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1.
Science ; 269(5228): 1273-8, 1995 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-7544493

RESUMO

The adhesion domain of human CD2 bears a single N-linked carbohydrate. The solution structure of a fragment of CD2 containing the covalently bound high-mannose N-glycan [-(N-acetylglucosamine)2-(mannose)5-8] was solved by nuclear magnetic resonance. The stem and two of three branches of the carbohydrate structure are well defined and the mobility of proximal glycan residues is restricted. Mutagenesis of all residues in the vicinity of the glycan suggests that the glycan is not a component of the CD2-CD58 interface; rather, the carbohydrate stabilizes the protein fold by counterbalancing an unfavorable clustering of five positive charges centered about lysine-61 of CD2.


Assuntos
Antígenos CD2/química , Oligossacarídeos/química , Conformação Proteica , Acetilglucosamina/química , Sequência de Aminoácidos , Animais , Antígenos CD/metabolismo , Sítios de Ligação , Antígenos CD2/metabolismo , Antígenos CD58 , Células CHO , Configuração de Carboidratos , Sequência de Carboidratos , Adesão Celular , Cricetinae , Glicosilação , Humanos , Espectroscopia de Ressonância Magnética , Glicoproteínas de Membrana/metabolismo , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida
2.
J Neurosci ; 21(12): 4154-61, 2001 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-11404400

RESUMO

The Kv4 subfamily of voltage-gated potassium channels is responsible for the transient A-type potassium current that operates at subthreshold membrane potentials to control membrane excitability. Arachidonic acid was shown recently to modulate both the peak amplitude and kinetics of the hippocampal A-current. However, in Xenopus oocytes, arachidonic acid only inhibited the peak amplitude of Kv4 current without modifying its kinetics. These results suggest the existence of Kv4 auxiliary subunit(s) in native cells. We report here a K-channel interacting protein (KChIP)-dependent kinetic modulation of Kv4.2 current in Chinese hamster ovary cells and Kv4.2 and Kv4.3 currents in Xenopus oocytes by arachidonic acid at physiological concentrations. This concentration-dependent effect of arachidonic acid resembled that observed in cerebellar granule neurons and was fully reversible. Other fatty acids, including a nonhydrolyzable inhibitor of both lipooxygenase and cyclooxygenase, 5,8,11,14-eicosatetraynoic acid (ETYA), also mimicked arachidonic acid in modulating Kv4.3 and Kv4.3/KChIP1 currents. Compared with another transient potassium current formed by Kv1.1/Kvbeta1, Kv4.3/KChIP1 current was much more sensitive to arachidonic acid. Association between KChIP1 and Kv4.2 or Kv4.3 was not altered in the presence of 10 microm ETYA as measured by immunoprecipitation and association-dependent growth in yeast. Our data suggest that the KChIP proteins represent a molecular entity for the observed difference between arachidonic acid effects on A-current kinetics in heterologous cells and in native cells and are consistent with the notion that KChIP proteins modulate the subthreshold A-current in neurons.


Assuntos
Ácido Araquidônico/farmacologia , Proteínas de Ligação ao Cálcio/metabolismo , Canais de Potássio de Abertura Dependente da Tensão da Membrana , Canais de Potássio/metabolismo , Ácido 5,8,11,14-Eicosatetrainoico/farmacologia , Animais , Células CHO , Proteínas de Ligação ao Cálcio/genética , Células Cultivadas , Cricetinae , Relação Dose-Resposta a Droga , Ácidos Graxos/farmacologia , Humanos , Proteínas Interatuantes com Canais de Kv , Potenciais da Membrana/efeitos dos fármacos , Potenciais da Membrana/fisiologia , Neurônios/citologia , Neurônios/efeitos dos fármacos , Neurônios/metabolismo , Oócitos/metabolismo , Técnicas de Patch-Clamp , Potássio/metabolismo , Canais de Potássio/genética , Ligação Proteica/efeitos dos fármacos , Subunidades Proteicas , Ratos , Ratos Sprague-Dawley , Canais de Potássio Shal , Transfecção , Técnicas do Sistema de Duplo-Híbrido , Xenopus laevis
3.
Protein Sci ; 10(2): 226-35, 2001 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-11266609

