A new glance on root-to-shoot in vivo zinc transport and time-dependent physiological effects of ZnSO4 and ZnO nanoparticles on plants.

Understanding nanoparticle root uptake and root-to-shoot transport might contribute to the use of nanotechnology in plant nutrition. This study performed time resolved experiments to probe Zn uptake, biotransformation and physiological effects on Phaseolus vulgaris (L.). Plants roots were exposed to ZnO nanoparticles (40 and 300 nm) dispersions and ZnSO4(aq) (100 and 1000 mg Zn L-1) for 48 h. Near edge X-ray absorption spectroscopy showed that 40 nm ZnO was more easily dissolved by roots than 300 nm ZnO. It also showed that in the leaves Zn was found as a mixture Zn3(PO4)2 and Zn-histidine complex. X-ray fluorescence spectroscopy showed that root-to-shoot Zn-translocation presented a decreasing gradient of concentration and velocity, it seems radial Zn movement occurs simultaneously to the axial xylem transport. Below 100 mg Zn L-1, the lower stem tissue section served as a buffer preventing Zn from reaching the leaves. Conversely, it was not observed for 1000 mg Zn L-1 ZnSO4(aq). Transcriptional analysis of genes encoding metal carriers indicated higher expression levels of tonoplast-localized transporters, suggesting that the mechanism trend to accumulate Zn in the lower tissues may be associated with an enhanced of Zn compartmentalization in vacuoles. The photosynthetic rate, transpiration, and water conductance were impaired by treatments.

Efeito da aplicação de biocarvão, cama de frango e formulado NPK no estado nutricional foliar de laranjeira em terra mulata / Effect of biochar, poultry litter and NPK on the nutritional status leaf of orange on terra mulata

O cultivo de laranjeira em Terra Mulata tem apresentado limitações nutricionais sendo necessário o uso de adubos nas áreas agrícolas. A aplicação de biocarvão associado à outra fonte de nutrientes pode ser uma alternativa viável para aumentar a eficiência dos adubos e diminuir perdas nestas áreas. O objetivo deste trabalho foi avaliar o efeito do biocarvão, da cama de frango e do formulado NPK, no estado nutricional de laranjeiras Pera-Rio cultivadas em um Latossolo Amarelo distrófico com horizonte A antrópico (Terra Mulata). O estudo foi desenvolvido em uma propriedade rural de terra firme em Manacapuru (AM) em um delineamento de blocos casualizados com oito tratamentos utilizando biocarvão, cama de frango e formulado NPK (4-14-8) aplicados de maneira isolada e associados. Para avaliação foram retiradas duas amostras em períodos distintos de folhas para determinação do estado nutricional. Os adubos aplicados não aumentaram a eficiência na disponibilidade dos teores de N, P, Fe e Zn, ficando este último abaixo da faixa de teor adequado. Já para os teores de K, o adubo cama de frango e suas combinações aumentaram sua disponibilidade, e os maiores teores de Mg foram encontrados no tratamento controle.

Growing in orange on Terra Mulata has been submitted nutrient limitation requiring correction in agricultural areas. The application of biochar associated with another source of nutrients can be a viable alternative to increase the efficiency of fertilizers and reduce losses in the areas. The objective of this study was to evaluate the effect of biochar, poultry litter and NPK in the nutritional status of orange trees grown in an Oxisol with anthropogenic A horizon (Terra Mulata). The study was conducted in a rural property landfall in Manacapuru (AM) in a randomized block design with eight treatments using biochar, poultry litter and NPK (4-14-8) applied in isolation and associates. To review two samples were taken at different periods of sheets to determine the nutritional status. Fertilizers applied did not increase the efficiency in the availability of contents of N, P, Fe and Zn, being the latter below the range of suitable content. As for the K, compost chicken manure and their combinations increased their availability, and the highest levels of Mg were found in the control treatment.