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1.
Cell ; 178(6): 1375-1386.e11, 2019 09 05.
Artigo em Inglês | MEDLINE | ID: mdl-31474366

RESUMO

In search of the molecular identities of cold-sensing receptors, we carried out an unbiased genetic screen for cold-sensing mutants in C. elegans and isolated a mutant allele of glr-3 gene that encodes a kainate-type glutamate receptor. While glutamate receptors are best known to transmit chemical synaptic signals in the CNS, we show that GLR-3 senses cold in the peripheral sensory neuron ASER to trigger cold-avoidance behavior. GLR-3 transmits cold signals via G protein signaling independently of its glutamate-gated channel function, suggesting GLR-3 as a metabotropic cold receptor. The vertebrate GLR-3 homolog GluK2 from zebrafish, mouse, and human can all function as a cold receptor in heterologous systems. Mouse DRG sensory neurons express GluK2, and GluK2 knockdown in these neurons suppresses their sensitivity to cold but not cool temperatures. Our study identifies an evolutionarily conserved cold receptor, revealing that a central chemical receptor unexpectedly functions as a thermal receptor in the periphery.


Assuntos
Proteínas de Caenorhabditis elegans/fisiologia , Caenorhabditis elegans/genética , Receptores de Glutamato/fisiologia , Receptores de Ácido Caínico/fisiologia , Receptores de Glutamato Metabotrópico/fisiologia , Sensação Térmica/fisiologia , Animais , Células CHO , Proteínas de Caenorhabditis elegans/genética , Temperatura Baixa , Cricetulus , Humanos , Camundongos , Neurônios/metabolismo , Receptores de Glutamato/genética , Receptores de Ácido Caínico/genética , Receptores de Glutamato Metabotrópico/genética , Sensação Térmica/genética
2.
Biomed Eng Online ; 22(1): 81, 2023 Aug 18.
Artigo em Inglês | MEDLINE | ID: mdl-37596652

RESUMO

BACKGROUND: In this study, we proposed a deep learning technique that can simultaneously detect suspicious positions of benign vocal cord tumors in laparoscopic images and classify the types of tumors into cysts, granulomas, leukoplakia, nodules and polyps. This technique is useful for simplified home-based self-prescreening purposes to detect the generation of tumors around the vocal cord early in the benign stage. RESULTS: We implemented four convolutional neural network (CNN) models (two Mask R-CNNs, Yolo V4, and a single-shot detector) that were trained, validated and tested using 2183 laryngoscopic images. The experimental results demonstrated that among the four applied models, Yolo V4 showed the highest F1-score for all tumor types (0.7664, cyst; 0.9875, granuloma; 0.8214, leukoplakia; 0.8119, nodule; and 0.8271, polyp). The model with the lowest false-negative rate was different for each tumor type (Yolo V4 for cysts/granulomas and Mask R-CNN for leukoplakia/nodules/polyps). In addition, the embedded-operated Yolo V4 model showed an approximately equivalent F1-score (0.8529) to that of the computer-operated Yolo-4 model (0.8683). CONCLUSIONS: Based on these results, we conclude that the proposed deep-learning-based home screening techniques have the potential to aid in the early detection of tumors around the vocal cord and can improve the long-term survival of patients with vocal cord tumors.


Assuntos
Cistos , Prega Vocal , Humanos , Redes Neurais de Computação , Simulação por Computador , Leucoplasia
3.
Dermatol Ther ; 35(2): e15240, 2022 02.
Artigo em Inglês | MEDLINE | ID: mdl-34854186

RESUMO

Cryotherapy (or cryosurgery) has been performed to treat various skin lesions in the field of dermatology; however, to the best of our knowledge, no study has investigated its efficacy and safety for benign pigmented lesions. Therefore, we conducted a split-face study to evaluate the efficacy and safety of cryotherapy in the treatment of benign pigmented lesions. A total of five subjects were included. Picosecond laser therapy was performed to treat the whole face and cryotherapy for half the face. Four weeks after completing the treatment sessions, patients showed more clinical improvement on the laser and cryotherapy combination treatment side than on the laser-only side, with no adverse events. Our study demonstrated that cryotherapy is a potential adjuvant therapeutic modality for benign pigmented lesions.


