RESUMO
The qualitative composition and zeta potential of magnetite nanoparticles (size 4.2±1.2 nm) obtained by co-precipitation method were determined by X-ray and diffraction dynamic light scattering. The zeta potential of Fe3O4 particles was -15.1±4.5 mV. The possibility of interaction of magnetite nanoparticles with human blood plasma proteins and hemoglobin as well as with erythrocyte membranes was demonstrated by spectrophotometry, electrophoresis, and fluorescence methods. No changes in the sizes of hemoglobin molecules and plasma proteins after their modification by Fe3O4 particles were detected. The possibility of modifying the structural state of erythrocyte membranes in the presence of magnetite nanoparticles was demonstrated by means of fluorescent probe 1-anilinonaphthalene-8-sulfonate.
Assuntos
Hemoglobinas , Nanopartículas de Magnetita , Humanos , Nanopartículas de Magnetita/química , Hemoglobinas/química , Membrana Eritrocítica/efeitos dos fármacos , Membrana Eritrocítica/química , Membrana Eritrocítica/metabolismo , Tamanho da Partícula , Proteínas Sanguíneas/química , Naftalenossulfonato de Anilina/química , Difração de Raios X , Óxido Ferroso-Férrico/química , Corantes Fluorescentes/químicaRESUMO
Exposure of human lymphocytes to silver nanoparticles (2-12 nm) reduced viability of cells and RNA, reduced activities of lactate dehydrogenase, glutathione reductase, and cytosolic calcium, increased ROS content in cells, affected surface architectonics of cells and changed hydrophobicity and charge of their plasma membranes. Silver nanoparticles triggered the process of cellular death by the mechanism of ETosis that is accompanied by chromatin release into the extracellular environment and formation of extracellular traps.
Assuntos
Linfócitos/metabolismo , Nanopartículas Metálicas/química , Prata/química , Adolescente , Adulto , Cálcio/metabolismo , Armadilhas Extracelulares/metabolismo , Glutationa Redutase/metabolismo , Humanos , L-Lactato Desidrogenase/metabolismo , Masculino , Microscopia de Fluorescência , Espécies Reativas de Oxigênio/metabolismo , Adulto JovemRESUMO
The influence of a wide range of doses of UV-light (240-390 nm) on the expression level of receptor complex molecules (CD3, CD4, CD8 markers) on the membrane surface of human blood T-lymphocytes has been studied using flow cytometry and enzyme linked immunosorbent assay. UV-light at small and medium doses (15 1, 453 and 906 J/m2) has been established to have a unidirectional (activating) effect on the expression level of receptor complex molecules, and at a high dose of 1359 J/m2 it can either increase (CD4 and CD8 markers) or reduce (CD3 complexes) the quantity of the analyzed molecules on the membrane surface of T-lymphocytes.
Assuntos
Complexo CD3/biossíntese , Antígenos CD4/biossíntese , Antígenos CD8/biossíntese , Receptores de Antígenos de Linfócitos T/biossíntese , Linfócitos T/efeitos da radiação , Raios Ultravioleta , Membrana Celular/imunologia , Membrana Celular/metabolismo , Membrana Celular/efeitos da radiação , Células Cultivadas , Relação Dose-Resposta à Radiação , Citometria de Fluxo , Humanos , Técnicas Imunoenzimáticas , Linfócitos T/imunologia , Linfócitos T/metabolismoRESUMO
The influence of leucocytic alpha-interferon in concentrations of 0.01, 0.1, 1, 10, and 100 IU/ml on the expression of CD3 complexes by native and photomodified T lymphocytes of human blood has been studied. It was found that the irradiation of suspensions of T cells by UV-light in doses of 151-906 J/m2 increases, and in a dose of 1359 J/m2, reduces the quantity of CD3 complexes on the surface of their membranes. It was shown that the injection of cytokine in concentrations of 0.01-100 IU/ml into a suspension of irradiated T lymphocytes levels off the immunosuppressive action of maximal doses of UV-radiation (1359 J/m2), inducing the recovery and increase in their antigen-recognizing capacity. The data on the antigen-recognizing capacity of UV-irradiated T lymphocytes of human blood, modified by alpha-interferon, enable one to predict the results of seances of autotransfusion of UV-irradiated blood in the case of combined use of UV-irradiation of blood and cytokinotherapy.