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1.
Small ; : e2407262, 2024 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-39308255

RESUMO

Aqueous Zn-ion batteries are promising and efficient energy storage systems owing to their low cost, high safety, and satisfactory capacity. However, the instability of Zn metal anodes, caused by dendritic growth and parasitic side reactions, hinders their practical application. In this study, a nanophase-separated block copolymer layer that enhances the reversibility of Zn metal anodes is introduced. This layer consists of two components: a high-performance engineering-plastic-based hydrophobic block exhibiting excellent mechanical properties and chemical stability, and a hydrophilic block that significantly improves the interfacial stability of the anode by selectively permeating Zn ions through the separated nanophase channels. Through an improved electrochemical system and scalable fabrication process, this block copolymer provides a feasible approach for the practical application of Zn metal anodes in aqueous energy storage systems.

2.
Nano Lett ; 23(22): 10391-10397, 2023 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-37943575

RESUMO

Lithium-sulfur batteries (LiSBs) are promising next-generation batteries because of their low cost and high theoretical energy densities. Despite remarkable advances over the decades, polysulfide (PS) shuttling during battery cycling remains a challenge in the development of commercial LiSBs and is accelerated under practical conditions. Herein, we report a permselective ionic shield between the electrodes that blocks PS shuttles and passes Li ions to high-performance LiSBs. This shield is easily built onto the separator by ionic complexation and intermolecular bonding of functional polymers, thereby improving the battery performance and safety. The LiSB with the developed shield delivers a remarkable discharge capacity of 917 mAh g-1 after 1000 cycles at 2 C. In addition, the behavior of LiSBs under practical conditions that can realize a high energy density is investigated to achieve the optimal balance in this system. This study provides new insights into the imminent development of separators for practical LiSBs.

3.
Mol Ther ; 30(8): 2664-2679, 2022 08 03.
Artigo em Inglês | MEDLINE | ID: mdl-35690907

RESUMO

Recessive dystrophic epidermolysis bullosa (RDEB) is a severe skin fragility disorder caused by loss-of-function mutations in the COL7A1 gene, which encodes type VII collagen (C7), a protein that functions in skin adherence. From 36 Korean RDEB patients, we identified a total of 69 pathogenic mutations (40 variants without recurrence), including point mutations (72.5%) and insertion/deletion mutations (27.5%). For fibroblasts from two patients (Pat1 and Pat2), we applied adenine base editors (ABEs) to correct the pathogenic mutation of COL7A1 or to bypass a premature stop codon in Pat1-derived primary fibroblasts. To expand the targeting scope, we also utilized prime editors (PEs) to correct the COL7A1 mutations in Pat1- and Pat2-derived fibroblasts. Ultimately, we found that transfer of edited patient-derived skin equivalents (i.e., RDEB keratinocytes and PE-corrected RDEB fibroblasts from the RDEB patient) into the skin of immunodeficient mice led to C7 deposition and anchoring fibril formation within the dermal-epidermal junction, suggesting that base editing and prime editing could be feasible strategies for ex vivo gene editing to treat RDEB.


Assuntos
Colágeno Tipo VII , Epidermólise Bolhosa Distrófica , Animais , Colágeno Tipo VII/genética , Colágeno Tipo VII/metabolismo , Epidermólise Bolhosa Distrófica/genética , Epidermólise Bolhosa Distrófica/patologia , Epidermólise Bolhosa Distrófica/terapia , Genes Recessivos , Queratinócitos/metabolismo , Camundongos , Mutação , Pele/metabolismo
4.
Pediatr Dermatol ; 40(6): 1149-1151, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37170713

RESUMO

Epidermolytic ichthyosis (EI) is a rare genetic disorder of keratinization caused by mutations in either KRT1 or KRT10. Histopathologically, epidermolytic hyperkeratosis (EHK) is a hallmark of EI. Here, we report two EI cases in which KRT1 mutation was confirmed by molecular study, but without typical EHK present on skin biopsies performed within 1 week of age. Our cases demonstrate that EHK may not be evident in EI if skin biopsy is performed during the neonatal period.