Assuntos
Lasers de Estado Sólido , Neodímio , Alumínio , Crioterapia/efeitos adversos , Humanos , Lasers de Estado Sólido/uso terapêutico , Resultado do Tratamento , Ítrio
4.
Nano Lett ; 21(1): 337-343, 2021 01 13.
Artigo em Inglês | MEDLINE | ID: mdl-33227203

RESUMO

Since the emergence of the COVID-19 pandemic outbreak, the increasing demand and disposal of surgical masks has resulted in significant economic costs and environmental impacts. Here, we applied a dual-channel spray-assisted nanocoating hybrid of shellac/copper nanoparticles (CuNPs) to a nonwoven surgical mask, thereby increasing the hydrophobicity of the surface and repelling aqueous droplets. The resulting surface showed outstanding photoactivity (combined photocatalytic and photothermal properties) for antimicrobial action, conferring reusability and self-sterilizing ability to the masks. Under solar illumination, the temperature of this photoactive antiviral mask (PAM) rapidly increased to >70 °C, generating a high level of free radicals that disrupted the membrane of nanosized (∼100 nm) virus-like particles and made the masks self-cleaning and reusable. This PAM design can provide significant protection against the transmission of viral aerosols in the fight against the COVID-19 pandemic.


Assuntos
Antivirais/química , COVID-19/prevenção & controle , Cobre/química , Máscaras/virologia , Nanopartículas Metálicas/química , Esterilização/métodos , Catálise , Humanos , Interações Hidrofóbicas e Hidrofílicas , Processos Fotoquímicos , SARS-CoV-2/isolamento & purificação , Temperatura
5.
Anal Chem ; 93(42): 14169-14177, 2021 10 26.
Artigo em Inglês | MEDLINE | ID: mdl-34644049

RESUMO

Diffusioosmosis (DO) results from ion transport near charged surfaces in the presence of electrolyte gradients and is critical in nanofluidic systems. However, DO has not yet been comprehensively studied because nanofabrication materials have limitations of low throughput and difficult quantification. Herein, we describe a self-assembled particle membrane (SAPM)-integrated microfluidic platform that can modulate the material properties (e.g., zeta-potential) and transport flux of nanopores. We quantify the effect of the zeta-potential on DO by measuring the electrical signals across three different nanopores/nanochannels of the SAPM. We then empirically quantify the effects of the temperature and ionic strength of the electrolytes on DO and reveal a nonlinear relationship with DO-driven ion transport; the ionic strengths govern the DO- or diffusion-effective ion transport phenomena. Finally, we demonstrate DO-driven electric power generation with enhanced performance as a potential application under optimized experimental conditions.


Assuntos
Nanoporos , Difusão , Eletrólitos , Transporte de Íons , Temperatura
6.
Biomed Eng Online ; 20(1): 51, 2021 May 25.
Artigo em Inglês | MEDLINE | ID: mdl-34034766

RESUMO

BACKGROUND: Early detection of laryngeal masses without periodic visits to hospitals is essential for improving the possibility of full recovery and the long-term survival ratio after prompt treatment, as well as reducing the risk of clinical infection. RESULTS: We first propose a convolutional neural network model for automated laryngeal mass detection based on diagnostic images captured at hospitals. Thereafter, we propose a pilot system, composed of an embedded controller, a camera module, and an LCD display, that can be utilized for a home-based self-screening test. In terms of evaluating the model's performance, the experimental results indicated a final validation loss of 0.9152 and a F1-score of 0.8371 before post-processing. Additionally, the F1-score of the original computer algorithm with respect to 100 randomly selected color-printed test images was 0.8534 after post-processing while that of the embedded pilot system was 0.7672. CONCLUSIONS: The proposed technique is expected to increase the ratio of early detection of laryngeal masses without the risk of clinical infection spread, which could help improve convenience and ensure safety of individuals, patients, and medical staff.


