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1.
Science ; 260(5109): 789-92, 1993 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-17746112

RESUMO

The fabrication of improved ceramic-matrix composites will require a better understanding of processing variables and how they control the development of the composite microstructure. Noninvasive, high-resolution methods of x-ray tomography have been used to measure the growth of silicon carbide in a woven Nicalon-fiber composite during chemical vapor infiltration. The high spatial resolution allows one to measure the densification within individual fiber tows and to follow the closure of macroscopic pores in situ. The experiments provide a direct test of a recently proposed model that describes how the surface area available for matrix deposition changes during infiltration. The measurements indicate that this surface area is independent of the fiber architecture and location within the preform and is dominated by large-scale macroporosity during the final stages of composite consolidation. The measured surface areas are in good agreement with the theoretical model.

2.
Caries Res ; 43(1): 70-7, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-19208991

RESUMO

The objective of this article is to critically evaluate the methods that are used to assess outcomes of remineralization of dentin. Currently, the most used assessment methods fall either into quantitative analysis of the mineral content of the remineralized structures or dry measurements of their mechanical properties. Properties obtained from the dehydrated organic dentin matrix may not reflect the true mechanical behavior of the remineralized tissue under physiological and hydrated conditions. Here we seek to clarify the biomechanical aspects of remineralization of dentin, pointing out the effects of hydration and dehydration on the mechanical properties of treated tissues. We also emphasize that a more appropriate endpoint to evaluate the effectiveness of remineralization in dentin should be associated with the recovery of the mechanical properties of the hydrated tissue, which is presumed to correlate well with its overall functionality.


Assuntos
Análise do Estresse Dentário/métodos , Dentina/química , Dentina/fisiologia , Remineralização Dentária , Fenômenos Biomecânicos , Dessecação , Elasticidade , Colágenos Fibrilares/química , Testes de Dureza , Humanos , Microrradiografia , Microscopia Eletrônica de Transmissão , Modelos Químicos , Avaliação de Resultados em Cuidados de Saúde/métodos , Análise Espectral , Termogravimetria , Água
3.
J Struct Biol ; 162(3): 404-10, 2008 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-18467127

RESUMO

Dentin and bone derive their mechanical properties from a complex arrangement of collagen type-I fibrils reinforced with nanocrystalline apatite mineral in extra- and intrafibrillar compartments. While mechanical properties have been determined for the bulk of the mineralized tissue, information on the mechanics of the individual fibril is limited. Here, atomic force microscopy was used on individual collagen fibrils to study structural and mechanical changes during acid etching. The characteristic 67 nm periodicity of gap zones was not observed on the mineralized fibril, but became apparent and increasingly pronounced with continuous demineralization. AFM-nanoindentation showed a decrease in modulus from 1.5 GPa to 50 MPa during acid etching of individual collagen fibrils and revealed that the modulus profile followed the axial periodicity. The nanomechanical data, Raman spectroscopy and SAXS support the hypothesis that intrafibrillar mineral etches at a substantially slower rate than the extrafibrillar mineral. These findings are relevant for understanding the biomechanics and design principles of calcified tissues derived from collagen matrices.


Assuntos
Colágeno/química , Dente/química , Apatitas/química , Fenômenos Biomecânicos , Dentina/química , Dureza , Humanos , Microscopia de Força Atômica/métodos , Nanopartículas/química , Espalhamento de Radiação , Análise Espectral Raman , Estresse Mecânico , Propriedades de Superfície , Dente/patologia , Raios X
4.
J Dent Res ; 85(11): 1022-6, 2006 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-17062743

RESUMO

Healthy dentin, the mineralized tissue that makes up the bulk of the tooth, is naturally hydrated in vivo; however, it is known that various chemical reagents, including acetone and ethanol, can induce dehydration and thereby affect its properties. Here, we sought to investigate this in light of the effect of alcohol on the mechanical properties of dentin, specifically by measuring the stiffness, strength, and toughness of dentin in simulated body fluid and Scotch whisky. Results indicated that chemical dehydration induced by the whisky had a significant beneficial effect on the elastic modulus, strength, and fracture toughness of dentin. Although this made teeth more resistant to fracture, the change in properties was fully reversible upon rehydration. This effect is considered to be associated with increased cross-linking of the collagen molecules from intermolecular hydrogen-bonding, where water is replaced with weaker hydrogen-bond-forming solvents such as alcohol.