Assuntos
Hiperceratose Epidermolítica , Recém-Nascido , Humanos , Hiperceratose Epidermolítica/diagnóstico , Hiperceratose Epidermolítica/genética , Mutação , Pele/patologia , Biópsia , Queratina-1/genética
5.
Proc Natl Acad Sci U S A ; 117(2): 1216-1222, 2020 01 14.
Artigo em Inglês | MEDLINE | ID: mdl-31889001

RESUMO

Certain long-distance migratory animals, such as salmon and sea turtles, are thought to imprint on the magnetic field of their natal area and to use this information to help them return as adults. Despite a growing body of indirect support for such imprinting, direct experimental evidence thereof remains elusive. Here, using the fruit fly as a magnetoreceptive model organism, we demonstrate that exposure to a specific geographic magnetic field during a critical period of early development affected responses to a matching magnetic field gradient later in life. Specifically, hungry flies that had imprinted on a specific magnetic field from 1 of 3 widely separated geographic locations responded to the imprinted field, but not other magnetic fields, by moving downward, a geotactic behavior associated with foraging. This same behavior occurred spontaneously in the progeny of the next generation: female progeny moved downward in response to the field on which their parents had imprinted, whereas male progeny did so only in the presence of these females. These results represent experimental evidence that organisms can learn and remember a magnetic field to which they were exposed during a critical period of development. Although the function of the behavior is not known, one possibility is that imprinting on the magnetic field of a natal area assists flies and their offspring in recognizing locations likely to be favorable for foraging and reproduction.


Assuntos
Migração Animal/fisiologia , Drosophila/fisiologia , Campos Magnéticos , Animais , Feminino , Comportamento de Retorno ao Território Vital/fisiologia , Fixação Psicológica Instintiva/fisiologia , Masculino , Reprodução
6.
Nano Lett ; 22(24): 10176-10183, 2022 Dec 28.
Artigo em Inglês | MEDLINE | ID: mdl-36524871

RESUMO

The lithium-sulfur battery is one of the most promising "beyond Li-ion" battery chemistries owing to its superior gravimetric energy density and low cost. Nonetheless, its commercialization has been hindered by its low cycle life due to the polysulfide shuttle and nonuniform Li-metal plating and stripping. Thin and dense solid electrolyte separators could address these issues without compromising on energy density. Here, we introduce a novel argyrodite (Li6PS5Cl)-carboxylated nitrile butadiene rubber (XNBR) composite thin solid electrolyte separator (TSE) (<50 µm) processed by a scalable calendering technique and compatible with Li-metal. When integrated in a full cell with a commercial tape-cast sulfur cathode (3.54 mgS cm-2) in the presence of an in situ polymerized lithium bis(fluorosulfonyl)imide-polydioxolane catholyte and a 100 µm Li-metal foil anode, we demonstrate stable cycling for 50 cycles under realistic operating conditions (stack pressure of <1 MPa and 30 °C).