RESUMO

Chorionic gonadotropin (hCG) is a heterodimeric placental glycoprotein hormone essential for human reproduction. Twenty hCG beta-subunit residues, termed the seatbelt, are wrapped around alpha-subunit loop 2 (alpha 2) and their positions "latched" by a disulfide formed by cysteines at the end of the seatbelt (Cys 110) and in the beta-subunit core (Cys 26). This unique arrangement explains the stability of the heterodimer but raises questions as to how the two subunits combine. The seatbelt is latched in the free beta-subunit. If the seatbelt remained latched during the process of subunit combination, formation of the heterodimer would require alpha 2 and its attached oligosaccharide to be threaded through a small beta-subunit hole. The subunits are known to combine during oxidizing conditions in vitro, and studies described here tested the idea that this requires transient disruption of the latch disulfide, possibly as a consequence of the thioredoxin activity reported in hCG. We observed that alkylating agents did not modify either cysteine in the latch disulfide (Cys 26 or Cys 110) during heterodimer formation in several oxidizing conditions and had minimal influence on these cysteines during combination in the presence of mild reductants (1--3 mM beta-mercaptoethanol). Reducing agents appeared to accelerate subunit combination by disrupting a disulfide (Cys 93--Cys 100) that forms a loop within the seatbelt, thereby increasing the size of the beta-subunit hole. We propose a mechanism for hCG assembly in vitro that depends on movements of alpha 2 and the seatbelt and suggest that the process of glycoprotein hormone subunit combination may be useful for studying the movements of loops during protein folding.


Assuntos
Gonadotropina Coriônica/química , Alquilantes/farmacologia , Western Blotting , Cisteína/química , Dimerização , Dissulfetos , Glicosilação , Humanos , Espectrometria de Massas , Peptídeos/química , Ligação Proteica , Conformação Proteica , Estrutura Secundária de Proteína , Tiorredoxinas/metabolismo , Fatores de Tempo
5.
Clin Immunol Immunopathol ; 31(2): 212-21, 1984 May.
Artigo em Inglês | MEDLINE | ID: mdl-6713741

RESUMO

The cerebrospinal fluid (CSF) and serum from 64 patients with multiple sclerosis (MS) and 47 patients with monosymptomatic optic neuritis (ON) were analyzed for the distribution of allotypic determinants on IgG and compared to similar samples from 51 patients with other neurological diseases (OND) as well as to serum samples from 97 healthy controls. The results indicate a significantly increased frequency of the haplotypes Gm a;g and Gm a,x;g among MS patients (P = 0.024) with an associated increase in relative risk for MS among individuals with the Gm a,(x);g haplotypes compared to those individuals without them (P = 0.014). Among MS patients, those with the Gm a,(x);g haplotypes had significantly higher CSF levels of IgG than those without (P = 0.016); levels of serum IgG did not covary with Gm haplotype. Two-way analysis of variance indicates that familial cases have significantly higher levels of CSF IgG than nonfamilial cases (P less than 0.001) and that familial cases with the Gm a,(x);g haplotypes have the highest CSF IgG levels (P less than 0.005). There was no correlation between Gm haplotype and CSF or serum IgG levels in patients with ON or OND. The allotype effects were independent of age at onset and duration of disease. In all patients, regardless of disease classification, the phenotypes found in serum samples were identical to those found in CSF samples. The data presented support the hypothesis that the etiology of MS has as one of its parameters an immunoregulatory/immunogenetic factor. The successful analysis of these various parameters will provide useful information not only about MS but also about general principles of human immune responsiveness.


Assuntos
Alótipos de Imunoglobulina/genética , Imunoglobulina G/líquido cefalorraquidiano , Esclerose Múltipla/imunologia , Neurite Óptica/imunologia , Adolescente , Adulto , Idoso , Suscetibilidade a Doenças , Feminino , Genes Reguladores , Antígenos HLA/genética , Haploidia , Humanos , Imunoglobulina G/genética , Masculino , Pessoa de Meia-Idade , Esclerose Múltipla/líquido cefalorraquidiano , Esclerose Múltipla/genética , Neurite Óptica/líquido cefalorraquidiano , Neurite Óptica/genética , Fenótipo
6.
J Immunol ; 150(11): 4833-43, 1993 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-7684413

