Determination of membrane capacitance and cytoplasm conductivity by simultaneous electrorotation.

Membrane capacitances and cytoplasm conductivities of hematopoietic cells were investigated by simultaneous electrorotation (ROT) systems of multiple cells. Simultaneous ROT was achieved by the rotation of electric fields in grid arrays formed with three-dimensional interdigitated array (3D-IDA) electrodes that can be easily fabricated using two substrates with IDA electrodes. When AC signals were applied to four microband electrodes with a 90° phase difference to each electrode, cells dispersed randomly in the 3D-IDA device started to rotate and moved to the center of each grid. Multiple cells were simultaneously rotated at the center of grids without friction from contact with other cells and substrates. The averages and variance of ROT rates of cells at each frequency can be measured during a single operation of the device within 5 min, resulting in the acquisition of ROT spectra. Membrane capacitances and cytoplasm conductivities of hematopoietic cells (K562 cells, Jurkat cells, and THP-1 cells) were determined by fitting ROT spectra obtained experimentally to the curves calculated theoretically. The values determined by using the simultaneous ROT systems well coincided with the values reported previously. The membrane capacitances and cytoplasm conductivities of WEHI-231 cells were firstly determined to be 8.89 ± 0.25 mF m-2 and 0.28 ± 0.03 S m-1, respectively. Furthermore, the difference of the ROT rates based on the difference of the electric properties of cells was applied to discriminate the types of cells. The acquisition of rotation rates of multiple cells within a single operation makes the statistical analysis extremely profitable for determining the electrical properties of cells.

Diffraction light analysis method for a diffraction grating imaging lens.

We have developed a new method to analyze the amount and distribution of diffraction light for a diffraction grating lens. We have found that diffraction light includes each-order diffraction light and striped diffraction light. In this paper, we describe characteristics of striped diffraction light and suggest a way to analyze diffraction light. Our analysis method, which considers the structure of diffraction grating steps, can simulate the aberrations of an optical system, each-order diffraction light, and striped diffraction light simultaneously with high accuracy. A comparison between the simulation and experimental results is presented, and we also show how our analysis method can be used to optimize a diffraction grating lens with low flare light.

Phase function design of a diffraction grating lens for an optical imaging system from a Fraunhofer diffraction perspective.

The potential exists to apply diffraction gratings to optical imaging systems to improve camera resolution and shorten optical length. However, we have noted the generation of striped flare lights, which differ from unnecessary-order diffraction lights, under intense lighting. We have elucidated the generation principle of these new striped lights and have discovered that they are caused by narrow diffraction grating rings. In this paper, using an analysis based on Fraunhofer diffraction, we suggest a way of minimizing them by designing an appropriate phase function structure, and test the efficacy of this design using our own manufactured prototype.

Interference phase-contrast imaging technology without beam separation.

Interferometers are widely used in science and industry to measure small displacements, changes in refractive index, and surface irregularities. In all interferometers, including phase-contrast microscopes and DICs (differential interference contrast microscopes), light from a single source is split into two beams that travel along different optical paths. They are then recombined to produce interference. The fundamental operation of beam separation makes device configuration more complex and adds to the bulk of the equipment. In this study we propose a new method of observing phase-contrast images without beam separation by using self-interference inside a grating coupler structure disposed on the observation plane. We experimentally demonstrate that the self-interference principle can generate phase-contrast images using a simple configuration. From measurements using a multilevel phase plate, we confirm its phase-contrast depth resolution to approach one- tenth of a wavelength.

Non-contact acquisition of brain function using a time-extracted compact camera.

A revolution in functional brain imaging techniques is in progress in the field of neurosciences. Optical imaging techniques, such as high-density diffuse optical tomography (HD-DOT), in which source-detector pairs of probes are placed on subjects' heads, provide better portability than conventional functional magnetic resonance imaging (fMRI) equipment. However, these techniques remain costly and can only acquire images at up to a few measurements per square centimetre, even when multiple detector probes are employed. In this study, we demonstrate functional brain imaging using a compact and affordable setup that employs nanosecond-order pulsed ordinary laser diodes and a time-extracted image sensor with superimposition capture of scattered components. Our technique can simply and easily attain a high density of measurement points without requiring probes to be attached, and can directly capture two-dimensional functional brain images. We have demonstrated brain activity imaging using a phantom that mimics the optical properties of an adult human head, and with a human subject, have measured cognitive brain activation while the subject is solving simple arithmetical tasks.