Effect of inoculation with arbuscular mycorrhizal fungi and blanching on the bioaccessibility of heavy metals in water spinach (Ipomoea aquatica Forsk.).

A plant's tolerance to heavy metals (HMs) and its detoxification mechanisms are associated with the subcellular distribution of HMs and their chemical forms. In this study, water spinach (Ipomoea aquatica Forsk.) was grown in two soils contaminated with a single HM (cadmium, Cd) or combined HMs (Cd and nickel, Ni). Inoculation of arbuscular mycorrizal fungi (AMF) was conducted to increase the accumulation of phosphorus (P) in plants. One major exception was to decrease the migration and accumulation of HMs in edible parts by the formation of P-HM complexes. The effects of blanching and simulated digestion on bioaccessibility were also assessed. The experimental results showed that the water spinach species used in this study had a high capacity to accumulate HMs. AMF treatment improved water spinach growth and decreased the accumulation of Ni but not that of Cd. Soluble and inorganic Cd and Ni were the major subcellular fractions and chemical forms in water spinach; these two HMs also exhibited higher migration capacities in comparison to chromium (Cr). Relative to raw tissues, 45-84% of Cd, Cr, and Ni were leached after blanching. Approximately 32-55%, 16-50%, and 27-40% of Cd, Cr, and Ni, respectively, were bioaccessible and could be metabolized by in vitro digestive fluids.

Cadmium Uptake by Cuttings of Impatiens walleriana in Response to Different Cadmium Concentrations and Growth Periods.

Impatiens walleriana (I. walleriana), a potential cadmium (Cd) hyperaccumulator, can propagate by cuttings, which are less expensive to grow than seedlings. Different growth periods for cuttings, however, may lead to different physiological characteristics. In this study, I. walleriana cuttings were hydroponically grown in Cd-containing solutions (1.0-10.0 µM) for various growth periods (10-60 days). Experimental results showed that the Cd treatments had negative effects on growth compared to the controls that were not spiked with Cd. The extension of the growth period promoted most of the growth exhibitions of I. walleriana, except for SPAD readings for cuttings grown in the 5.0 and 10.0 µM solutions. The accumulation of Cd also increased over time, except in the roots of the cuttings grown in the 5.0 and 10.0 µM solutions. The subcellular distribution and chemical forms of Cd showed that I. walleriana developed better tolerance and detoxification capacities in the cuttings grown in the 5.0 and 10.0 µM solutions than in the cuttings grown in the other two Cd treatments.

Chemical Forms and Health Risk of Cadmium in Water Spinach Grown in Contaminated Soil with an Increased Level of Phosphorus.

(1) Background: Even in croplands with a low concentration of cadmium (Cd), there is still a risk for planting crops because of the high accumulation capacity of some leafy vegetables. (2) Methods: In this study, water spinach was planted in four main soil series (Wa, Eh, Tk, and Yu) in central Taiwan, which were spiked with Cd. The soil available phosphorous content was increased to 10-17 mg/kg, and the accumulation and developed chemical forms of Cd were analyzed. (3) Results: The experimental results showed that addition of phosphorous to Wa and Eh promoted the growth of water spinach. Accumulation and upward translocation of Cd were also increased in the phosphorus treatment compared with the control. The addition of phosphorus increased the percentage of Cd compartmentalized in undissolved Cd phosphate, which revealed that the mobility and toxicity of Cd were reduced in the phosphorus treatment. However, most of the water spinach was not edible because the vegetable-induced hazard quotient, which was calculated using three methods, showed hazardous potential in general.