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1.
FASEB J ; 33(1): 88-100, 2019 01.
Artigo em Inglês | MEDLINE | ID: mdl-29957057

RESUMO

Zinc finger BED domain containing protein 6 ( Zbed6) has evolved from a domesticated DNA transposon and encodes a transcription factor unique to placental mammals. The aim of the present study was to investigate further the role of ZBED6 in insulin-producing cells, using mouse MIN6 cells, and to evaluate the effects of Zbed6 knockdown on basal ß-cell functions, such as morphology, transcriptional regulation, insulin content, and release. Zbed6-silenced cells and controls were characterized with a range of methods, including RNA sequencing, chromatin immunoprecipitation sequencing, insulin content and release, subplasma membrane Ca2+ measurements, cAMP determination, and morphologic studies. More than 700 genes showed differential expression in response to Zbed6 knockdown, which was paralleled by increased capacity to generate cAMP, as well as by augmented subplasmalemmal calcium concentration and insulin secretion in response to glucose stimulation. We identified >4000 putative ZBED6-binding sites in the MIN6 genome, with an enrichment of ZBED6 sites at upregulated genes, such as the ß-cell transcription factors v-maf musculoaponeurotic fibrosarcoma oncogene homolog A and Nk6 homeobox 1. We also observed altered morphology/growth patterns, as indicated by increased cell clustering, and in the appearance of axon-like Neurofilament, medium polypeptide and tubulin ß 3, class III-positive protrusions. We conclude that ZBED6 acts as a transcriptional regulator in MIN6 cells and that its activity suppresses insulin production, cell aggregation, and neuronal-like differentiation.-Wang, X., Jiang, L., Wallerman, O., Younis, S., Yu, Q., Klaesson, A., Tengholm, A., Welsh, N., Andersson, L. ZBED6 negatively regulates insulin production, neuronal differentiation, and cell aggregation in MIN6 cells.


Assuntos
Diferenciação Celular , Células Secretoras de Insulina/patologia , Insulina/metabolismo , Insulinoma/patologia , Neurônios/patologia , Neoplasias Pancreáticas/patologia , Proteínas Repressoras/metabolismo , Animais , Sítios de Ligação , Adesão Celular , Agregação Celular , Regulação da Expressão Gênica , Inativação Gênica , Glucose/administração & dosagem , Sequenciamento de Nucleotídeos em Larga Escala , Células Secretoras de Insulina/metabolismo , Insulinoma/metabolismo , Camundongos , Neurônios/metabolismo , Neoplasias Pancreáticas/metabolismo , Proteínas Repressoras/antagonistas & inibidores , Proteínas Repressoras/genética , Transcrição Gênica , Células Tumorais Cultivadas
2.
J Exp Biol ; 222(Pt 5)2019 03 08.
Artigo em Inglês | MEDLINE | ID: mdl-30718374

RESUMO

Functional validation of candidate genes involved in adaptation and speciation remains challenging. Here, we exemplify the utility of a method quantifying individual mRNA transcripts in revealing the molecular basis of divergence in feather pigment synthesis during early-stage speciation in crows. Using a padlock probe assay combined with rolling circle amplification, we quantified cell-type-specific gene expression in the histological context of growing feather follicles. Expression of Tyrosinase Related Protein 1 (TYRP1), Solute Carrier Family 45 member 2 (SLC45A2) and Hematopoietic Prostaglandin D Synthase (HPGDS) was melanocyte-limited and significantly reduced in follicles from hooded crow, explaining the substantially lower eumelanin content in grey versus black feathers. The central upstream Melanocyte Inducing Transcription Factor (MITF) only showed differential expression specific to melanocytes - a feature not captured by bulk RNA-seq. Overall, this study provides insight into the molecular basis of an evolutionary young transition in pigment synthesis, and demonstrates the power of histologically explicit, statistically substantiated single-cell gene expression quantification for functional genetic inference in natural populations.


Assuntos
Corvos/fisiologia , Plumas/fisiologia , Regulação da Expressão Gênica , Especiação Genética , Pigmentação/genética , Pigmentos Biológicos/genética , RNA Mensageiro/genética , Animais , Cor , Corvos/genética , Plumas/crescimento & desenvolvimento , Melanócitos/metabolismo , Pigmentos Biológicos/biossíntese
3.
Curr Top Microbiol Immunol ; 377: 111-26, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-23921974

RESUMO

The fate of the cell is governed by interactions among proteins, nucleic acids, and other biomolecules. It is vital to look at these interactions in a cellular environment if we want to increase our understanding of cellular processes. Herein we will describe how the in situ proximity ligation assay (in situ PLA) can be used to visualize protein interactions in fixed cells and tissues. In situ PLA is a novel technique that uses DNA, together with DNA modifying processes such as ligation, cleavage, and polymerization, as tools to create surrogate markers for protein interactions of interest. Different in situ PLA designs make it possible not only to detect protein-protein interactions but also post-translational modifications and interactions of proteins with nucleic acids. Flexibility in DNA probe design and the multitude of different DNA modifying enzymes provide the basis for modifications of the method to make it suitable to use in many applications. Furthermore, examples of how in situ PLA can be combined with other methods for a comprehensive view of the cellular activity status are discussed.