Assuntos
Redes Neurais de Computação , Diagnóstico por Computador
7.
Biomed Eng Online ; 18(1): 100, 2019 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-31619241

RESUMO

BACKGROUND: It is crucial to frequently inspect the proper operation of non-invasive electronic blood pressure monitors in various sites to prevent accidents from inaccurate blood pressure measurements, especially for large-scale hospitals. However, most conventional blood pressure monitor inspection devices are not suitable for such on-site investigation purpose. In this study, we propose a new single-pieced, fully air-driven pseudo blood pressure generator that is suitable for frequent on-site pre-screening tests of the blood pressure monitor by nurses. RESULTS: The proposed model comprises a rigid cylindrical body, two simulated brachial arteries, two air-pumps, an electronic controller, and a pressure sensor. Control algorithm based on polynomial curve fitting was implemented to generate various user-instructed systolic blood pressure and heart-rate conditions automatically. To evaluate the performance and clinical feasibility of the proposed model, various experiments were performed using ten commercial electronic blood pressure monitors. Experimental results demonstrated that the values of the Pearson coefficient between the reference pseudo-blood pressure waveforms and the actually generated pressure waveforms were 0.983, 0.983 and 0.997 at 60, 70 and 80 beats/min, respectively (p < 0.05). Besides, during the experiments using ten commercial blood pressure monitors, the maximum error in average systolic blood pressure was 2.9 mmHg, the maximum standard deviation in average systolic blood pressure was 3.5 mmHg, and the maximum percentage error in average pumping rate was 3.2%, respectively. CONCLUSIONS: We expect that the proposed model can give an easy and comprehensive way for frequent on-site investigations of the blood pressure monitors by nurses, and improve the safety of patients with abnormal blood pressure, especially in most large-scale hospitals.


Assuntos
Ar , Monitores de Pressão Arterial , Enfermeiras e Enfermeiros , Estudos de Viabilidade , Projetos de Pesquisa
8.
Nat Mater ; 14(3): 295-300, 2015 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-25419813

RESUMO

Thermal conductivity is an important property for polymers, as it often affects product reliability (for example, electronics packaging), functionality (for example, thermal interface materials) and/or manufacturing cost. However, polymer thermal conductivities primarily fall within a relatively narrow range (0.1-0.5 W m(-1) K(-1)) and are largely unexplored. Here, we show that a blend of two polymers with high miscibility and appropriately chosen linker structure can yield a dense and homogeneously distributed thermal network. A sharp increase in cross-plane thermal conductivity is observed under these conditions, reaching over 1.5 W m(-1) K(-1) in typical spin-cast polymer blend films of nanoscale thickness, which is approximately an order of magnitude larger than that of other amorphous polymers.

9.
Proc Inst Mech Eng H ; 238(1): 45-54, 2024 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-38044662

RESUMO

Owing to environmental and disease issues, the use of high-frequency chest wall oscillation (HFCWO) devices in hospitals is consistently increasing. This study proposes a cost-effective actuator-less HFCWO device that utilizes an external wall port utility in hospitals to generate the positive and negative pneumatic pressures required for HFCWO treatment instead of an embedded mechanical actuator. The manufactured prototype with the no-amplification (NO-AMP) setting contained an electric pressure regulator to enable intensity level adjustment and two solenoid valves to enable vibration frequency adjustment, whereas the prototype with the pre-amplification (PRE-AMP) setting contained an additional air reservoir and an air-pressure booster. The prototype device was tuned to output average local maximum values in the pressure waveform similar to a commercial VEST-205 device at an 8-12 Hz frequency and 2-4 pressure intensity levels. In vitro comparative experiments demonstrated that the prototype device showed similar local maximum pressures to those of the VEST-205 (mean absolute pressure difference, <3 mmH2O); in contrast, the proposed device showed significantly higher local minimum pressures than those of the VEST-205 (mean absolute pressure difference, >8 mmH2O). Additionally, the driving sound of the proposed device was 17.0-17.8 dB higher than that of VEST-205. We conclude that the proposed device has the potential to substitute for conventional HFCWO devices under the limited but most frequently used operating conditions, although more detailed modifications are necessary in future studies to improve its performance and clinical usability.