Assuntos
Dentina/química , Dentina/efeitos dos fármacos , Etanol/farmacologia , Fraturas dos Dentes/prevenção & controle , Acetona/farmacologia , Bebidas Alcoólicas , Animais , Colágeno/química , Análise do Estresse Dentário , Dessecação , Elasticidade , Elefantes , Ligação de Hidrogênio , Masculino , Metanol/farmacologia , Maleabilidade , Resistência à Tração
5.
Biomaterials ; 26(10): 1195-204, 2005 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-15451639

RESUMO

Although the propagation of fatigue cracks has been recognized as a problem of clinical significance in dentin, there have been few fracture mechanics-based studies that have investigated this issue. In the present study, in vitro cyclic fatigue experiments were conducted over a range of cyclic frequencies (1-50 Hz) on elephant dentin in order to quantify fatigue-crack growth behavior from the perspective of understanding the mechanism of fatigue in dentin. Specifically, results obtained for crack extension rates along a direction parallel to the dentinal tubules were found to be well described by the stress-intensity range, DeltaK, using a simple Paris power-law approach with exponents ranging from 12 to 32. Furthermore, a frequency dependence was observed for the crack-growth rates, with higher growth rates associated with lower frequencies. By using crack-growth experiments involving alternate cyclic and static loading, such fatigue-crack propagation was mechanistically determined to be the result of a "true" cyclic fatigue mechanism, and not simply a succession of static fracture events. Furthermore, based on the observed frequency dependence of fatigue-crack growth in dentin and observations of time-dependent crack blunting, a cyclic fatigue mechanism involving crack-tip blunting and re-sharpening is proposed. These results are deemed to be of importance for an improved understanding of fatigue-related failures in teeth.


Assuntos
Dentina/química , Dentina/fisiopatologia , Fraturas de Estresse/fisiopatologia , Fraturas dos Dentes/fisiopatologia , Animais , Fenômenos Biomecânicos/métodos , Dentina/patologia , Elasticidade , Elefantes , Fraturas de Estresse/patologia , Dureza , Testes de Dureza , Estresse Mecânico , Fraturas dos Dentes/patologia
6.
Biomaterials ; 26(2): 217-31, 2005 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-15207469

RESUMO

An understanding of the evolution of toughness is essential for the mechanistic interpretation of the fracture of cortical bone. In the present study, in vitro fracture experiments were conducted on human cortical bone in order to identify and quantitatively assess the salient toughening mechanisms. The fracture toughness was found to rise linearly with crack extension (i.e., rising resistance- or R-curve behavior) with a mean crack-initiation toughness, K0 of approximately 2 MPa square root m for crack growth in the proximal-distal direction. Uncracked ligament bridging, which was observed in the wake of the crack, was identified as the dominant toughening mechanism responsible for the observed R-curve behavior. The extent and nature of the bridging zone was examined quantitatively using multi-cutting compliance experiments in order to assess the bridging zone length and estimate the bridging stress distribution. Additionally, time-dependent cracking behavior was observed at stress intensities well below those required for overload fracture; specifically, slow crack growth occurred at growth rates of approximately 2 x 10(-9) m/s at stress intensities approximately 35% below the crack-initiation toughness. In an attempt to measure slower growth rates, it was found that the behavior switched to a regime dominated by time-dependent crack blunting, similar to that reported for dentin; however, such blunting was apparent over much slower time scales in bone, which permitted subcritical crack growth to readily take place at higher stress intensities.


Assuntos
Fraturas do Úmero/patologia , Fraturas do Úmero/fisiopatologia , Modelos Biológicos , Adulto , Fenômenos Biomecânicos/métodos , Cadáver , Força Compressiva , Simulação por Computador , Elasticidade , Dureza , Testes de Dureza , Humanos , Fraturas do Úmero/diagnóstico por imagem , Técnicas In Vitro , Radiografia , Estresse Mecânico , Viscosidade , Suporte de Carga
7.
Biomaterials ; 26(16): 3363-76, 2005 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-15603832