Assuntos
Mapeamento de Interação de Proteínas/métodos , Proteínas/metabolismo , Animais , DNA/genética , DNA/metabolismo , Humanos , Ligação Proteica , Processamento de Proteína Pós-Traducional , Proteínas/genética
4.
Nat Commun ; 13(1): 4755, 2022 08 13.
Artigo em Inglês | MEDLINE | ID: mdl-35963857

RESUMO

Determining the levels of protein-protein interactions is essential for the analysis of signaling within the cell, characterization of mutation effects, protein function and activation in health and disease, among others. Herein, we describe MolBoolean - a method to detect interactions between endogenous proteins in various subcellular compartments, utilizing antibody-DNA conjugates for identification and signal amplification. In contrast to proximity ligation assays, MolBoolean simultaneously indicates the relative abundances of protein A and B not interacting with each other, as well as the pool of A and B proteins that are proximal enough to be considered an AB complex. MolBoolean is applicable both in fixed cells and tissue sections. The specific and quantifiable data that the method generates provide opportunities for both diagnostic use and medical research.


Assuntos
Mapeamento de Interação de Proteínas , Proteínas , Mapeamento de Interação de Proteínas/métodos , Proteínas/metabolismo , Transdução de Sinais
5.
Front Cell Dev Biol ; 8: 579, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32733888

RESUMO

Many newly identified solute carriers (SLCs) and putative transporters have the possibility to be intricately involved in glucose metabolism. Here we show that many transporters of this type display a high degree of regulation at both mRNA and protein level following no or low glucose availability in mouse cortex cultures. We show that this is also the case in Drosophila melanogaster subjected to starvation or diets with different sugar content. Interestingly, re-introduction of glucose to media, or refeeding flies, normalized the gene expression of a number of the targets, indicating a fast and highly dynamic control. Our findings demonstrate high conservation of these transporters and how dependent both cell cultures and organisms are on gene and protein regulation during metabolic fluctuations. Several transporter genes were regulated simultaneously maybe to initiate alternative metabolic pathways as a response to low glucose levels, both in the cell cultures and in D. melanogaster. Our results display that newly identified SLCs of Major Facilitator Superfamily type, as well as the putative transporters included in our study, are regulated by glucose availability and could be involved in several cellular aspects dependent of glucose and/or its metabolites. Recently, a correlation between dysregulation of glucose in the central nervous system and numerous diseases such as obesity, type 2 diabetes mellitus as well as neurological disease such as Alzheimer's and Parkinson's diseases indicate a complex regulation and fine tuning of glucose levels in the brain. The fact that almost one third of transporters and transporter-related proteins remain orphans with unknown or contradictive substrate profile, location and function, pinpoint the need for further research about them to fully understand their mechanistic role and their impact on cellular metabolism.

6.
Sci Rep ; 8(1): 5400, 2018 03 29.
Artigo em Inglês | MEDLINE | ID: mdl-29599435

RESUMO

We have redesigned probes for in situ proximity ligation assay (PLA), resulting in more efficient localized detection of target proteins. In situ PLA depends on recognition of target proteins by pairs of antibody-oligonucleotide conjugates (PLA probes), which jointly give rise to DNA circles that template localized rolling circle amplification reactions. The requirement for dual recognition of the target proteins improves selectivity by ignoring any cross-reactivity not shared by the antibodies, and it allows detection of protein-protein interactions and post-translational modifications. We herein describe an improved design of the PLA probes -UnFold probes - where all elements required for formation of circular DNA strands are incorporated in the probes. Premature interactions between the UnFold probes are prevented by including an enzymatic "unfolding" step in the detection reactions. This allows DNA circles to form by pairs of reagents only after excess reagents have been removed. We demonstrate the performance of UnFold probes for detection of protein-protein interactions and post-translational modifications in fixed cells and tissues, revealing considerably more efficient signal generation. We also apply the UnFold probes to detect IL-6 in solution phase after capture on solid supports, demonstrating increased sensitivity over both normal sandwich enzyme-linked immunosorbent assays and conventional PLA assays.