Assuntos
Oscilação da Parede Torácica , Pressão
10.
Biomed Eng Lett ; : 1-10, 2023 Jun 07.
Artigo em Inglês | MEDLINE | ID: mdl-37360627

RESUMO

Accidents related to the administration of intravenous (IV) medication, such as drug overdose/underdose, drug/patient mis-identification, and delayed bag exchange, occur consistently in clinical fields. Several previous studies have suggested various contact-sensing and image-processing methodologies; however, most of them can increase the workload of nursing staffs during the long-term, continuous monitoring. In this study, we proposed a smart IV pole that can monitor the infusion status of up to four IV medications (patient/drug identification, and liquid residue) with various sizes and hanging positions to reduce IV-related accidents and improve patient safety with the least additional workload; the system consists of 12 cameras, one code scanner, and four controllers. Two types of deep learning models for automated camera selection (CNN-1) and liquid residue monitoring (CNN-2), and three drug residue estimation equations were implemented. The experimental results demonstrated that the accuracy of identification code-checking (60 tests) was 100%. The classification accuracy and the mean inference time of CNN-1 (1200 tests) were 100% and 140 ms. The mean average precision and the mean inference time of CNN-2 (300 tests) were 0.94 and 144 ms. The average error rates between the alarm setting (20, 30, and 40 mL) and the actual drug residue when the alarm first generated were 4.00%, 7.33%, and 4.50% for a 1,000 mL bag; 6.00%, 4.67%, and 2.50% for a 500 mL bag; and 3.00%, 6.00%, and 3.50% for a 100 mL bag, respectively. Our results suggest that the implemented AI-based prototype IV pole is a potential tool for reducing IV-related accidents and improving in-hospital patient safety. Supplementary Information: The online version contains supplementary material available at 10.1007/s13534-023-00292-w.

11.
Curr Biol ; 33(18): 3985-3991.e4, 2023 09 25.
Artigo em Inglês | MEDLINE | ID: mdl-37643623

RESUMO

Despite lacking ears, the nematode C. elegans senses airborne sound and engages in phonotaxis behavior, enabling it to locate and avoid sound sources.1 How worms sense sound, however, is not well understood. Here, we report an interesting observation that worms respond only to sounds emitted by small but not large speakers, indicating that they preferentially respond to localized sound sources. Notably, sounds emitted by small speakers form a sharp sound pressure gradient across the worm body, while sounds from large speakers do not, suggesting that worms sense sound pressure gradients rather than absolute sound pressure. Analysis of phonotaxis behavior, sound-evoked skin vibration, and sound-sensitive neuron activities further support this model. We suggest that the ability to sense sound pressure gradients provides a potential mechanism for worms to distinguish sounds generated by their predators, which are typically small animals, from those produced by large animals or background noise. As vertebrate cochlea and some insect ears can also detect sound pressure gradients, our results reveal that sensing of sound pressure gradients may represent a common mechanism in auditory sensation across animal phyla. VIDEO ABSTRACT.