7.
Neuropsychol Rev ; 32(2): 193-227, 2022 06.
Artigo em Inglês | MEDLINE | ID: mdl-33959887

RESUMO

Alzheimer's disease (AD) is the leading cause of dementia, and mild cognitive impairment (MCI) is considered the transitional state to AD dementia (ADD) and other types of dementia, whose symptoms are accompanied by altered eye movement. In this work, we reviewed the existing literature and conducted a meta-analysis to extract relevant eye movement parameters that are significantly altered owing to ADD and MCI. We conducted a systematic review of 35 eligible original publications in saccade paradigms and a meta-analysis of 27 articles with specified task conditions, which used mainly gap and overlap conditions in both prosaccade and antisaccade paradigms. The meta-analysis revealed that prosaccade and antisaccade latencies and frequency of antisaccade errors showed significant alterations for both MCI and ADD. First, both prosaccade and antisaccade paradigms differentiated patients with ADD and MCI from controls, however, antisaccade paradigms was more effective than prosaccade paradigms in distinguishing patients from controls. Second, during prosaccade in the gap and overlap conditions, patients with ADD had significantly longer latencies than patients with MCI, and the trend was similar during antisaccade in the gap condition as patients with ADD had significantly more errors than patients with MCI. The anti-effect magnitude was similar between controls and patients, and the magnitude of the latency of the gap effect varied among healthy controls and MCI and ADD subjects, but the effect size of the latency remained large in both patients. These findings suggest that, using gap effect, anti-effect, and specific choices of saccade paradigms and conditions, distinctions could be made between MCI and ADD patients as well as between patients and controls.


Assuntos
Doença de Alzheimer , Disfunção Cognitiva , Doença de Alzheimer/diagnóstico , Disfunção Cognitiva/diagnóstico , Humanos , Movimentos Sacádicos
8.
Chem Eng J ; 430: 132723, 2022 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-34629939

RESUMO

COVID-19 is a pandemic that has caused serious disruption in almost every day-to-day life around the world, and wearing a mask is essential for human safety from this virus. However, masks are non-recyclable materials, and the accumulation of masks used every day causes serious environmental issues. In this study, we investigate the recycling of mask materials for addressing the environmental problems and transforming as a high value-added material through chemical modification of masks. The recycled mask is applied as a separator for aqueous rechargeable batteries, and shows outstanding safety and electrochemical performance than the existing separator. This approach will lead to an advanced energy technology considering nature after overcoming COVID-19.

9.
FASEB J ; 34(2): 3289-3304, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-31916636

RESUMO

The enzyme glutathione S-transferase theta 1 (GSTT1) is involved in detoxifying chemicals, including reactive oxygen species (ROS). Here, we provide a significant insight into the role of GSTT1 in inflammatory bowel disease (IBD). We identified decreased expression of GSTT1 in inflamed colons from IBD patients compared to controls. We intrarectally or intraperitoneally delivered Gstt1 gene to mice with dextran sodium sulfate (DSS)-induced colitis and noted attenuation of colitis through gene transfer of Gstt1 via an IL-22 dependent pathway. Downregulation of GSTT1 by pathogen-associated molecular patterns (PAMPs) of microbes reduced innate defense responses and goblet cell differentiation. The GSTT1 mutation in intestinal epithelial cells (IECs) and IBD patients decreased its dimerization, which was connected to insufficient phosphorylation of signal transducer and activator of transcription-3 and p38/mitogen-activated protein kinase by their common activator, IL-22. GSTT1 ameliorated colitis and contributed as a modulator of goblet cells through sensing pathogens and host immune responses. Its mutations are linked to chronic intestinal inflammation due to its insufficient dimerization. Our results provide new insights into GSTT1 mutations that are linked to chronic intestinal inflammation due to its insufficient dimerization and their functional consequences in IBDs.


Assuntos
Diferenciação Celular , Colite Ulcerativa/metabolismo , Glutationa Transferase/metabolismo , Células Caliciformes/metabolismo , Interleucinas/metabolismo , Animais , Células Cultivadas , Colite Ulcerativa/genética , Colite Ulcerativa/patologia , Enterócitos/citologia , Enterócitos/metabolismo , Feminino , Glutationa Transferase/genética , Células Caliciformes/citologia , Células HT29 , Humanos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Multimerização Proteica , Fator de Transcrição STAT3/genética , Fator de Transcrição STAT3/metabolismo , Células THP-1 , Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo , Interleucina 22
10.
J Allergy Clin Immunol ; 146(5): 1070-1079.e8, 2020 11.
Artigo em Inglês | MEDLINE | ID: mdl-32311391

