolerância a Glucose , Glutamato Descarboxilase/sangue , Humanos , Recém-Nascido , Ilhotas Pancreáticas/imunologia , Estudos LongitudinaisRESUMO
We describe the presence of functional GABA(A) receptors on T cells. GABA inhibited anti-CD3 and antigen-specific T cell proliferation in vitro in a dose-dependent manner that was 1) mimicked by the GABA(A) receptor agonist muscimol (but not the GABA(B) receptor agonist baclofen), 2) blocked by GABA(A) receptor antagonists and a GABA(A) receptor Cl- channel blocker (picrotoxin) and 3) enhanced by pentobarbital. These data suggest that GABA(A) receptors mediate this immune inhibition and that these receptors can be modulated in a similar fashion to their neuronal counterparts. Finally, GABA inhibited DTH responses in vivo. Thus, pharmacological modulation of GABA(A) receptors may provide new approaches to modulate T cell responses in inflammation and autoimmune disease.
Assuntos
Hipersensibilidade Tardia , Receptores de GABA-A/imunologia , Linfócitos T/química , Linfócitos T/imunologia , Amidinas/farmacologia , Animais , Comunicação Autócrina/imunologia , Bicuculina/farmacologia , Complexo CD3/análise , Divisão Celular/imunologia , Inibidores Enzimáticos/farmacologia , Feminino , Agonistas GABAérgicos/farmacologia , Antagonistas GABAérgicos/farmacologia , Moduladores GABAérgicos/farmacologia , Terapia de Imunossupressão , Interleucina-2/biossíntese , Interleucina-2/imunologia , Ativação Linfocitária/efeitos dos fármacos , Ativação Linfocitária/imunologia , Masculino , Camundongos , Camundongos Endogâmicos NOD , Muscimol/farmacologia , Pentobarbital/farmacologia , Picrotoxina/farmacologia , Receptores de GABA-B/imunologia , Transdução de Sinais/imunologia , Linfócitos T/citologia , Vigabatrina , Ácido gama-Aminobutírico/análogos & derivados , Ácido gama-Aminobutírico/farmacologiaRESUMO
Repeated subconvulsive electrical stimulation of the amygdala leads to generalized seizures and provides an experimental model of epileptogenesis. Following electrical kindling stimulation the expression of c-Fos is rapidly induced. To evaluate the role of FOS protein in epileptogenesis, we used an antisense oligonucleotide strategy designed to inhibit its expression in the brain. Experimental and control oligonucleotides were delivered directly into the amygdala just prior to electrical stimulation. Immunocytochemical analysis showed that the administration of c-Fos antisense (but not sense) oligonucleotides inhibited expression of FOS in the amygdala following electrical stimulation. Behaviorally, treatment with c-Fos antisense oligonucleotides significantly accelerated the development of fully kindled (stage V) seizures. These data suggest that the increased FOS expression following electrical stimulation may be part of a protective mechanism which acts to inhibit epileptogenesis in the amygdala.
Assuntos
Tonsila do Cerebelo/efeitos dos fármacos , Epilepsia/fisiopatologia , Excitação Neurológica/fisiologia , Oligonucleotídeos Antissenso/farmacologia , Proteínas Proto-Oncogênicas c-fos/genética , Animais , Modelos Animais de Doenças , Estimulação Elétrica , Imuno-Histoquímica , Masculino , Oligonucleotídeos/farmacologia , Ratos , Ratos Wistar , Tionucleotídeos/farmacologiaRESUMO
In a previous study we demonstrated that increased FOS expression in the amygdala induced by partial kindling seizures could be attenuated by administering c-Fos specific antisense oligonucleotides. In addition, we found that the administration of c-Fos antisense oligonucleotides at the beginning of the amygdala kindling process facilitated the appearance of stage V kindled seizures. In the present study, we evaluated the effect of the suppression of FOS on the expression and severity of the generalized fully kindled seizures as well as on the susceptibility to additional ictal events during the postictal and interictal periods. We observed that the administration of c-Fos antisense oligonucleotides did not modify the behavioral and electrographic manifestations of generalized stage V kindled seizures. However, c-Fos antisense oligonucleotides significantly reduced the susceptibility to additional ictal events during the postictal refractory period. Hence, the increased FOS protein induced by generalized tonic clonic seizures may participate in postictal mechanisms.