Assuntos
Caenorhabditis elegans , Som , Animais , Vibração , Ruído , Cóclea
12.
J Cosmet Dermatol ; 22(12): 3375-3378, 2023 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-37218601

RESUMO

BACKGROUND: In any dermatologic procedure, patient acceptance of treatment is heavily influenced by intraprocedural pain. Intralesional triamcinolone injections are very important in keloid scar and nodulocystic acne treatment. However, the main problem of needle-stick procedures is pain. Cryoanesthesia is ideally intended to cool only the epidermis during treatment and has advantage which did not require application time. AIMS: The aim of this study was to investigate the pain-reducing effect and safety of CryoVIVE® (newly introduced cryoanesthesia device) during triamcinolone injections for nodulocystic acne in actual clinical settings. PATIENTS/METHODS: In this two-staged, non-randomized clinical trial, a total of 64 subjects underwent intralesional triamcinolone injections for their acne lesions with cold anesthesia using CryoVIVE®. The pain intensity was assessed with Visual Analogue Scale (VAS) scores. Safety profile was also evaluated. RESULTS: The mean pain VAS scores on the lesion with and without cold anesthesia were 3.667 and 5.933, respectively (p = 0.0001). No side effects, discoloration, and scarring were observed. CONCLUSION: In conclusion, the anesthetic use of CryoVIVE® with intralesional corticosteroid injections is a practical and well-tolerated modality.


Assuntos
Acne Vulgar , Anestesia , Queloide , Humanos , Triancinolona Acetonida , Dor/prevenção & controle , Dor/induzido quimicamente , Queloide/tratamento farmacológico , Injeções Intralesionais , Acne Vulgar/complicações , Acne Vulgar/tratamento farmacológico , Esteroides , Resultado do Tratamento
13.
Indian J Dermatol ; 67(2): 204, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36092232

RESUMO

Background: Pigmentary skin disorders impair the quality of life, leading to the development of therapeutic modalities. However, these treatments should focus more on effectiveness and safety. Aims and Objectives: To evaluate the effect of a temperature-adjustable cryotherapy device on the expression of pigmentation-related biomarkers. Methods and Results: A temperature- and time-adjustable cryotherapy device was employed to improve 200 mJ UVB-induced pigmentation on mice at -5°C (for 5, 10 or 20 s), 0°C (for 5, 10 or 20 s), 5°C (for 5, 10 or 20 s), or 10°C (for 5, 10 or 20 s). Expression of pigmentation-related biomarkers, such as tyrosinase, c-kit, melanocortin 1 receptor and microphthalmia-associated transcription factor before and after treatment with the cryotherapy device was investigated with real-time polymerase chain reaction and immunohistochemistry. Results: Expression of pigmentation-related biomarkers was decreased after the treatment of the temperature-adjustable cryotherapy device. Gene expression of the pigmentation-related biomarkers was decreased under the above conditions with some exception. Protein expression of the pigmentation-related biomarkers showed decreased tendency under the conditions with some exceptions. Conclusion: The temperature-adjustable cryotherapy device used in this study reduced the expression of pigmentation-related biomarkers on mice and may be used to treat patients with skin pigmentation.

14.
Clin Ophthalmol ; 15: 4659-4666, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34916777

RESUMO

PURPOSE: To evaluate the safety and efficacy of a novel medical device to provide cooling anesthesia to the eye as local anesthesia for intravitreal injections. STUDY DESIGN: First in human, open-label study of 43 subjects assessed at three different doses: -10°C for 20 seconds (group 1), -15°C for 15 seconds (group 2), and -15°C for 20 seconds (group 3). Main outcome measures were safety and pain of injection using a numeric rating scale (NRS). RESULTS: Cooling anesthesia did not result in any serious ocular adverse events. One grade 1 adverse event was a vasovagal response during cooling administration which resolved immediately after cooling. Mean NRS scores at the time of injection for each group ranged from 2.5 to 4.3 There was a statistically significant difference between pain scores of the 3 groups at injection in aggregate but not in pairwise comparisons (P value = 0.047). There was a statistically significant decrease in pain from injection to 5 minutes post injection in all groups (P value = 0.00008, 0.003, 0.0005 for groups 1, 2, 3, respectively) as well as from 5 minutes to 24-48 hours (P value = 0.00001, 0.018, and 0.0545 for groups 1, 2, 3, respectively). CONCLUSION: The rapid cooling anesthesia device was well tolerated for achieving local anesthesia among patients receiving intravitreal injections with no serious ocular adverse events.