RESUMO

BACKGROUND: Pemphigus vulgaris (PV) is an autoimmune bullous disease mediated by autoantibodies against desmoglein 3 (DSG3). Inducible costimulator (ICOS) is a costimulatory receptor expressed on T cells and influences the activity of T follicular helper (TFH) cells in various autoimmune diseases, but the roles of ICOS and TFH cells in PV remain unclear. OBJECTIVE: We examined the immunological characteristics, antigen specificity, and pathogenicity of CD4+ T-cell subpopulations, as well as the therapeutic effect of anti-ICOS blocking antibodies in PV. METHODS: A mouse model of PV was established by adoptive transfer of immune cells from the skin-draining lymph nodes or spleens of DSG3-expressing skin-grafted Dsg3-/- mice into Rag1-/- mice. The TFH cells and CD4+ T cells in PBMCs from PV patients were examined by flow cytometry. RESULTS: Among CD4+ T cells from the mouse model, ICOS-positive TFH cells were associated with B-cell differentiation and were required for disease induction. Using an MHC class II tetramer, DSG3-specific ICOS+ TFH cells were found to be associated with anti-DSG3 antibody production and expanded in the absence of B cells. In human PV, the frequency of ICOS+CXCR5+PD-1+ memory CD4+ T cells correlated with the autoantibody level. Treatment with anti-ICOS blocking antibodies targeting ICOS+ TFH cells decreased the anti-DSG3 antibody level and delayed disease progression in vivo. CONCLUSIONS: Mouse Dsg3-specific ICOS+ TFH cells and human ICOS+CXCR5+PD-1+ TH cells are associated with the anti-DSG3 antibody response in PV. ICOS expressed on CXCR5+PD-1+ TH cells may be a therapeutic target for PV.


Assuntos
Anti-Inflamatórios/uso terapêutico , Anticorpos Bloqueadores/uso terapêutico , Desmogleína 3/metabolismo , Centro Germinativo/imunologia , Proteína Coestimuladora de Linfócitos T Induzíveis/metabolismo , Pênfigo/terapia , Células Th1/metabolismo , Animais , Autoanticorpos/metabolismo , Desmogleína 3/genética , Modelos Animais de Doenças , Progressão da Doença , Citometria de Fluxo , Humanos , Memória Imunológica , Proteína Coestimuladora de Linfócitos T Induzíveis/imunologia , Camundongos , Camundongos Knockout , Pênfigo/imunologia , Receptor de Morte Celular Programada 1/metabolismo , Receptores CXCR5/metabolismo , Células Th1/imunologia
11.
Small ; 16(47): e2004372, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33136316

RESUMO

Next-generation energy storage devices such as lithium-sulfur batteries (LSBs) face several challenges including fast charging and high-power delivery. Thus, it is necessary to improve the stability of the electrodes with efficient electrochemical system. In this work, a durable sulfur cathode even at high rates is enabled via lean binder content. The binder consists of a chitosan cross-linked with a carboxylated nitrile-butadiene rubber (XNBR), which exhibits high affinity toward lithium polysulfide along with robust mechanical properties because of the synergistic effect of the polar chitosan and the elastomeric XNBR. Despite using extremely small content of binder (3 wt%), the LSB shows a highly stable long-term cycling performance with capacity retention decay values of 0.026% and 0.029% after 500 cycles at 5 and 10 C. Moreover, the cell shows an outstanding specific capacity of 228 mAh g-1 at an ultrahigh charge-discharge rate of 20 C. This approach may significantly improve the design of the sulfur cathode and pave a facile way to fabricate commercially viable next-generation energy storage devices.