RESUMO

Many fractures occur in teeth that have been altered, for example restored or endodontically repaired. It is therefore essential to evaluate the structure and mechanical properties of these altered dentins. One such altered form of dentin is transparent (sometimes called sclerotic) dentin, which forms gradually with aging. The present study focuses on differences in the structure and mechanical properties of normal versus transparent dentin. The mineral concentration, as measured by X-ray computed microtomography, was significantly higher in transparent dentin, the elevated concentration being consistent with the closure of the tubule lumens. Crystallite size, as measured by small angle X-ray scattering, was slightly smaller in transparent dentin, although the importance of this finding requires further study. The elastic properties were unchanged by transparency; however, transparent dentin, unlike normal dentin, exhibited almost no yielding before failure. In addition, the fracture toughness was lowered by roughly 20% while the fatigue lifetime was deleteriously affected at high stress levels. These results are discussed in terms of the altered microstructure of transparent dentin.


Assuntos
Envelhecimento , Dentina/química , Raiz Dentária/metabolismo , Idoso , Solubilidade da Dentina , Dureza , Humanos , Minerais/metabolismo , Espalhamento de Radiação , Estresse Mecânico , Síncrotrons , Resistência à Tração , Fatores de Tempo , Tomografia Computadorizada por Raios X/métodos , Desmineralização do Dente , Raios X
8.
Biomaterials ; 26(14): 2183-95, 2005 May.
Artigo em Inglês | MEDLINE | ID: mdl-15576194

RESUMO

Although fatigue damage in bone induced by cyclic loading has been recognized as a problem of clinical significance, few fracture mechanics based studies have investigated how incipient cracks grow by fatigue in this material. In the present study, in vitro cyclic fatigue experiments were performed in order to quantify fatigue-crack growth behavior in human cortical bone. Crack-growth rates spanning five orders of magnitude were obtained for the extension of macroscopic cracks in the proximal-distal direction; growth-rate data could be well characterized by the linear-elastic stress-intensity range, using a simple (Paris) power law with exponents ranging from 4.4 to 9.5. Mechanistically, to discern whether such behavior results from "true" cyclic fatigue damage or is simply associated with a succession of quasi-static fracture events, cyclic crack-growth rates were compared to those measured under sustained (non-cyclic) loading. Measured fatigue-crack growth rates were found to exceed those "predicted" from the sustained load data at low growth rates ( approximately 3 x 10(-10) to 5 x 10(-7) m/cycle), suggesting that a "true" cyclic fatigue mechanism, such as alternating blunting and re-sharpening of the crack tip, is active in bone. Conversely, at higher growth rates ( approximately 5 x 10(-7) to 3 x 10(-5) m/cycle), the crack-growth data under sustained loads integrated over the loading cycle reasonably predicts the cyclic fatigue data, indicating that quasi-static fracture mechanisms predominate. The results are discussed in light of the occurrence of fatigue-related stress fractures in cortical bone.


Assuntos
Fraturas de Estresse/fisiopatologia , Úmero/fisiopatologia , Modelos Biológicos , Adulto , Cadáver , Força Compressiva , Simulação por Computador , Fraturas de Estresse/etiologia , Fraturas de Estresse/patologia , Humanos , Úmero/ultraestrutura , Técnicas In Vitro , Periodicidade , Estresse Mecânico , Fatores de Tempo
9.
J Biomech ; 38(7): 1517-25, 2005 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-15922763

RESUMO

Micromechanical models for fracture initiation that incorporate local failure criteria have been widely developed for metallic and ceramic materials; however, few such micromechanical models have been developed for the fracture of bone. In fact, although the fracture event in "hard" mineralized tissues such as bone is commonly believed to be locally strain-controlled, only recently has there been experimental evidence (using double-notched four-point bend testing) to support this widely held belief. In the present study, we seek to shed further light on the nature of the local cracking events that precede catastrophic fracture in human cortical bone, and to define their relationship to the microstructure. Specifically, numerical computations are reported that demonstrate that the stress and strain states ahead of such a notch are qualitatively similar irrespective of the deformation mechanism (pressure-insensitive plasticity vs. pressure-sensitive microcracking). Furthermore, we use the double-notched test to examine crack-microstructure interactions from a perspective of determining the salient toughening mechanisms in bone and to characterize how these may affect the anisotropy in fracture properties. Based on preliminary micromechanical models of these processes, the relative contributions of various toughening mechanisms are established. In particular, crack deflection and uncracked-ligament bridging are identified as the major mechanisms of toughening in cortical bone.