RESUMO

The CD2 molecule plays an important role in T cell adhesion by interacting with the ligands CD58 (LFA-3) and CD59. In order to detect additional ligands for CD2, potentially of low binding affinity, we have prepared a highly fluorescent, multimeric form of rCD2 whose binding to cells can be quantified by flow cytometry. Initial studies demonstrated that binding of multimeric rCD2 to cells was CD2-specific, concentration and time dependent, and saturable. The negative charge on cells was also found to play a critical role in the efficiency of multimeric rCD2 binding. Analysis of binding of multimeric rCD2 to 17 CD58+ cell types revealed that only 8 of the cells exhibited binding. Failure of multimeric rCD2 to interact with the other cells could not be explained by differences in CD58 expression, suggesting that, in terms of CD2 binding, there are qualitative differences in CD58 on different cell types. Binding of multimeric rCD2 to six of the seven reactive cells was virtually totally inhibited by CD58 mAb pretreatment, whereas binding to the erythroleukemic line K562 was only partially blocked, suggesting the existence of another CD2 ligand. Subsequent studies demonstrated that the putative new ligand is not CD59, and that it interacts with a different region of the CD2 molecule than CD58, probably a site located between the T11(1) and T11(2) epitopes. The binding affinity of CD2 for the new ligand is 10-fold lower than for CD58 and, based on studies with truncated rCD2, the binding site for the new ligand is located within the amino-terminal 105 amino acids of the CD2 polypeptide. Unlike CD58, the new ligand is tunicamycin sensitive suggesting that it contains a N-linked carbohydrate structure that is essential for functional activity.


Assuntos
Antígenos de Diferenciação de Linfócitos T/análise , Moléculas de Adesão Celular/análise , Receptores Imunológicos/análise , Anticorpos Monoclonais/imunologia , Antígenos CD/análise , Antígenos CD/fisiologia , Antígenos CD2 , Antígenos CD58 , Linhagem Celular , Glicosilação , Humanos , Substâncias Macromoleculares , Glicoproteínas de Membrana/análise , Glicoproteínas de Membrana/fisiologia , Polieletrólitos , Polímeros/farmacologia , Ensaio Radioligante , Proteínas Recombinantes/metabolismo
7.
Biochemistry ; 32(41): 10995-1006, 1993 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-8105887

RESUMO

Human CD2, a glycosylated transmembrane receptor found on all T-lymphocytes, plays a key role in facilitating cellular adhesion between T-cells and target cells or antigen-presenting cells by binding to its counter receptor CD58 (LFA-3) present on the surface of those cells. All CD2 adhesion functions are localized within the amino-terminal 105-residue domain, which contains a single high mannose N-glycan required for maintaining both the conformational stability and CD58 binding properties of the glycoprotein. In order to better understand the structural basis for CD2-CD58-mediated adhesion and the critical role of the carbohydrate moiety in maintaining the functional stability of the molecule, we have determined the secondary structure of the N-glycosylated adhesion domain of human CD2 (hu-sCD2(105)) using NMR spectroscopy. Most of the 1H resonance assignments have been obtained from 1H-1H homonuclear 2D NMR spectra, which were further extended by applying 1H-15N heteronuclear 2D experiments on a hu-sCD2(105) sample selectively labeled with [15N]lysine. Thus, 98% of all backbone 1H resonances and over 80% of all side chain 1H resonances have been assigned. An overall topology characteristic of an immunoglobulin variable domain is observed, which consists of two beta-sheets comprised of three (residues 16-20, 67-71, and 60-63) and five (residues 94-103, 80-86, 32-37, 45-47, and 53-55) antiparallel beta-strands, respectively, with a hydrophobic core sandwiched between them. A ninth beta-strand (residues 7-12) makes parallel contacts to the carboxy-terminal beta-strand. NOEs between the N-linked glycan and the protein have tentatively been identified.