Assuntos
Excitação Neurológica , Oligonucleotídeos Antissenso/uso terapêutico , Proteínas Proto-Oncogênicas c-fos/antagonistas & inibidores , Convulsões/tratamento farmacológico , Tonsila do Cerebelo/metabolismo , Animais , Excitação Neurológica/genética , Masculino , Proteínas Proto-Oncogênicas c-fos/metabolismo , Ratos , Ratos Wistar , Convulsões/metabolismoRESUMO
The results indicate that marked fluctuations in oxygen availability occur during the estrous cycle in both rats and hamsters. Patterns of luminal pO2 were similar in that levels were intermediate during metestrus and maximal during diestrus. By contrast, minimal pO2 levels occurred during estrus in the rat vs. during proestrus in the hamster. The species also differed in the range of mean pO2 occurring during the cycle: approximately 5-50 mmHg in the hamster vs. 25-50 mmHg in the rat. Ovariectomy results in marked increases in luminal pO2 in both species. The increase is reduced by hormone replacement.
Assuntos
Estro/metabolismo , Oxigênio/metabolismo , Útero/metabolismo , Animais , Cricetinae , Estrona/farmacologia , Feminino , Mesocricetus , Ovariectomia , Oxigênio/sangue , Progesterona/farmacologia , Ratos , Especificidade da Espécie , Útero/efeitos dos fármacosAssuntos
Autoanticorpos/análise , Autoantígenos/análise , Diabetes Mellitus Tipo 1/imunologia , Neuropatias Diabéticas/imunologia , Glutamato Descarboxilase/imunologia , Diabetes Mellitus Tipo 1/sangue , Diabetes Mellitus Tipo 1/enzimologia , Glutamato Descarboxilase/análise , Glutamato Descarboxilase/sangue , Humanos , Ilhotas Pancreáticas/imunologia , Proteínas Recombinantes/análise , Proteínas Recombinantes/imunologiaAssuntos
Síndrome da Imunodeficiência Adquirida/complicações , Pneumonia por Pneumocystis/etiologia , Técnicas de Laboratório Clínico , Humanos , Infecções Oportunistas/diagnóstico , Infecções Oportunistas/etiologia , Infecções Oportunistas/terapia , Pneumonia por Pneumocystis/diagnóstico , Pneumonia por Pneumocystis/terapiaRESUMO
When nonobese-diabetic (NOD) mouse embryos were implanted into pseudopregnant mothers of a nonautoimmune mouse strain, the progeny had a reduced type 1 diabetes (T1D) incidence, suggesting that transmission of maternal autoantibodies is important for T1D development. Whether eliminating islet autoantibody transmission in utero, or postnatally (through milk), prevented T1D is unknown. Herein, we show that fostering newborn NOD mice on B-cell deficient NOD.Igmu-/- dams does not prevent T1D, demonstrating that postnatally transmitted islet autoantibodies are not required for disease pathogenesis. Additionally, NOD.Igmu-/- mice reared on NOD dams did not develop T1D, indicating that autoantibody transmission to B-cell deficient NOD neonates is insufficient to trigger T1D. Interestingly, newborn NOD mice that were reared by ICR (but not NOD or C57BL/6) dams had reduced T1D incidence, although not as reduced as that reported after embryo transfer to ICR mice, suggesting that both prenatal and postnatal factors contribute to the observed reduction in T1D incidence. Thus, NOD mice have different risks for developing T1D depending on the strain of their foster mother, and both prenatal and postnatal maternal factors, other than islet autoantibodies, influence their T1D incidence. The results may be relevant for understanding the increasing incidence of T1D and designing interventions.