Assuntos
Fraturas do Úmero/fisiopatologia , Úmero/lesões , Úmero/fisiopatologia , Modelos Biológicos , Adulto , Anisotropia , Cadáver , Força Compressiva , Simulação por Computador , Elasticidade , Feminino , Dureza , Testes de Dureza/métodos , Humanos , Técnicas In Vitro , Estresse Mecânico , Resistência à Tração
10.
Acta Biomater ; 1(1): 31-43, 2005 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-16701778

RESUMO

Although healthy dentin is invariably hydrated in vivo, from a perspective of examining the mechanisms of fracture in dentin, it is interesting to consider the role of water hydration. Furthermore, it is feasible that exposure to certain polar solvents, e.g., those found in clinical adhesives, can induce dehydration. In the present study, in vitro deformation and fracture experiments, the latter involving a resistance-curve (R-curve) approach (i.e., toughness evolution with crack extension), were conducted in order to assess changes in the constitutive and fracture behavior induced by three common solvents-acetone, ethanol and methanol. In addition, nanoindentation-based experiments were performed to evaluate the deformation behavior at the level of individual collagen fibers and ultraviolet Raman spectroscopy to evaluate changes in bonding. The results indicate a reversible effect of chemical dehydration, with increased fracture resistance, strength, and stiffness associated with lower hydrogen bonding ability of the solvent. These results are analyzed both in terms of intrinsic and extrinsic toughening phenomena to further understand the micromechanisms of fracture in dentin and the specific role of water hydration.


Assuntos
Dentina/química , Dentina/fisiologia , Animais , Fenômenos Biomecânicos , Colágeno/química , Dessecação , Elefantes , Ligação de Hidrogênio , Técnicas In Vitro , Masculino , Teste de Materiais , Microscopia de Força Atômica , Nanotecnologia , Solventes , Análise Espectral Raman , Fraturas dos Dentes/etiologia , Fraturas dos Dentes/fisiopatologia , Água
11.
J Bone Miner Res ; 13(5): 839-45, 1998 May.
Artigo em Inglês | MEDLINE | ID: mdl-9610748

RESUMO

A finite-element model was used to explore the relationship between connectivity density and the elastic modulus of trabecular bone. Six cubic specimens of trabecular bone, three prepared from human distal radii and three from L1 vertebrae, were imaged with synchrotron microtomography. The three-dimensional images were reconstructed into binary volumes of mineralized bone and soft tissue, and incorporated into the finite-element model. The relationship between three-dimensional connectivity and elastic modulus was explored by uniform thinning (atrophy) and thickening (recovery) of the trabecular bone. Though no functional relationship was found between connectivity and elastic modulus, there was a linear relationship, after a full cycle of atrophy and recovery, between the loss of elastic modulus and the overall loss of connectivity. The results indicate that recovery of mechanical function depends on preserving or restoring trabecular connectivity.


Assuntos
Osso e Ossos/anatomia & histologia , Osso e Ossos/fisiologia , Idoso , Idoso de 80 Anos ou mais , Fenômenos Biomecânicos , Remodelação Óssea/fisiologia , Elasticidade , Fraturas Ósseas/etiologia , Humanos , Processamento de Imagem Assistida por Computador , Vértebras Lombares/anatomia & histologia , Vértebras Lombares/fisiologia , Masculino , Pessoa de Meia-Idade , Modelos Anatômicos , Osteoporose/complicações , Osteoporose/patologia , Osteoporose/fisiopatologia , Rádio (Anatomia)/anatomia & histologia , Rádio (Anatomia)/fisiologia , Fatores de Risco , Tomografia Computadorizada por Raios X
12.
J Bone Miner Res ; 10(2): 264-70, 1995 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-7754806