Assuntos
Antígenos de Diferenciação de Linfócitos T/química , Adesão Celular , Espectroscopia de Ressonância Magnética , Estrutura Secundária de Proteína , Receptores Imunológicos/química , Sequência de Aminoácidos , Sítios de Ligação , Antígenos CD2 , Glicosilação , Humanos , Dados de Sequência Molecular , Dobramento de Proteína
8.
J Biol Chem ; 267(31): 22428-34, 1992 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-1385399

RESUMO

The T-lymphocyte glycoprotein receptor, CD2, mediates cell-cell adhesion by binding to the surface molecule CD58 (LFA-3) on many cell types including antigen presenting cells. Two domains comprise the CD2 extracellular segment, with all adhesion functions localized to the amino-terminal domain that contains a single N-glycosylation site at Asn65. We have defined an important role for the N-linked glycans attached to Asn65 of this domain in mediating CD2-CD58 interactions and also characterize its N-glycotype structure. Analysis of deglycosylated soluble recombinant CD2 as well as a mutant transmembrane CD2 molecule containing a single Asn65-Gln65 substitution demonstrates that neither deglycosylated CD2 nor the mutant CD2 transmembrane receptor binds CD58 or monoclonal antibodies directed at native CD2 adhesion domain epitopes. Electrospray ionization-mass spectrometry demonstrates that high mannose oligosaccharides ((Man)nGlcNAc2, n = 5-9) are the only N-glycotypes occupying Asn65 when soluble CD2 is expressed in Chinese hamster ovary cells. Based on a model of human CD2 secondary structure, we propose that N-glycosylation is required for stabilizing domain 1 in the human receptor. Thus, N-glycosylation is essential for human CD2 adhesion functions.


Assuntos
Antígenos de Diferenciação de Linfócitos T/química , Moléculas de Adesão Celular/química , Receptores Imunológicos/química , Sequência de Aminoácidos , Antígenos CD/metabolismo , Antígenos de Diferenciação de Linfócitos T/metabolismo , Sequência de Bases , Antígenos CD2 , Antígenos CD58 , Glicosilação , Humanos , Espectrometria de Massas , Glicoproteínas de Membrana/química , Glicoproteínas de Membrana/metabolismo , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Oligodesoxirribonucleotídeos/química , Receptores Imunológicos/metabolismo , Formação de Roseta , Relação Estrutura-Atividade
9.
Cell Immunol ; 149(1): 24-38, 1993 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-7685660

RESUMO

The CD2 (T11) molecule belongs to a family of cell-surface glycoproteins that function as adhesion molecules in the immune system. Human CD2 is found exclusively on cells of the T lineage: peripheral T lymphocytes, NK cells, and thymocytes. CD2 binds specifically to the surface glycoprotein LFA-3. CD2/LFA-3 adhesion is the basis for the formation of rosettes between T cells and sheep erythrocytes (SRBC) which bear the sheep homologue of LFA-3. More importantly, CD2/LFA-3 adhesion functions in the immune system to augment T cell activation; it initiates conjugate formation between participating T cells and antigen-presenting cells (APC). We investigated the effects of soluble forms of CD2 (sCD2), produced in either baculovirus or CHO expression systems, on the rosetting of T cells with SRBC and on the activation of T cells by antigen plus major histocompatibility complex (MHC) molecules. Rosette formation between T cells and SRBC was completely inhibited by as little as 1 microM sCD2. Furthermore, sCD2 effectively inhibited (at micromolar concentrations) the T cell proliferative response to recall antigens including rubella, tetanus toxoid, and herpes simplex virus (HSV-1), as well as alloantigens in a mixed lymphocyte culture. These findings are consistent with the notion that the CD2/LFA-3 interaction augments antigen-specific T cell functions. The use of a CD2 "decoy" molecule rather than anti-CD2 or anti-LFA-3 antibodies to block the CD2/LFA-3 interaction rules out secondary antibody effects, via the Fc portion, as the basis for inhibition of T cell activation and directly stresses the importance of this adhesion interaction in T cell responses.


Assuntos
Antígenos de Diferenciação de Linfócitos T/fisiologia , Adesão Celular/efeitos dos fármacos , Ativação Linfocitária , Receptores Imunológicos/fisiologia , Linfócitos T/imunologia , Antígenos CD/fisiologia , Antígenos de Diferenciação de Linfócitos T/química , Antígenos Virais/imunologia , Antígenos CD2 , Antígenos CD58 , Humanos , Memória Imunológica , Técnicas In Vitro , Teste de Cultura Mista de Linfócitos , Glicoproteínas de Membrana/fisiologia , Receptores Imunológicos/química , Proteínas Recombinantes , Formação de Roseta , Solubilidade , Toxoide Tetânico/imunologia
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