Thermal tolerance of Cyclops kolensis lilljeborg, 1901 (Copepoda: Cyclopoida) and its relationships with pessimal to optimal performance.

Cyclops kolensis Lilljeborg, 1901 belongs to the Arctic complex of Palaeoarctic species, yet in the past 20 years, its occurrence has extended to the summer months in waterbodies with high water temperatures. This species is considered one of the most active migrants from the northern waterbodies in the Volga reservoir cascade to the Volga delta. Here, we explored the ranges of the preferred and avoidance temperatures of C. kolensis from two geographically isolated populations. Thermal tolerance was measured in a thermogradient installation and compared to the temperatures at which members of these populations occurred in their source waterbodies. Temperature preference was determined using the "chronic" method. Individuals of C. kolensis possessed a bimodal final temperature preferendum of 2-6 °C and 13-21 °C, which corresponds to the optimal thermal conditions of the species in a pond. These ranges were the same for individuals of both populations irrespective of the geographical location and water temperature of their source waterbodies. The temperature range of normal performance was 2-4 to 21-25 °C, and the pessimal temperature ranges were from 1 - 2 to 3-4 °C and from 22-25 °C to 26-30°Ð¡. These temperature ranges coincide with field observations over a recent 20-year period of temperature conditions under which the species develops in nature. Our results allow us to characterize C. kolensis as an ecologically plastic species, which, despite its strong association with the cold-water Complex species, is adapting to a wider temperature range as global warming occurs.

The preferred and avoidance temperatures of Thermocyclops crassus (Fischer, 1853) and their relation to the temperature of optimal, pessimal and normal performance of the species.

The ranges of the preferred and avoided temperatures in representatives of Thermocyclops crassus (Fischer, 1853) were determined by the results of experimental testing in a thermogradient aparatus and comparison of the obtained values with field observations of the optimal, pessimal and tolerated temperature conditions of development of these populations in nature. Copepods were sampled from pond located near Borok, Yaroslavl region, Russia (58o02'57'' N; 38o14'56'' E). The ambient water temperature was 15.0°Ð¡. Temperature preference was determined by the "chronic" method. The phenology of development of Th. crassus was observed in the field of water bodies in Central and Volga Federal Districts of Russia. The final thermal preferendum (FTP) achievement occurred with an increase in the preferred temperature: when animals were placed at a temperature in the thermal gradient (14.0-15.0°C) that approximated the temperature of the source pond, they moved to warmer water until they chose FTP (26.7°C). Obtained the values of FTP (25-30°C), temperature of normal performance (21-32°C) and pessimal temperatures of 9-20 and 33°Ð¡ well coincide with numerous field observations for temperature conditions of development of species in northern waterbodies of Holarctic and with the temperatures at which populations of Th. crassus thrive in southern waterbodies of Holarctic and in tropical lakes. It is concluded that, despite the historically long existence of the species in the reservoirs of the temperate climatic zone, the northern populations of Th. crassus retained the temperature responses characteristic of their southern sister populations. And although the species has adapted to life in northern reservoirs at lower temperatures, when it becomes possible to choose, cyclops prefer temperatures above 25°C, which are optimal for southern populations living in tropical waters. These data once again confirm that the horizontal thermal gradient method can be used to infer temperature tolerance of freshwater cyclopoid copepods in nature.

Phylogenetic information content of Copepoda ribosomal DNA repeat units: ITS1 and ITS2 impact.

The utility of various regions of the ribosomal repeat unit for phylogenetic analysis was examined in 16 species representing four families, nine genera, and two orders of the subclass Copepoda (Crustacea). Fragments approximately 2000 bp in length containing the ribosomal DNA (rDNA) 18S and 28S gene fragments, the 5.8S gene, and the internal transcribed spacer regions I and II (ITS1 and ITS2) were amplified and analyzed. The DAMBE (Data Analysis in Molecular Biology and Evolution) software was used to analyze the saturation of nucleotide substitutions; this test revealed the suitability of both the 28S gene fragment and the ITS1/ITS2 rDNA regions for the reconstruction of phylogenetic trees. Distance (minimum evolution) and probabilistic (maximum likelihood, Bayesian) analyses of the data revealed that the 28S rDNA and the ITS1 and ITS2 regions are informative markers for inferring phylogenetic relationships among families of copepods and within the Cyclopidae family and associated genera. Split-graph analysis of concatenated ITS1/ITS2 rDNA regions of cyclopoid copepods suggested that the Mesocyclops, Thermocyclops, and Macrocyclops genera share complex evolutionary relationships. This study revealed that the ITS1 and ITS2 regions potentially represent different phylogenetic signals.