RESUMO

Osteoporosis, which is correlated with low bone mass and increased bone fragility, is responsible for about 1.2 million fractures per year in the United States. We have used the three-dimensional (3-D), X-ray tomographic microscope to image the trabecular bone architecture of the proximal tibias of six Sprague-Dawley rats in vivo. Three of these rats were then ovariectomized to induce estrogen depletion, and three remained as controls. Five weeks later, the tibias were reimaged. The ovariectomized rats lost approximately 65% of their trabecular bone volume as compared with an insignificant change in the control rats. The connectivity density of the trabecular bone, as measured by the Euler characteristic, was linearly correlated with trabecular bone volume, even in the ovariectomized rats. Hypoestrogenemic bone loss manifested itself in greatly reduced connectivity and fewer trabecular elements, but not in thinning of trabeculae. The ability to microscopically image sequential changes in the 3-D architecture of trabecular bone in vivo will allow exploration of the earliest stages of hypoestrogenemic bone loss and to more rapidly test the effectiveness of new clinical treatments for this major public health problem.


Assuntos
Densidade Óssea/fisiologia , Osteoporose Pós-Menopausa/diagnóstico por imagem , Tíbia/diagnóstico por imagem , Tomografia Computadorizada por Raios X/métodos , Animais , Modelos Animais de Doenças , Feminino , Fraturas Ósseas/epidemiologia , Humanos , Osteoporose Pós-Menopausa/epidemiologia , Osteoporose Pós-Menopausa/fisiopatologia , Ovariectomia , Ratos , Ratos Sprague-Dawley , Medição de Risco , Tíbia/fisiologia
13.
J Bone Miner Res ; 13(2): 229-36, 1998 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-9495515

RESUMO

Estrogen deficiency results in a loss of trabecular bone mass and structure that leads to an increased incidence of osteoporotic fractures. The purpose of this study was to determine the time course for trabecular structure deterioration and changes in bone turnover just after ovariectomy in the rat. Six-month-old female virgin Sprague-Dawley rats had their right proximal tibia scanned by X-ray tomographic microscopy (XTM) at baseline (day 0). Animals were then randomized into two groups, and in each group 9 were sham-operated and 11 were ovariectomized and had repeat XTM scans on days 5, 13, 29, and 42 postovariectomy in group 1 and on days 8, 13, 33, and 50 postovariectomy in group 2. Urine was collected for deoxypyridinoline (DPD) cross-link measurements 24 h before each XTM scan and analyzed by ELISA. Trabecular bone structural variables and bone turnover endpoints were calculated from XTM data and standard histomorphometry. Trabecular connectivity decreased 27% by days 5 and 8 postovariectomy (p < 0.01) and continued to decrease up to day 50 postovariectomy (p < 0.01). The trabecular bone volume decreased 25% by 8 days postovariectomy (p < 0.01), and it continued to decrease through day 50. DPD cross-link excretion had increased 37% on day 13 (p < 0.01) and by over 100% of baseline by day 50 postovariectomy. Trabecular bone connectivity and volume deteriorate rapidly while DPD cross-link excretion increased more slowly in acute estrogen deficiency. These data suggest that if an agent is to preserve fully trabecular bone structure, it must be instituted very early in the estrogen-deficient state. They also suggest that a lag time exists before DPD excretion properly mirrors newly induced conditions of high bone turnover in this rat model.


Assuntos
Reabsorção Óssea/metabolismo , Osteoclastos/metabolismo , Aminoácidos/urina , Animais , Biomarcadores/urina , Reabsorção Óssea/diagnóstico por imagem , Reabsorção Óssea/urina , Estrogênios/deficiência , Feminino , Membro Posterior/diagnóstico por imagem , Membro Posterior/metabolismo , Humanos , Ovariectomia , Ratos , Ratos Sprague-Dawley , Tomografia Computadorizada por Raios X
14.
J Bone Miner Res ; 10(10): 1470-7, 1995 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-8686502

