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1.
Environ Microbiol ; 21(7): 2290-2306, 2019 07.
Artigo em Inglês | MEDLINE | ID: mdl-30927377

RESUMO

Antarctic subglacial environments host microbial ecosystems and are proving to be geochemically and biologically diverse. The Taylor Glacier, Antarctica, periodically expels iron-rich brine through a conduit sourced from a deep subglacial aquifer, creating a dramatic red surface feature known as Blood Falls. We used Illumina MiSeq sequencing to describe the core microbiome of this subglacial brine and identified previously undetected but abundant groups including the candidate bacterial phylum Atribacteria and archaeal phylum Pacearchaeota. Our work represents the first microbial characterization of samples collected from within a glacier using a melt probe, and the only Antarctic subglacial aquatic environment that, to date, has been sampled twice. A comparative analysis showed the brine community to be stable at the operational taxonomic unit level of 99% identity over a decade. Higher resolution sequencing enabled deconvolution of the microbiome of subglacial brine from mixtures of materials collected at the glacier surface. Diversity patterns between this brine and samples from the surrounding landscape provide insight into the hydrological connectivity of subglacial fluids to the surface polar desert environment. Understanding subice brines collected on the surfaces of thick ice covers has implications for analyses of expelled materials that may be sampled on icy extraterrestrial worlds.


Assuntos
Archaea/classificação , Bactérias/classificação , Camada de Gelo/microbiologia , Regiões Antárticas , Archaea/genética , Archaea/isolamento & purificação , Bactérias/genética , Bactérias/isolamento & purificação , Biodiversidade , Ecossistema , Sequenciamento de Nucleotídeos em Larga Escala , Camada de Gelo/química , Microbiota , RNA Ribossômico 16S/genética , Sais/análise
2.
Astrobiology ; 23(11): 1135-1152, 2023 11.
Artigo em Inglês | MEDLINE | ID: mdl-36576448

RESUMO

Ice-covered ocean worlds, such as the Jovian moon Europa, are some of the prime targets for planetary exploration due to their high astrobiological potential. While upcoming space exploration missions, such as the Europa Clipper and JUICE missions, will give us further insight into the local cryoenvironment, any conclusive life detection investigation requires the capability to penetrate and transit the icy shell and access the subglacial ocean directly. Developing robust, autonomous cryorobotic technology for such a mission constitutes an extremely demanding multistakeholder challenge and requires a concentrated interdisciplinary effort between engineers, geoscientists, and astrobiologists. An important tool with which to foster cross-disciplinary work at an early stage of mission preparation is the virtual testbed. In this article, we report on recent progress in the development of an ice transit and performance model for later integration in such a virtual testbed. We introduce a trajectory model that, for the first time, allows for the evaluation of mission-critical parameters, such as transit time and average/overall power supply. Our workflow is applied to selected, existing cryobot designs while taking into consideration different terrestrial, as well as extraterrestrial, deployment scenarios. Specific analyses presented in this study show the tradeoff minimum transit time and maximum efficiency of a cryobot and allow for quantification of different sources of uncertainty to cryobot's trajectory models.


Assuntos
Meio Ambiente Extraterreno , Júpiter , Gelo , Planeta Terra , Exobiologia , Planetas
3.
Violence Against Women ; : 10778012221140135, 2022 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-36452985

RESUMO

As scholars explore anti-carceral approaches to gender violence, they question how to structure interactive practices to transform, rather than reproduce, dynamics of inequality and harm. Drawing on ethnographic fieldwork conducted with frontline workers in north India, this article argues that the concept of language ideologies can help analyze how interactive practices address gender violence. Language ideologies are shared representations of how interaction connects speaker, audience, and context. By tracking the language ideologies that inform anti-violence interventions, researchers can better analyze the extraordinarily complex semiotic labor required to address gender violence.

4.
Landslides ; 19(12): 2853-2869, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36338899

RESUMO

Rock avalanches can be a significant hazard to communities located in mountainous areas. Probabilistic predictions of the 3D impact area of these events are crucial for assessing rock avalanche risk. Semi-empirical, calibration-based numerical runout models are one tool that can be used to make these predictions. When doing so, uncertainties resulting from both noisy calibration data and uncertain governing movement mechanism(s) must be accounted for. In this paper, a back-analysis of a database of 31 rock avalanche case histories is used to assess both of these sources of uncertainty. It is found that forecasting results are dominated by uncertainties associated with the bulk basal resistance of the path material. A method to account for both calibration and mechanistic uncertainty is provided, and this method is evaluated using pseudo-forecasts of two case histories. These pseudo-forecasts show that inclusion of expert judgement when assessing the bulk basal resistance along the path can reduce mechanistic uncertainty and result in more precise predictions of rock avalanche runout. Supplementary Information: The online version contains supplementary material available at 10.1007/s10346-022-01939-y.

5.
J Magn Reson ; 220: 32-44, 2012 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-22683579

RESUMO

In this paper we consider low Péclet number flow in bead packs. A series of relaxation exchange experiments has been conducted and evaluated by ILT analysis. In the resulting correlation maps, we observed a collapse of the signal and a translation towards smaller relaxation times with increasing flow rates, as well as a signal tilt with respect to the diagonal. In the discussion of the phenomena we present a mathematical theory for relaxation exchange experiments that considers both diffusive and advective transport. We perform simulations based on this theory and discuss them with respect to the conducted experiments.


Assuntos
Vidro/química , Espectroscopia de Ressonância Magnética/métodos , Modelos Químicos , Reologia/métodos , Simulação por Computador , Microesferas
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