TYPE 2 DIABETES MELLITUS PREVALENCE IN PATIENTS WITH CARDIOVASCULAR DIEASES.

OBJECTIVE: The aim: To estimate type 2 diabetes mellitus (T2DM) prevalence in patients who came for a consultation to the private practice cardiologist. PATIENTS AND METHODS: Materials and methods: 97 patients with cardiovascular diseases (СVD), visited a cardiologist in private medical center, were included (mean age 49,5 (42,8; 58,3) years, 43 (44.3 %) males) and T2DM prevalence was estimated. RESULTS: Results: 84 (86.6 %) patients had arterial hypertension, 19 patients (19.6 %) had coronary artery disease. Mean body mass index was 29,4 (25,2; 33,4) kg/m2. Waist circumference above recommended was observed in 78.4 %. 30 рatients (30.9 %) were overweight, 44.3 % had abdominal obesity. Almost all patients had atherogenic dyslipidemia. Total T2DM prevalence was 19.6 %, including cases of first-diagnosed diabetes (17.5 %). CONCLUSION: Conclusions: T2DM and prediabetes prevalence in our sample of patients with CVD was higer than described for the general population. Рatients with cardiovascular diseases require careful assessment of diabetes risk factors for its timely detection and for possiblу improving the outcomes.

Eradication of avian leukosis virus subgroups J and K in broiler cross chickens by selection against infected birds using multilocus PCR.

The avian leukosis virus (ALV) is a serious threat to sustainable and economically viable commercial poultry management world-wide. Active infections can result in more than 20% flock loss, resulting in significant economic damage. ALV detection and elimination from flocks and breeding programs is complicated by high sequence variability and the presence of endogenous virus copies which show up as false positives in assays. Previously-developed approaches to virus detection are either too labor-intensive to implement on an industrial scale or suffer from high false negative or positive rates. We developed a novel multi-locus multiplex quantitative real-time PCR system to detect viruses belonging to the J and K genetic subgroups that are particularly prevalent in our region. We used this system to eradicate ALV from our broiler breeding program comprising thousands of individuals. Our approach can be generalized to other ALV subgroups and other highly genetically diverse pathogens.