RESUMO

Previous studies have determined that intermittent parathyroid hormone (PTH) therapy increases bone mass and improves biomechanical strength in osteopenic animal models. The purpose of this investigation was to determine if intermittent human parathyroid hormone (hPTH[1-34]) therapy increased trabecular bone volume and connectivity in a rat model of established osteopenia using three-dimensional (3D) ex vivo in situ morphometry by X-ray tomographic methods (XTM). Six-month-old retired Sprague-Dawley breeder rats were used. Thirty animals were ovariectomized (OVX) and six were Sham operated. On day 56, post-OVX, a prePTH-treatment OVX groups was sacrificed. The remaining OVX animals were randomized into four groups of six animals each, given injections 5 out of every 7 days for 28 days of either vehicle or hPTH(1-34) at 4, 40, or 400 microg/kg of body weight (BW)/day and were sacrificed on day 84 post-OVX. At sacrifice, the left proximal tibias were harvested for XTM scans. hPTH(1-34) at medium and high doses significantly increased trabecular bone volume and trabecular thickness compared with ovariectomized animals treated with vehicle (p<0.05). The trabecular bone volume was equal to or greater than the Sham-operated animals in both hPTH(1-34) 40 and 400 microg/kg of BW treatment groups. Trabecular bone connectivity decreased by nearly 50% compared to the S ham-operated group at day 84 post-OVX and did not increase with any of the hPTH(1-34) treatments. Intermittent hPTH(1-34) treatment is osteopenic OVX rats increased trabecular bone volume to control levels or higher by thickening existing trabeculae. Human PTH(1-34) did not re-establish connectivity when therapy was started after 50% of the trabecular connectivity was lost. We hypothesize that to re-establish trabecular connectivity, a therapeutic intervention would have to be given before a significant distance between trabeculae has developed. Further studies will need to be done to refute or confirm our hypothesis.


Assuntos
Densidade Óssea/efeitos dos fármacos , Doenças Ósseas Metabólicas/tratamento farmacológico , Hormônio Paratireóideo/uso terapêutico , Fragmentos de Peptídeos/uso terapêutico , Animais , Doenças Ósseas Metabólicas/fisiopatologia , Modelos Animais de Doenças , Relação Dose-Resposta a Droga , Esquema de Medicação , Feminino , Humanos , Variações Dependentes do Observador , Hormônio Paratireóideo/administração & dosagem , Fragmentos de Peptídeos/administração & dosagem , Ratos , Ratos Sprague-Dawley , Reprodutibilidade dos Testes , Teriparatida , Tomografia por Raios X
15.
J Bone Miner Res ; 14(2): 206-14, 1999 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-9933474

RESUMO

To evaluate the ability of estrogen replacement therapy (ERT) to prevent changes in trabecular bone volume (BV/TV) and connectivity beginning either at ovariectomy (OVX) or 5-13 days after OVX in adult female rats, the right proximal tibial was examined by three-dimensional X-ray tomographic microscopy (XTM) in vivo. Animals had XTM scans of the right tibia and then were randomized into six groups (n = 9). Groups 2-6 had bilateral (OVX), while group 1 was sham-ovariectomized (OVXd) on day 0. Animals were treated with vehicle (groups 1 and 2) or 17beta-estradiol therapy (ERT) at 10 microg/kg three times per week starting at days 0, 5, 8, and 13 post-OVX (groups 3, 4, 5, and 6), until day 50 when they were rescanned by XTM and sacrificed. Trabecular bone structural variables were calculated from XTM data (BV/TVx and beta1/BV/TVx) and standard histomorphometry. Trabecular bone volume (BV/TVx) and the trabecular connections per cubic millimeter of trabecular bone (beta1/BV/TVx) were maintained in both sham-OVXd animals and OVX animals given ERT from the time of OVX. However, OVX + vehicle-treated animals lost 54% BV/TVx and 46% beta1/BV/TVx (p < 0. 01 from day 0). BV/TVx and beta1/BV/TVx decreased rapidly post-OVX to -22% and -25% at day 13 (p < 0.01 from day 0). ERT initiated at day 5, 8, and 13 post-OVX restored BV/TVx to baseline values at day 50 by modestly increasing trabecular plate thickness; however, beta1/BV/TVx was reduced in all OVX groups when compared with their baseline values. ERT also caused a significant reduction in bone turnover compared with OVX + vehicle; however, resorption was suppressed more than formation. These results demonstrate that ERT can restore the lost trabecular bone, but not trabecular connectivity, that occurs soon after OVX by allowing bone formation to continue in previously activated bone remodeling units while suppressing the production of new remodeling units. This may be the mechanism by which prompt intervention with estrogen and other antiresorptive agents can restore bone mass that has been lost from the increase in remodeling space, and thereby reduce the risk of osteoporotic fractures in postmenopausal women.