15.
Proc Inst Mech Eng H ; 235(5): 566-573, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33615902

RESUMO

For effective patient therapy and improved patient safety, it is critical to administer medication accurately in accordance with doctor's prescription. However, accidents owing to the erroneous programing of infusion pumps caused by users have been consistently reported in several documents. In this study, the authors propose a novel surveillance technique for infusion pumps to continuously monitor the variations in panel digits using a convolutional neural network model, and evaluate the performance of the implemented technique. During the experimental evaluation, 1st-step ROIs and 2nd-step ROIs were successfully extracted from the frame images regardless of the ambient lighting conditions. The final accuracies of the implemented CNN model are 99.9% for both the training (172,800 images) and validation (1080 images) dataset while the final losses for the training and validation datasets are 0.48 and 0.45 after 13th epoch, respectively. In the 24-h continuous monitoring test, the accuracy of the model for volume recognition considering all the 1440 measurements (960 for day-lighting and 480 for night-lighting) is 95.5%, whereas in day-lighting and night-lighting modes the accuracies of the model are 98.2% and 90.0%, respectively. Based on these experimental results, the proposed surveillance technique incorporating infusion pumps is expected to improve the safety of patients who need long-term treatments via infusion pumps, reducing the burden on the nurses and hospitals.


Assuntos
Bombas de Infusão , Redes Neurais de Computação , Humanos , Iluminação
16.
Proc Inst Mech Eng H ; 235(1): 73-81, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32972310

RESUMO

Intravenous (IV) medication administration processes have been considered as high-risk steps, because accidents during IV administration can lead to serious adverse effects, which can deteriorate the therapeutic effect or threaten the patient's life. In this study, we propose a multi-modal infusion pump (IP) monitoring technique, which can detect mismatches between the IP setting and actual infusion state and between the IP setting and doctor's prescription in real time using a thin membrane potentiometer and convolutional-neural-network-based deep learning technique. During performance evaluation, the percentage errors between the reference infusion rate (IR) and average estimated IR were in the range of 0.50-2.55%, while those between the average actual IR and average estimated IR were in the range of 0.22-2.90%. In addition, the training, validation, and test accuracies of the implemented deep learning model after training were 98.3%, 97.7%, and 98.5%, respectively. The training and validation losses were 0.33 and 0.36, respectively. According to these experimental results, the proposed technique could provide improved protection functions to IV-administration patients.


Assuntos
Bombas de Infusão , Redes Neurais de Computação , Humanos , Infusões Intravenosas
17.
Polymers (Basel) ; 13(4)2021 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-33670700

RESUMO

Functionalized graphene-polymer nanocomposites have gained significant attention for their enhanced mechanical, thermal, and antibacterial properties, but the requirement of multi-step processes or hazardous reducing agents to functionalize graphene limits their current applications. Here, we present a single-step synthesis of thermally reduced graphene oxide (TrGO) based on shellac, which is a low-cost biopolymer that can be employed to produce poly(vinyl alcohol) (PVA)/TrGO nanocomposites (PVA-TrGO). The concentration of TrGO varied from 0.1 to 2.0 wt.%, and the critical concentration of homogeneous TrGO dispersion was observed to be 1.5 wt.%, below which strong interfacial molecular interactions between the TrGO and the PVA matrix resulted in improved thermal and mechanical properties. At 1.5 wt.% filler loading, the tensile strength and modulus of the PVA-TrGO nanocomposite were increased by 98.7% and 97.4%, respectively, while the storage modulus was increased by 69%. Furthermore, the nanocomposite was 96% more effective in preventing bacterial colonization relative to the neat PVA matrix. The present findings indicate that TrGO can be considered a promising material for potential applications in biomedical devices.

