Intracranial fetus-in-fetu with numerous fully developed organs.

Fetus in fetu (FIF) is an extremely rare anomaly featuring a monozygotic, diamniotic, parasitic twin, enclosed within its host twin. It is characterized by the presence of vertebrae and well-developed organs in a fetiform mass. Only 18 cases of intracranial FIF have been published. Of them, only five cases were prenatally detected. This study prenatally demonstrated triplet FIFs at 31 weeks within amniotic-like sac in the fetal skull, consisting of multiple well-defined organs. The FIF attached to the host twin via body stalk containing a single main feeding artery and vein, representing umbilical vessels. Surgical removal was performed at the age of two months. Pathological examination showed the triplet FIF, consisting of numerous well-developed organs (musculocutaneous-skeletal, nervous, respiratory, gastrointestinal systems etc.), with soft tissue/skin coverings, but no vertebral body was seen. Molecular genetic analysis revealed identical genetic mapping among the three FIFs and the host. This case provides strong evidence against Willis's hypothesis but supports Spencer's theory of abnormal twinning.

Accuracy of transcutaneous bilirubin measurement in term newborns.

OBJECTIVE: 1) To evaluate the accuracy of transcutaneous bilirubin (TcB) compared with serum bilirubin (TsB) in full-term infants, 2) To compare the accuracy of TcB reading from two, three, and four measurements and, 3) To compare the accuracy of TcB measured at the forehead and sternum. MATERIAL AND METHOD: Full-term infants who were diagnosed neonatal jaundice and underwent blood tests for TsB were eligible for enrollment. Infants who were born at gestational age less than 37 weeks, unstable, previously received phototherapy, or exchange transfusion were excluded. TcB was measured at forehead and sternum two, three, and four times at each site. TcB measurements were done within 30 minutes before or after blood sampling for TsB. RESULTS: The authors obtained 294 paired TcB-TsB from 257 full-term infants. TsB ranged from 1.60 to 21.18 mg/dL (mean 11.03, SD 2.73). The correlation coefficients between TcB at forehead and TsB were significant for two, three, and four measurements (r = 0.812, 0.800, and 0.800 respectively). TcB measured at sternum also had significant correlation with TsB (r = 0.829, 0.844, and 0.823 for two, three, and four measurements). TcB tended to underestimate TsB. Measurements at the sternum seemed to have better correlation with TsB than at the forehead. CONCLUSION: TcB has good correlation coefficient to TsB. Two measurements have enough accuracy to estimate TsB level and measurement at the sternum gives better correlation with TsB than at forehead.

Accuracy of various recent recommendations to estimate the optimal depth of orotracheal tube in Thai neonates.

BACKGROUND: Malposition of an endotracheal tube (ETT) may lead to many serious consequences. Recently, various methods have been proposed to estimate the proper position of orotracheal intubation (Pro-depth) for neonates. OBJECTIVES: To determine and compare the accuracy of various methods for estimating the Pro-depth and to define the most accurate method for the Asian population. METHOD: This cross-sectional study was conducted at the Chiang Mai University Hospital, Thailand in Asian neonates who required orotracheal intubation and their ETT's position were confirmed with anteroposterior chest radiographs. The estimated depths of orotracheal tubes (Est-depth) were calculated by using 4 methods: the rule of 7-8-9, NTL + 1 cm, corrected gestational age (GA)-based table, and body weight (BW)-based table. We defined the Pro-depth as the depth which provided the ETT's tip placed at mid trachea. The Pearson's correlation coefficient, the Bland-Altmann plot and mean differences with standard deviation (SD) between paired Est-depth by each method and Pro-depth were determined. New formulae were generated to estimate the Pro-depth based on a linear regression equation. The accuracy of each method to predict the optimal depth of orotracheal intubation (Opt-depth) was calculated. RESULTS: Fifty-eight Asian neonates were enrolled of which, 82.8% were Thai. The mean ± SD of GA and BW were 33 ± 5 weeks and 1562 ± 842 g, respectively. NTL + 1 cm and the Pro-depth showed the strongest correlation (r = 0.90, p-value < .01) and agreement with mean difference ± SD of 0.28 ± 0.53 cm. The accuracies of the rule of 7-8-9, NTL + 1cm, corrected GA-based and BW-based table to predict the Opt-depth were 56.9%, 63.8%, 62.1%, and 60.3%, respectively. We created two simple formulae for our population to increase the accuracy of NTL parameter: NTL + 1 cm for measured NTL ≤ 6.5 cm and NTL + 0.5 cm for measured NTL > 6.5 cm. This new method provided more accuracy (72.4%) with mean difference ± SD of -0.03 ± 0.53 cm. CONCLUSION: NTL was the most suitable parameter for estimating the Pro-depth. Our new modified NTL method should be used for Thai neonates with high accuracy and non-significantly underestimated trend. However, promptly clinical assessment and final confirmation by a chest radiography should be done in all intubated patients.

Reticulocyte hemoglobin concentration for screening iron deficiency in very low birth weight preterm neonates.

