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Mulch coverage of mining tailings can create anaerobic conditions and consequently establish an anoxic environment that promotes the metabolic processes of anaerobic microorganisms. This anoxic environment has the potential to decrease heavy metal mobility and bioavailability. While tailings exposed to sunlight have been extensively studied, research on the effects of microbial-mediated geochemical cycling of heavy metals in mulch-covered tailings is scarce. This study aimed to examine the effects of mulch coverage-induced alterations in the structures of tailing microbial communities on the biogeochemical processes associated with heavy metals. Mulch coverage significantly reduced the pH of the tailings and the tailings exhibited heavy metal bioavailability. Random forest analysis demonstrated that mulch coverage-induced changes in the As/Cd-contaminated fractions and nutrients (total organic carbon and total nitrogen) were the most crucial predictors of microbial diversity and ecological clusters in the tailings. Notably, different from direct metal(loid) immobilization, mulch coverage can facilitate heavy metal immobilization in tailings by promoting microbial-mediated Fe, S, and As reduction. Overall, this study demonstrated that mulch coverage of tailings contributed to a reduction in heavy metal mobilization, which can be attributed to shifts in microbial-mediated Fe, S, and As reduction processes.The study provides valuable insights into the potential of mulch coverage as a remediation strategy and underscores the importance of microbial-mediated processes in managing heavy metal pollution in tailing systems.
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Tungsten (W) is a critical material that is widely used in military applications, electronics, lighting technology, power engineering and the automotive and aerospace industries. In recent decades, overexploitation of W has generated large amounts of mine waste rocks, which generate elevated content of toxic elements and cause serious adverse effects on ecosystems and public health. Microorganisms are considered important players in toxic element migrations from waste rocks. However, the understanding of how the microbial community structure varies in W mine waste rocks and its key driving factors is still unknown. In this study, high-throughput sequencing methods were used to determine the microbial community profiles along a W content gradient in W mine waste rocks. We found that the microbial community structures showed clear differences across the different W levels in waste rocks. Notably, arsenic (As), instead of W and nutrients, was identified as the most important predictor influencing microbial diversity. Furthermore, our results also showed that As is the most important environmental factor that regulates the distribution patterns of ecological clusters and keystone ASVs. Importantly, we found that the dominant genera have been regulated by As and were widely involved in As biogeochemical cycling in waste rocks. Taken together, our results have provided useful information about the response of microbial communities to W mine waste rocks.
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Arsênio , Microbiota , TungstênioRESUMO
River water quality is closely related to the major ion sources and hydrological conditions. However, there is a limited cognition about the geochemical sources and the seasonal variations of major ions. Thus, in this study, a total of 90 water samples were collected from the Longjiang River and its three tributaries in the dry and wet seasons. The samples were analyzed, including major ion concentrations and physicochemical parameters. Statistical analysis, such as correlation analysis and principal component analysis (PCA), was employed to investigate the spatial and seasonal variations in major ion composition and their respective sources. Our study revealed that the predominant major ions in the studied samples are Ca2+, Mg2+, HCO - 3, and SO2 - 4. Most of ions exhibited notable spatial disparities attributable to variations in geological settings and human activities. Regions characterized by igneous rock outcrops tend to exhibit higher levels of K+ and Na+, while areas with higher population densities in the middle and downstream segments show elevated concentrations of Cl-, NO - 3, SO2 - 4, Na+, and K+. The observed peak SO2 - 4 levels may be attributed to active mining operations. Most parameters displayed higher values in flood season than those in dry season due to dilution effects. Stoichiometric analysis indicated that carbonate weathering inputs contribute to over 85% of the mean total cation concentrations in the water, followed by contributions from silicates, atmospheric deposition, and anthropogenic inputs. On the whole, although the water quality remains non-polluted and is suitable for drinking and irrigation purposes, the enrichment of SO2 - 4 and NO - 3 may contribute to water eutrophication. Caution is warranted during the dry season due to reduced water flow resulting from dam interceptions and limited dilution capacity, potentially leading to elevated pollutant concentrations. Taken together, our results provided a scientific basis for water quality managements of monsoon rivers.
