RESUMO
High pressure reverse phase liquid chromatography has been employed to rapidly separate saturated and unsaturated fatty acids as the corresponding p-bromophenacyl esters. Through the use of a highly efficient C18 reverse phase column packing, it has also been possible to distinguish among geometrical and positional isomers of the unsaturated acids. The use of ultroviolet-sensitive esters has permitted the detection of low (nanogram range) concentrations of fatty acids. The time required for analysis has been further reduced by employing a novel and rapid method for the preparation of the esters.
Assuntos
Ácidos Graxos/isolamento & purificação , Cromatografia Líquida de Alta Pressão/métodos , Ésteres , Ácidos Graxos Insaturados/isolamento & purificação , Indicadores e ReagentesRESUMO
A rapid, mild, and convenient transesterification of triglycerides was obtained using 0.2 N-methanolic (m-trifluoro-methylphenyl) trimethylammonium hydroxide. The method yielded results comparable to those obtained using methanolic sodium methoxide.