18.
Sci Adv ; 7(1)2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33523863

RESUMO

We report a versatile method to make liquid metal composites by vigorously mixing gallium (Ga) with non-metallic particles of graphene oxide (G-O), graphite, diamond, and silicon carbide that display either paste or putty-like behavior depending on the volume fraction. Unlike Ga, the putty-like mixtures can be kneaded and rolled on any surface without leaving residue. By changing temperature, these materials can be stiffened, softened, and, for the G-O-containing composite, even made porous. The gallium putty (GalP) containing reduced G-O (rG-O) has excellent electromagnetic interference shielding effectiveness. GalP with diamond filler has excellent thermal conductivity and heat transfer superior to a commercial liquid metal-based thermal paste. Composites can also be formed from eutectic alloys of Ga including Ga-In (EGaIn), Ga-Sn (EGaSn), and Ga-In-Sn (EGaInSn or Galinstan). The versatility of our approach allows a variety of fillers to be incorporated in liquid metals, potentially allowing filler-specific "fit for purpose" materials.

19.
Mater Sci Eng C Mater Biol Appl ; 108: 110465, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31924022

RESUMO

A biocompatible, cost-effective, and scalable reduced graphene oxide (rGO) film was obtained from shellac using thermal treatment and its structural, chemical, and electrical properties were investigated. This thermally-decomposed rGO (TrGO) film exhibited good crystallinity, low sheet resistance, and high carbon content. TrGO flakes obtained from the film were dispersed and drop cast onto indium tin oxide/glass electrodes to fabricate label-free electrochemical immunosensors for the quantitative detection of the influenza virus H1N1 via electrochemical impedance spectroscopy. These sensors exhibited high stability and reproducibility, both possibly ascribable to the high adhesion of TrGO due to its phenolic-OH moiety; the limits of detection were 26 and 33 plaque-forming units, respectively, in phosphate-buffered saline and diluted saliva. These cost-effective TrGO-based sensors showed great potential as reliable and robust nanomaterial-based biosensors for widespread clinical applications.


Assuntos
Técnicas Biossensoriais , Espectroscopia Dielétrica , Grafite/química , Vírus da Influenza A Subtipo H1N1 , Compostos de Estanho/química , Limite de Detecção
20.
Proc Inst Mech Eng H ; 234(4): 370-376, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31872782

RESUMO

Medication infusion pumps are the most popular device in almost all areas of a hospital; therefore, it is important to frequently inspect the accuracy of the infusion pump operation to prevent underdose/overdose accidents. However, the conventional infusion pump inspection devices are not suitable for quick and convenient on-site inspection by nurses. In this study, a new IR estimation technique for peristaltic infusion pumps that facilitates on-site pre-screening test with shorter inspection time was proposed. A thin membrane potentiometer was attached to a catheter and the actual IR was estimated based on a time interval between two successive line pushes of an identical cam follower using power function estimation. To evaluate the performance of the proposed IR estimation technique, in vitro experiments were performed using 11 infusion pumps (three for Infusion Pump SET 1 (IPSET-1) and eight for Infusion Pump SET 2 (IPSET-2)) with the same model. In experiments, error rate between the actual and the measured values (using conventional inspection device) were 0.04-1.17% range for IPSET-1 and 2.09-4.32% for IPSET-2, and those between the actual and the estimated values (using proposed method) were 0.02-0.62% range for IPSET-1 and 1.31-4.23% for IPSET-2. The proposed technique had almost equivalent performance with a commercial inspection device, but the time for inspection was reduced to almost one third. We expect that the proposed technique can provide a tool for simple and convenient on-site pre-screening of infusion pumps by nurses to improve patient safety.


Assuntos
Bombas de Infusão , Enfermeiras e Enfermeiros , Injeções , Cinética
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