12.
Int J Med Microbiol ; 310(2): 151391, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32007342

RESUMO

Lactobacillus plantarum has been identified as a probiotic bacterium owing to its role in immune regulation and maintenance of intestinal permeability. Here, we investigated the anti-colitic effects and mechanism of L. plantarum CBT LP3 (LP3). This in vivo study was performed using dextran sodium sulfate (DSS) to induce colitis in mice. Mice were randomly divided into three groups: a control supplied with normal drinking water, a DSS-treated group followed by oral administration of vehicle, and a DSS-treated group gavaged with LP3 daily for 7 days following DSS administration. An analysis of macrophages and T cell subsets harvesting from peritonium cavity cells and splenocytes was performed using a flow cytometric assay. Gene expression and cytokine profiles were measured using quantitative reverse transcriptase polymerase chain reaction. The administration of LP3 significantly attenuated disease activity and histolopathology compared to control. LP3 had anti-inflammatory effects, with increased induction of regulatory T cells and type 2 helper T cells in splenocytes and restoration of goblet cells accompanied by suppression of proinflammatory cytokine expressions. These findings suggest that L. plantarum CBT LP3 can be used as a potent immunomodulator, which has significant implications for IBD treatment.


Assuntos
Colite/imunologia , Colite/terapia , Lactobacillus plantarum , Probióticos/uso terapêutico , Subpopulações de Linfócitos T/imunologia , Animais , Colite/induzido quimicamente , Citocinas/imunologia , Sulfato de Dextrana , Modelos Animais de Doenças , Fatores Imunológicos/uso terapêutico , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Linfócitos T Reguladores/imunologia , Células Th2/imunologia
13.
J Am Acad Dermatol ; 82(3): 575-585.e1, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29438767

RESUMO

BACKGROUND: Several European countries recently developed international diagnostic and management guidelines for pemphigus, which have been instrumental in the standardization of pemphigus management. OBJECTIVE: We now present results from a subsequent Delphi consensus to broaden the generalizability of the recommendations. METHODS: A preliminary survey, based on the European Dermatology Forum and the European Academy of Dermatology and Venereology guidelines, was sent to a panel of international experts to determine the level of consensus. The results were discussed at the International Bullous Diseases Consensus Group in March 2016 during the annual American Academy of Dermatology conference. Following the meeting, a second survey was sent to more experts to achieve greater international consensus. RESULTS: The 39 experts participated in the first round of the Delphi survey, and 54 experts from 21 countries completed the second round. The number of statements in the survey was reduced from 175 topics in Delphi I to 24 topics in Delphi II on the basis of Delphi results and meeting discussion. LIMITATIONS: Each recommendation represents the majority opinion and therefore may not reflect all possible treatment options available. CONCLUSIONS: We present here the recommendations resulting from this Delphi process. This international consensus includes intravenous CD20 inhibitors as a first-line therapy option for moderate-to-severe pemphigus.


Assuntos
Fatores Imunológicos/administração & dosagem , Pênfigo/diagnóstico , Pênfigo/terapia , Plasmaferese , Guias de Prática Clínica como Assunto , Academias e Institutos/normas , Administração Intravenosa , Antígenos CD20/imunologia , Terapia Combinada/métodos , Terapia Combinada/normas , Consenso , Técnica Delphi , Dermatologia/métodos , Dermatologia/normas , Quimioterapia Combinada/métodos , Quimioterapia Combinada/normas , Europa (Continente) , Glucocorticoides/administração & dosagem , Humanos , Pênfigo/imunologia , Rituximab/administração & dosagem , Índice de Gravidade de Doença
14.
J Gastroenterol Hepatol ; 34(1): 178-185, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29933526