Assuntos
Anticorpos Imobilizados/química , Oligonucleotídeos/química , Proteínas/análise , Anticorpos Imobilizados/imunologia , Sequência de Bases , Caderinas/química , Caderinas/metabolismo , Linhagem Celular , DNA Circular/química , DNA Circular/metabolismo , Corantes Fluorescentes/química , Humanos , Microscopia de Fluorescência , Conformação de Ácido Nucleico , Mapeamento de Interação de Proteínas/métodos , Processamento de Proteína Pós-Traducional , Proteínas/imunologia , Pele/metabolismo , Pele/patologia , beta Catenina/química , beta Catenina/metabolismo
8.
Open Biol ; 7(9)2017 09.
Artigo em Inglês | MEDLINE | ID: mdl-28878041

RESUMO

Solute carriers (SLCs) are vital as they are responsible for a major part of the molecular transport over lipid bilayers. At present, there are 430 identified SLCs, of which 28 are called atypical SLCs of major facilitator superfamily (MFS) type. These are MFSD1, 2A, 2B, 3, 4A, 4B, 5, 6, 6 L, 7, 8, 9, 10, 11, 12, 13A, 14A and 14B; SV2A, SV2B and SV2C; SVOP and SVOPL; SPNS1, SPNS2 and SPNS3; and UNC93A and UNC93B1. We studied their fundamental properties, and we also included CLN3, an atypical SLC not yet belonging to any protein family (Pfam) clan, because its involvement in the same neuronal degenerative disorders as MFSD8. With phylogenetic analyses and bioinformatic sequence comparisons, the proteins were divided into 15 families, denoted atypical MFS transporter families (AMTF1-15). Hidden Markov models were used to identify orthologues from human to Drosophila melanogaster and Caenorhabditis elegans Topology predictions revealed 12 transmembrane segments (for all except CLN3), corresponding to the common MFS structure. With single-cell RNA sequencing and in situ proximity ligation assay on brain cells, co-expressions of several atypical SLCs were identified. Finally, the transcription levels of all genes were analysed in the hypothalamic N25/2 cell line after complete amino acid starvation, showing altered expression levels for several atypical SLCs.


Assuntos
Evolução Molecular , Proteínas de Membrana Transportadoras/química , Proteínas de Membrana Transportadoras/classificação , Neurônios/metabolismo , Sequência de Aminoácidos , Animais , Transporte Biológico , Caenorhabditis elegans/genética , Caenorhabditis elegans/metabolismo , Galinhas/genética , Galinhas/metabolismo , Sequência Conservada , Drosophila melanogaster/genética , Drosophila melanogaster/metabolismo , Humanos , Hipotálamo/citologia , Hipotálamo/metabolismo , Cadeias de Markov , Proteínas de Membrana Transportadoras/genética , Proteínas de Membrana Transportadoras/metabolismo , Camundongos , Neurônios/citologia , Filogenia , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/metabolismo , Alinhamento de Sequência , Análise de Sequência de RNA , Homologia de Sequência de Aminoácidos , Análise de Célula Única , Transcrição Gênica , Peixe-Zebra/genética , Peixe-Zebra/metabolismo
9.
Sci Rep ; 5: 12317, 2015 Jul 23.
Artigo em Inglês | MEDLINE | ID: mdl-26202090

RESUMO

Rolling circle amplification (RCA) for generation of distinct fluorescent signals in situ relies upon the self-collapsing properties of single-stranded DNA in commonly used RCA-based methods. By introducing a cross-hybridizing DNA oligonucleotide during rolling circle amplification, we demonstrate that the fluorophore-labeled RCA products (RCPs) become smaller. The reduced size of RCPs increases the local concentration of fluorophores and as a result, the signal intensity increases together with the signal-to-noise ratio. Furthermore, we have found that RCPs sometimes tend to disintegrate and may be recorded as several RCPs, a trait that is prevented with our cross-hybridizing DNA oligonucleotide. These effects generated by compaction of RCPs improve accuracy of visual as well as automated in situ analysis for RCA based methods, such as proximity ligation assays (PLA) and padlock probes.


Assuntos
DNA Circular/química , DNA Circular/genética , DNA de Cadeia Simples/química , DNA de Cadeia Simples/genética , Técnicas de Amplificação de Ácido Nucleico/métodos , Análise de Sequência de DNA/métodos , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Razão Sinal-Ruído
10.
Nat Commun ; 6: 7294, 2015 Jun 12.
Artigo em Inglês | MEDLINE | ID: mdl-26065580

RESUMO

Sensitive detection of protein interactions and post-translational modifications of native proteins is a challenge for research and diagnostic purposes. A method for this, which could be used in point-of-care devices and high-throughput screening, should be reliable, cost effective and robust. To achieve this, here we design a method (proxHCR) that combines the need for proximal binding with hybridization chain reaction (HCR) for signal amplification. When two oligonucleotide hairpins conjugated to antibodies bind in close proximity, they can be activated to reveal an initiator sequence. This starts a chain reaction of hybridization events between a pair of fluorophore-labelled oligonucleotide hairpins, generating a fluorescent product. In conclusion, we show the applicability of the proxHCR method for the detection of protein interactions and posttranslational modifications in microscopy and flow cytometry. As no enzymes are needed, proxHCR may be an inexpensive and robust alternative to proximity ligation assays.


Assuntos
Hibridização de Ácido Nucleico , Oligonucleotídeos/química , Fator de Crescimento Epidérmico/química , Receptores ErbB/química , Citometria de Fluxo , Corantes Fluorescentes/química , Ligação Proteica
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