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Monitoramento Ambiental , Íons , Rios , Estações do Ano , Poluentes Químicos da Água , China , Rios/química , Íons/análise , Poluentes Químicos da Água/análise , Qualidade da Água , Análise de Componente PrincipalRESUMO
The growth of pioneer plants in metal mining area soil is closely related to their minimal uptake of toxic elements. Pioneer plants can inhibit the uptake of toxic elements by increasing nutrient uptake. However, few studies have focused on the mechanisms by which the rhizosphere microbiome affect nutrient cycling and their impact on the uptake of toxic elements by pioneer plants. In this study, we selected Blechnum orientale to investigate the potential roles of the rhizosphere microbiome in nutrient cycling and plant growth in a historical tungsten (W) mining area. Our results showed that while the arsenic (As) and W contents in the soil were relatively high, the enrichment levels of As and W in the B. orientale were relatively low. Furthermore, we found that the As and W contents in plants were significantly negatively correlated with soil nutrients (S, P and Mo), suggesting that elevated levels of these soil nutrients could inhibit As and W uptake by B. orientale. Importantly, we found that these nutrients were also identified as the most important factors shaping rhizosphere microbial attributes, including microbial diversity, ecological clusters, and keystone OTUs. Moreover, the genera, keystone taxa and microbial functional genes enriched in the rhizosphere soils from mining areas played a key role in nutrient (S, P and Mo) bioavailability, which could further increase the nutrient uptake by B. orientale. Taken together, our results suggest that rhizosphere microorganisms can improve pioneer plant growth by inhibiting toxic element accumulation via the increase in nutrient cycling in former W mining areas.
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Arsênio , Gleiquênias , Microbiota , Traqueófitas , Arsênio/análise , Tungstênio , Rizosfera , Solo , Plantas , Mineração , Microbiologia do SoloRESUMO
Thallium (Tl) is a nonessential and toxic trace metal that is detrimental to plants, but it can be highly up-taken in green cabbage (Brassica oleracea L. var. capitata L.). It has been proven that there is a significant positive correlation between Tl and Calcium (Ca) contents in plants. However, whether Ca presents a similar role for alleviating Tl toxicity in plants remains unclear, and little is known in terms of evidence for both Ca-enhanced uptake of Tl from soils to green cabbage and associated geochemical processes. In this study, we investigated the influence of Ca in soils on Tl uptake in green cabbage and the associated geochemical process. The pot experiments were conducted in 12 mg/kg Tl(I) and 8 mg/kg Tl(III) treatments with various Ca dosages. The results showed that Ca in soils could significantly enhance Tl uptake in green cabbage, increasing 210% in content over the control group. The soluble concentrations of Tl were largely increased by 210% and 150%, respectively, in 3.0 g/kg Ca treatment, compared with the corresponding treatment without Ca addition. This was attributed to the geochemical process in which the enhanced soluble Ca probably replaces Tl held on the soil particles, releasing more soluble Tl into the soil solution. More interestingly, the bioconcentration factor of the leaves and whole plant for the 2.0, 2.5, 3.0 g/kg Ca dosage group were greatly higher than for the non-Ca treatment, which could reach 207%, implying the addition of Ca can improve the ability of green cabbage to transfer Tl from the stems to the leaves. Furthermore, the pH values dropped with the increasing Ca concentration treatment, and the lower pH in soils also increased Tl mobilization, which resulted in Tl accumulation in green cabbage. Therefore, this work not only informs the improvement of agricultural safety management practices for the farming of crops in Tl-polluted and high-Ca-content areas, but also provides technical support for the exploitation of Ca-assisted phytoextraction technology.
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Brassica , Poluentes do Solo , Tálio/análise , Cálcio/metabolismo , Poluentes do Solo/análise , Brassica/química , Cálcio da Dieta , Solo/química , Intoxicação por Metais Pesados , Plantas/metabolismoRESUMO
A field pilot-scale passive treatment system was developed for in-situ bioremediation of acid mine drainage (AMD). The microbial community and its variation were analyzed. The data proved that 93.7% of total soluble Fe and 99% of soluble Fe(II) could be removed by the system. Principal coordinates analysis (PCoA) showed that a low pH and an elevated Fe concentration within the system created a unique microbial community that was dominated by acidophilic iron-oxidizing bacteria and iron-reducing bacteria. Canonical correlation analysis (CCA) indicated that the pH, iron content and total sulfur jointly determined the composition of the microbial communities. Species of Ferrovum, Delftia, Acinetobacter, Metallibacterium, Acidibacter and Acidiphilium were highly enriched, which promoted the removal of iron. Furthermore, the results revealed important data for the biogeochemical coupling of microbial communities and environmental parameters. These findings are beneficial for further application of in-situ field bioreactors to remediate AMD.