RESUMO

BACKGROUND AND AIM: Nuclear factor kappa B (NF-κB) activation and endoplasmic reticulum (ER) stress signaling play significant roles in the pathogenesis of inflammatory bowel disease (IBD). Thus, we evaluated whether new therapeutic probiotics have anti-colitic effects, and we investigated their mechanisms related to NF-κB and ER-stress pathways. METHODS: Luciferase, nitric oxide, and cytokine assays using HT-29 or RAW264.7 cells were conducted. Mouse colitis was induced using dextran sulfate sodium and confirmed by disease activity index and histology. Macrophages and T-cell subsets in isolated peritoneal cavity cells and splenocytes were analyzed by flow cytometry. Gene and cytokine expression profiles were determined using reverse-transcription polymerase chain reaction. RESULTS: Lactobacillus acidophilus (LA1) and Pediococcus pentosaceus inhibited nitric oxide production in RAW264.7 cells, but only LA1 inhibited Tnfa and induced Il10 expression. LA1 increased the lifespan of dextran sulfate sodium-treated mice and attenuated the severity of colitis by inducing M2 macrophages in peritoneal cavity cells and Th2 and Treg cells in splenocytes. The restoration of goblet cells in the colon was accompanied by the induction of Il10 expression and the suppression of pro-inflammatory cytokines. Additionally, we found that LA1 exerts an anti-colitic effect by improving ER stress in HT-29 cells as well as in vivo. CONCLUSIONS: We showed that LA1 significantly interferes with ER stress and suppresses NF-κB activation. Our findings suggest that LA1 can be used as a potent immunomodulator in IBD treatment, and the regulation of ER stress may have significant implications in treating IBD.


Assuntos
Colite/imunologia , Colite/terapia , Estresse do Retículo Endoplasmático , Mucosa Intestinal/imunologia , Lactobacillus acidophilus , Probióticos/farmacologia , Animais , Colite/induzido quimicamente , Colite/patologia , Sulfato de Dextrana , Células Caliciformes , Células HT29 , Humanos , Interleucina-10/metabolismo , Mucosa Intestinal/patologia , Macrófagos , Masculino , Camundongos , NF-kappa B , Óxido Nítrico/antagonistas & inibidores , Pediococcus pentosaceus , Cavidade Peritoneal/citologia , Células RAW 264.7 , Linfócitos T Reguladores/imunologia , Células Th2/imunologia , Fator de Necrose Tumoral alfa/antagonistas & inibidores
15.
Exp Dermatol ; 27(11): 1304-1308, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-30092122

RESUMO

Atopic dermatitis (AD) is a chronic inflammatory skin disease whose prevalence is increasing worldwide. Filaggrin (FLG) is essential for the development of the skin barrier, and its genetic mutations are major predisposing factors for AD. In this study, we developed a convenient and practical method to detect FLG mutations in AD patients using peptide nucleic acid (PNA) probes labelled with fluorescent markers for rapid analysis. Fluorescence melting curve analysis (FMCA) precisely identified FLG mutations based on the distinct difference in the melting temperatures of the wild-type and mutant allele. Moreover, PNA probe-based FMCA easily and accurately verified patient samples with both heterozygote and homozygote FLG mutations, providing a high-throughput method to reliable screen AD patients. Our method provides a convenient, rapid and accurate diagnostic tool to identify potential AD patients allowing for early preventive treatment, leading to lower incidence rates of AD, and reducing total healthcare expenses.


Assuntos
Análise Mutacional de DNA/métodos , Sondas de DNA , Dermatite Atópica/diagnóstico , Dermatite Atópica/genética , Proteínas de Filamentos Intermediários/genética , Alelos , Estudos de Casos e Controles , Proteínas Filagrinas , Fluorescência , Heterozigoto , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Homozigoto , Humanos , Mutação , Ácidos Nucleicos Peptídicos/genética , Temperatura de Transição
17.
J Allergy Clin Immunol ; 137(5): 1466-1476.e3, 2016 05.
Artigo em Inglês | MEDLINE | ID: mdl-26824999