Assuntos
Osso e Ossos/efeitos dos fármacos , Osso e Ossos/patologia , Terapia de Reposição de Estrogênios , Animais , Densidade Óssea/efeitos dos fármacos , Remodelação Óssea/efeitos dos fármacos , Osso e Ossos/metabolismo , Modelos Animais de Doenças , Feminino , Humanos , Osteoporose Pós-Menopausa/tratamento farmacológico , Osteoporose Pós-Menopausa/metabolismo , Osteoporose Pós-Menopausa/patologia , Ovariectomia , Ratos , Ratos Sprague-Dawley , Fatores de Tempo , Tomografia por Raios X
16.
J Bone Miner Res ; 15(10): 1981-91, 2000 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-11028451

RESUMO

This article summarizes the results of a three-dimensional study of changes in the morphology of the L6 rat vertebra at 120 days after ovariectomy (OVX), with estrogen replacement therapy used as a positive control. Synchrotron radiation microtomography was used to quantify the structural parameters defining trabecular bone architecture, while finite-element methods were used to explore the relationships between these parameters and the compressive elastic behavior of the vertebrae. There was a 22% decrease in trabecular bone volume (TBV) and a 19% decline in mean trabecular thickness (Tb.Th) with OVX. This was accompanied by a 150% increase in trabecular connectivity, a result of the perforation of trabecular plates. Finite-element analysis of the trabecular bone removed from the cortical shell showed a 37% decline in the Young's modulus in compression after OVX with no appreciable change in the estrogen-treated group. The intact vertebrae (containing its trabecular bone) exhibited a 15% decrease in modulus with OVX, but this decline lacked statistical significance. OVX-induced changes in the trabecular architecture were different from those that have been observed in the proximal tibia. This difference was a consequence of the much more platelike structure of the trabecular bone in the vertebra.


Assuntos
Modelos Animais de Doenças , Vértebras Lombares/ultraestrutura , Osteoporose/patologia , Ovariectomia , Animais , Elasticidade , Terapia de Reposição de Estrogênios , Feminino , Imageamento Tridimensional , Microscopia de Força Atômica , Ratos , Ratos Sprague-Dawley , Estresse Mecânico , Síncrotrons , Tomografia
17.
Bone ; 33(4): 494-504, 2003 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-14555252

RESUMO

The finite element method, which has been successfully applied to studies of the elastic properties of trabecular bone, is now being used to simulate its failure. These simulations have used a geometrically linear (linear kinematic) approximation to the total stiffness matrix; nonlinear terms in the total stiffness matrix have been excluded from the computation in order to achieve efficiency. Because trabecular bone appears to be a slender (i.e., geometrically nonlinear) structure, we studied the validity of the linear kinematic approximation for simulating its failure. Two cases, designed to bracket the extremes of stability behavior, were explored: a single representative spicule of trabecular bone (case 1) and a volume of trabecular bone consisting of relatively low aspect ratio members (case 2). For case 1, geometrically linear (GL) and nonlinear (GNL) analyses were performed with two different materials models: a plastic damage model and a brittle damage model. When GNL terms were included in the total stiffness matrix, we found that load-path bifurcation preceded tissue failure regardless of the form of the damage model. This bifurcation was the result of a complex coupling between material yield and structural instability. The nature of this coupling was highly sensitive to the form of the damage model. None of these behaviors was observed in the linear analyses, where failure was insensitive to the form of the damage model and where structural instabilities were prevented from occurring. For case 2, compressive loading of a volume of trabecular bone, geometric nonlinear effects were pronounced. There was a bifurcation in load response that resulted in large apparent strain to failure. The GL simulations, on the other hand, precluded this bifurcation. We hypothesize that trabecular bone is a geometric nonlinear structure; nonlinear terms must be included in the total stiffness matrix to accurately simulate its failure.