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Microbiota , Mineração , Ácidos , Carvão Mineral , Concentração de Íons de HidrogênioRESUMO
In order to evaluate the pollution status, possible sources, and bioavailability of heavy metals (As, Cd, Pb, Sb, Zn, and Tl), 33 surface sediments were collected from Longjiang River, Southern China. The total concentrations and potential bioavailable concentrations of the heavy metals were analyzed using ICP-MS. Enrichment factors (EFs), Pearson correlation analysis, and principal component analysis (PCA) were used to further assess their pollution degree and potential sources. Results showed that the surface sediments of Longjiang River have been suffering heavy metal (As, Cd, Pb, Sb, and Zn) pollution to different degrees. The maximum concentrations of As, Cd, Pb, Sb, and Zn were 67.0, 7.42, 227, 229, and 807 mg·kg-1, respectively, while the Tl concentration were very low, with little variation. Moreover, the polluted sites were mostly located in the mid-lower of the main stem and in tributaries (Dongxioajiang and downstream of Dahuanjiang), and the pollution degree of the heavy metals, in a descending order, were Cd > Sb > Zn > Pb > As > Tl. Pearson correlation analysis and PCA indicated that As, Cd, Pb, Sb, and Zn predominantly originated from anthropogenic inputs, including nonferrous metal mining and smelting, municipal sewage, and agricultural activities, and Tl mostly derived from natural rock weathering. The bioavailability of heavy metals in the sediments tended to be controlled by their sources. The percentages of bioavailable heavy metals (As, Cd, Pb, Sb, and Zn) in the highly anthropogenic impacted areas (the mid-lower of the main stem and downstream of Dongxiaojiang tributary) were also high, with the average percentages of bioavailable As, Cd, Pb, Sb, and Zn of 26%, 51%, 49%, 38%, and 47%, respectively. High EF values and high bioavailable percentages of heavy metals easily and greatly cause high ecological risk of Longjiang River.
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Disponibilidade Biológica , Sedimentos Geológicos/química , Metais Pesados/análise , Poluentes Químicos da Água/análise , China , Monitoramento Ambiental , Medição de Risco , Rios , Análise EspacialRESUMO
In order to investigate the spatial distribution characteristics of Sb and selected heavy metals, and to discriminate their sources and potential ecological risks in surface sediments of the Duliujiang river,a total of 62 surface sediment samples were collected in this study. Total contents of Sb, As, Cd, Co, Cr, Cu, Mo, Ni, Pb, Tl, Zn and Fe in these samples were measured by inductively coupled plasma mass spectrometry(ICP-MS) and the inductive plasma optical emission spectrometry(ICP-OES). Principal component analysis(PCA) and Pearson correlation analysis were used to deduce the potential sources of these elements. Geo-accumulation index(Igeo), enrichment factor(EF) and Hakanson's potential ecological risk index(Eri and RI) were calculated to evaluate the pollution degree of heavy metals in sediments. The results indicated that the contents of heavy metals in sediments were impacted by human activities to different extents, and the Duliujiang River was significantly contaminated by Sb. The contents of Sb in sediments reached up to 7080 mg·kg-1, and gradually decreased from upstream to downstream, while the contents of As, Cd, Co, Cr, Cu, Mo, Ni, Pb, Tl and Zn varied indistinctively. The PCA results showed that the cumulative proportion of the first two components accounted for 77.67% of the total variables, suggesting that two major sources of Sb and other heavy metals were mining/smelting industry and natural sources. The calculated Igeo and EFs also showed that the surface sediments of the Duliujiang River were majorly polluted by Sb, followed by As and Co, lightly contaminated with Co, Cu, Mo, Ni, Pb and Tl, and uncontaminated with Cr. The ecological hazards(Eri) for each metals in a descending order were Sb > Cd > As > Co > Ni > Pb > Cu > Zn > Cr. The comprehensive index of potential ecological risks(RI) for heavy metals indicated that 58.1% of the 62 sediments samples had more than moderate ecological risks, and the sites with high RIs were generally located around Sb mining area and the downstream of the Baluo River. In addition, the Eri of Sb was a predominant component of RI, indicating that the Duliujiang River is an area with extremely high potential ecological risk of Sb.
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Mining activities have introduced various pollutants to surrounding aquatic and terrestrial environments, causing adverse impacts to the environment. Indigenous microbial communities are responsible for the biogeochemical cycling of pollutants in diverse environments, indicating the potential for bioremediation of such pollutants. Antimony (Sb) has been extensively mined in China and Sb contamination in mining areas has been frequently encountered. To date, however, the microbial composition and structure in response to Sb contamination has remained overlooked. Sb and As frequently co-occur in sulfide-rich ores, and co-contamination of Sb and As is observed in some mining areas. We characterized, for the first time, the microbial community profiles and their responses to Sb and As pollution from a watershed heavily contaminated by Sb tailing pond in Southwest China. The indigenous microbial communities were profiled by high-throughput sequencing from 16 sediment samples (535,390 valid reads). The comprehensive geochemical data (specifically, physical-chemical properties and different Sb and As extraction fractions) were obtained from river water and sediments at different depths as well. Canonical correspondence analysis (CCA) demonstrated that a suite of in situ geochemical and physical factors significantly structured the overall microbial community compositions. Further, we found significant correlations between individual phylotypes (bacterial genera) and the geochemical fractions of Sb and As by Spearman rank correlation. A number of taxonomic groups were positively correlated with the Sb and As extractable fractions and various Sb and As species in sediment, suggesting potential roles of these phylotypes in Sb biogeochemical cycling.