RESUMO

BACKGROUND: Psoriasis is one of the most common chronic inflammatory diseases of the skin. Recently, IL-17-producing T cells have been shown to play a critical role in psoriatic inflammation. Programmed cell death 1 (PD-1) is a coinhibitory receptor expressed on T cells in various chronic inflammatory diseases; however, the expression and function of PD-1 during psoriatic inflammation have not previously been characterized. OBJECTIVE: We examined PD-1 expression on IL-17A-producing T cells from imiquimod-treated mice and patients with psoriasis. Additionally, we investigated the therapeutic effect of recombinant programmed cell death ligand 1 (PD-L1) protein on imiquimod-induced psoriatic inflammation. METHODS: PD-1 expression on IL-17A-producing γδ T cells from imiquimod-treated mice was examined by means of multicolor flow cytometric analysis. In the psoriatic skin of patients, PD-1 and IL-17A expression was analyzed by using immunofluorescence. The therapeutic effect of PD-L1-Fc fusion protein (PD-L1-Fc) was assessed in imiquimod-treated mice ex vivo and in vivo. RESULTS: During imiquimod-induced psoriatic inflammation, PD-1 is overexpressed on CD27(-)Vγ1(-) γδ T cells. Furthermore, PD-1 expression on IL-17A(+) T cells was confirmed in psoriatic skin tissues from patients and imiquimod-treated mice. In the CD27(-)Vγ1(-) γδ T-cell population, Vγ4(-) γδ T cells with Vγ6 mRNA expression showed a high level of PD-1 expression. Furthermore, these PD-1(hi)Vγ4(-) (Vγ6(+)) γδ T cells were specialized for anti-CD3-induced IL-17A production, which was inhibited by PD-L1-Fc treatment. In imiquimod-treated mice PD-L1-Fc reduced psoriatic inflammation when given alone and enhanced the therapeutic effect of anti-p40 when given in combination. CONCLUSION: PD-1 is overexpressed in IL-17A-producing T cells in both imiquimod-treated mice and patients with psoriasis. Moreover, recombinant PD-L1-Fc alleviates psoriatic inflammation in imiquimod-treated mice.


Assuntos
Interleucina-17/metabolismo , Receptor de Morte Celular Programada 1/metabolismo , Psoríase/metabolismo , Subpopulações de Linfócitos T/metabolismo , Adjuvantes Imunológicos , Aminoquinolinas , Animais , Antígeno B7-H1/farmacologia , Antígeno B7-H1/uso terapêutico , Humanos , Imiquimode , Inflamação/metabolismo , Camundongos Endogâmicos C57BL , Psoríase/induzido quimicamente , Psoríase/tratamento farmacológico , Proteínas Recombinantes/farmacologia , Proteínas Recombinantes/uso terapêutico , Pele/efeitos dos fármacos , Pele/metabolismo
18.
Int J Mol Sci ; 18(10)2017 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-29019940

RESUMO

This study evaluated frequency-dependent effects of extremely low frequency electromagnetic fields (ELF-EMFs) on melanogenesis by melanocytes in vitro. Melanocytes were exposed to 2 mT EMFs at 30-75 Hz for 3 days before melanogenesis was examined. Exposure to ELF-EMFs at 50 and 60 Hz induced melanogenic maturation without cell damage, without changing cell proliferation and mitochondrial activity. Melanin content and tyrosinase activity of cells exposed to 50 Hz were higher than in controls, and mRNA expression of tyrosinase-related protein-2 was elevated relative to controls at 50 Hz. Phosphorylated cyclic adenosine monophosphate response element-binding protein (p-CREB) levels were higher than controls in cells exposed to ELF-EMFs at 50-75 Hz. Immunohistochemical staining showed that melanocyte-specific markers (HMB45, Melan-A) were strongly expressed in cells exposed to EMFs at 50 and 60 Hz compared to controls. Thus, exposure to ELF-EMFs at 50 Hz could stimulate melanogenesis in melanocytes, through activation of p-CREB and p-p38 and inhibition of phosphorylated extracellular signal-regulated protein kinase and phosphorylated stress-activated protein kinase/c-Jun N-terminal kinase. The results may form the basis of an appropriate anti-gray hair treatment or be applied in a therapeutic device for inducing repigmentation in the skin of vitiligo patients.


