A Novel Mastadenovirus from Nyctalus noctula Which Represents a Distinct Evolutionary Branch of Viruses from Bats in Europe.

Bats are natural hosts of a wide variety of viruses, including adenoviruses. European bats are known to carry mastadenoviruses categorized as species B (widespread in European Vespertilionidae bats) and whose taxonomy has not been clarified. We examined fecal samples from Vespertilionidae bats (five species) captured in central Russia and found that 2/12 (16%) were positive for mastadenoviruses. The partial genome of the mastadenovirus was assembled from Pipistrellus nathusii, representing the bat adenovirus species B. The complete genome (37,915 nt) of a novel mastadenovirus was assembled from Nyctalus noctula and named BatAdV/MOW15-Nn19/Quixote. Comparative studies showed significant divergence of the Quixote genome sequence from European bat mastadenoviruses, while the only known virus showing low similarity was the isolate WA3301 from an Australian bat, and together they formed a subclade that separated from other BatAdVs. Phylogenetic and comparative analysis of the protein-coding genes provided evidence that Quixote is related to a novel species within the genus Mastadenovirus, provisionally named "K" (as the next available letter for the species). Phylogenetic analyses revealed that some earlier viruses from Western European bats, for which only partial DNA polymerase genes are known, are most likely members of the tentatively named species "K". Thus, at least two species of mastadenovirus are circulating in bats throughout Europe, from western to eastern areas.

Taxonomic diversity of the Hipposideros larvatus species complex (Chiroptera: Hipposideridae) in mainland Asia.

Hipposideros larvatus sensu lato constitutes a widespread species complex consisting of morphologically similar forms with a taxonomy that is in need of a revision. Here we present the results of a phylogenetic analysis of members of this species complex based on two mitochondrial (CytB and COI) and seven nuclear (ABHD11, ACOX2, COPS, RAG2, ROGDI2, SORBS2 and THY) genes, and a morphometric analysis. Morphological data, although showing certain diversity, does not allow an accurate delimitation of the taxonomic boundaries and occasionally contradict to genetic grouping. The molecular analyses based on mitochondrial data support the paraphyly of the H. larvatus species complex, with H. armiger recovered nested within. The nuclear data recovered all the lineages of H. larvatus sensu lato forming a monophyletic clade. Within it, apparently four different species-level clades could be revealed in Southeast Asia. One is distributed in northern Myanmar, southern China (including Hainan) and northern Vietnam, and also reaches into central Vietnam along the coast (Cham Islands). The valid name for this taxon must be H. poutensis Allen. The second clade is comprised by populations inhabiting the Malay Peninsula and the Sunda Islands, we identify this taxon as H. larvatus sensu stricto. The third taxon was identified only from southern Myanmar. The fourth taxon is distributed in southern and central Indochina. Its representatives were not related to any of the forms identified in Myanmar, and therefore, its previous identification with H. grandis sounds questionable. Since both the third and fourth taxa cannot now be unambiguously associated with a particular named form, we conventionally designate them as H. cf. grandis 1 and 2.

Back to life and to taxonomy: new record and reassessment of Myotis bucharensis (Chiroptera: Vespertilionidae).

Myotis bucharensis is one of the least studied Palaearctic bat species, known from only three localities in Tajikistan and Uzbekistan and not reported since 1965. In autumn 2019, a male Myotis captured in Zerafshan river basin in Tajikistan was later identified as M. bucharensis based on tail and tibia proportions and strongly displacement of posterior small premolars. The identification was then confirmed by morphometric analyses supporting that M. bucharensis is specifically different but represents a part of the Myotis frater complex. Analyses of one mitochondrial (cyt b) and one nuclear gene (RAG2) were performed for the first time for M. bucharensis. According to these genetic results, this form is indeed a member of the «daubentonii¼ clade, which includes all known frater-like Myotis, and most likely represents a sister species to M. longicaudatus. Record of the alive specimen M. bucharensis has valuable implication for bat conservation in Tajikistan.

A new genus of vespertilionid bat: the end of a long journey for Joffre's Pipistrelle (Chiroptera: Vespertilionidae).