Assuntos
Fraturas Ósseas/etiologia , Modelos Biológicos , Fenômenos Biomecânicos , Força Compressiva , Elasticidade , Fraturas Ósseas/diagnóstico por imagem , Fraturas Ósseas/patologia , Fraturas Ósseas/fisiopatologia , Humanos , Processamento de Imagem Assistida por Computador , Dinâmica não Linear , Síncrotrons , Resistência à Tração , Tomografia Computadorizada por Raios X/métodos
18.
Bone ; 35(6): 1240-6, 2004 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-15589205

RESUMO

Age-related deterioration of the fracture properties of bone, coupled with increased life expectancy, is responsible for increasing incidence of bone fracture in the elderly, and hence, an understanding of how its fracture properties degrade with age is essential. The present study describes ex vivo fracture experiments to quantitatively assess the effect of aging on the fracture toughness properties of human cortical bone in the longitudinal direction. Because cortical bone exhibits rising crack-growth resistance with crack extension, unlike most previous studies, the toughness is evaluated in terms of resistance-curve (R-curve) behavior, measured for bone taken from wide range of age groups (34-99 years). Using this approach, both the ex vivo crack-initiation and crack-growth toughness are determined and are found to deteriorate with age; the initiation toughness decreases some 40% over 6 decades from 40 to 100 years, while the growth toughness is effectively eliminated over the same age range. The reduction in crack-growth toughness is considered to be associated primarily with a degradation in the degree of extrinsic toughening, in particular, involving crack bridging in the wake of the crack.


Assuntos
Envelhecimento/fisiologia , Densidade Óssea/fisiologia , Fraturas de Estresse/fisiopatologia , Fraturas do Úmero/fisiopatologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Fraturas de Estresse/etiologia , Humanos , Fraturas do Úmero/etiologia , Úmero/fisiologia , Masculino , Pessoa de Meia-Idade
19.
Biomaterials ; 24(22): 3955-68, 2003 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-12834591

RESUMO

Toughening mechanisms based on the presence of collagen fibrils have long been proposed for mineralized biological tissues like bone and dentin; however, no direct evidence for their precise role has ever been provided. Furthermore, although the anisotropy of mechanical properties of dentin with respect to orientation has been suggested in the literature, accurate measurements to support the effect of orientation on the fracture toughness of dentin are not available. To address these issues, the in vitro fracture toughness of dentin, extracted from elephant tusk, has been characterized using fatigue-precracked compact-tension specimens tested in Hank's balanced salt solution at ambient temperature, with fracture paths perpendicular and parallel to the tubule orientations (and orientations in between) specifically being evaluated. It was found that the fracture toughness was lower where cracking occurred in the plane of the collagen fibers, as compared to crack paths perpendicular to the fibers. The origins of this effect on the toughness of dentin are discussed primarily in terms of the salient toughening mechanisms active in this material; specifically, the role of crack bridging, both from uncracked ligaments and by individual collagen fibrils, is considered. Estimates for the contributions from each of these mechanisms are provided from theoretical models available in the literature.


Assuntos
Materiais Biocompatíveis , Dentina/química , Dente/química , Animais , Anisotropia , Colágeno/química , Elefantes , Masculino , Microscopia Eletrônica de Varredura , Estresse Mecânico , Temperatura , Resistência à Tração
20.
Biomaterials ; 24(28): 5209-21, 2003 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-14568438

RESUMO

Few studies have focused on a description of the fracture toughness properties of dentin in terms of resistance-curve (R-curve) behavior, i.e., fracture resistance increasing with crack extension, particularly in light of the relevant toughening mechanisms involved. Accordingly, in the present study, fracture mechanics based experiments were conducted on elephant dentin in order to determine such R-curves, to identify the salient toughening mechanisms and to discern how hydration may affect their potency. Crack bridging by uncracked ligaments, observed directly by microscopy and X-ray tomography, was identified as a major toughening mechanism, with further experimental evidence provided by compliance-based experiments. In addition, with hydration, dentin was observed to display significant crack blunting leading to a higher overall fracture resistance than in the dehydrated material. The results of this work are deemed to be of importance from the perspective of modeling the fracture behavior of dentin and in predicting its failure in vivo.


Assuntos
Análise do Estresse Dentário/métodos , Dentina/lesões , Dentina/fisiopatologia , Modelos Biológicos , Fraturas dos Dentes/fisiopatologia , Suporte de Carga , Animais , Fenômenos Biomecânicos/métodos , Força Compressiva , Simulação por Computador , Dentina/diagnóstico por imagem , Dentina/patologia , Elasticidade , Elefantes , Dureza , Radiografia , Estresse Mecânico , Fraturas dos Dentes/diagnóstico por imagem , Fraturas dos Dentes/patologia
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