Assuntos
Proteína de Ligação a CREB/metabolismo , Campos Eletromagnéticos , Melaninas/biossíntese , Melanócitos/metabolismo , Fatores de Transcrição/metabolismo , Biomarcadores/metabolismo , Proteína de Ligação a CREB/genética , Linhagem Celular , Proliferação de Células , Humanos , Oxirredutases Intramoleculares/genética , Oxirredutases Intramoleculares/metabolismo , Sistema de Sinalização das MAP Quinases , Mitocôndrias/metabolismo , Monofenol Mono-Oxigenase/metabolismo , Fosforilação , Fatores de Transcrição/genética , Vitiligo/terapia
19.
Acta Derm Venereol ; 96(4): 535-9, 2016 May.
Artigo em Inglês | MEDLINE | ID: mdl-26560051

RESUMO

The epidemiological and clinicopathological features of cutaneous lymphoma may vary by geographical area. However, only a few large-scale epidemiological studies of cutaneous lymphoma have been performed, mainly in the USA and Europe. This aim of this study was to determine the recent characteristics of cutaneous lymphoma in Korea according to the WHO/EORTC classification. A total of 422 patients with newly diagnosed cutaneous lymphoma from January 2009 to December 2013 comprising 293 cases of mature T-cell and natural killer (NK)-cell lymphoma and 39 cases of mature B-cell lymphoma were retrospectively reviewed. The incidence of mature B-cell lymphoma was lower in Korea than in Europe and the USA. Diffuse large B-cell lymphoma was more prevalent in Korea than in Western countries. The incidence of extranodal NK/T-cell lymphoma, nasal-type was higher in Korea than in Western countries and Japan.


Assuntos
Linfoma de Células B/epidemiologia , Linfoma Extranodal de Células T-NK/epidemiologia , Linfoma Cutâneo de Células T/epidemiologia , Neoplasias Cutâneas/epidemiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Feminino , Humanos , Incidência , Linfoma de Células B/diagnóstico , Linfoma de Células B/terapia , Linfoma Extranodal de Células T-NK/diagnóstico , Linfoma Extranodal de Células T-NK/terapia , Linfoma Difuso de Grandes Células B/epidemiologia , Linfoma Cutâneo de Células T/diagnóstico , Linfoma Cutâneo de Células T/terapia , Masculino , Pessoa de Meia-Idade , Prevalência , Prognóstico , República da Coreia/epidemiologia , Estudos Retrospectivos , Neoplasias Cutâneas/diagnóstico , Neoplasias Cutâneas/terapia , Fatores de Tempo , Adulto Jovem
20.
Bioelectromagnetics ; 37(3): 163-174, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26991921

RESUMO

The purpose of this study was to investigate the effect of Schwann-like cells combined with pulsed electromagnetic field (PEMF) on peripheral nerve regeneration. Schwann-like cells were derived from human dental pulp stem cells (hDPSCs) and verified with CD104, S100, glial fibrillary acidic protein (GFAP), laminin, and P75NTR immunocytochemistry. Gene expression of P75NTR and S100 were analyzed. Male Sprague-Dawley rats (200-250g, 6-week-old) were divided into seven groups (n = 10 each): control, sham, PEMF, hDPSCs, hDPSCs + PEMF, Schwann-like cells, Schwann-like cells + PEMF. Cells were transplanted (1 × 106 /10µl/rat) at crush-injury site or combined with PEMF (50 Hz, 1 h/day, 1 mT). Nerve regeneration was evaluated with functional test, histomorphometry and retrograde labelled neurons. Schwann-like cells expressed CD104, S100, GFAP, laminin, and p75 neurotrophin receptor (P75NTR ). P75NTR and S100 mRNA expression was highest in Schwann-like cells + PEMF group, which also showed increased Difference and Gap scores. Axons and retrograde labeled neurons increased in all treatment groups. Schwann-like cells, hDPSCs with or without PEMF, and PEMF only improved peripheral nerve regeneration. Schwann-like cells + PEMF showed highest regeneration ability; PEMF has additive effect on hDPSCs, Schwann-like cell in vitro and nerve regeneration ability after transplantation in vivo. Bioelectromagnetics. 37:163-174, 2016. © 2016 Wiley Periodicals, Inc.

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