BACKGROUND: Preterm and low birth weight infants are at risk of iron deficiency. Reticulocyte hemoglobin concentration may be useful as a screening test to diagnose iron deficiency in preterm neonates. OBJECTIVE: To evaluate the accuracy and establish the reticulocyte hemoglobin concentration cutoff value for iron deficiency diagnosis in very low birth weight preterm neonates. METHOD: This study was conducted between May 2018 and March 2019 at Chiang Mai University Hospital. Preterm infants born at gestational age ≤34 weeks and birth weight ≤1500 g were enrolled. Blood samplings were obtained within the first 48 h of life. Iron deficiency was defined by using two or more of these following parameters: mean corpuscular volume <100 fL, transferrin saturation <16% and serum ferritin <30 µg/L. Neonatal anemia was defined as hemoglobin <15 g/dL. The optimum reticulocyte hemoglobin concentration cutoff values were performed by using predictive values and receiving operation characteristic analysis. RESULT: Fifty-seven preterm neonates were enrolled. Nine (15.7%) and three (5.3%) neonates had iron deficiency and iron deficiency anemia, respectively. The reticulocyte hemoglobin concentration cutoff value of <29 pg showed the optimum accuracy to diagnose iron deficiency in very low birth weight preterm neonates with sensitivity, specificity, positive and negative predictive values of 89%, 79%, 42% and 97%, respectively. CONCLUSION: Reticulocyte hemoglobin concentration can be used as a screening parameter to diagnose iron deficiency for VLBW preterm neonates. The optimum cutoff value which provided the acceptable accuracy was <29 pg.

Short-term outcome of perinatal hypoxic-ischaemic encephalopathy at Chiang Mai University Hospital, Thailand: a 15-year retrospective study.

BACKGROUND: The outcome of perinatal hypoxic-ischaemic encephalopathy (HIE) in middle-to-low-income countries varies between regions. OBJECTIVES: To determine the mortality and morbidity, and factors influencing the deaths of infants with perinatal HIE. METHODS: A retrospective study was conducted at Chiang Mai University Hospital, Thailand. Perinatal HIE infants of >35 weeks gestation, birthweight ≥2000 g and admitted during 2005-2019 were reviewed. Baseline Characteristics, clinical course and outcome at discharge were compared between the period before and after initiation of therapeutic hypothermia (TH). Risk of death in HIE infants who underwent TH was identified. RESULTS: A total of 162 HIE infants were included. Compared to the period before TH initiation, the mortality rate was significantly decreased in the TH period. (27% vs. 12.8%, p=0.04) Among 100 HIE infants who underwent TH, the mortality rates was 14%(14/100), of whom 2.5% (2/76) and 50% (12/24) were in the moderate and severe HIE groups. Apgar score at 5 mins ≤1, severe HIE, seizures, hypoglycaemia, organ involvement ≥ five sites, ammonia ≥100 umol/L, lactate ≥14 mmol/L, and requirement for two or more inotropic drugs were risks of death. Multivariate analysis demonstrated that severe HIE (aOR 732.8, 95% CI 4.7-114643, p=0.01) and a need for two or more inotropic drugs (aOR 45.7, 95% CI 1.5-1040, p=0.029) were significant factors for mortality. CONCLUSION: In the period of TH, perinatal HIE infants had decreased mortality. Severe HIE and a need for two or more inotropic drugs were associated with death in the infant with HIE who underwent TH.Abbreviations: AED: anti-epileptic drug; BW, birthweight; CI: confidence interval; CMU: Chiang Mai University; EEG: electro-encephalogram; GA: gestational age; HIE: hypoxic-ischaemic encephalopathy; IQR: interquartile range; NICU: neonatal intensive care unit; SD: standard deviation; TH: therapeutic hypothermia.

Accuracy of the Bilicare™ transcutaneous bilirubinometer as the predischarge screening tool for significant hyperbilirubinemia in healthy term and late preterm neonates.

Background: The Bilicare™ is a new device that measures transcutaneous bilirubin (TcB) level at the ear pinna. There are only few studies which have evaluated its accuracy in clinical practice.Objective: This study aims to determine the accuracy of Bilicare™ as a predischarge screening tool in late preterm and term neonates and to define the optimal cutoff point for determining the need to measure total serum bilirubin (TSB).Methods: The 35 weeks' gestation or more and healthy neonates who underwent predischarge TSB measurement were enrolled. Bilicare™ TcB was measured within 30 minutes of blood sampling. Paired TcB and TSB data were analyzed.Results: We collected 214 paired samples. Mean age (SD) at TcB measurement was 57.17 (7.47) hours. Mean TSB (SD) was 9.79 (2.83) mg/dL. TcB showed a significant correlation with TSB (r = 0.84, r2 = 0.7). The mean difference (SD) between TcB and TSB was 0.7 (0.21) mg/dL (95%CI 0.49-0.91). TcB tended to overestimate TSB level at the TSB values of <12 mg/dL but underestimate at the higher TSB level. The accuracy of using TcB values to detect neonates who required phototherapy was 92.5%. The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) were 78.3, 94.2, 62.1, and 97.3%, respectively. If TcB +3 mg/dL was applied as a cutoff point, the sensitivity, specificity, PPV, and NPV were 100, 53.9, 20.7, and 100%, respectively.Conclusions: Bilicare™ TcB and TSB measurements were well correlated. The TcB level +3 mg/dL could detect all neonates who had significant hyperbilirubinemia requiring phototherapy during their birth hospitalization.