Knowledge as to the taxonomic status of enigmatic bat species often is hindered by limited availability of specimens. This is particularly true for aerial-hawking bats that are difficult to catch. One such species, "Hypsugo" joffrei, was originally described in Nyctalus due to its long and slender wings, but subsequently transferred to Pipistrellus, and most recently to Hypsugo, on the basis of morphology. Analysis of newly available material, which more than doubles the known specimens of this taxon, demonstrates that it is morphologically and genetically distinct from all other bat genera. We accordingly describe it as belonging to a new, monotypic genus. We provide a detailed description of its external and craniodental traits, measurements, and assessment of genetic relationships, including barcode sequences to facilitate its rapid identification in future. The new genus belongs to a group that includes the recently described Cassistrellus, as well as Tylonycteris, and its closest relative, Philetor. We also describe the echolocation calls emitted by members of the taxon in different situations, which may facilitate finding them in previously unsampled locations. Based on the new data, the species occurs from Nepal to North Vietnam and China, which suggests that it could be more widespread than previously thought.

Taxonomic status of the barbastelles (Chiroptera: Vespertilionidae: Barbastella) from the Japanese archipelago and Kunashir Island.

Until recently, almost all barbastelle populations inhabiting Asia, from Iran to Japan and Taiwan, were recognized as a single species, Barbastella darjelingensis. However, in recent years, it was shown that the taxonomic diversity within the genus Barbastella was underestimated, and B. darjelingensis sensu lato is highly variable, both morphologically and genetically. Transcaucasian and Central Asian populations were already raised to the species level as B. caspica. Populations from eastern and south-eastern Asia are still understudied and likely encompass undescribed species. Comparing results from mtDNA sequences, skull morphometry and baculum shape, we concluded that one of these putative species is represented by populations inhabiting Honshu, Hokkaido and Kunashir Islands, and provide a scientific description of this new species. Populations from Taiwan, southern China and Indochina represent a sister mitochondrial clade to this new species. However, available morphological material from these areas did not demonstrate a significant difference from the Himalayan B. darjelingensis. Therefore, we refuse to give this form any taxonomic status until further studies of more vast material are done.

Unusual Pipistrelle: Taxonomic Position of the Malayan Noctule (Pipistrellus stenopterus; Vespertilionidae; Chiroptera).

Sergei V. Kruskop, Evgeniya N. Solovyeva, and Anna D. Kaznadzey (2018) Despite huge progress in the systematics of bats and, in particular, of the Vespertilionidae family in latest years, the taxonomic position of a number of remarkable bat species has been uncertain until now, partly because of limits in acceptable comparative material. Researchers have previously placed the Malayan noctule, Pipistrellus stenopterus, into Nyctalus, because of similar body shape and proportions, or into Pipistrellus, based on karyological analysis. This study reassesses Pipistrellus stenopterus using available collection material and compares it to various members of Nyctalus and Pipistrellus, as well as with some other related and similar genera, based on respective morphological and molecular genetic features. This species demonstrates vast morphological peculiarities compared to other Pipistrellus-like bats. Nonetheless, both mitochondrial and nuclear genetic markers unequivocally place it close to other Asian pipistrelles, most probably in a sister position to the "javanicus" species group. We propose establishing P. stenopterus as a separate subgenus, Alionoctula. Our results also confirm that Pipistrellus is paraphyletic in its current state, and we suggest that further studies explore its internal taxonomy and limits.

The role of DNA barcodes in understanding and conservation of mammal diversity in southeast Asia.

BACKGROUND: Southeast Asia is recognized as a region of very high biodiversity, much of which is currently at risk due to habitat loss and other threats. However, many aspects of this diversity, even for relatively well-known groups such as mammals, are poorly known, limiting ability to develop conservation plans. This study examines the value of DNA barcodes, sequences of the mitochondrial COI gene, to enhance understanding of mammalian diversity in the region and hence to aid conservation planning. METHODOLOGY AND PRINCIPAL FINDINGS: DNA barcodes were obtained from nearly 1900 specimens representing 165 recognized species of bats. All morphologically or acoustically distinct species, based on classical taxonomy, could be discriminated with DNA barcodes except four closely allied species pairs. Many currently recognized species contained multiple barcode lineages, often with deep divergence suggesting unrecognized species. In addition, most widespread species showed substantial genetic differentiation across their distributions. Our results suggest that mammal species richness within the region may be underestimated by at least 50%, and there are higher levels of endemism and greater intra-specific population structure than previously recognized. CONCLUSIONS: DNA barcodes can aid conservation and research by assisting field workers in identifying species, by helping taxonomists determine species groups needing more detailed analysis, and by facilitating the recognition of the appropriate units and scales for conservation planning.